乳酸菌Ⅱ类细菌素生物合成及其代谢调控策略
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摘要
乳酸菌细菌素是乳酸菌经由核糖体合成的具有抑菌活性的多肽类物质,其中乳酸菌Ⅱ类细菌素具有很强的热稳定性和抑菌活性,但是产量较低尚不能实现商业化生产。因此,了解和掌握乳酸菌Ⅱ类细菌素合成及其代谢调控机制,对解决商业化生产过程的技术瓶颈具有重要的意义。该文综述了目前Ⅱ类细菌素生物合成和降解途径,并从代谢调控角度提出提高Ⅱ类细菌素产量的可行性策略,为提高Ⅱ类细菌素产量提供新的途径和思路,也为Ⅱ类细菌素工业化生产奠定基础。
Bacteriocins of lactic acid bacteria are peptides with antimicrobial activity synthesized by ribosome of lactic acid bacteria. Class Ⅱ bacteriocins produced by lactic acid bacteria had strong heat stability and antimicrobial activity, but the yield was too low to realize commercial production.Therefore, understanding the synthesis and metabolic regulation mechanism of class Ⅱ bacteriocins produced by lactic acid bacteria has great importance to the technical bottleneck of commercial production. The biosynthesis and degradation pathway for class Ⅱ bacteriocins were reviewed, some feasible strategies to increase bacteriocins production were proposed. So, the paper provided a new way for increasing bacteriocins production and a certain basis for industrial production of class Ⅱ bacteriocins.
Bacteriocins of lactic acid bacteria are peptides with antimicrobial activity synthesized by ribosome of lactic acid bacteria. Class Ⅱ bacteriocins produced by lactic acid bacteria had strong heat stability and antimicrobial activity, but the yield was too low to realize commercial production.Therefore, understanding the synthesis and metabolic regulation mechanism of class Ⅱ bacteriocins produced by lactic acid bacteria has great importance to the technical bottleneck of commercial production. The biosynthesis and degradation pathway for class Ⅱ bacteriocins were reviewed, some feasible strategies to increase bacteriocins production were proposed. So, the paper provided a new way for increasing bacteriocins production and a certain basis for industrial production of class Ⅱ bacteriocins.
引文
[1]REA M C,ROSS R P,COTTER P D,et al.Classification of bacteriocins from Gram-positive bacteria[M].New York:Springer,2011:29-53.
[2]ZHANG J,YANG Y,YANG H,et al.Purification and partial characterization of bacteriocin Lac-B23,a novel bacteriocin production by Lactobacillus plantarum J23,isolated from Chinese traditional fermented milk[J].Front Microbiol,2018,9(1):2165.
[3]张建志.代谢工程改造大肠杆菌、酪丁酸梭菌生产苯乳酸和正丁醇[D].北京:中国农业大学,2017.
[4]NI Z J,ZHANG X,LIU F,et al.Effect of co-overexpression of nisin key genes on nisin production improvement in Lactococcus lactis LS01[J].Probiot Antimicrob Protein,2017,9(2):204-212.
[5]GUO W L,ZHANG Y B,LU J H,et al.Optimization of fermentation medium for nisin production from Lactococcus lactis subsp.lactis using response surface methodology(RSM)combined with artificial neural network-genetic algorithm(ANN-GA)[J].Afr J Biotechnol,2013,38(9):6264-6272.
[6]DEVI S M,HALAMI P M.Genetic variation of pln loci among probiotic Lactobacillus plantarum group strains with antioxidant and cholesterollowering ability[J].Probiot Antimicrob Protein,2017,1(10):1-12.
[7]SINGH N P,TIWARI A,BANSAL A,et al.Genome level analysis of bacteriocins of lactic acid bacteria[J].Comput Biol Chem,2015,56(6):1-6.
[8]COTTER P D,HILL C,ROSS R P.Bacteriocins:developing innate immunity for food[J].Nat Rev Microbiol,2005,3(10):777-788.
[9]JOHNSEN L,FIMLAND G,NISSEN-MEYER J.The C-terminal domain of pediocin-like antimicrobial peptides(class IIa bacteriocins)is involved in specific recognition of the C-terminal part of cognate immunity proteins and in determining the antimicrobial spectrum[J].J Biol Chem,2005,280(10):9243-9250.
[10]UTENG M,HAUGE H H,MARKWICK P R L,et al.Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and a sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge[J].Biochemistry,2003,42(39):11417-11426.
[11]SPRULE T,KAWULKA K E,GIBBS A C,et al.NMR solution structure of the precursor for carnobacteriocin B2,an antimicrobial peptide from Carnobacterium piscicola[J].Eur J Biochem,2004,271(9):1748-1756.
[12]HAUGEN H S,FUMLAND G,NISSEN-MEYER J,et al.Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide curvacin A[J].Biochemistry,2005,44(49):16149-16157.
[13]SIMON L,FREMAUX C,CENATIEMPO Y,et al.Sakacin G,a new type of antilisterial bacteriocin[J].Appl Environm Microbiol,2002,68(12):6416-6420.
[14]KAUR K,ANDREW L C,WISHART D S,et al.Dynamic relationships among type IIa bacteriocins:temperature effects on antimicrobial activity and on structure of the C-terminal amphipathicαhelix as a receptorbinding region[J].Biochemistry,2004,43(28):9009-9020.
[15]JIMENEZ-DIAZ R,RUIZ-BARBA J L,CATHCART D P,et al.Purification and partial amino acid sequence of plantaricin S,a bacteriocin produced by Lactobacillus plantarum LPCO10,the activity of which depends on the complementary action of two peptides[J].Appl Environm Microbiol,1995,61(12):4459-4463.
[16]MCCORMICK J K,POON A,SAILER M,et al.Genetic characterization and heterologous expression of brochocin-C,an antibotulinal,twopeptide bacteriocin produced by Brochothrix campestris ATCC 43754[J].Appl Environm Microbiol,1998,64(12):4757-4766.
[17]FIMLAND N,ROGNE P,FIMLAND G,et al.Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin plantaricin EF[J].BBA-Protein Proteom,2008,1784(11):1711-1719.
[18]ROGENE P,FIMLAND G,NISSEN-MEYE J,et al.Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin lactococcin G[J].BBA-Protein Proteom,2008,1784(3):543-554.
[19]ROGNE P,HAUGEN C,FIMLAND G,et al.Three-dimensional structure of the two-peptide bacteriocin plantaricin JK[J].Peptides,2009,30(9):1613-1621.
[20]ASPRI M,OCONNOR P M,FIELD D,et al.Application of bacteriocin-producing Enterococcus faecium isolated from donkey milk,in the bio-control of Listeria monocytogenes in fresh whey cheese[J].Int Dairy J,2017,73(10):1-9.
[21]VIGNOLO G,FADDA S,DE KAIRUZ M N,et al.Control of Listeria monocytogenes in ground beef by'Lactocin 705',a bacteriocin produced by Lactobacillus casei CRL 705[J].Int J Food Microbiol,1996,29(2):397-402.
[22]MARTIN-VISSCHER L A,GONG X,DUSZYK M,et al.The three-dimensional structure of carnocyclin A reveals that many circular bacteriocins share a common structural motif[J].J Biol Chem,2009,284(42):28674-28681.
[23]JIMENEZ M,BARRACHI-SACCILOTTO A C,VALDIVIA E,et al.Design,NMR characterization and activity of a 21-residue peptide fragment of bacteriocin AS-48 containing its putative membrane interacting region[J].J Pept Sci,2005,11(1):29-36.
[24]HENG N C K,WESCOMBE P A,BURTON J P,et al.The diversity of bacteriocins in Gram-positive bacteria[M].New York:Springer,2007:45-92.
[25]NISSEN-MEYER J,ROGENE P,OPPEGARD C,et al.Structure-function relationships of the non-lanthionine-containing peptide(class II)bacteriocins produced by gram-positive bacteria[J].Curr Pharmaceut Biotechnol,2009,10(1):19-37.
[26]吴清平,黄静敏,张菊梅,等.细菌素的合成与作用机制[J].微生物学通报,2010,37(10):1519-1524.
[27]QIU J,ZHUO Y,ZHU D,et al.Overexpression of the ABC transporter Avt AB increases avermectin production in Streptomyces avermitilis[J].Appl Microbiol Biot,2011,92(2):337-345.
[28]DRIDER D,FIMLAND G,HECHARD Y,et al.The continuing story of class IIa bacteriocins[J].Microbiol Molr Biol Rev,2006,70(2):564-582.
[29]VENEMA K,KOK J,MARUGG J D,et al.Functional analysis of the pediocin operon of Pediococcus acidilactici PAC1.0:Ped B is the immunity protein and Ped D is the precursor processing enzyme[J].Mol Microbiol,1995,17(3):515-522.
[30]HAVARSTEIN L S,DIEP D B,NES I F.A family of bacteriocin ABCtransporters carry out proteolytic processing of their substrates concomitant with export[J].Mol Microbiol,1995,16(2):229-240.
[31]PAL G,SRIVASTAVA S.In vitro activity of a recombinant ABC transporter protein in the processing of plantaricin E pre-peptide[J].Arch Microbiol,2015,197(6):1-7.
[32]SAVIJOKI K,INGMER H,VARMANEN P.Proteolytic systems of lactic acid bacteria[J].Appl Microbiol Biotechnol,2006,71(4):394-406.
[33]VUYST L.Nutritional factors affecting nisin production by Lactococcus lactis subsp.lactis NIZO 22186 in a synthetic medium[J].J Appl Bacteriol,1995,78(1):28-33.
[34]VAZQUEAZ J A,CABO M L,GONZALEZ M P,et al.The role of amino acids in nisin and pediocin production by two lactic acid bacteria:a factorial study[J].Enzyme Microb Technol,2004,34(3):319-325.
[35]YI H,HAN X,YANG Y,et al.Effect of exogenous factors on bacteriocin production from Lactobacillus paracasei J23 by using a resting cell system[J].Int J Mol Sci,2013,14(12):24355-24365.
[36]廖福荣.甲硫氨酸在头孢菌素C生物合成中的作用[J].国外医药(抗生素分册),2000,21(6):255-257,265.
[37]胡春霞.氮,磷和色氨酸对醉马草内生真菌共生体生长及麦角生物碱含量的影响[D].兰州:兰州大学,2013.
[38]LI M,CHEN Z,ZHANG X,et al.Enhancement of avermectin and ivermectin production by overexpression of the maltose ATP-binding cassette transporter in Streptomyces avermitilis[J].Bioresource Technol,2010,101(23):9228-9235.
[39]YANG E,FAN L,YAN J,et al.Influence of culture media,p H and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria[J].AMB Express,2018,8(1):10.
[40]VUKOTIC G,MIRKOVIC N,JOVCIC B,et al.Proteinase Prt P impairs lactococcin Lcn B activity in Lactococcus lactis BGMN1-501:new insights into bacteriocin regulation[J].Front Microbiol,2015(6):1-8.
[2]ZHANG J,YANG Y,YANG H,et al.Purification and partial characterization of bacteriocin Lac-B23,a novel bacteriocin production by Lactobacillus plantarum J23,isolated from Chinese traditional fermented milk[J].Front Microbiol,2018,9(1):2165.
[3]张建志.代谢工程改造大肠杆菌、酪丁酸梭菌生产苯乳酸和正丁醇[D].北京:中国农业大学,2017.
[4]NI Z J,ZHANG X,LIU F,et al.Effect of co-overexpression of nisin key genes on nisin production improvement in Lactococcus lactis LS01[J].Probiot Antimicrob Protein,2017,9(2):204-212.
[5]GUO W L,ZHANG Y B,LU J H,et al.Optimization of fermentation medium for nisin production from Lactococcus lactis subsp.lactis using response surface methodology(RSM)combined with artificial neural network-genetic algorithm(ANN-GA)[J].Afr J Biotechnol,2013,38(9):6264-6272.
[6]DEVI S M,HALAMI P M.Genetic variation of pln loci among probiotic Lactobacillus plantarum group strains with antioxidant and cholesterollowering ability[J].Probiot Antimicrob Protein,2017,1(10):1-12.
[7]SINGH N P,TIWARI A,BANSAL A,et al.Genome level analysis of bacteriocins of lactic acid bacteria[J].Comput Biol Chem,2015,56(6):1-6.
[8]COTTER P D,HILL C,ROSS R P.Bacteriocins:developing innate immunity for food[J].Nat Rev Microbiol,2005,3(10):777-788.
[9]JOHNSEN L,FIMLAND G,NISSEN-MEYER J.The C-terminal domain of pediocin-like antimicrobial peptides(class IIa bacteriocins)is involved in specific recognition of the C-terminal part of cognate immunity proteins and in determining the antimicrobial spectrum[J].J Biol Chem,2005,280(10):9243-9250.
[10]UTENG M,HAUGE H H,MARKWICK P R L,et al.Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide sakacin P and a sakacin P variant that is structurally stabilized by an inserted C-terminal disulfide bridge[J].Biochemistry,2003,42(39):11417-11426.
[11]SPRULE T,KAWULKA K E,GIBBS A C,et al.NMR solution structure of the precursor for carnobacteriocin B2,an antimicrobial peptide from Carnobacterium piscicola[J].Eur J Biochem,2004,271(9):1748-1756.
[12]HAUGEN H S,FUMLAND G,NISSEN-MEYER J,et al.Three-dimensional structure in lipid micelles of the pediocin-like antimicrobial peptide curvacin A[J].Biochemistry,2005,44(49):16149-16157.
[13]SIMON L,FREMAUX C,CENATIEMPO Y,et al.Sakacin G,a new type of antilisterial bacteriocin[J].Appl Environm Microbiol,2002,68(12):6416-6420.
[14]KAUR K,ANDREW L C,WISHART D S,et al.Dynamic relationships among type IIa bacteriocins:temperature effects on antimicrobial activity and on structure of the C-terminal amphipathicαhelix as a receptorbinding region[J].Biochemistry,2004,43(28):9009-9020.
[15]JIMENEZ-DIAZ R,RUIZ-BARBA J L,CATHCART D P,et al.Purification and partial amino acid sequence of plantaricin S,a bacteriocin produced by Lactobacillus plantarum LPCO10,the activity of which depends on the complementary action of two peptides[J].Appl Environm Microbiol,1995,61(12):4459-4463.
[16]MCCORMICK J K,POON A,SAILER M,et al.Genetic characterization and heterologous expression of brochocin-C,an antibotulinal,twopeptide bacteriocin produced by Brochothrix campestris ATCC 43754[J].Appl Environm Microbiol,1998,64(12):4757-4766.
[17]FIMLAND N,ROGNE P,FIMLAND G,et al.Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin plantaricin EF[J].BBA-Protein Proteom,2008,1784(11):1711-1719.
[18]ROGENE P,FIMLAND G,NISSEN-MEYE J,et al.Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin lactococcin G[J].BBA-Protein Proteom,2008,1784(3):543-554.
[19]ROGNE P,HAUGEN C,FIMLAND G,et al.Three-dimensional structure of the two-peptide bacteriocin plantaricin JK[J].Peptides,2009,30(9):1613-1621.
[20]ASPRI M,OCONNOR P M,FIELD D,et al.Application of bacteriocin-producing Enterococcus faecium isolated from donkey milk,in the bio-control of Listeria monocytogenes in fresh whey cheese[J].Int Dairy J,2017,73(10):1-9.
[21]VIGNOLO G,FADDA S,DE KAIRUZ M N,et al.Control of Listeria monocytogenes in ground beef by'Lactocin 705',a bacteriocin produced by Lactobacillus casei CRL 705[J].Int J Food Microbiol,1996,29(2):397-402.
[22]MARTIN-VISSCHER L A,GONG X,DUSZYK M,et al.The three-dimensional structure of carnocyclin A reveals that many circular bacteriocins share a common structural motif[J].J Biol Chem,2009,284(42):28674-28681.
[23]JIMENEZ M,BARRACHI-SACCILOTTO A C,VALDIVIA E,et al.Design,NMR characterization and activity of a 21-residue peptide fragment of bacteriocin AS-48 containing its putative membrane interacting region[J].J Pept Sci,2005,11(1):29-36.
[24]HENG N C K,WESCOMBE P A,BURTON J P,et al.The diversity of bacteriocins in Gram-positive bacteria[M].New York:Springer,2007:45-92.
[25]NISSEN-MEYER J,ROGENE P,OPPEGARD C,et al.Structure-function relationships of the non-lanthionine-containing peptide(class II)bacteriocins produced by gram-positive bacteria[J].Curr Pharmaceut Biotechnol,2009,10(1):19-37.
[26]吴清平,黄静敏,张菊梅,等.细菌素的合成与作用机制[J].微生物学通报,2010,37(10):1519-1524.
[27]QIU J,ZHUO Y,ZHU D,et al.Overexpression of the ABC transporter Avt AB increases avermectin production in Streptomyces avermitilis[J].Appl Microbiol Biot,2011,92(2):337-345.
[28]DRIDER D,FIMLAND G,HECHARD Y,et al.The continuing story of class IIa bacteriocins[J].Microbiol Molr Biol Rev,2006,70(2):564-582.
[29]VENEMA K,KOK J,MARUGG J D,et al.Functional analysis of the pediocin operon of Pediococcus acidilactici PAC1.0:Ped B is the immunity protein and Ped D is the precursor processing enzyme[J].Mol Microbiol,1995,17(3):515-522.
[30]HAVARSTEIN L S,DIEP D B,NES I F.A family of bacteriocin ABCtransporters carry out proteolytic processing of their substrates concomitant with export[J].Mol Microbiol,1995,16(2):229-240.
[31]PAL G,SRIVASTAVA S.In vitro activity of a recombinant ABC transporter protein in the processing of plantaricin E pre-peptide[J].Arch Microbiol,2015,197(6):1-7.
[32]SAVIJOKI K,INGMER H,VARMANEN P.Proteolytic systems of lactic acid bacteria[J].Appl Microbiol Biotechnol,2006,71(4):394-406.
[33]VUYST L.Nutritional factors affecting nisin production by Lactococcus lactis subsp.lactis NIZO 22186 in a synthetic medium[J].J Appl Bacteriol,1995,78(1):28-33.
[34]VAZQUEAZ J A,CABO M L,GONZALEZ M P,et al.The role of amino acids in nisin and pediocin production by two lactic acid bacteria:a factorial study[J].Enzyme Microb Technol,2004,34(3):319-325.
[35]YI H,HAN X,YANG Y,et al.Effect of exogenous factors on bacteriocin production from Lactobacillus paracasei J23 by using a resting cell system[J].Int J Mol Sci,2013,14(12):24355-24365.
[36]廖福荣.甲硫氨酸在头孢菌素C生物合成中的作用[J].国外医药(抗生素分册),2000,21(6):255-257,265.
[37]胡春霞.氮,磷和色氨酸对醉马草内生真菌共生体生长及麦角生物碱含量的影响[D].兰州:兰州大学,2013.
[38]LI M,CHEN Z,ZHANG X,et al.Enhancement of avermectin and ivermectin production by overexpression of the maltose ATP-binding cassette transporter in Streptomyces avermitilis[J].Bioresource Technol,2010,101(23):9228-9235.
[39]YANG E,FAN L,YAN J,et al.Influence of culture media,p H and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria[J].AMB Express,2018,8(1):10.
[40]VUKOTIC G,MIRKOVIC N,JOVCIC B,et al.Proteinase Prt P impairs lactococcin Lcn B activity in Lactococcus lactis BGMN1-501:new insights into bacteriocin regulation[J].Front Microbiol,2015(6):1-8.