大黄虫丸全方及君药药对中主要药效成分的定性定量研究
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摘要
目的借助高效液相色谱分析技术,利用中药复方拆方的研究思路,比较分析大黄虫丸全方及大黄、土鳖虫药对中药效成分变化。方法色谱柱为Thermo Hypersil Gold;流动相0.1%甲酸水-甲醇,梯度洗脱;柱温:30℃;检测波长270nm,建立大黄虫丸及其君药药对指纹图谱,定量分析君药大黄中蒽醌类成分以及土鳖虫中次黄嘌呤含量。结果分别建立大黄虫丸全方及药对的液相指纹图谱,全方共鉴定了32个共有峰,药对鉴定了27个。在定量研究中,我们发现药对中5种大黄蒽醌的含量均比全方高,其中芦荟大黄素1.6倍,大黄酸1.48倍,大黄素2.23倍,大黄酚2.43倍,大黄素甲醚2.48倍,次黄嘌呤含量较全方低。结论大黄虫丸全方及药对均具有抗肝癌作用,虽然药对中大黄蒽醌类成分较全方高,但抗肝癌作用临床上以全方为优,提示全方低剂量多成分、多靶点作用的重要性。对大黄虫丸中蒽醌类以及动物药中次黄嘌呤含量的测定提供了参考,也为后期进一步探索全方抗肝癌物质基础提供思路。
OBJECTIVE To comparative analysis of the changes of pharmacodynamics between Dahuang Zhechong Pills and rhubarb pairing with eupolyphaga by means of high performance liquid chromatography(HPLC),using the research ideas of traditional Chinese medicine compound prescriptions.METHODS The chromatographic column was Thermo Hypersil Gold,mobile phase 0.1%formic acid water-methanol,gradient elution,column temperature:30℃,detection wavelength 270 nm,to establish the fingerprints of Dahuang Zhechong Pills and main medicine paris,and to quantitatively analyze the content of anthraquinones in rhubarb as well as hypoxanthine.RESULTS Establish liquid fingerprint respectively in Dahuang Zhechong Pills and rhubarb pairing with eupolyphaga.A total of 32 common peaks were identified and 27 drugs were identified.In the quantitative study,we found that the content of five kinds of rhubarb anthraquinone in the drug was higher than that of the whole body,including 1.6 times of aloe-emodin,1.48 times of rhein,2.23 times of emodin,2.43 times of chrysophanol,2.48 times of physcion,hypoxanthine content was more than the whole group.CONCLUSION Pairs and whole group of Dahuang Zhechong Pills both have a certain anti-liver cancer effect.Although drugs on the component anthraquinones are higher than the whole side,the role of anti-liver cancer to the whole for the excellent,suggesting that the whole low-dose multi-component,multi-target role in the importance.In this study,we provide reference for the determination of anthraquinone and hypoxanthine in Dahuangzhechong pills and provide the idea for further exploring the material basis of liver cancer.
OBJECTIVE To comparative analysis of the changes of pharmacodynamics between Dahuang Zhechong Pills and rhubarb pairing with eupolyphaga by means of high performance liquid chromatography(HPLC),using the research ideas of traditional Chinese medicine compound prescriptions.METHODS The chromatographic column was Thermo Hypersil Gold,mobile phase 0.1%formic acid water-methanol,gradient elution,column temperature:30℃,detection wavelength 270 nm,to establish the fingerprints of Dahuang Zhechong Pills and main medicine paris,and to quantitatively analyze the content of anthraquinones in rhubarb as well as hypoxanthine.RESULTS Establish liquid fingerprint respectively in Dahuang Zhechong Pills and rhubarb pairing with eupolyphaga.A total of 32 common peaks were identified and 27 drugs were identified.In the quantitative study,we found that the content of five kinds of rhubarb anthraquinone in the drug was higher than that of the whole body,including 1.6 times of aloe-emodin,1.48 times of rhein,2.23 times of emodin,2.43 times of chrysophanol,2.48 times of physcion,hypoxanthine content was more than the whole group.CONCLUSION Pairs and whole group of Dahuang Zhechong Pills both have a certain anti-liver cancer effect.Although drugs on the component anthraquinones are higher than the whole side,the role of anti-liver cancer to the whole for the excellent,suggesting that the whole low-dose multi-component,multi-target role in the importance.In this study,we provide reference for the determination of anthraquinone and hypoxanthine in Dahuangzhechong pills and provide the idea for further exploring the material basis of liver cancer.
引文
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[2]冬梅,杨海厚.大黄蜇虫丸异病同治举隅[J].新疆中医药,2009,27(5):81-83.
[3]乌云达来,萨仁高娃.大黄虫丸临床研究进展[J].世界最新医学信息文摘,2016,16(97):70-71.
[4]聂开平.大黄虫丸临床应用研究综述[J].中国民族民间医药,2010(15):8-9.
[5]吕祥,李柏.中药复方拆方研究进展[J].中国中医药信息杂志,2008,15(10):105-106.
[6]WU L,ZHAO J,CAI H,et al.Dahuang Zhechong Pill combined with doxorubicin induces cell death through regulating energy metabolism in human hepatocellular carcinoma cells[J].Evid-Based Complement Alternat Med,2017(10):1-8.