颈腰康胶囊的抗炎消肿药效物质基础研究
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摘要
目的:研究颈腰康胶囊抗炎消肿药效的物质基础,为该制剂的二次开发、质量控制方法建立和技术改造升级提供依据。方法:采用不同溶剂以及大孔吸附树脂法对颈腰康胶囊进行组分提取分离,获得A组分(总体富集部位)、B组分(氯仿萃取部位)、C组分(水层部位)和D组分(60%乙醇洗脱部位)。通过小鼠耳廓肿胀实验和大鼠足跖肿胀实验,以醋酸地塞米松为阳性对照药物,考察颈腰康胶囊及其不同提取部位(A、B、C、D组分)的抗炎消肿作用,筛选功效部位。采用超高效液相色谱-四极杆飞行时间质谱技术(UPLC-Q-TOF-MS)解析功效部位,鉴定其中所含化合物并进行组方药材归属。结果:B组分(氯仿萃取部位)+D组分(60%乙醇洗脱部位)对大鼠或小鼠的抗炎消肿疗效与颈腰康胶囊相当,提示二者发挥了协同抗炎消肿作用,是颈腰康胶囊的功效部位。UPLC-Q-TOF-MS检测及鉴定结果显示,B组分中含有士的宁、黄柏碱、杠柳苷元、石松四酮醇、11-羰基-β-乳香酸等13个化合物,分别归属于马钱子、防己、香加皮、伸筋草、乳香等药材;D组分中含有腺嘌呤、羟基红花黄色素A、黄柏碱、Neoeriocitrin、姜状三七皂苷R1等7个化合物,分别归属于地龙、红花、防己、骨碎补、牛膝等药材。结论:颈腰康胶囊具有显著的抗炎消肿作用,其氯仿萃取部位与60%乙醇洗脱部位可能共同为其抗炎消肿的功效部位,所含主要成分为生物碱类、黄酮类及乳香酸类化合物。
OBJECTIVE:To study the anti-inflammatory and detumescent pharmacodynamic material basis of Jingyaokangcapsule,and to provide reference for secondary development,the establishment of quality control method and technologicalupgrading of the preparations. METHODS:The constituents of Jingyaokang capsule were extracted and separated with differentsolvents and macroporous adsorption resin to obtain constituent A (overall enrichment part),constituent B (chloroform extractionpart),constituent C (water-course part)and constituent D (elution part of 60% ethanol). Using dexamethasone acetate as positivecontrol,the anti-inflammatory and detumescent effects of Jingyaokang capsule and different extraction parts (constituents A,B,C,D)were investigated by mice ear edema and rat paw edema tests to screen the active fraction. UPLC-Q-TOF-MS method was usedto analyze active constituent,identify compounds and attribute medicinal material. RESULTS:Anti-inflammatory and detumescenteffects of constituent B (chloroform extraction part)+ constituent D (elution part of 60% ethanol) were similar to those ofJingyaokang capsule in rats or mice,indicating both had synergistic anti-inflammatory and detumescent effects and were activeconstituents of Jingyaokang capsule. UPLC-Q-TOF-MS detection and identification showed that constituent B contained 13 compounds as strychnine,phellodendrine,periplogenin,tetraketone alcohol,11-carbonyl-β-mastic acid,attributing to Strychnosnux-vomica,Stephania tetrandra,Periploca sepium,Lycopodium japonicum,Boswellia carterii,etc. Constituent D contained 7 compounds as adenine, hydroxysafflower yellow A, phellodendrine, neoeriocitrin, zingibroside R1,attributing to rainworm,Carthamus tinctorius,Stephania tetrandra,Davallia mariesii,Achyranthes bidentata,etc. CONCLUSIONS:Jingyaokang capsuleshows the significant anti-inflammation and detumescent effects. The chloroform extraction part is synergistic with 60% ethanolelution part,which are the active constituents of anti-inflammation and detumescence,mainly including alkaloids,flavonoids andboswellic acids.
OBJECTIVE:To study the anti-inflammatory and detumescent pharmacodynamic material basis of Jingyaokangcapsule,and to provide reference for secondary development,the establishment of quality control method and technologicalupgrading of the preparations. METHODS:The constituents of Jingyaokang capsule were extracted and separated with differentsolvents and macroporous adsorption resin to obtain constituent A (overall enrichment part),constituent B (chloroform extractionpart),constituent C (water-course part)and constituent D (elution part of 60% ethanol). Using dexamethasone acetate as positivecontrol,the anti-inflammatory and detumescent effects of Jingyaokang capsule and different extraction parts (constituents A,B,C,D)were investigated by mice ear edema and rat paw edema tests to screen the active fraction. UPLC-Q-TOF-MS method was usedto analyze active constituent,identify compounds and attribute medicinal material. RESULTS:Anti-inflammatory and detumescenteffects of constituent B (chloroform extraction part)+ constituent D (elution part of 60% ethanol) were similar to those ofJingyaokang capsule in rats or mice,indicating both had synergistic anti-inflammatory and detumescent effects and were activeconstituents of Jingyaokang capsule. UPLC-Q-TOF-MS detection and identification showed that constituent B contained 13 compounds as strychnine,phellodendrine,periplogenin,tetraketone alcohol,11-carbonyl-β-mastic acid,attributing to Strychnosnux-vomica,Stephania tetrandra,Periploca sepium,Lycopodium japonicum,Boswellia carterii,etc. Constituent D contained 7 compounds as adenine, hydroxysafflower yellow A, phellodendrine, neoeriocitrin, zingibroside R1,attributing to rainworm,Carthamus tinctorius,Stephania tetrandra,Davallia mariesii,Achyranthes bidentata,etc. CONCLUSIONS:Jingyaokang capsuleshows the significant anti-inflammation and detumescent effects. The chloroform extraction part is synergistic with 60% ethanolelution part,which are the active constituents of anti-inflammation and detumescence,mainly including alkaloids,flavonoids andboswellic acids.
引文
[1]药智数据.颈腰康胶囊[EB/OL].[2018-11-27].https://db yaozh.com/instruct/101274.html.
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[13]祁艳,陈军,蔡宝昌,等.马钱子总生物碱提取纯化工艺及抗肿瘤研究[J].中成药,2010,32(3):405-408.
[14]蔡卓亚,周自桂,李萍,等.伸筋草化学成分及药理作用研究进展[J].中草药,2015,46(2):297-304.
[15]杨欣欣,包永睿,王帅,等.防己生物碱类成分提取纯化工艺研究[J].中成药,2014,36(6):1306-1309.
[16]扈晓佳,殷莎,袁婷婷,等.红花的化学成分及其药理活性研究进展[J].药学实践杂志,2013,31(3):161-168、197.
[17]彭双,韩立峰,王涛,等.骨碎补中的化学成分及药理作用研究进展[J].天津中医药大学学报,2012,31(2):122-125.
[18]王利萍,刘建利.香加皮的化学成分和药理作用研究进展[J].中草药,2009,40(3):493-496.
[2]徐叔云.药理实验方法学[M].3版.北京:人民卫生出版社,2002:202-204.
[3]吕鹏.跌打消肿止痛灵的药效学研究[D].南宁:广西医科大学,2012.
[4]谷捷,李鑫,余黄合,等.二甲苯致小鼠耳肿胀急性炎症模型的建立[J].湖南中医药大学学报,2016,36(5):32-35.
[5]魏春华,程虹毓,高燕萍,等.藏药矮紫堇解热镇痛抗炎作用的研究[J].中国新药杂志,2017,26(3):337-342.
[6]朱力阳,涂禾,马建,等.二黄凝胶贴膏镇痛抗炎作用及机制研究[J].中药药理与临床,2017,33(2):148-151.
[7]姜溪,陈芙蓉,任雷鸣,等.注射用复方骨肽的药效学及其作用机制研究[J].现代药物与临床,2017,32(6):961-966.
[8]徐杰,姜文月,王美慧,等.颈腰康胶囊镇痛药效物质基础研究[J].中药药理与临床,2018,34(5):112-115.
[9]沈舒,王琼,李友宾.牛膝的化学成分和药理作用研究进展[J].海峡药学,2011,23(11):1-6.
[10]常允平,韩英梅,张俊艳.乳香的化学成分和药理活性研究进展[J].现代药物与临床,2012,27(1):52-59.
[11]沈涛,娄红祥.没药的化学成分及其生物活性[J].天然产物研究与开发,2008,20(2):360-366.
[12]唐敏,伍冠一,朱婵,等.马钱子碱镇痛研究进展[J].中草药,2014,45(12):1791-1795.
[13]祁艳,陈军,蔡宝昌,等.马钱子总生物碱提取纯化工艺及抗肿瘤研究[J].中成药,2010,32(3):405-408.
[14]蔡卓亚,周自桂,李萍,等.伸筋草化学成分及药理作用研究进展[J].中草药,2015,46(2):297-304.
[15]杨欣欣,包永睿,王帅,等.防己生物碱类成分提取纯化工艺研究[J].中成药,2014,36(6):1306-1309.
[16]扈晓佳,殷莎,袁婷婷,等.红花的化学成分及其药理活性研究进展[J].药学实践杂志,2013,31(3):161-168、197.
[17]彭双,韩立峰,王涛,等.骨碎补中的化学成分及药理作用研究进展[J].天津中医药大学学报,2012,31(2):122-125.
[18]王利萍,刘建利.香加皮的化学成分和药理作用研究进展[J].中草药,2009,40(3):493-496.