超临界CO_2中固定化脂肪酶催化鸡油水解的工艺研究
摘要
文章研究了超临界CO_2中固定化脂肪酶催化鸡油水解反应的效果。通过单因素实验考察了酶用量、温度、底物浓度、pH、搅拌转速对酶解率的影响,在此基础上采用响应面实验对温度、反应时间、酶量、搅拌转速工艺参数进行优化。实验结果表明:超临界CO_2中固定化脂肪酶催化鸡油水解的最佳条件为酶量0.45%、温度42.5℃、搅拌转速100 rpm、反应时间106.401 min,水解率为85.84%。
引文
[1]盛梅,曹国民蚕丝固定化脂肪酶催化猪油水解[J]中国油脂,1999(4):36-38
[2]曹国民,盛梅,高广达脂肪酶催化动物脂肪水解[J]中国油脂,1997(2):29-30
[3]Adamczak M,Bednarski W Enhanced activity of intracellular lipases from Rhizomucor miehei and Yarrowia lipolytica by immobilization on biomass support particles[J]Process Biochemistry,2004(39):1347-1361
[4]Jackson M A,King J W Lipase-catalyzed glycerolysis of soybean oil in supercritical carbon dioxide[J]J Am Oil Chem Soc,1997,74(2):103-106
[5]Holmberg K,Lassen B,Stark M B Enzymatic glycerolysis of a triglyceride in aqueous and nonaqueous microemulsions[J]J Am Oil Chem Soc,1989,66(12):1796-1800
[6]GB/T 5534-1995,动植物油脂皂化值的测定[S]
[7]M Primozic,M Habulin,Z Knez Parameter optimization for the enzymatic hydrolysis of sunflower oil in high-pressure reactors[J]JAOCS,2003,80(7):643-646
[8]C Turner,M Persson,L Mathiasson,et al Lipasecatalyzed reactions in organic and supercritical solvents:application to fat-soluble vitamin determination in milk powder and infant formula[J]Enzyme and Microbial Technology,2001(29):111-121
[9]M Habulin,Z Knez High-pressure enzymatic hydrolysis of oil.Eur.J.Lipid Sci.Technol,2002(104):381-386
[10]C Turner,J W King,T Mckeon Selected uses of enzymes with critical fluids in analytical chemistry[J]Journal of AOAC International,2004,87(4):797-810
[11]N K Guthalugu,M Balaraman,U S Kadimi Optimization of enzymatic hydrolysis of triglycerides in soy deodorized distillate with supercritical carbon dioxide Biochemical Engineering Journal,2006(29):220-226
[12]S V Kamat,E J Beckman,A J Russell Enzyme activity in supercritical fluids[J]Critical Reviews in Biotechnology,1995,15(1):41-71
[13]Yao Yan,K Nagahama Activity of free candida rugosa lipase in hydrolysis reaction of tuna oil under high pressure carbon dioxide[J]Journal of Chemical Engineering of Japan,2003,36(5):557-562
[14]H Sovova,M Zarevucka Lipase-catalysed hydrolysis of blackcurrant oil in supercritical carbon dioxide[J]Chemical Engineering Science,2003(58):2339-2350
[15]A J Mesiano,E J Beckman,A J Russell Supercritical biocatalysis[J]Chemical reviews,1999(99):623-633
[2]曹国民,盛梅,高广达脂肪酶催化动物脂肪水解[J]中国油脂,1997(2):29-30
[3]Adamczak M,Bednarski W Enhanced activity of intracellular lipases from Rhizomucor miehei and Yarrowia lipolytica by immobilization on biomass support particles[J]Process Biochemistry,2004(39):1347-1361
[4]Jackson M A,King J W Lipase-catalyzed glycerolysis of soybean oil in supercritical carbon dioxide[J]J Am Oil Chem Soc,1997,74(2):103-106
[5]Holmberg K,Lassen B,Stark M B Enzymatic glycerolysis of a triglyceride in aqueous and nonaqueous microemulsions[J]J Am Oil Chem Soc,1989,66(12):1796-1800
[6]GB/T 5534-1995,动植物油脂皂化值的测定[S]
[7]M Primozic,M Habulin,Z Knez Parameter optimization for the enzymatic hydrolysis of sunflower oil in high-pressure reactors[J]JAOCS,2003,80(7):643-646
[8]C Turner,M Persson,L Mathiasson,et al Lipasecatalyzed reactions in organic and supercritical solvents:application to fat-soluble vitamin determination in milk powder and infant formula[J]Enzyme and Microbial Technology,2001(29):111-121
[9]M Habulin,Z Knez High-pressure enzymatic hydrolysis of oil.Eur.J.Lipid Sci.Technol,2002(104):381-386
[10]C Turner,J W King,T Mckeon Selected uses of enzymes with critical fluids in analytical chemistry[J]Journal of AOAC International,2004,87(4):797-810
[11]N K Guthalugu,M Balaraman,U S Kadimi Optimization of enzymatic hydrolysis of triglycerides in soy deodorized distillate with supercritical carbon dioxide Biochemical Engineering Journal,2006(29):220-226
[12]S V Kamat,E J Beckman,A J Russell Enzyme activity in supercritical fluids[J]Critical Reviews in Biotechnology,1995,15(1):41-71
[13]Yao Yan,K Nagahama Activity of free candida rugosa lipase in hydrolysis reaction of tuna oil under high pressure carbon dioxide[J]Journal of Chemical Engineering of Japan,2003,36(5):557-562
[14]H Sovova,M Zarevucka Lipase-catalysed hydrolysis of blackcurrant oil in supercritical carbon dioxide[J]Chemical Engineering Science,2003(58):2339-2350
[15]A J Mesiano,E J Beckman,A J Russell Supercritical biocatalysis[J]Chemical reviews,1999(99):623-633