甲氧虫酰肼与3种药剂对甜菜夜蛾的联合毒力及亚致死效应
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摘要
为筛选出甲氧虫酰肼与其他药剂对甜菜夜蛾具有增效作用的混配组合,采用表面涂抹法测定甲氧虫酰肼与虱螨脲、氯虫苯甲酰胺和高效氯氰菊酯混配对甜菜夜蛾3龄幼虫的联合毒力,并在此基础上研究了甲氧虫酰肼与虱螨脲混用对甜菜夜蛾的杀卵活性及亚致死效应。结果表明,甲氧虫酰肼、虱螨脲、氯虫苯甲酰胺和高效氯氰菊酯对甜菜夜蛾的LD50分别为38.20ng·cm~(-2)、44.66 ng·cm~(-2)、17.54 ng·cm~(-2)、4.13 ng·cm~(-2),甲氧虫酰肼与虱螨脲复配表现出明显的增效作用,配比(质量浓度比)为6∶7时增效作用最大,共毒因子高达135.4,而甲氧虫酰肼与氯虫苯甲酰胺和氯氟氰菊酯混用均表现出拮抗作用;甲氧虫酰肼与虱螨脲复配(配比6∶7)质量浓度为10 mg·L-1和100 mg·L-1时,卵孵化率和幼虫存活率分别为56.11%、36.53%和48.08%、17.70%;亚致死剂量的甲氧虫酰肼与虱螨脲混剂与单剂相比显著延长幼虫发育历期,抑制幼虫的体质量和蛹质量,降低化蛹率和单雌产卵量。因此,甲氧虫酰肼和虱螨脲混用可以显著提高对甜菜夜蛾杀虫活性,抑制其种群增长,同时兼有较强的杀卵作用。
引文
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[19]吴钜文.昆虫生长调节剂在农业害虫防治中的应用[J].农药,2002,41(4):6-8.
[2]贾变桃,沈晋良.甜菜夜蛾对几类新型杀虫剂的早期抗性监测[J].棉花学报,2008,20(5):359-363.
[3]苏宏华,范娜,李达,等.转Bt基因棉对甜菜夜蛾的影响[J].植物保护,2010,36(5):1-5.
[4]黄东林,夏锦瑜,柳慧,等.转CpTI+Bt基因棉和转Bt基因棉对甜菜夜蛾种群增长和行为的影响[J].植物保护学报,2007,34(5):461-465.
[5]司升云,周利琳,望勇,等.湖北省甜菜夜蛾田间种群抗药性监测[J].植物保护,2009,35(1):114-117.
[6]袁永达,王冬生,於文俊.上海地区甜菜夜蛾的抗药性及防治[J].上海农业学报,2006,22(1):42-45.
[7]侍甜.甜菜夜蛾抗性监测及甲维盐和高效氯氰菊酯抗性种群的抗性机理和抗性遗传方式[D].南京:南京农业大学,2011.
[8]戴瀚洋,孙洋,柏立新,等.亚致死浓度甲维盐胁迫对甜菜夜蛾幼虫解毒酶系活力及其相关基因表达量的影响[J].棉花学报,2015,27(2):149-158.
[9]陈吉祥,任相亮,姜伟丽,等.双酰肼类昆虫生长调节剂的作用机理及对甜菜夜蛾的防治现状简述[J].农药,2018,57(5):316-320.
[10]全林发,张怀江,孙丽娜,等.杀虫剂对害虫的亚致死效应研究进展[J].农学学报,2016,6(5):33-38.
[11]Pineda S,Schneider M I,Smagghe G,et al.Lethal and sublethal effects of methoxyfenozide and spinosad on Spodoptera littoralis(Lepidoptera:Noctuidae)[J].Journal of Economic Entomology,2007,100(3):773-780.
[12]Sundaram M,Palli S R,Smagghe G,et al.Effect of RH-5992 on adult development in the spruce budworm,Choristoneura fumiferana[J].Insect Biochemistry and Molecular Biology,2002,32(2):225-231.
[13]Seth R K,Kaur J J,Rao D K,et al.Effects of larval exposure to sublethal concentrations of the ecdysteroid agonists RH-5849 and tebufenozide(RH-5992)on male reproductive physiology in Spodoptera litura[J].Journal of Insect Physiology,2004,50(6):505-517.
[14]Pineda S,Martínez A M,Figueroa J I,et al.Influence of azadirachtin and methoxyfenozide on life parameters of Spodoptera littoralis(Lepidoptera:Noctuidae)[J].Journal of Economic Entomology,2009,102(4):1490-1496.
[15]Thamthiankul C S,Tantichodok A,Panbangred W.Spore stage expression of a vegetative insecticidal gene increase toxicity of Bacillus thuringiensis subsp.aizawai SP41 against Spodoptera exigua[J].Journal of Biotechnology,2008,136(3/4):122-128.
[16]Eldefrawi M E,Toppozada A,Mansour N,et al.Toxicological studies on the Egyptian cotton leafworm,Prodenia litura.I.Susceptibility of different larval instars of Prodenia to insecticides[J].Journal of Economic Entomology,1964,57(4):591-593.
[17]洪珊珊,贾变桃,李琴,等.6种杀虫剂对小菜蛾不同虫态室内活性研究[J].现代农药,2014,13(5):43-47.
[18]朱卫锋,任太军,曾鑫年.虱螨脲与虫酰肼的增效作用及防治斜纹夜蛾的药效[J].广东农业科学,2011(11):104-106.
[19]吴钜文.昆虫生长调节剂在农业害虫防治中的应用[J].农药,2002,41(4):6-8.