防风色原酮类成分的近红外光谱定量分析
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摘要
目的:本文建立了能快速测定防风中升麻素、4种色原酮含量之和的近红外光谱定量模型。方法:用高效液相色谱法测定55批防风饮片的4种色原酮的含量作为参考值,结合偏最小二乘回归法(PLS)建立回归模型。筛选了不同的光谱预处理方法、波段及最佳因子数,最终模型通过相关系数(R2)、预测均方根误差(RMSEP)和校正均方差(RMSEC)来评估其稳健性。结果:升麻素的最佳RMSEP值、RMSEC值、R2分别为0. 025 0、0. 024 0、0. 949 9,4种色原酮含量总和的最佳RMSEP值、RMSEC值、R2分别为0. 2460、0. 1720、0. 9110。结论:本研究建立的近红外光谱定量模型较准确,可用于对防风质量的快速检测。
Objective: To rapidly establish the detection method for cimifugin and the amounts of four chromones in Saposhnikoviae Radix by near infrared spectroscopy. Methods: The contents of four chromones of 55 batches of Saposhnikoviae Radix was determined by high performance liquid chromatography as reference value. Combined with partial least squares regression( PLS),the regression model was established. Different spectral pretreatment methods,wave ranges and optimal factors were selected. The stability of the models were evaluated by R2,RMSEP and RMSEC. Results: The optimum RMSEP values,RMSEC values and R2 values of cimifugin were 0. 025 0,0. 024 0 and 0. 949 9,respectively. The optimum RMSEP values,RMSEC values and R2 values of the amounts of four chromones were 0. 2460,0. 1720 and 0. 9110,respectively. Conclusion: The NIR quantitative models established in this study are accurate and can be used for rapid detection of quality of Saposhnikoviae Radix.
Objective: To rapidly establish the detection method for cimifugin and the amounts of four chromones in Saposhnikoviae Radix by near infrared spectroscopy. Methods: The contents of four chromones of 55 batches of Saposhnikoviae Radix was determined by high performance liquid chromatography as reference value. Combined with partial least squares regression( PLS),the regression model was established. Different spectral pretreatment methods,wave ranges and optimal factors were selected. The stability of the models were evaluated by R2,RMSEP and RMSEC. Results: The optimum RMSEP values,RMSEC values and R2 values of cimifugin were 0. 025 0,0. 024 0 and 0. 949 9,respectively. The optimum RMSEP values,RMSEC values and R2 values of the amounts of four chromones were 0. 2460,0. 1720 and 0. 9110,respectively. Conclusion: The NIR quantitative models established in this study are accurate and can be used for rapid detection of quality of Saposhnikoviae Radix.
引文
[1]国家药典委员会.中华人民共和国药典[S]. 1部.北京:中国医药科技出版社,2015:149.
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[3]刘双利,张春红,张连学.不同产地防风中4种色原酮成分比较研究[J].中草药,2007,38(5):776-778.
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[5]袁而文,严新龙,赵东辉.烟梗原料4种常规化学组分的近红外快速分析[J].红外技术,2015,37(1):82-86.
[6]杨阳,代涛,汪学楷.胆酸含量的近红外分析数学模型[J].化学研究与应用,2011,23(2):204-207.
[2]薛宝云,李文,李丽,等.防风色原酮甙类成分的药理活性研究[J].中国中药杂志,2000,25(5):297-299.
[3]刘双利,张春红,张连学.不同产地防风中4种色原酮成分比较研究[J].中草药,2007,38(5):776-778.
[4]戴锦娜.防风药效物质基础和相关成分药代动力学研究[D].沈阳:沈阳药科大学,2009.
[5]袁而文,严新龙,赵东辉.烟梗原料4种常规化学组分的近红外快速分析[J].红外技术,2015,37(1):82-86.
[6]杨阳,代涛,汪学楷.胆酸含量的近红外分析数学模型[J].化学研究与应用,2011,23(2):204-207.