HPLC法同时测定野菊花配方颗粒中绿原酸、咖啡酸和蒙花苷含量
|
摘要
目的 :建立高效液相色谱法(HPLC)同时测定野菊花配方颗粒中绿原酸、咖啡酸和蒙花苷的含量。方法:采用高效液相法,色谱柱为Intertsil ODS3 C18柱(4.6 mm×250 mm,5μm);流动相为甲醇-0.1%磷酸水梯度洗脱;检测波长为334 nm,柱温为25℃。结果 :咖啡酸、绿原酸和蒙花苷的浓度分别在0.0624～0.998、0.01268～0.2029、0.1472～1.472μg范围内与峰面积呈良好的线性关系,相关系数分别为0.99998、0.99985和0.99984;平均回收率分别为100.3%、100.8%、100.9%,相对标准偏差值(RSD)分别为1.5%、2.0%、1.8%。结论 :本方法简便、快捷,结果准确,重复性好,可作为野菊花配方颗粒的含量测定方法。
Objective :To establish an HPLC method for the content determination of chlorogenic acid,caffeic acid and linarin in Chrysanthemiindiciflos formula granules. Methods :Determined by HPLC,the analytical method was carried out with Intertsil ODS3 C18(4.6 mm×250 mm,5 μm)columnand. The mobile phase was consisted of methanol-0.1% phosphoric acid solution as a flow rate of 1.0 mL/min. The detection wave length was 334 nm. Results :The calibration curve was linear within the range of 0.0624-0.998μg(r=0.99998),0.01268-0.2029μg(r=0.99985)and 0.1472～1.472 μg(r=0.99984)for chlorogenic acid,caffeic acid and linarin respectively. The average recoveries were100.3%(RSD=1.5%),100.8%(RSD=2.0%)and 100.9%(RSD=1.8%)for chlorogenic acid,caffeic acid and linarin respectively. Conclusion :The method is simple,accurate,reproducible and precise,and applicable for the determination of chlorogenic acid,caffeic acid and linarin in Chrysanthemiindiciflos formula granules.
Objective :To establish an HPLC method for the content determination of chlorogenic acid,caffeic acid and linarin in Chrysanthemiindiciflos formula granules. Methods :Determined by HPLC,the analytical method was carried out with Intertsil ODS3 C18(4.6 mm×250 mm,5 μm)columnand. The mobile phase was consisted of methanol-0.1% phosphoric acid solution as a flow rate of 1.0 mL/min. The detection wave length was 334 nm. Results :The calibration curve was linear within the range of 0.0624-0.998μg(r=0.99998),0.01268-0.2029μg(r=0.99985)and 0.1472～1.472 μg(r=0.99984)for chlorogenic acid,caffeic acid and linarin respectively. The average recoveries were100.3%(RSD=1.5%),100.8%(RSD=2.0%)and 100.9%(RSD=1.8%)for chlorogenic acid,caffeic acid and linarin respectively. Conclusion :The method is simple,accurate,reproducible and precise,and applicable for the determination of chlorogenic acid,caffeic acid and linarin in Chrysanthemiindiciflos formula granules.
引文
[1]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:295.
[2]王德胜,黄艳梅,石岩,等.菊花化学成分及药理作用研究进展[J].安徽农业科学,2018,46(23):9-11,17.
[3]韩正洲,杨勇,贾红梅,等.基于植物代谢组学的栽培型与野生型野菊花的化学成分比较及定量分析[J].药物分析杂志,2017,37(7):1196-1206.
[4]张鞍灵,马琼,高锦明.绿原酸及其类似物与生理作用[J].中草药,2001,32(2):173.
[5]张敏.菊花茎叶总黄酮、绿原酸提取纯化及抗氧化活性研究[D].济南:山东中医药大学,2015.
[6]田雪菲,蒲小萍.咖啡酸对神经毒素MPP+诱发大鼠小脑颗粒细胞凋亡的保护作用[J].北京大学学报:医学版,2004,36(1):27.
[7]戴树培.密蒙花有效成分提取及含量测定方法[J].西北药学杂志,1996,11(5):204.
[8]甄亚钦,田伟,任艳青,等.野菊花饮片、标准煎液、配方颗粒UPLC特征图谱相关性研究[J].中国药师,2018,21(7):1162-1166.
[9]方红玫,周秋亚.HPLC-DAD法同时测定小蓟配方颗粒中5种活性成分的含量[J].中国药师,2018,21(6):1125-1128.
[10]成杰,杨鹏飞,何康,等.HPLC法同时测定维吾尔药材百里香中6种成分的含量[J].药物分析杂志,2016,36(2):285-290.
[11]黄丽.HPLC-DAD法同时测定中药小蓟中6种活性成分的含量[J].中医药导报,2016,22(4):66-68,74.
[2]王德胜,黄艳梅,石岩,等.菊花化学成分及药理作用研究进展[J].安徽农业科学,2018,46(23):9-11,17.
[3]韩正洲,杨勇,贾红梅,等.基于植物代谢组学的栽培型与野生型野菊花的化学成分比较及定量分析[J].药物分析杂志,2017,37(7):1196-1206.
[4]张鞍灵,马琼,高锦明.绿原酸及其类似物与生理作用[J].中草药,2001,32(2):173.
[5]张敏.菊花茎叶总黄酮、绿原酸提取纯化及抗氧化活性研究[D].济南:山东中医药大学,2015.
[6]田雪菲,蒲小萍.咖啡酸对神经毒素MPP+诱发大鼠小脑颗粒细胞凋亡的保护作用[J].北京大学学报:医学版,2004,36(1):27.
[7]戴树培.密蒙花有效成分提取及含量测定方法[J].西北药学杂志,1996,11(5):204.
[8]甄亚钦,田伟,任艳青,等.野菊花饮片、标准煎液、配方颗粒UPLC特征图谱相关性研究[J].中国药师,2018,21(7):1162-1166.
[9]方红玫,周秋亚.HPLC-DAD法同时测定小蓟配方颗粒中5种活性成分的含量[J].中国药师,2018,21(6):1125-1128.
[10]成杰,杨鹏飞,何康,等.HPLC法同时测定维吾尔药材百里香中6种成分的含量[J].药物分析杂志,2016,36(2):285-290.
[11]黄丽.HPLC-DAD法同时测定中药小蓟中6种活性成分的含量[J].中医药导报,2016,22(4):66-68,74.