微量醋酸亮丙瑞林对老龄雌性大鼠性腺轴的调节机制
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摘要
目的:观察注射微量醋酸亮丙瑞林对老龄雌性大鼠卵巢和血液雌激素水平的影响,探讨其对性腺轴的调节机制。方法:采用SD老龄雌性大鼠皮下注射不同剂量醋酸亮丙瑞林,应用HE染色、放免法观察卵巢、雌二醇(E2)、促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)和促黄体生成素(LH)水平的变化情况。结果:各给药组老龄雌鼠血中E2的水平高于老龄空白组(P<0.05),通过HE染色观察到各给药组大鼠卵泡数显著高于老龄空白组(P<0.05)。结论:微量醋酸亮丙瑞林能改善雌性大鼠卵巢的形态和功能,上调血液各个激素的水平,调控老龄性腺轴延缓卵巢的衰老。
Objective: To observe the effect of injecting microscale Leuprorelin Acetate on ovarian and blood estrogen levels in aged female rats, and to explore its regulation mechanism on the gonadal axis. Methods: Different doses of Leuprorelin Acetate were injected subcutaneously in aged SD female rats. Ovarian, the levels changed of estradiol(E2), gonadotropin-releasing hormone(GnRH),follicle-stimulating hormone(FSH) and luteinizing hormone(LH) were observed by HE stain and radioimmunoassay. Results: The levels of E2 in blood of aged female rats in the drug-administered group were higher than those in the old-aged control group(P<0.05).HE stain showed that the number of follicles in the treated group was significantly higher than that in the old-aged control group(P<0.05).Conclusion: The microscale Leuprorelin Acetate can improve the morphology and function of the ovaries in female rats, up-regulate the levels of various hormones in the blood, and can regulate aging of the gonads to delay aging.
Objective: To observe the effect of injecting microscale Leuprorelin Acetate on ovarian and blood estrogen levels in aged female rats, and to explore its regulation mechanism on the gonadal axis. Methods: Different doses of Leuprorelin Acetate were injected subcutaneously in aged SD female rats. Ovarian, the levels changed of estradiol(E2), gonadotropin-releasing hormone(GnRH),follicle-stimulating hormone(FSH) and luteinizing hormone(LH) were observed by HE stain and radioimmunoassay. Results: The levels of E2 in blood of aged female rats in the drug-administered group were higher than those in the old-aged control group(P<0.05).HE stain showed that the number of follicles in the treated group was significantly higher than that in the old-aged control group(P<0.05).Conclusion: The microscale Leuprorelin Acetate can improve the morphology and function of the ovaries in female rats, up-regulate the levels of various hormones in the blood, and can regulate aging of the gonads to delay aging.
引文
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[18]李妮,李花繁,李维玲,等.醋酸亮丙瑞林微球治疗子宫内膜异位症效果研究.中国性科学,2016,25(11):32-35
[19]徐昕红,于榕,贡泌燕,等.缓释醋酸亮丙瑞林微球对大鼠子宫异位内膜的抑制作用.中国临床药理学与治疗学,2004,9(8):876-879
[20]Meirow D,Assad G,Dor J,et al.The GnRH antagonist Cetrorelix reduce cyclosphamide-induced ovarian follicular destruction in mice.Hum Report,2004,19(6):1294-1299
[21]卢安清.亮丙瑞林治疗围绝经期功血子宫肌瘤的临床疗效分析.中外医疗,2016,35(22):131-132
[22]赵霞,方向明.醋酸亮丙瑞林预处理联合腹腔镜下子宫肌瘤剔除术治疗巨大子宫肌瘤临床观察.中华全科医学,2015,13(8):1293-1295
[23]丁曙霞,吴静,张建萍,等.醋酸亮丙瑞林治疗儿童中枢性性早熟的临床分析.中国现代医生,2014,52(16):47-50
[24]李恩.中国中西医结合临床全书.北京:中医古籍出版社,1996:1082
[25]范朗娣.卵巢临床与病理.天津:天津科学技术出版社,1993:16
[26]张镜如.生理学.4版.北京:人民卫生出版社,1999:414-417
[27]Dongliang Pan,Liming Dong,Lianchao Jin,et al.Serum testosterone suppression and potential for agonistic stimulation during chronic treatment with monthly depot formulation of domestic substitute of leuprorelin acetate microspheres for metastatic prostate cancer.The Chinese-German Journal of Clinical Oncology,2014(7):320-322
[28]张玲玲,应小燕.腹腔镜手术联合促性腺激素释放激素激动剂治疗子宫内膜异位症疗效的比较研究.中国微创外科杂志,2015,15(4):305-308
[29]金夏,周虎,桂云,等.醋酸亮丙瑞林缓释微球治疗子宫内膜异位症的疗效.中国妇幼保健,2012,27(19):2917-2920
[2]张晓芳,赵旭,李秀丽,等.不同绝经时期雌激素对女性高血压患者的心血管保护效应分析.中国动脉硬化杂志,2017,25(2):175-180
[3]邵秉一,于洋,杨德琴,等.雌激素缺乏导致的骨质疏松发病过程中BMMSC的Fas表达增强后受破骨细胞凋亡的影响.免疫学杂志,2016,32(10):853-856
[4]林雪完,王娟.雌激素和骨标志物与绝经后妇女骨质疏松的关系.中国老年学杂志,2017,37(2):378-379
[5]苏婷,赵元辰,宋殿荣.外阴阴道萎缩相关的围绝经期及绝经后女性性功能障碍.国际生殖健康/计划生育杂志,2017,36(6):514-518
[6]方旭红,毛菊芳.绝经后妇女应用雌激素对早老性痴呆病的影响.中国实用妇科与产科杂志,1999(2):51-52
[7]马敏,李斌.雌激素对阿尔茨海默病神经保护的作用机制.中国实用妇科与产科杂志,2014,30(4):316-320
[8]Beyer C.Estrogen and the developing brain.Anat Embryo,1999,199(5):379-390
[9]Green P S,Simpkins J W.Neuroprotective effects of estrogens potential echanisms of action.Int J Dev Neurosc,2000,18(4-5):347-358
[10]Hallbeck M,Hermanson O,Blomquist A.Distribution of preprovasopressin mRNA in the rat central nervous system.J Com Neurol,1999,411(2):181-200
[11]张庆红,胡玉珍,陈亚琼,等.大鼠垂体前叶中一些内分泌物质的共存.解剖学报,2001,32(4):350-351
[12]步世忠,孙梅,俞瑾,等.雌二醇对老年雌性大鼠下丘脑雌激素受体表达和β-内啡肽的影响.中国神经科学杂志,1998(2):81-84
[13]段立晖,周国庆,夏树开,等.雌激素对阿尔茨海默病大鼠学习记忆及海马神经元损伤保护作用的研究.医学研究生学报,2008,21(10):1040-1043
[14]雷德亮,罗学港,Peter R Mouton,等.雌激素对老年雌性小鼠齿状回和海马CA1区神经胶质细胞的影响-形态学观察和无偏性体视学定量分析.神经解剖学杂志,2004,20(4):337-342
[15]Wise P M,Dubal D B,Wilson M E,et al.Estrogens trophic and protective factors in the adult brain.Front Neuroendocrinol,2001,22:33-66
[16]Toung T J,Traystman R J,Hurn P D.Estrogen-mediated neuroprotection after experimental stroke in male rats.Stroke,1998,29:1666-1670
[17]陈明,李尧,房晓,等.国产亮丙瑞林与手术去势治疗前列腺癌的疗效比较.上海医药,2014,35(17):17-19
[18]李妮,李花繁,李维玲,等.醋酸亮丙瑞林微球治疗子宫内膜异位症效果研究.中国性科学,2016,25(11):32-35
[19]徐昕红,于榕,贡泌燕,等.缓释醋酸亮丙瑞林微球对大鼠子宫异位内膜的抑制作用.中国临床药理学与治疗学,2004,9(8):876-879
[20]Meirow D,Assad G,Dor J,et al.The GnRH antagonist Cetrorelix reduce cyclosphamide-induced ovarian follicular destruction in mice.Hum Report,2004,19(6):1294-1299
[21]卢安清.亮丙瑞林治疗围绝经期功血子宫肌瘤的临床疗效分析.中外医疗,2016,35(22):131-132
[22]赵霞,方向明.醋酸亮丙瑞林预处理联合腹腔镜下子宫肌瘤剔除术治疗巨大子宫肌瘤临床观察.中华全科医学,2015,13(8):1293-1295
[23]丁曙霞,吴静,张建萍,等.醋酸亮丙瑞林治疗儿童中枢性性早熟的临床分析.中国现代医生,2014,52(16):47-50
[24]李恩.中国中西医结合临床全书.北京:中医古籍出版社,1996:1082
[25]范朗娣.卵巢临床与病理.天津:天津科学技术出版社,1993:16
[26]张镜如.生理学.4版.北京:人民卫生出版社,1999:414-417
[27]Dongliang Pan,Liming Dong,Lianchao Jin,et al.Serum testosterone suppression and potential for agonistic stimulation during chronic treatment with monthly depot formulation of domestic substitute of leuprorelin acetate microspheres for metastatic prostate cancer.The Chinese-German Journal of Clinical Oncology,2014(7):320-322
[28]张玲玲,应小燕.腹腔镜手术联合促性腺激素释放激素激动剂治疗子宫内膜异位症疗效的比较研究.中国微创外科杂志,2015,15(4):305-308
[29]金夏,周虎,桂云,等.醋酸亮丙瑞林缓释微球治疗子宫内膜异位症的疗效.中国妇幼保健,2012,27(19):2917-2920