白兰花挥发油化学成分及其抑菌拒食活性研究
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摘要
利用水蒸气蒸馏法提取白兰花中的挥发性成分,通过气相色谱质谱联用技术(GC-MS)并结合计算机分析鉴定提取物中挥发油芳香成分,共鉴定出76个化合物,其中有37个萜类化合物、17个醇类化合物、6个烷烃类化合物和其它类型化合物。用面积归一化法测定挥发油中各种成分的相对含量,合计占总峰面积的93. 292%。同时对不同菌株进行抑菌实验,并探究不同浓度挥发油对粘虫的拒食作用。实验结果表明:用水蒸气蒸馏提取白兰花挥发油时,提取率为4. 18%,其最主要成分为芳樟醇;该方法提取的白兰花挥发油对水稻黄单胞菌和枯草芽孢杆菌抑制作用最好,并且对粘虫幼虫有很好拒食活性。
Volatile oils were obtained from the flowers of Michelia alba DC. by means of steam distillation technique. The volatile components in the extracts were analyzed by GC-MS and their relative contents were identified by peak area normalization method. The results showed that there are 76 identified compounds which include 37 terpenoids,17 alcohols,6 alkane compounds and other substances in the essential oils. The relative contents of all detected compounds in the essential oils are 93. 026%. In addition,we conducted antimicrobial experiments with different strains,and explored the antifeedant properties of essential oils in different concentrations against the worm Mythimna separata. The experimental results showed that the extraction rate was 4. 18% by using steam distillation extraction method. The most abundant component of the extracted oil is linalool. The essential oils showed most potent inhibitory activity against Xanthomonas oryzae and Bacillus subtilis among the tested strains and it had a good inhibitory effect on the armyworm Mythimna separata.
Volatile oils were obtained from the flowers of Michelia alba DC. by means of steam distillation technique. The volatile components in the extracts were analyzed by GC-MS and their relative contents were identified by peak area normalization method. The results showed that there are 76 identified compounds which include 37 terpenoids,17 alcohols,6 alkane compounds and other substances in the essential oils. The relative contents of all detected compounds in the essential oils are 93. 026%. In addition,we conducted antimicrobial experiments with different strains,and explored the antifeedant properties of essential oils in different concentrations against the worm Mythimna separata. The experimental results showed that the extraction rate was 4. 18% by using steam distillation extraction method. The most abundant component of the extracted oil is linalool. The essential oils showed most potent inhibitory activity against Xanthomonas oryzae and Bacillus subtilis among the tested strains and it had a good inhibitory effect on the armyworm Mythimna separata.
引文
1 Zhu FL(朱亮锋),Lu BY(陆碧瑶),Xu D(徐丹). A preliminary study on chemical constituents of the essential oil from Michelia alba DC.[J]. J Integr Plant Biol(植物学杂志),1982,4:355-359.
2 Pan LJ(潘利君),Wang JJ(汪涓涓),Huang YF(黄云峰),et al. Study on analysis of michelia flower&michelia leaf oil by GC/MS and their application in fragrances[J]. Flavour Fragr Cosmet(香料香精化妆品),2013,2:1-6.
3 Jiangsu New Medical College(江苏新医学院). Dictionary of Chinese medicine(中药大辞典)[M]. Shanghai:Shanghai People’s Publishing House,1977:703.
4 Li JJ(李军集),Li GQ(黎贵卿),Meng ZL(孟忠磊).Chemical constituents of volatile oils from the flowers and leaves of Michelia alba DC. in guangxi by GC-MS[J]. J Southwest Forestry Univ(西南林业大学学报),2012,32:102-106.
5 Yi XM(衣晓明),Song SQ(宋述芹),Gu M(谷茂). Volatile constituents of Michelia alba DC. analysis by GC-MS[J]. J Shenzhen Polytechnic(深圳职业技术学院学报),2016,15:50-53.
6 Gu FL(谷风林),Fang YM(房一明),Hu RS(胡荣锁),et al. Chemical constituents of the volatiles of Michelia alba DC. by GC-MS[J]. Chin J Trop Crops(热带作物学报),2011,32:1769-1773.
7 Hu RS(胡荣锁),Lu SF(卢少芳),Gu FL(谷风林),et al.Magnolia flavor components analysis of seven stages on blossom process base on the weak polar solvent extraction[J].Chin J Trop Crops(热带作物学报),2015,36:1707-1713.
8 Shang CQ,Hu YM,Deng CH,et al. Rapid determination of volatile constituents of Michelia alba flowers by gas chromatography-mass spectrometry with solid-phase microextraction[J]. J Chromatogr A,2002,942:283-288.
9 Wang HM,Lo WL,Huang LY,et al. Chemical constituents from the leaves of Michelia alba[J]. Nat Prod Res,2010,24:398-406.
10 Lee CH,Chen HL,Li HT,et al. Review on pharmacological activities of Michelia alba[J]. Int J Pharm&Ther,2014,5:289-292.
11 Li WZ(李为争),Hu JJ(胡晶晶),Yuan GH(原国辉),et al. Effect of piperine-and sanshool-experiences on larval feeding of Helicoverpa armigera[J]. Sci Agric Sin(中国农业科学),2015,48:2076-2084.
12 Xu YC(徐延驰). Occurrence reasons of maize army worm and its preventive measures[J]. Agric Sci Tech&eqpt(农业科技与装备),2016,259:21-22.
13 Jiang XF(江幸福),Luo LZ(罗礼智),Zhang L(张蕾),et al. Current status and trends in research on the oriental armyworm,Mythimna separate(Walker)in China[J]. Chin J Appl Entomol(应用昆虫学报),2014,51:881-889.
14 Gao SQ(高淑芹). The biological characteristics and prevention of Mythimna separate(Walker)[J]. J Mudanjiang Teachers Coll:Nat Sci(牡丹江师范学院学报:自科版),2002,2:25.
15 Feng GM(冯国明). The damage and control of Mythimna separate(Walker)[J]. CY Agri Farmers(农村.农业.农民),2013,5B:59.
16 Chen ZD(陈振东),Li LF(黎柳锋),Zeng XR(曾宪儒),et al. Antifeedant activity of chinese medicine Brucea javanica extracts against Plutella xylosetlla[J]. J Anhui Agric Sci(安徽农业科学),2015,43:122-123.
17 Yu ZY(余志银),Yu MM(喻明明),Luo JY(罗剑英),et al. Isolation,identification and antimicrobial activity of secondary metabolites from a soil-derived streptomyces from arid habitats of Qinghai[J]. Nat Prod Res Dev(天然产物研究与开发),2015,27:1900-1904.
18 Yang CW(杨超伟),Hu WH(胡伟华),Xia X(夏莘),et al. Study on anti-feeding active constituent of camphor leaves to Pieris rapae[J]. Nat Prod Res Dev(天然产物研究与开发),2014,26:42-45.
19 Huang XZ(黄相中),Yin Y(尹燕),Liu XF(刘晓芳),et al. Studies on chemical constituents of volatile oils from the flowers and leaves of Michelia alba DC. in Yunnan[J]. Chem Ind Forest Prod(林产化学与工业),2009,29:119-123.
20 Huang XZ(黄相中),Huang R(黄荣),Yin Y(尹燕),et al.Study on chemical constituents of essential oils from leaves and stems of Michelia alba DC.[J]. Food Sci(食品科学),2009,30:241-244.
2 Pan LJ(潘利君),Wang JJ(汪涓涓),Huang YF(黄云峰),et al. Study on analysis of michelia flower&michelia leaf oil by GC/MS and their application in fragrances[J]. Flavour Fragr Cosmet(香料香精化妆品),2013,2:1-6.
3 Jiangsu New Medical College(江苏新医学院). Dictionary of Chinese medicine(中药大辞典)[M]. Shanghai:Shanghai People’s Publishing House,1977:703.
4 Li JJ(李军集),Li GQ(黎贵卿),Meng ZL(孟忠磊).Chemical constituents of volatile oils from the flowers and leaves of Michelia alba DC. in guangxi by GC-MS[J]. J Southwest Forestry Univ(西南林业大学学报),2012,32:102-106.
5 Yi XM(衣晓明),Song SQ(宋述芹),Gu M(谷茂). Volatile constituents of Michelia alba DC. analysis by GC-MS[J]. J Shenzhen Polytechnic(深圳职业技术学院学报),2016,15:50-53.
6 Gu FL(谷风林),Fang YM(房一明),Hu RS(胡荣锁),et al. Chemical constituents of the volatiles of Michelia alba DC. by GC-MS[J]. Chin J Trop Crops(热带作物学报),2011,32:1769-1773.
7 Hu RS(胡荣锁),Lu SF(卢少芳),Gu FL(谷风林),et al.Magnolia flavor components analysis of seven stages on blossom process base on the weak polar solvent extraction[J].Chin J Trop Crops(热带作物学报),2015,36:1707-1713.
8 Shang CQ,Hu YM,Deng CH,et al. Rapid determination of volatile constituents of Michelia alba flowers by gas chromatography-mass spectrometry with solid-phase microextraction[J]. J Chromatogr A,2002,942:283-288.
9 Wang HM,Lo WL,Huang LY,et al. Chemical constituents from the leaves of Michelia alba[J]. Nat Prod Res,2010,24:398-406.
10 Lee CH,Chen HL,Li HT,et al. Review on pharmacological activities of Michelia alba[J]. Int J Pharm&Ther,2014,5:289-292.
11 Li WZ(李为争),Hu JJ(胡晶晶),Yuan GH(原国辉),et al. Effect of piperine-and sanshool-experiences on larval feeding of Helicoverpa armigera[J]. Sci Agric Sin(中国农业科学),2015,48:2076-2084.
12 Xu YC(徐延驰). Occurrence reasons of maize army worm and its preventive measures[J]. Agric Sci Tech&eqpt(农业科技与装备),2016,259:21-22.
13 Jiang XF(江幸福),Luo LZ(罗礼智),Zhang L(张蕾),et al. Current status and trends in research on the oriental armyworm,Mythimna separate(Walker)in China[J]. Chin J Appl Entomol(应用昆虫学报),2014,51:881-889.
14 Gao SQ(高淑芹). The biological characteristics and prevention of Mythimna separate(Walker)[J]. J Mudanjiang Teachers Coll:Nat Sci(牡丹江师范学院学报:自科版),2002,2:25.
15 Feng GM(冯国明). The damage and control of Mythimna separate(Walker)[J]. CY Agri Farmers(农村.农业.农民),2013,5B:59.
16 Chen ZD(陈振东),Li LF(黎柳锋),Zeng XR(曾宪儒),et al. Antifeedant activity of chinese medicine Brucea javanica extracts against Plutella xylosetlla[J]. J Anhui Agric Sci(安徽农业科学),2015,43:122-123.
17 Yu ZY(余志银),Yu MM(喻明明),Luo JY(罗剑英),et al. Isolation,identification and antimicrobial activity of secondary metabolites from a soil-derived streptomyces from arid habitats of Qinghai[J]. Nat Prod Res Dev(天然产物研究与开发),2015,27:1900-1904.
18 Yang CW(杨超伟),Hu WH(胡伟华),Xia X(夏莘),et al. Study on anti-feeding active constituent of camphor leaves to Pieris rapae[J]. Nat Prod Res Dev(天然产物研究与开发),2014,26:42-45.
19 Huang XZ(黄相中),Yin Y(尹燕),Liu XF(刘晓芳),et al. Studies on chemical constituents of volatile oils from the flowers and leaves of Michelia alba DC. in Yunnan[J]. Chem Ind Forest Prod(林产化学与工业),2009,29:119-123.
20 Huang XZ(黄相中),Huang R(黄荣),Yin Y(尹燕),et al.Study on chemical constituents of essential oils from leaves and stems of Michelia alba DC.[J]. Food Sci(食品科学),2009,30:241-244.