木质素酶法处理含油废水的研究
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摘要
本实验研究以模拟含柴油废水为研究对象,研究了木质素酶对含柴油污水中柴油的酶催化氧化降解情况。考察了溶液pH值、催化时间、木质素酶浓度、反应温度、柴油初始浓度等不同要素对酶催化氧化效果的影响。通过正交实验确定最佳实验条件为:柴油初始浓度为0.3 g·L~(-1),木质素酶浓度为12 mg·L~(-1),pH值为5.5,反应温度为30℃,反应时间为3h,柴油的去除率可达90.17%。
The simulated wastewater with diesel as object of this study, we do research on using the ligninolytic enzyme to catalyze oxidation it and get circumstance of diesel degradation. The effects on the catalytic oxidation of the enzyme under the different factors, such as solution pH value, catalysis time, concentration of ligninolytic enzyme, reaction temperature and initial concentration of diesel oil, are investigated. Orthogonal experiments determine the following optimum experimental conditions: initial concentration of diesel oil is 0.3 g·L~(-1), the concentration of ligninolytic enzyme is 12 mg·L~(-1), the pH value is 5.5, the reaction temperature is 30 ℃, the reaction time is 3 h, the removal rate of diesel oil is up to 90.17%.
The simulated wastewater with diesel as object of this study, we do research on using the ligninolytic enzyme to catalyze oxidation it and get circumstance of diesel degradation. The effects on the catalytic oxidation of the enzyme under the different factors, such as solution pH value, catalysis time, concentration of ligninolytic enzyme, reaction temperature and initial concentration of diesel oil, are investigated. Orthogonal experiments determine the following optimum experimental conditions: initial concentration of diesel oil is 0.3 g·L~(-1), the concentration of ligninolytic enzyme is 12 mg·L~(-1), the pH value is 5.5, the reaction temperature is 30 ℃, the reaction time is 3 h, the removal rate of diesel oil is up to 90.17%.
引文
[1]陈新芳.油对水质稳定剂性能的影响及酶法处理含油污水的研究[D].北京:北京化工大学,2007
[2]陆洪宇,马文成,张梁,等.曝气生物滤池深度处理混合印染废水[J].环境工程学报,2013,7(7):2409-2413
[3]程元辉.浅谈加油站环境污染与预防对策[J].安全、健康和环境,2015,15(6):35-36
[4]王聪.三种方法对水绵(Spirogyra)生长抑制效应的研究[D].上海:上海海洋大学,2012
[5]蔡钊荣.油田含油污水处理及回用技术[D].青岛:中国海洋大学,2006
[6]张彤,黄慧,赵庆祥.造纸废水中有机氯化物的生物酶处理技术研究进展[J].环境科学,1997,18(2):79-83
[7]孟睿.固定化菌-藻体系净化水产养殖废水的研究[D].北京:北京化工大学,2009
[8]邵凤琴,韩庆祥.生物酶工程在污染治理中的应用[J].石油化工高等学校学报,2003,16(2):36-40
[9]孙伟,韩军英,陆路德.过氧化酶催化反应在工业废水处理中的应用[J].环境科学与技术,2003,26(增刊):75-77
[10]Klibanov AM,Alberti BN,Morris ED,et al.Enzymatic Removal of Toxic Phenols and Anilines from Wastewater[J].Journal of applied biochemistry,1980,10(2):414-421
[11]Klibanov AM.Peroxidase-Catalyzed removal of phenols from coal-conversion waste water[J].Science,1983,221(3):259-261
[12]Klibanov AM.Horseradish peroxidase for the removal of carcinogenic aromatics amines from Water[J].Enzyme and Microbial Technology,1981,3(2):119-122
[13]Tien M,Kirth TK.Lignin一Degrading Enzyme from the Hymenomycete Phaenrochaete Chrysosporium Burds[J].Science,1983,221(4):661-663
[14]Huang X,Wang D,Liu CX,et al.The roles of veratryl alcohol and nonionic surfactant in the oxidation of phenolic compounds by lignin peroxidase[J].Biochemical and Biophysical Research Communi-cations,2003,311:491-494
[15]Sajja HK.Developments and trends in enzyme catalysis in nonconventional media[J].Biotechnology Advances,2002,20(4):239-267
[16]左红梅,张华,何春娥.生物酶/ACF复合材料降解甲醛实验研究[J].化工新型材料,2013,41(7):113-115,118
[17]郭星.生物酶提高制浆造纸废水可生化性的研究[D].西安:陕西科技大学,2014
[18]李保梅,乔欣,赵雅琴.生物酶在印染工业中应用进展[J].染整技术,2012,34(2):12-15
[19]刘浩,蔡记华,肖长波.生物酶可降解钻井液降解效果评价方法[J].钻井液与完井液,2012,29(3):74-77,97
[20]陈坤.仿酶型纳米磁性水处理剂的制备及催化机理的研究[D].武汉:武汉科技大学,2014
[21]乔彤森,刘发强,陈涛.酶催化氧化处理高浓度工业有机废水[J].石油技术与应用,2005,23(6):470-471
[22]刘云庆.紫外可见上转换剂/TiO_2光催化剂降解海洋石油污染的研究[D].大连:大连海洋大学,2015
[23]尚伟龙.漆酶在陶瓷-壳聚糖复杂载体上固定化及在含油废水治理中的应用研究[D].北京:北京化工大学,2009
[24]尚晓琳,于晓彩,季秋忆,等.用辣根过氧化物酶催化降解海水中柴油污染的研究[J].大连海洋大学学报,2016,31(1):113-116
[25]鲁时瑛,李华钟,陈坚.EDTA对木质素过氧化物酶在染料脱色中的影响[J].无锡轻工大学学报,2002,21(4):393-39
[26]张剑波,左澎,叶鹏,等.固定化过氧化物酶在过氧化物测定中的应用[J].分析化学,2003,31(1):75-76
[2]陆洪宇,马文成,张梁,等.曝气生物滤池深度处理混合印染废水[J].环境工程学报,2013,7(7):2409-2413
[3]程元辉.浅谈加油站环境污染与预防对策[J].安全、健康和环境,2015,15(6):35-36
[4]王聪.三种方法对水绵(Spirogyra)生长抑制效应的研究[D].上海:上海海洋大学,2012
[5]蔡钊荣.油田含油污水处理及回用技术[D].青岛:中国海洋大学,2006
[6]张彤,黄慧,赵庆祥.造纸废水中有机氯化物的生物酶处理技术研究进展[J].环境科学,1997,18(2):79-83
[7]孟睿.固定化菌-藻体系净化水产养殖废水的研究[D].北京:北京化工大学,2009
[8]邵凤琴,韩庆祥.生物酶工程在污染治理中的应用[J].石油化工高等学校学报,2003,16(2):36-40
[9]孙伟,韩军英,陆路德.过氧化酶催化反应在工业废水处理中的应用[J].环境科学与技术,2003,26(增刊):75-77
[10]Klibanov AM,Alberti BN,Morris ED,et al.Enzymatic Removal of Toxic Phenols and Anilines from Wastewater[J].Journal of applied biochemistry,1980,10(2):414-421
[11]Klibanov AM.Peroxidase-Catalyzed removal of phenols from coal-conversion waste water[J].Science,1983,221(3):259-261
[12]Klibanov AM.Horseradish peroxidase for the removal of carcinogenic aromatics amines from Water[J].Enzyme and Microbial Technology,1981,3(2):119-122
[13]Tien M,Kirth TK.Lignin一Degrading Enzyme from the Hymenomycete Phaenrochaete Chrysosporium Burds[J].Science,1983,221(4):661-663
[14]Huang X,Wang D,Liu CX,et al.The roles of veratryl alcohol and nonionic surfactant in the oxidation of phenolic compounds by lignin peroxidase[J].Biochemical and Biophysical Research Communi-cations,2003,311:491-494
[15]Sajja HK.Developments and trends in enzyme catalysis in nonconventional media[J].Biotechnology Advances,2002,20(4):239-267
[16]左红梅,张华,何春娥.生物酶/ACF复合材料降解甲醛实验研究[J].化工新型材料,2013,41(7):113-115,118
[17]郭星.生物酶提高制浆造纸废水可生化性的研究[D].西安:陕西科技大学,2014
[18]李保梅,乔欣,赵雅琴.生物酶在印染工业中应用进展[J].染整技术,2012,34(2):12-15
[19]刘浩,蔡记华,肖长波.生物酶可降解钻井液降解效果评价方法[J].钻井液与完井液,2012,29(3):74-77,97
[20]陈坤.仿酶型纳米磁性水处理剂的制备及催化机理的研究[D].武汉:武汉科技大学,2014
[21]乔彤森,刘发强,陈涛.酶催化氧化处理高浓度工业有机废水[J].石油技术与应用,2005,23(6):470-471
[22]刘云庆.紫外可见上转换剂/TiO_2光催化剂降解海洋石油污染的研究[D].大连:大连海洋大学,2015
[23]尚伟龙.漆酶在陶瓷-壳聚糖复杂载体上固定化及在含油废水治理中的应用研究[D].北京:北京化工大学,2009
[24]尚晓琳,于晓彩,季秋忆,等.用辣根过氧化物酶催化降解海水中柴油污染的研究[J].大连海洋大学学报,2016,31(1):113-116
[25]鲁时瑛,李华钟,陈坚.EDTA对木质素过氧化物酶在染料脱色中的影响[J].无锡轻工大学学报,2002,21(4):393-39
[26]张剑波,左澎,叶鹏,等.固定化过氧化物酶在过氧化物测定中的应用[J].分析化学,2003,31(1):75-76
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