羟基磷灰石/壳聚糖复合层的制备及其结合力、润湿性研究
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摘要
通过微弧氧化处理,在Ti植入体表面制备了兼具花瓣和多孔形貌的羟基磷灰石(HA)涂层,而后通过浸渍-提拉法又在HA表面成功装载了不同浓度的壳聚糖(CS)。对所得复合层进行SEM、EDS、XRD检测,探究负载CS前后复合层的的形貌、组成及物相变化;通过FT-IR分析复合层所含官能团及HA/CS界面的相互作用;并对不同HA/CS样品进行接触角测试,探究不同CS浓度对样品表面润湿性的影响;通过划痕法测试CS与HA的界面结合强度。结果表明,随着CS浓度增大,可逐渐遮盖HA层的花瓣、多孔形貌特征,使得钛表面形成光滑、连续的CS膜层;HA和CS之间有一定的键合作用,界面结合力可达8.3N;CS的负载可有效降低HA层的表面润湿性,且随着CS浓度增大,表面润湿性不断降低。
In this work,porous hydroxyapatite(HA)coating with flower-shaped morphology was prepared on the surface of Ti implants by micro-arc oxidation treatment,and then different concentrations of chitosan(CS)were successfully loaded on the HA surface through dip-coating method.SEM,EDS and XRD tests were performed to investigate the morphology,elemental composition and physical phase of the coatings before and after CS loading.FT-IR was used to detect the functional groups and their interaction within the coatings.The wettability of the different HA/CS samples was evaluated by measuring the contact angles with deionized water.And the interface bonding strength between CS and HA was tested by scratch method.The results showed that,with the increase of CS concentration,the flower-shaped HA coatings were gradually covered,finally forming a smooth and continuous CS layer on Ti surface.The interface bonding strength could be up to 8.3 Ndue to the interactions between HA and CS.The load of CS could effectively reduce the surface wettability of the HA layer,and the surface wettability decreased with the increase of CS concentration.
In this work,porous hydroxyapatite(HA)coating with flower-shaped morphology was prepared on the surface of Ti implants by micro-arc oxidation treatment,and then different concentrations of chitosan(CS)were successfully loaded on the HA surface through dip-coating method.SEM,EDS and XRD tests were performed to investigate the morphology,elemental composition and physical phase of the coatings before and after CS loading.FT-IR was used to detect the functional groups and their interaction within the coatings.The wettability of the different HA/CS samples was evaluated by measuring the contact angles with deionized water.And the interface bonding strength between CS and HA was tested by scratch method.The results showed that,with the increase of CS concentration,the flower-shaped HA coatings were gradually covered,finally forming a smooth and continuous CS layer on Ti surface.The interface bonding strength could be up to 8.3 Ndue to the interactions between HA and CS.The load of CS could effectively reduce the surface wettability of the HA layer,and the surface wettability decreased with the increase of CS concentration.
引文
[1]Okada T,Nobunaga Y,Konishi T,et al.Preparation of chitosan-hydroxyapatite composite mono-fiber using coagulation method and their mechanical properties[J].Carbohydr Polym,2017,175:355-360.
[2]Zhao Hongxia.Process of study on drug-loaded chitosan/hydroxyapatite composite in bone tissue engineering[J].Journal of Founctional Materials,2014(13):13006-13012.赵宏霞.载药壳聚糖/羟基磷灰石复合材料在骨组织工程中的研究进展[J].功能材料,2014(13):13006-13012.
[3]Xu Ting,He Di,Zhou Yinyin,et al.Progress of hydroxyapatite/chitosan biomedical composite[J].Apaedic Biomechanics Materials and Clinical Study,2008,5(1):44-48.徐挺,何狄,周银银,等.羟基磷灰石/壳聚糖生物复合材料的制备研究进展[J].生物骨科材料与临床研究,2008,5(1):44-48.
[4]Chen Zhizhou,Nian Jianlou,Zang Rui,et al.Chitosan filming and its applications in food storage and packaging[J].Journal of Cellulose Science and Technology,2004,12(3):43-47(in Chinese).陈志周,牟建楼,臧蕊,等.壳聚糖的成膜性及其在食品保鲜、包装上的应用[J].纤维素科学与技术,2004,12(3):43-47.
[5]Hu Yimin,Chen Jingdi.Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nanohydroxyapatite/chitosan/chondroitin sulfate hyaluronic acid for bone tissue engineering[J].Colloids and Surfaces B:Biointerfaces,2017,157:93-100.
[6]Chen Zhizhou,Mu Jianlou,Zang Rui,et al.Chitosan filming and its applications in food storage and packaging[J].Journal of Cellulose Science and Technology,2004,12(3):43-47.陈志周,牟建楼,臧蕊,等.壳聚糖的成膜性及其在食品保鲜、包装上的应用[J].纤维素科学与技术,2004,12(3):43-47.
[7]Wei X,Fu C,Savino K,et al.Carbonated hydroxyapatite coatings with aligned crystal domains[J].Crystal Growth&Design,2012,1(12):3474-3480.
[8]Baboucarr Lowe,Jayachandran Venkatesan,Sukumaran Anil,et al.Preparation and characterization of chitosannatural nano hydroxyapatite-fucoidan nanocomposites for bone tissue engineering[J].International Journal of Biological Macromolecules,2016,93(Pt B):1479.
[9]Khalajabadi S Z,Ahmad N,Izman S,et al.In vitro,biodegradation,electrochemical corrosion evaluations and mechanical properties of an Mg/HA/TiO2,nanocomposite for biomedical applications[J].Journal of Alloys&Compounds,2016,696.
[10]Branca C,D’Angelo G,Crupi C,et al.Role of the OHand NH vibrational groups in polysaccharide-nanocomposite interactions:A FT-IR-ATR study on chitosan and chitosan/clay films[J].Polymer,2016,99:614-622.
[11]Moussout H,Ahlafi H,Aazza M,et al.Kinetics and mechanism of the thermal degradation of biopolymers chitin and chitosan using thermogravimetric analysis[J].Polymer Degradation&Stability,2016,130:1-9.
[12]Chokradjaroen C,Rujiravanit R,Watthanaphanit A,et al.Enhanced degradation of chitosan by applying plasma treatment in combination with oxidizing agents for potential use as an anticancer agent[J].Carbohydrate Polymers,2017,167:1-11.
[13]Ma Xiangyu,Feng Yafei,Ma Zhensheng,et al.The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces[J].Biomaterials,35(2014):7259-7270.
[14]Liu J,Chen X,Shao Z,et al.Preparation and characterization of chitosan/Cu(Ⅱ)affinity membrane for urea adsorption[J].Journal of Applied Polymer Science,2003,90(12):1108-1112.
[15]Chen Yunfu,Chen Yongping,Zhang Chengbin,et al.Influence of rough surface topogarphy on wettability[J].Journal of Engineering Thermophysics,2011,32(7):1188-1190(in Chinese).陈云富,陈永平,张程宾,等.粗糙表面形貌对湿润性的影响[J].工程热物理学报,2011,32(7):1188-1190.
[2]Zhao Hongxia.Process of study on drug-loaded chitosan/hydroxyapatite composite in bone tissue engineering[J].Journal of Founctional Materials,2014(13):13006-13012.赵宏霞.载药壳聚糖/羟基磷灰石复合材料在骨组织工程中的研究进展[J].功能材料,2014(13):13006-13012.
[3]Xu Ting,He Di,Zhou Yinyin,et al.Progress of hydroxyapatite/chitosan biomedical composite[J].Apaedic Biomechanics Materials and Clinical Study,2008,5(1):44-48.徐挺,何狄,周银银,等.羟基磷灰石/壳聚糖生物复合材料的制备研究进展[J].生物骨科材料与临床研究,2008,5(1):44-48.
[4]Chen Zhizhou,Nian Jianlou,Zang Rui,et al.Chitosan filming and its applications in food storage and packaging[J].Journal of Cellulose Science and Technology,2004,12(3):43-47(in Chinese).陈志周,牟建楼,臧蕊,等.壳聚糖的成膜性及其在食品保鲜、包装上的应用[J].纤维素科学与技术,2004,12(3):43-47.
[5]Hu Yimin,Chen Jingdi.Biomimetic mineralized hierarchical hybrid scaffolds based on in situ synthesis of nanohydroxyapatite/chitosan/chondroitin sulfate hyaluronic acid for bone tissue engineering[J].Colloids and Surfaces B:Biointerfaces,2017,157:93-100.
[6]Chen Zhizhou,Mu Jianlou,Zang Rui,et al.Chitosan filming and its applications in food storage and packaging[J].Journal of Cellulose Science and Technology,2004,12(3):43-47.陈志周,牟建楼,臧蕊,等.壳聚糖的成膜性及其在食品保鲜、包装上的应用[J].纤维素科学与技术,2004,12(3):43-47.
[7]Wei X,Fu C,Savino K,et al.Carbonated hydroxyapatite coatings with aligned crystal domains[J].Crystal Growth&Design,2012,1(12):3474-3480.
[8]Baboucarr Lowe,Jayachandran Venkatesan,Sukumaran Anil,et al.Preparation and characterization of chitosannatural nano hydroxyapatite-fucoidan nanocomposites for bone tissue engineering[J].International Journal of Biological Macromolecules,2016,93(Pt B):1479.
[9]Khalajabadi S Z,Ahmad N,Izman S,et al.In vitro,biodegradation,electrochemical corrosion evaluations and mechanical properties of an Mg/HA/TiO2,nanocomposite for biomedical applications[J].Journal of Alloys&Compounds,2016,696.
[10]Branca C,D’Angelo G,Crupi C,et al.Role of the OHand NH vibrational groups in polysaccharide-nanocomposite interactions:A FT-IR-ATR study on chitosan and chitosan/clay films[J].Polymer,2016,99:614-622.
[11]Moussout H,Ahlafi H,Aazza M,et al.Kinetics and mechanism of the thermal degradation of biopolymers chitin and chitosan using thermogravimetric analysis[J].Polymer Degradation&Stability,2016,130:1-9.
[12]Chokradjaroen C,Rujiravanit R,Watthanaphanit A,et al.Enhanced degradation of chitosan by applying plasma treatment in combination with oxidizing agents for potential use as an anticancer agent[J].Carbohydrate Polymers,2017,167:1-11.
[13]Ma Xiangyu,Feng Yafei,Ma Zhensheng,et al.The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces[J].Biomaterials,35(2014):7259-7270.
[14]Liu J,Chen X,Shao Z,et al.Preparation and characterization of chitosan/Cu(Ⅱ)affinity membrane for urea adsorption[J].Journal of Applied Polymer Science,2003,90(12):1108-1112.
[15]Chen Yunfu,Chen Yongping,Zhang Chengbin,et al.Influence of rough surface topogarphy on wettability[J].Journal of Engineering Thermophysics,2011,32(7):1188-1190(in Chinese).陈云富,陈永平,张程宾,等.粗糙表面形貌对湿润性的影响[J].工程热物理学报,2011,32(7):1188-1190.
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