纯钛二次高压阳极氧化载锌生物膜层生物相容性
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摘要
目的:研究钛片二次高压阳极氧化载锌生物膜层抗菌性以及细胞生物相容性的改变情况。方法:试件二次高压阳极氧化为A组,再经植酸处理的为B组,以B组为基化学镀锌(15g/L、20g/L、25g/L乙酸锌)分别为C、D、E组。将不同级别试样进行细胞黏附、增殖及激光共聚焦显微镜研究细胞相容性。结果:细胞增殖和黏附率均为E>D>C>B>A,且5组数据之间比较差异均具有统计学意义。碱性磷酸酶检测D、E两组细胞ALP吸光度值显著高于A、B、C三组,且E组高于D组。结论:纯钛二次高压阳极氧化经植酸处理载25g/L乙酸锌的生物相容性最佳。且随着锌离子浓度增加,生物相容性成正相关。
Objective:To study the titanium plate two high voltage anodic oxidation of zinc loaded antibacterial biological film and changes of cell biocompatibility.Methods:titanium specimens two high voltage anodic oxidation without the phytic acid treatment group A was treated with phytic acid as group B,after two times of high voltage anodic oxidation after chemical processing of phytic acid 15 g/L,20 g/L,25 g/L zinc acetate composite treatment in experimental C,D,E group.The titanium plate two high voltage anodic oxidation by phytic acid were cultured zinc loaded specimens after placed at 37℃,then count the colony and calculate the inhibitory rate;through CCK-8 method and laser confocal microscope were used to study the cytocompatibility of the specimens.Results:the bacteriostatic rate,cell proliferation and adhesion rate were E>D>C>B>A.Laser confocal cytoskeleton morphology showed that the two groups of DNE cells grew faster and more closely related.Conclusion:Confocal cell cytoskeleton morphology of the two groups is more rapid and more closely related.Group E had good biocompatibility,and the best biocompatibility of group E was 25 g/L zinc acetate after phytic acid treatment.
Objective:To study the titanium plate two high voltage anodic oxidation of zinc loaded antibacterial biological film and changes of cell biocompatibility.Methods:titanium specimens two high voltage anodic oxidation without the phytic acid treatment group A was treated with phytic acid as group B,after two times of high voltage anodic oxidation after chemical processing of phytic acid 15 g/L,20 g/L,25 g/L zinc acetate composite treatment in experimental C,D,E group.The titanium plate two high voltage anodic oxidation by phytic acid were cultured zinc loaded specimens after placed at 37℃,then count the colony and calculate the inhibitory rate;through CCK-8 method and laser confocal microscope were used to study the cytocompatibility of the specimens.Results:the bacteriostatic rate,cell proliferation and adhesion rate were E>D>C>B>A.Laser confocal cytoskeleton morphology showed that the two groups of DNE cells grew faster and more closely related.Conclusion:Confocal cell cytoskeleton morphology of the two groups is more rapid and more closely related.Group E had good biocompatibility,and the best biocompatibility of group E was 25 g/L zinc acetate after phytic acid treatment.
引文
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[14]Yamaguchi M.Role of nutritional zinc in the prevention of osteoporosis[J].Molecular&Cellular Biochemistry,2010,338(1/2):242-254.
[2]Das K,Bose S,Bandyopadh yay A.TiO2nanotubes on Ti:Influence of nanoscale morph ology on bone cell-materials interaction[J].J Biomed Mater Res A,2009,90(1):225-237.
[3]Gong DW,Grimes CA,Vargh ese OK,et al.Titanium oxidenanotube arrays prepared by anodic oxidation[J].J Mater Res,2001,16(12):3331-3334.
[4]Daskalaki VM,Fulgione I,et al.Solar light-induced photoelectrocatalytic degradation of bisphenol-A on TiO2/ITO film anode and BDD cath-ode[J].Catal Today,2013,209(15):74-78.
[5]刘雯君.钛金属阳极氧化表面及其复合物的抗菌性[J].中国口腔种植学杂志,2014,19(1):38-39.
[6]刘继光,宋镓岐,李慕勤,等.纯镁超声微弧氧化-植酸-镀锌生物膜层的制备与抗菌性.中国表面工程,2016,12(6):84-87.
[7]刘文菲,张振庭,赵晟楠,等.不同电压阳极氧化处理对金属钛表面形态和羟基磷灰石沉积作用的研究[J].北京口腔医学,2012,20(5):255-256.
[8]魏丹,夏正斌,等.恒电位模式下纯钛阳极氧化膜的形成及结晶特性[J].华南理工大学学报:自然科学版,2012,40(3):30-31.
[9]付晓龙,李莺,李宝娥,等.纯钛表面阳极氧化增强成骨细胞生物活性及护骨素基因的表达[J].中国组织工程研究,2014,18(39):6243-6244.
[10]宁成云,王玉强,郑华德,等.阳极氧化法制备二氧化钛纳米管阵列的研究[J].化学研究与应用,2010,22(1):14-17.
[11]Yang B,Ng CK,Fung MK,et al.Annealing study of titanium oxide nanotube arrays[J].Matetials Chemisty and Physics,2011,130(3):1227-1231.
[12]肖月,陈洪伟,等.纯镁超声微弧氧化-植酸-载锌复合膜层的细胞相容性[J].中国体视学与图像分析,2016,21(1):118.
[13]张兵.纯钛表面载锌的微弧氧化生物活性陶瓷膜制备及性能研究[D].哈尔滨:哈尔滨工程大学硕士论文,2014.
[14]Yamaguchi M.Role of nutritional zinc in the prevention of osteoporosis[J].Molecular&Cellular Biochemistry,2010,338(1/2):242-254.