葛根素对镉暴露致大鼠大脑皮质氧化损伤的保护效应
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摘要
为探究葛根素(Pur)在镉(Cd)致大鼠大脑皮质氧化损伤中的保护作用,40只雄性SD大鼠随机分为4组,分别为对照组、镉组、葛根素组、镉与葛根素共处理组。试验期间,所有大鼠均自由饮用纯净水,每天按200 mg/kg剂量的葛根素对葛根素组、镉与葛根素共处理组大鼠进行灌胃,同时以相同剂量的纯净水将对照组、镉组大鼠进行灌胃,连续处理5周,镉组、镉与葛根素共处理组大鼠在处理4周后连续一周每天用醋酸镉按2.5 mg/kg剂量进行腹腔注射。5周后,分离大鼠大脑皮质,测定大脑皮质内总抗氧化能力(T-AOC),超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)的含量。结果表明,与对照组相比,镉组大鼠大脑皮质T-AOC,SOD和GSH-Px活性显著降低(P<0.05),MDA含量显著升高(P<0.05);与镉组相比,镉与葛根素共处理组T-AOC,SOD和GSH-Px活性显著升高(P<0.05),MDA含量显著降低(P<0.05)。说明葛根素在镉致大鼠大脑皮质氧化损伤中具有一定的保护作用。
In order to evaluate the oxidative damage induced by cadmium exposure in cerebral cortex of SD rats and protective effects of puerarin,forty male SD rats(70±5) g were randomly divided into four groups:control group,Cd-treated group,puerarin group,Cd and puerarin co-treated group.All rats were free acess to purified water during the experiment.When puerarin(200 mg/kg) was given to rats by gavage in the puerarin group and the co-treated group,the same dose of purified water was given to rats by gavage concurrently in the control group and the Cd-treated group over 5 weeks.The Cd Ac2(2.5 mg/kg) was given to rats by intraperitoneal injection daily over 1 week at the fifth week in the Cd group and the co-treated group.Five weeks later,the cerebral cortex was isolated.The T-AOC,the activities of SOD and GSH-Px and the contents of MDA in cerebral cortex were measured.In result,compared with the control group,the T-AOC,the activities of SOD and GSH-Px in cadmium-exposed groups all decreased significantly(P<0.05),and the contents of MDA in cerebral cortex increased significantly(P<0.05).Compared with the cadmium group,the T-AOC,the activities of SOD and GSH-Px in co-treated groups all increased significantly(P<0.05),and the contents of MDA decreased significantly(P<0.05).The results indicated that puerarin can protect the oxidative damage caused by cadmium in cerebral cortex of rats.
In order to evaluate the oxidative damage induced by cadmium exposure in cerebral cortex of SD rats and protective effects of puerarin,forty male SD rats(70±5) g were randomly divided into four groups:control group,Cd-treated group,puerarin group,Cd and puerarin co-treated group.All rats were free acess to purified water during the experiment.When puerarin(200 mg/kg) was given to rats by gavage in the puerarin group and the co-treated group,the same dose of purified water was given to rats by gavage concurrently in the control group and the Cd-treated group over 5 weeks.The Cd Ac2(2.5 mg/kg) was given to rats by intraperitoneal injection daily over 1 week at the fifth week in the Cd group and the co-treated group.Five weeks later,the cerebral cortex was isolated.The T-AOC,the activities of SOD and GSH-Px and the contents of MDA in cerebral cortex were measured.In result,compared with the control group,the T-AOC,the activities of SOD and GSH-Px in cadmium-exposed groups all decreased significantly(P<0.05),and the contents of MDA in cerebral cortex increased significantly(P<0.05).Compared with the cadmium group,the T-AOC,the activities of SOD and GSH-Px in co-treated groups all increased significantly(P<0.05),and the contents of MDA decreased significantly(P<0.05).The results indicated that puerarin can protect the oxidative damage caused by cadmium in cerebral cortex of rats.
引文
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[14]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技导报,2013,15(5):53-58.
[15]王咏梅.自由基与谷胱甘肽过氧化物酶[J].解放军药学学报,2005,21(5):369-371.
[16]翟晓虎,杨海锋,陈慧英,等.丙二醛的毒性作用及检测技术研究进展[J].上海农业学报,2018,34(1):144-148.
[17]卞建春,邢华,王捍东,等.灌胃染镉对大鼠体内某些酶的影响及镉的器官分布[J].中国兽医学报,2002,22(06):592-594.
[18]陈洁,赵世文,杨金龙,等.亚慢性铅与镉单独或联合暴露致大鼠氧化损伤作用及α-硫辛酸的保护效应[J].中国兽医科学,2018,48(6):790-796.
[19]王东红,王春爱,薛建军.葛根素的研究进展[J].西部中医药,2017,30(1):139-142.
[20]黄雄峰,汪建民.葛根素的神经保护作用机制研究进展[J].中国实验方剂学杂志,2015,21(4):224-230.
[21]Chung M J,Sung N J,Park C S,et al.Antioxidative and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2cells and in C57 BL/6J mice[J].Eur J Pharmacol,2008,578(2):159-170.
[22]余雪源,仲英,左春旭,等.羟乙葛根素对大鼠局灶性脑缺血再灌注氧化损伤的保护作用[J].中国生化药物杂志,2012,33(1):4-7,11.
[23]黎荣,徐灵源,梁韬,等.葛根素对6-羟多巴胺致帕金森病模型大鼠黑质组织神经细胞的保护作用[J].中国实验方剂学杂志,2013,19(2):247-250.
[24]殷利春,黄晓明,何民,等.葛根素对实验性脑出血大鼠脑组织SOD、MDA水平变化和神经功能障碍的相关性研究[J].心脑血管病防治,2010,10(5):354-355,376.
[25]张蕊,曹焕军,高尔.葛根素对家兔脑缺血再灌注损伤的抗自由基作用[J].潍坊医学院学报,2002,24(4):267-269.
[2]龚频,白晓换,陈福欣,等.镉损伤小鼠肾脏形态学研究及姜黄素的保护作用[J].陕西科技大学学报,2015,33(3):140-143.
[3]许立芹,王东,蔡恒,等.镉致胎鼠骨骼发育异常的形态学观察[J].滨州医学院学报,2008,31(1):11-13,15.
[4]潘顺叶,范文桐,王光光,等.慢性饮水染镉对大鼠睾丸的病理学影响[J].黑龙江畜牧兽医,2015(19):47-49,53,295.
[5]袁燕.镉对大鼠大脑皮质神经细胞毒性损伤的机制[D].扬州:扬州大学,2012.
[6]Antonio M T,Corpas I,Leret M L.Neurochemical changes in newborn rat’s brain after gestational cadmium and lead exposure[J].Toxicol Lett,1999,104(1):1-9.
[7]臧贵勇,王景传,姜俸蓉,等.慢性镉中毒小鼠中脑黑质的形态学研究[J].中国公共卫生,2001,17(11):1015-1016.
[8]Jiang L F,Yao T M,Zhu Z L,et al.Impacts of Cd(Ⅱ)on the conformation and self-aggregation of Alzheimer's tau fragment corresponding to the third repeat of microtubule-binding domain[J].BBA,2007,1774(11):1414-1421.
[9]袁燕,刘学忠,卞建春,等.镉对大鼠大脑皮质神经细胞的氧化损伤及N-乙酰半胱氨酸的保护效应[J].中国兽医学报,2011,31(3):391-393,401.
[10]谭军,潘登,孙兆印.葛根素预处理对大鼠脑缺血再灌注损伤后脑组织中一氧化氮水平的影响[J].新乡医学院学报,2008,25(5):453-455.
[11]王立祥,曾季平,魏欣冰,等.葛根素对过氧化氢诱导PC12细胞损伤的保护作用[J].山东大学学报(医学版),2006,44(1):20-23.
[12]Viaene M K,Masschelein R,Leenders J,et al.Neurobehavioural effects of occupational exposure to cadmium:a cross sectional epidemiological study[J].Occup Environ Med,2000,57(1):19-27.
[13]胡一鸿,牛健康.超氧化物歧化酶研究进展[J].生物学教学,2005,30(1):2-4.
[14]董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技导报,2013,15(5):53-58.
[15]王咏梅.自由基与谷胱甘肽过氧化物酶[J].解放军药学学报,2005,21(5):369-371.
[16]翟晓虎,杨海锋,陈慧英,等.丙二醛的毒性作用及检测技术研究进展[J].上海农业学报,2018,34(1):144-148.
[17]卞建春,邢华,王捍东,等.灌胃染镉对大鼠体内某些酶的影响及镉的器官分布[J].中国兽医学报,2002,22(06):592-594.
[18]陈洁,赵世文,杨金龙,等.亚慢性铅与镉单独或联合暴露致大鼠氧化损伤作用及α-硫辛酸的保护效应[J].中国兽医科学,2018,48(6):790-796.
[19]王东红,王春爱,薛建军.葛根素的研究进展[J].西部中医药,2017,30(1):139-142.
[20]黄雄峰,汪建民.葛根素的神经保护作用机制研究进展[J].中国实验方剂学杂志,2015,21(4):224-230.
[21]Chung M J,Sung N J,Park C S,et al.Antioxidative and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2cells and in C57 BL/6J mice[J].Eur J Pharmacol,2008,578(2):159-170.
[22]余雪源,仲英,左春旭,等.羟乙葛根素对大鼠局灶性脑缺血再灌注氧化损伤的保护作用[J].中国生化药物杂志,2012,33(1):4-7,11.
[23]黎荣,徐灵源,梁韬,等.葛根素对6-羟多巴胺致帕金森病模型大鼠黑质组织神经细胞的保护作用[J].中国实验方剂学杂志,2013,19(2):247-250.
[24]殷利春,黄晓明,何民,等.葛根素对实验性脑出血大鼠脑组织SOD、MDA水平变化和神经功能障碍的相关性研究[J].心脑血管病防治,2010,10(5):354-355,376.
[25]张蕊,曹焕军,高尔.葛根素对家兔脑缺血再灌注损伤的抗自由基作用[J].潍坊医学院学报,2002,24(4):267-269.