肺形草的化学成分研究
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摘要
为研究肺形草的化学成分,采用硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相高效液相制备色谱对该植物的乙酸乙酯层进行分离纯化,通过1H NMR和13C NMR波谱技术进行结构鉴定.结果表明:从乙酸乙酯层中分离得到了8个化合物,分别鉴定为2'-acetamido-3'-phenylpropyl 2-benzamido-3-phenyl propionate(1)、1,3,5-三羟基口山酮(2)、3-羟基-4-甲氧基苯甲酸甲酯(3)、咖啡酸乙酯(4)、邻苯二甲酸二丁酯(5)、1,7-二羟基-3,8-二甲氧基口山酮(6)、morroniside(7)、1,3,7-三羟基-4-C-β-D-葡萄糖基呫吨酮(8).其中化合物1～5为首次从该植物中分离得到.
To investigate the chemical constituents of Tripterospermum chinense,the Et OAc extract of the whole plant were separated and purified by column chromatography with silica gel,Sephadex LH-20 and ODS-HPLC. The structures of the constituents were identified by1 H NMR and13 C NMR. The results indicated that eight compounds were isolated from the extract and identified as 2'-acetamido-3'-phenylpropyl 2-benzamido-3-phenyl propionate( 1),1,3,5-trihydroxyxanthone( 2),3-Hydroxy-4-methoxy-benzoic acid methyl ester( 3),ethyl caffeate( 4),dibutyl phthalate( 5),1,7-dihydroxy-3,8-dimethoxyxanthone( 6),morroniside( 7),lancerin( 8). The compounds 1-5 were isolated from this plant for the first time.
To investigate the chemical constituents of Tripterospermum chinense,the Et OAc extract of the whole plant were separated and purified by column chromatography with silica gel,Sephadex LH-20 and ODS-HPLC. The structures of the constituents were identified by1 H NMR and13 C NMR. The results indicated that eight compounds were isolated from the extract and identified as 2'-acetamido-3'-phenylpropyl 2-benzamido-3-phenyl propionate( 1),1,3,5-trihydroxyxanthone( 2),3-Hydroxy-4-methoxy-benzoic acid methyl ester( 3),ethyl caffeate( 4),dibutyl phthalate( 5),1,7-dihydroxy-3,8-dimethoxyxanthone( 6),morroniside( 7),lancerin( 8). The compounds 1-5 were isolated from this plant for the first time.
引文
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[2]张涛.肺形草抗H5N1禽流感活性成分研究[D].北京:北京工业大学,2013:10-14.
[3]ZHAO X Y,ZHOU X F,RUAN H L,et al.Chemical Constituents of Impatiens pritzellii[J].Chin J Nat Med,2005,3(6):354-356.
[4]郑伟,岳跃栋,龚亚军,等.双斑獐牙菜的化学成分研究[J].中草药,2016,47(9):1468-1476.
[5]PAYNE R J,TOSCANO M D,BULLOCH E M,et al.Design and synthesis of aromatic inhibitors of anthranilate synthase[J].Org Biomol Chem,2005,3(12):2271-2281.
[6]赵东保,张卫,李明静,等.项羽菊化学成分研究[J].中国中药杂志,2006,31(22):1869-1871.
[7]杨光忠,石宽,甘飞,等.飞扬草中酚类成分的分离与鉴定[J].中南民族大学学报(自然科学版),2017,36(1):43-46.
[8]黄伟欢,薛珺一,李药兰,等.了哥王芳香类化学成分研究[J].中药材,2008,31(8):1174-1176.
[9]ZHU K C,MA C,FAN M,et al.Xanthones from Triperospermum chinese(Migo)[J].Asian J Chem,2007,19(3):1739-1742.
[10]GROSS G A,STICHER O.Isosweroside,a new secoiridoid glycoside from the roots of Sambueuseblus(Caprifoliaceae)[J].Hetv Chim Acta,1986,69(5):1113-1119.
[11]MIYASE T,NOGUCHI H,CHEN X M.Sucrose esters and xanthone C-glycosides from the roots of Polygalasibrica[J].J Nat Prod,1999,62(7):993-996.
[12]袁怡.长梗喉毛花保肝活性化学成分研究[D].北京:北京协和医科大学,2010:21-23.