化妆品中六价铬的离子色谱-紫外检测法
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摘要
目的建立化妆品中六价铬的离子色谱-紫外检测器测定方法。方法样品经0.05 mol/L氢氧化钠-磷酸盐缓冲液超声提取后离心,经阴离子色谱柱IonPac AS7(4 mm×250 mm)分离,采用150 mmol/L氯化铵-60 mmol/L氨水溶液为淋洗液,流速为0.8 ml/min,以紫外检测器(375 nm)检测。结果该方法线性范围为0.005～1 mg/L,回归方程为y=18.923x-0.011 7,r>0.999 9。方法检出限为0.04 mg/kg,定量下限为0.12 mg/kg,平均回收率为88.2%～98.8%,RSD为2.24%～4.50%。结论该方法操作简便,具有较好的精密度和准确度,适用于化妆品中六价铬的检测。
Objective To establish an analytical method for the determination of hexavalent chromium in cosmetics by ion chromatography with UV detector. Methods The cosmetics samples were treated by ultrasonic extraction with 0.05 mol/L sodium hydroxide +phosphate buffer solution and then centrifuged,hexavalent chromium in extract were separated by anion chromatography column IonPac AS7(4 mm ×250 mm),with mobile phase of 150 mmol/L ammonium chloride-60 mmol/L ammonia solution,the flow rate was 0.8 ml/min,The UV detection wavelength was 375 nm. Results A good linear relationship showed in the range of 0.005-1 mg/L,and the regression equation was y=18.923 x-0.011 7,r>0.999 9. The limit of detection was 0.04 mg/kg,the limit of quantification was 0.12 mg/kg,the average recovery rates were 88.2%-98.8%,RSDs were 2.24%-4.50%. Conclusion This method is simple,accurate,precise and applicable to the determination of hexavalent chromium in cosmetics
Objective To establish an analytical method for the determination of hexavalent chromium in cosmetics by ion chromatography with UV detector. Methods The cosmetics samples were treated by ultrasonic extraction with 0.05 mol/L sodium hydroxide +phosphate buffer solution and then centrifuged,hexavalent chromium in extract were separated by anion chromatography column IonPac AS7(4 mm ×250 mm),with mobile phase of 150 mmol/L ammonium chloride-60 mmol/L ammonia solution,the flow rate was 0.8 ml/min,The UV detection wavelength was 375 nm. Results A good linear relationship showed in the range of 0.005-1 mg/L,and the regression equation was y=18.923 x-0.011 7,r>0.999 9. The limit of detection was 0.04 mg/kg,the limit of quantification was 0.12 mg/kg,the average recovery rates were 88.2%-98.8%,RSDs were 2.24%-4.50%. Conclusion This method is simple,accurate,precise and applicable to the determination of hexavalent chromium in cosmetics
引文
[1]陈志蓉,张庆生.六价铬的危害性评价及其检测回顾[J].中国药事,2012,26(7):683-687.
[2]Petrucci F,Senofonte O.Determination of Cr(Ⅵ)in cosmetic products using ion chromatography with dynamic reaction cell-inductively coupled plasma-mass spectrometry(DRC-ICP-MS)[J].Anal Methods,2015,7:5269-5274.
[3]Kang EK,Lee S,Park JH,et al.Determination of hexavalent chromium in cosmetic products by ion chromatography and postcolumn derivatization[J].Contact Dermatitis,2006,54:244-248.
[4]李钟瑞,李霞,陆智.化妆品中高关注物质安全性评价[J].日用化学品科学,2016,36(10):38-42.
[5]Hwang M,Yoon EK,Kim JY,Son BK,et al.Safety assessment of chromium by exposure from cosmetic products[J].Arch Pharm Res,2009,32:235-241.
[6]郭少飞,王鹏,王帆,等.高效液相色谱一电感耦合等离子体质谱法测定粉质化妆品中的六价铬[J].检验检疫学刊,2012,22(4):8-13.
[7]钟志雄,朱炳辉,吴西梅.化妆品及保健品中Cr3+和Cr6+的柱后衍生一离子色谱法测定[J].分析测试学报,2008,27(5):546-548.
[8]周凌云,黄坚,宁左云,蔡玲,李春香.浊点萃取-FAAS法测定化妆品中六价铬[J].广州化工,2011,39(4):1-3,10.
[9]USEPA.Method 3060A:alkaline digestion of hexavalent chromium,Test Methods for Evaluating Solid Waste,Physical-Chemical Methods-SW-846[S].USEPA,Washington,DC,1996.
[10]NIOSH.Chromium hexavalent by ion chromatography:Method 7605[S]//NIOSH Manual of Analytical Methods,5th ed,Issue 2,OH:U.S.Department of Health and Human Services,Centers for Disease Control and Prevention,National Institute for Occupational Safety and Health,2017.
[2]Petrucci F,Senofonte O.Determination of Cr(Ⅵ)in cosmetic products using ion chromatography with dynamic reaction cell-inductively coupled plasma-mass spectrometry(DRC-ICP-MS)[J].Anal Methods,2015,7:5269-5274.
[3]Kang EK,Lee S,Park JH,et al.Determination of hexavalent chromium in cosmetic products by ion chromatography and postcolumn derivatization[J].Contact Dermatitis,2006,54:244-248.
[4]李钟瑞,李霞,陆智.化妆品中高关注物质安全性评价[J].日用化学品科学,2016,36(10):38-42.
[5]Hwang M,Yoon EK,Kim JY,Son BK,et al.Safety assessment of chromium by exposure from cosmetic products[J].Arch Pharm Res,2009,32:235-241.
[6]郭少飞,王鹏,王帆,等.高效液相色谱一电感耦合等离子体质谱法测定粉质化妆品中的六价铬[J].检验检疫学刊,2012,22(4):8-13.
[7]钟志雄,朱炳辉,吴西梅.化妆品及保健品中Cr3+和Cr6+的柱后衍生一离子色谱法测定[J].分析测试学报,2008,27(5):546-548.
[8]周凌云,黄坚,宁左云,蔡玲,李春香.浊点萃取-FAAS法测定化妆品中六价铬[J].广州化工,2011,39(4):1-3,10.
[9]USEPA.Method 3060A:alkaline digestion of hexavalent chromium,Test Methods for Evaluating Solid Waste,Physical-Chemical Methods-SW-846[S].USEPA,Washington,DC,1996.
[10]NIOSH.Chromium hexavalent by ion chromatography:Method 7605[S]//NIOSH Manual of Analytical Methods,5th ed,Issue 2,OH:U.S.Department of Health and Human Services,Centers for Disease Control and Prevention,National Institute for Occupational Safety and Health,2017.