改进型气升式反应器中白腐菌降解碱木素
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摘要
设计了一种白腐菌糙皮侧耳高效降解废水中碱木素的改进型气升式反应器,研究了内套管的开孔高度与脱色率的关系,并采用分批进料方式考察了改进后反应器中白腐菌对碱木素的降解效果。实验结果表明:反应器内套管的最佳开孔高度为45 cm;改进后反应器的漆酶酶活、COD去除率、脱色率分别最高达到了89.33 U/L、87.3%和81.39%,较改进前分别相对提高了17.5%、43.3%和39.6%。
A modi?ed airlift reactor was designed for degradation of alkali lignin in wastewater by white rot fungus Pleurotus ostreatus. The relationship between the opening height of inner casing and the decolorization rate was studied.The effect of white rot fungus on alkali lignin degradation in the improved reactor was studied by batch feeding. The experimental results showed that the optimum opening height of the casing in the reactor was 45 cm,and the highest laccase activity,COD removal rate and decolorization rate of the improved reactor were 89.33 U/L,87.3% and 81.39%respectively,which were 17.5%,43.3% and 39.6% higher than before.
A modi?ed airlift reactor was designed for degradation of alkali lignin in wastewater by white rot fungus Pleurotus ostreatus. The relationship between the opening height of inner casing and the decolorization rate was studied.The effect of white rot fungus on alkali lignin degradation in the improved reactor was studied by batch feeding. The experimental results showed that the optimum opening height of the casing in the reactor was 45 cm,and the highest laccase activity,COD removal rate and decolorization rate of the improved reactor were 89.33 U/L,87.3% and 81.39%respectively,which were 17.5%,43.3% and 39.6% higher than before.
引文
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[2]HUBER G W,IBORRA S,CORMA A.Synthesis of transportation fuels from biomass:chemistry,catalysts and engineering[J].Chem Rev,2006,106(9):4044-4098.
[3]AMIDON T E,LIU S.Water-based woody biorefinery[J].Biotechnol Adv,2009,27(5):542-550.
[4]崔玉虎,王奇,苟光俊,等.木质素催化降解液化的研究进展[J].材料导报,2017,31(5):112-116.
[5]侯红漫.白腐菌Pleurotus ostreatus漆酶及对蒽醌染料和碱木素脱色的研究[D].大连:大连理工大学,2003.
[6]王佳玲,余惠生,付时雨,等.白腐菌漆酶的研究进展[J].微生物学通报,1998,25(4):233-235.
[7]邓寒梅,邵可,梁家豪,等.漆酶的来源及固定化漆酶载体研究进展[J].生物技术通报,2017,33(6):10-15.
[8]曾涛,陈汉清,曾会才.木质素酶及其生产菌的筛选育种[J].基因组学与应用生物学,2009,28(3):578-582.
[9]赵丽红,周集体,孙洪军,等.白腐真菌反应器处理废水的研究进展[J].化工环保,2007,27(2):139-143.
[10]徐春燕,池春榕,刘祖文,等.气升式反应器内流动与传质研究进展[J].有色金属科学与工程,2016,7(5):110-118.
[11]孔黎明,谷和平,吕效平.超声波内环流气升式反应器去除水中苯酚的研究[J].化工环保,2004,24(6):462-466.
[12]赵东胜,刘桂敏,赵艳丽,等.气升式反应器研究进展[J].化工进展,2007,26(6):810-813.
[13]熊艳,魏宏斌,陈良才.气升式环流反应器在水处理中的应用及研究进展[J].中国给水排水,2010,26(10):9-12,18.
[14]PI K W,HUANG L,LI Z,et al.Oxygen mass transfer characteristics in an internal-loop airlift reactor with preset trumpet-shaped riser[J].Asia-Pacific J Chem Eng,2014,9(6):834-844.
[15]刘华冰,张帅,原平方.气升式环流反应器数值模拟方法选择及导流筒高度影响[J].精细与专用化学品,2017,25(4):33-41.
[16]高闯,王继锋.高长径比气升式环流反应器的流体动力学模型[J].沈阳化工大学学报,2016,30(3):217-222.
[17]WEN J P,PAN L H W,DU L P,et al.The denitrification of nitrate contained wastewater in a gas-liquid-solid three-phase flow airlift loop bioreactor[J].Biochem Eng J,2003,15(2):153-157.
[18]毛国柱,闻建平,贾晓强,等.应用多导流筒气升式环流生物反应器处理化肥工业含氨废水[J].环境工程,2006,24(2):7-8.
[19]WOLFENDEN B S,WILLSON R L.Radical-cations as reference chromogens in kinetic studies of ono-electron transfer reactions:pulse radiolysis studies of 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)[J].J Chem Soc,Perkin Trans 2,1982,1982(7):805-812.
[20]鲁光四,周怀东,李怡庭.水质分析方法[M].上海:学术书刊出版社,1991:198-204.