小酸枣汤对小鼠自主活动及睡眠时间影响的拆方研究
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摘要
目的:考察小酸枣汤对小鼠自主活动及睡眠时间的影响,并进行拆方研究。方法:将小酸枣汤组方中7味药材(酸枣仁、茯苓、川芎、知母、甘草、生姜、桂心)作为实验因素,每个因素选取有或无2个水平,按L_8(2~7)正交表安排试验。采用直观分析法和方差分析法比较小酸枣汤组方和方中各单味药对小鼠2 min内自主活动及阈上剂量戊巴比妥钠致小鼠睡眠时间的影响。结果:小酸枣汤组方中对小鼠自主活动具有明显影响作用的是酸枣仁和知母;对阈上剂量戊巴比妥钠所致小鼠睡眠时间有明显影响的是酸枣仁。结论:小酸枣汤具有抑制小鼠自主活动及延长阈上剂量戊巴比妥钠致小鼠睡眠时间的作用,方中酸枣仁作用最强。
OBJECTIVE:To investigate the effects of Xiaosuanzao decoction on spontaneous motion and sleep time in mice,and to study its disassembled formulation. METHODS:Seven ingredients of Xiaosuanzao decoction were selected as the influential factors,including Ziziphus jujuba,Poria cocos,Ligusticum chuanxiong,Anemarrhena asphodeloides,Glycyrrhiza uralensis,Zingiber officinale and Cortex cinnamoni. Two levels of with or without were designed for each factor,and the experiment was arranged according to L_8(2~7)orthogonal test table. The effects of Xiaosuanzao decoction and each ingredient on spontaneous motion within 2 min and sleep time induced by superthreshold dosage of pentobarbital sodium were compared by visual analysis method and analysis of variance. RESULTS:Z. jujube and A. asphodeloides were the main active ingredients of Xiaosuanzao decoction on spontaneous motion;Z. jujube was the main active ingredient of Xiaosuanzao decoction on sleep time induced by superthreshold dosage of pentobarbital sodium. CONCLUSIONS:Xiaosuanzao decoction can inhibit spontaneous motion and prolong sleep time induced by superthreshold dosage of pentobarbital sodium,and Z. jujube is the main ingredient of Xiaosuanzao decoction.
OBJECTIVE:To investigate the effects of Xiaosuanzao decoction on spontaneous motion and sleep time in mice,and to study its disassembled formulation. METHODS:Seven ingredients of Xiaosuanzao decoction were selected as the influential factors,including Ziziphus jujuba,Poria cocos,Ligusticum chuanxiong,Anemarrhena asphodeloides,Glycyrrhiza uralensis,Zingiber officinale and Cortex cinnamoni. Two levels of with or without were designed for each factor,and the experiment was arranged according to L_8(2~7)orthogonal test table. The effects of Xiaosuanzao decoction and each ingredient on spontaneous motion within 2 min and sleep time induced by superthreshold dosage of pentobarbital sodium were compared by visual analysis method and analysis of variance. RESULTS:Z. jujube and A. asphodeloides were the main active ingredients of Xiaosuanzao decoction on spontaneous motion;Z. jujube was the main active ingredient of Xiaosuanzao decoction on sleep time induced by superthreshold dosage of pentobarbital sodium. CONCLUSIONS:Xiaosuanzao decoction can inhibit spontaneous motion and prolong sleep time induced by superthreshold dosage of pentobarbital sodium,and Z. jujube is the main ingredient of Xiaosuanzao decoction.
引文
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[5]李玉娟,刘雯,杨静玉,等.酸枣仁汤的镇静催眠作用[J].沈阳药科大学学报,2002,19(2):115-117.
[6]刘莹,陈晓伟,李健,等.茵陈蒿汤拆方对非酒精性脂肪性肝炎的正交实验研究[C]//第二十五届全国中西医结合消化系统疾病学术会议论文集.北京:中国中西医结合学会,2013:182-188.
[7]汪海鸿,狄留庆,陆茵,等.通塞脉片治疗急性脑缺血的拆方研究[J].南京中医药大学学报,2011,27(1):55-57.
[8]张继平,刘俊娥,姚晖,等.正交试验法研究补阳还五汤中保护OGD损伤PC12细胞的主要药味[J].中国实验方剂学杂志,2014,20(11):134-138.
[9]龙旭阳,郑攀.方剂拆方研究现状[J].浙江中医药大学学报,2010,34(1):120-121.
[10]徐智,薛梅苓,单升高,等.中药及其成分配伍组方的研究方法探析[J].中国卫生产业,2013,10(20):86-87.
[11]陈奇,邓文龙,张世玮,等.中药药理研究方法学[M].北京:人民卫生出版社,1994:67.
[12]封硕.酸枣仁汤君臣佐使配伍的药效学研究[D].沈阳:辽宁中医药大学,2007.
[13]沈鸿,黄芳,窦昌贵,等.酸枣仁汤处方的配比研究[J].中国药科大学学报,2002,33(1):21-23.
[14]詹爱萍,王平,陈科力.半夏、掌叶半夏和水半夏对小鼠镇静催眠作用的比较研究[J].中药材,2006,29(9):964-965.
[15]谭云龙,孙晖,孙文军,等.酸枣仁化学成分及其药理作用研究进展[J].时珍国医国药,2014,25(1):186-188.
[16]张妍,解军波,张彦青,等.镇静催眠药物的主要作用机制研究进展[J].中国药房,2011,22(41):3906-3908.
[17]崔思娇,贾英,罗洁,等.酸枣仁镇静催眠活性部位的筛选[J].中国药房,2013,24(23):2128-2129.