不同产地玄参内生真菌种群结构的比较分析
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摘要
目的研究不同产地玄参内生真菌的种群结构差异。方法通过形态和分子手段相结合的方式对来自浙江磐安、陕西安康、湖北武穴、湖南邵东、四川达州和安徽亳州共6个产地的玄参进行内生真菌的分离、鉴定及种群结构分析。结果共分离得到3 052株内生真菌划分为153个形态型,经分子系统学分析,共划分为84个分类单元,分属于25个属,其中间座壳属、镰刀菌属、黑孢属、链格孢属、茎点霉属、棒孢属、附球菌属、枝孢属为6个产地内生真菌共有属,各产地的优势属不同。相似性分析表明,不同产地玄参内生真菌的组成结构上存在差异。Shannon-wiener多样性指数及Simpson指数分析表明浙江磐安产地玄参内生真菌多样性较高,四川达州产地的多样性稍低。结论系统研究玄参内生真菌的多样性与群落结构,阐明内生真菌在植物组织中的分布规律,可为玄参内生真菌的开发利用提供基础资料和科学依据。
Objective To analyze the distribution of endophytic fungi in Scrophularia ningpoensis from different habitats. Methods The endophytic fungi in S. ningpoensis from six localities(Pan'an of Zhejiang Province, Ankang of Shaanxi Province, Wuxue of Hubei Province, Shaodong of Hunan Province, Dazhou of Sichuan Province, and Bozhou of Anhui Province) were isolated,identified, and analyzed according to the morphology and ITS sequences. Results A total of 3 052 strains of endophytic fungi were isolated from S. ningpoensis in six localities. A total of 84 fungal taxa were identified according to the morphology and ITS sequences,these strains were belong to 25 genera. Thereinto Diaporthe, Fusarium, Nigrospora, Alternaria, Phoma, Corynespora, Epicoccum, and Cladosporium were common genera to S. ningpoensis from six localities, but the dominant genus was different: Fusarium was the dominant genus from Anhui, Hubei, Hunan, and Shaanxi; Alternaria was the dominant genus from Sichuan; Diaporthe was the dominant genus from Zhejiang. According to the similarity coefficient, the composition of the endophytic fungi was distinctly different between six localities. The Shannon-wiener diversity index and Simpson index of endophytic fungi in S. ningpoensis from Zhejiang were found higher than others. The diversity of endophytic fungi in S. ningpoensis from Sichuan was lower than others. Conclusion Systematic studying the diversity and community structure of endophytic fungi in S. ningpoensis and clarifying their distribution regularity in plant tissues can offer basic data and scientific basis for their development and utilization.
Objective To analyze the distribution of endophytic fungi in Scrophularia ningpoensis from different habitats. Methods The endophytic fungi in S. ningpoensis from six localities(Pan'an of Zhejiang Province, Ankang of Shaanxi Province, Wuxue of Hubei Province, Shaodong of Hunan Province, Dazhou of Sichuan Province, and Bozhou of Anhui Province) were isolated,identified, and analyzed according to the morphology and ITS sequences. Results A total of 3 052 strains of endophytic fungi were isolated from S. ningpoensis in six localities. A total of 84 fungal taxa were identified according to the morphology and ITS sequences,these strains were belong to 25 genera. Thereinto Diaporthe, Fusarium, Nigrospora, Alternaria, Phoma, Corynespora, Epicoccum, and Cladosporium were common genera to S. ningpoensis from six localities, but the dominant genus was different: Fusarium was the dominant genus from Anhui, Hubei, Hunan, and Shaanxi; Alternaria was the dominant genus from Sichuan; Diaporthe was the dominant genus from Zhejiang. According to the similarity coefficient, the composition of the endophytic fungi was distinctly different between six localities. The Shannon-wiener diversity index and Simpson index of endophytic fungi in S. ningpoensis from Zhejiang were found higher than others. The diversity of endophytic fungi in S. ningpoensis from Sichuan was lower than others. Conclusion Systematic studying the diversity and community structure of endophytic fungi in S. ningpoensis and clarifying their distribution regularity in plant tissues can offer basic data and scientific basis for their development and utilization.
引文
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[18]蓝江林,刘波,朱育菁,等.茄子植物内生细菌群落结构与多样性[J].生态环境学报,2009,18(4):1433.
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[20]Higgins K L,Arnold A E,Miadlikowska J,et al.Phylogenetic relationships,host affinity,and geographic structure of boreal and arctic endophytes from three major plant lineages[J].Mol Phylogenet Evol,2007,42(2):543-555.
[21]Khidir H,Eudy D,Porras-Alfaro A,et al.Sinsabaugh.Ageneral suite of fungal endophytes dominate the roots of two dominant grasses in a semiarid grassland[J].JArid Envir,2010,74(1):35-42.
[22]Hoffman M T,Arnold A E.Geographic locality and host identity shape fungal endophyte communities in cupressaceous trees[J].Mycol Res,2008,112(Pt 3):331-344.
[23]Osono T.Endophytic and epiphytic phyllosphere fungi of Camellia japonica:Seasonal and leafage-dependent variations[J].Mycologia,2008,100(3):387-391.
[24]Wu Y,Lu C,Qian X,et al.Diversities with in genotypes,bioactivity and biosynthetic genes of endophytic actinomycetes isolated from three pharmaceutical plants[J].Curr Microbiol,2009,59(4):475-482.
[25]Murali T S,Suryanarayanan T S,Venkatesan G.Fungal endophyte communities in two tropical forests of southern India:Diversity and host affiliation[J].Mycol Prog,2007,6(3):191-199.
[26]Petrini O,Stone J,Carroll F E.Endophytic fungi in evergreen shrubs in western oregon:A preliminary study[J].Can J Bot,1982,60(6):789-796.
[27]Summerell B A,Leslie J F,Backhouse D,et al.Fusarium Paul E.Nelson Memorial Symposium[M].Minnesota:The American Phytopathological Society Press,2001.
[28]Moss M O,Smith J E.The Applied Mycology of Fusarium[M].Cambridge:Cambridge University Press,1984.
[29]马养民,田从丽,张弘弛,等.杜仲内生真菌的分离鉴定及抗菌活性筛选[J].时珍国医国药,2011,22(3):552-557.
[30]张庆波,陈玉婵,李浩华,等.南方红豆杉内生真菌的分离鉴定及其细胞毒活性研究[J].生物技术通报,2013,(1):172-177.
[31]Botella L,Diez J J.Phylogenic diversity of fungal endophytes in Spanish stands of Pinus halepensis[J].Fung Div,2011,47(1):9-18.
[32]Jsaka M,Jaturapat A,Rukseree K,et al.Phomaoxanthones A and B,novel xanthone dimers from the endophytic fungus Phomopsis species[J].J Nat Prod,2011,64(8):1015-1018.
[33]Vesterlund S R,Helander M,Faech S H,et al.Environmental conditions and host plant origin override endophyte effects on invertebrate communities[J].Fung Div,2011,47:109-118.
[2]宋旭红,谭均,潘媛,等.连作对玄参产量和根际土壤肥力及酶活性的影响[J].中药材,2017,40(6):1243-1248.
[3]吴中宝,杨成前,邓才富,等.重庆玄参叶部病害的发生危害及药剂防治效果[J].中国现代中药,2017,19(11):1585-1598.
[4]Rodriguez R J,Jfw J R,Arnold A E,et al.Fungal endophytes:Diversity and function roles[J].New Phytol,2009,182(2):314-330.
[5]Gu X J,Ren K,Yao N,et al.Chemical constituents from endophytic fungus Plectosphaerella cucumerina YCTA2Z1 of Cynanchum auriculatum[J].Chin Herb Med,2018,10(1):95-98.
[6]勇应辉,戴传超,高伏康,等.大戟内生真菌对其生长和两种萜类物质量的影响[J].中草药,2009,30(7):1136-1139.
[7]江曙,陈代杰,戈梅,等.药用植物内生真菌抗菌活性的筛选[J].药物生物技术,2006,13(5):35l-354.
[8]朱虹,单淑芳,李增智,等.苦楝内生真菌及其代谢产物的杀虫活性[J].中国生物防治,2010,26(1):47-52.
[9]宋文玲,刘晓珍,蔡信之,等.2株内生真菌对菊花抗旱特性的影响[J].中国中药杂志,2011,36(3):302-306.
[10]刘晓珍,宋文玲,蔡信之,等.两株内生真菌对菊花抗盐特性的影响[J].中草药,2011,42(1):158-163.
[11]伍晓丽,宋旭红,杨宏国,等.玄参内生真菌分离、鉴定及系统发育分析[J].中国中药杂志,2017,42(5):902-911.
[12]Chen J,Zhang L C,Xing Y M,et al.Diversity and taxonomy of endophytic xylariaceous fungi from medicinal plants of Dendrobium(Orchidaceae)[J].PLoSOne,2013,8(3):e58268.
[13]Collado J,Platas G,GonzáLez I,et al.Geographical and seasonal influences on the distribution of fungal endophytes in Quercus ilex[J].New Phytol,1999,144(3):525-532.
[14]江曙,段金廒,陶金华,等.明党参内生真菌种群的生态分布及其诱导子活性研究[J].中草药,2010,41(1):121-125.
[15]Mishra A,Gond S K,Kumar A,et al.Season and tissue type affect fungal endophyte communities of the Indian medicinal plant Tinospora cordifolia more strongly than geographic location[J].Microb Ecol,2012,64(2):388-398.
[16]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[17]胡克兴,侯晓强,郭顺星.铁皮石斛内生真菌分布[J].微生物学通报,2010,37(1):37-42.
[18]蓝江林,刘波,朱育菁,等.茄子植物内生细菌群落结构与多样性[J].生态环境学报,2009,18(4):1433.
[19]Rivera-Ordu?a F N,Suarez-Sanchez R A,Flores-Bustamante Z R,et al.Diversity of endophytic fungi of Taxus globosa(Mexican yew)fungal[J].Divers,2011,47(1):65-74.
[20]Higgins K L,Arnold A E,Miadlikowska J,et al.Phylogenetic relationships,host affinity,and geographic structure of boreal and arctic endophytes from three major plant lineages[J].Mol Phylogenet Evol,2007,42(2):543-555.
[21]Khidir H,Eudy D,Porras-Alfaro A,et al.Sinsabaugh.Ageneral suite of fungal endophytes dominate the roots of two dominant grasses in a semiarid grassland[J].JArid Envir,2010,74(1):35-42.
[22]Hoffman M T,Arnold A E.Geographic locality and host identity shape fungal endophyte communities in cupressaceous trees[J].Mycol Res,2008,112(Pt 3):331-344.
[23]Osono T.Endophytic and epiphytic phyllosphere fungi of Camellia japonica:Seasonal and leafage-dependent variations[J].Mycologia,2008,100(3):387-391.
[24]Wu Y,Lu C,Qian X,et al.Diversities with in genotypes,bioactivity and biosynthetic genes of endophytic actinomycetes isolated from three pharmaceutical plants[J].Curr Microbiol,2009,59(4):475-482.
[25]Murali T S,Suryanarayanan T S,Venkatesan G.Fungal endophyte communities in two tropical forests of southern India:Diversity and host affiliation[J].Mycol Prog,2007,6(3):191-199.
[26]Petrini O,Stone J,Carroll F E.Endophytic fungi in evergreen shrubs in western oregon:A preliminary study[J].Can J Bot,1982,60(6):789-796.
[27]Summerell B A,Leslie J F,Backhouse D,et al.Fusarium Paul E.Nelson Memorial Symposium[M].Minnesota:The American Phytopathological Society Press,2001.
[28]Moss M O,Smith J E.The Applied Mycology of Fusarium[M].Cambridge:Cambridge University Press,1984.
[29]马养民,田从丽,张弘弛,等.杜仲内生真菌的分离鉴定及抗菌活性筛选[J].时珍国医国药,2011,22(3):552-557.
[30]张庆波,陈玉婵,李浩华,等.南方红豆杉内生真菌的分离鉴定及其细胞毒活性研究[J].生物技术通报,2013,(1):172-177.
[31]Botella L,Diez J J.Phylogenic diversity of fungal endophytes in Spanish stands of Pinus halepensis[J].Fung Div,2011,47(1):9-18.
[32]Jsaka M,Jaturapat A,Rukseree K,et al.Phomaoxanthones A and B,novel xanthone dimers from the endophytic fungus Phomopsis species[J].J Nat Prod,2011,64(8):1015-1018.
[33]Vesterlund S R,Helander M,Faech S H,et al.Environmental conditions and host plant origin override endophyte effects on invertebrate communities[J].Fung Div,2011,47:109-118.