星点-响应面法优选阔叶十大功劳叶中小檗碱的提取工艺
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摘要
目的优选阔叶十大功劳叶的小檗碱提取工艺。方法对阔叶十大功劳叶中小檗碱采用超声醇提,利用星点-响应面设计,以盐酸小檗碱含量为评价指标,选取料液比、乙醇浓度、超声时间3个因素进行中心组合设计,通过星点-响应面设计来筛选阔叶十大功劳叶中小檗碱的最优提取工艺。结果阔叶十大功劳叶中小檗碱最优工艺为:液料比13∶1;乙醇浓度54%;超声时间35min,提取2次,经3次验证试验,最佳工艺提取的功劳叶中小檗碱为0.683%(RSD=0.85%,n=3),与预测值0.691%比较,偏差为1.16%。结论星点设计-响应面法优化功劳叶中小檗碱的提取工艺,方法简单合理,且具有良好的预测性。
Objective To optimize the extraction process of berberine from Mahonia bealei leaves by the central composite design-response surface method.Methods The ultrasonic ethanol extraction method was used to extract berberine from Mahonia bealei leaves.The central composite design-response surface design was used to make the central composite design with the three factors of solid-liquid ratio,ethanol concentration and ultrasonic time by taking the content of berberine hydrochloride as the evaluation index and screen the optimal extraction process of berberine from Mahonia bealei leaves.Results The optimal extraction process was as follows:the extraction process was conducted with the solid-liquid ratio of 13:1 and the ethanol concentration of 54% for 35 minutes of the ultrasonic time twice respectively,and the content of berberine in the optimal exaction process was0.683%(RSD=0.85%,n=3)with three validation tests,and the bias ratio was 1.16% when compared with the predicted value of 0.691%.Conclusion The extraction technology of berberine from Mahonia bealei leaves optimized by the central composite design-response surface method is simple and reliable with good predictability.
Objective To optimize the extraction process of berberine from Mahonia bealei leaves by the central composite design-response surface method.Methods The ultrasonic ethanol extraction method was used to extract berberine from Mahonia bealei leaves.The central composite design-response surface design was used to make the central composite design with the three factors of solid-liquid ratio,ethanol concentration and ultrasonic time by taking the content of berberine hydrochloride as the evaluation index and screen the optimal extraction process of berberine from Mahonia bealei leaves.Results The optimal extraction process was as follows:the extraction process was conducted with the solid-liquid ratio of 13:1 and the ethanol concentration of 54% for 35 minutes of the ultrasonic time twice respectively,and the content of berberine in the optimal exaction process was0.683%(RSD=0.85%,n=3)with three validation tests,and the bias ratio was 1.16% when compared with the predicted value of 0.691%.Conclusion The extraction technology of berberine from Mahonia bealei leaves optimized by the central composite design-response surface method is simple and reliable with good predictability.
引文
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[3]余天虹,苏薇薇,容丽.十大功劳属植物研究进展[J].贵州师范大学学报(自然科学版),2015,33(3):115-120.
[4]丛悦,王艳,王天晓,等.功劳木的化学成分研究[J].中成药,2011,33(6):1008-1010.
[5]樊丽博,张晓会,刘兴金,等.狭叶十大功劳化学成分分析[J].中国兽药杂志,2011,45(10):34-36.
[6]张稳,刘杉杉,肖雪峰,等.功劳木生物碱提取工艺研究进展[J].医药论坛杂志,2018,39(8):171-173.
[7]赵丹,邓祖磊,崔田,等.高效液相色谱法同时测定不同产地阔叶十大功劳叶中3种生物碱类成分的含量[J].中国药业,2016,25(15):33-35.
[8]杨昌贵,肖承鸿,江维克,等.十大功劳叶药材的质量标准提高研究[J].中国药房,2018,29(4):470-473.
[9]赵丹.中药十大功劳叶的质量标准研究[D].安徽蚌埠:蚌埠医学院,2017:24.
[10]蒲忠慧,王保山,陈希文,等.功劳叶提取物的体外抗菌活性研究[J].中国兽医杂志,2016,52(1):109-112.
[11]Chao J,Liao J W,Peng W H,et al.Antioxidant,analgesic,anti-inflammatory,and hepatoprotective effects of the ethanol extract of mahonia oiwakensis stem[J].Int J Mol Sci,2013,14(2):2928-2945.
[12]Wang T X,Shi X Y,Cong Y,et al.Reversal of multidrug resistance by 5,5′-dimethoxylariciresinol-4-O-β-D-glucoside in doxorubicin-resistant human leukemia K562/DOX[J].Indian J Pharmacol,2013,45(6):597-602.
[13]李燕婧,钟正贤,陈学芬,等.长柱十大功劳与阔叶十大功劳水提物药理作用比较[J].中医药导报,2010,16(9):92-93,96.
[14]韩伟,刘傲霞,成悦,等.星点设计:效应面优化法在中药提取中的应用[J].机电信息,2013,26:1-5,12.
[15]周秀娟,陈俊可,曾锐.响应面法优化超声辅助提取粗茎秦艽多糖的工艺研究[J].中药材,2016,39(11):2572-2575.
[16]辛明慧,张荣华,杨丽,等.响应面法优化辣木中多糖和芦丁的超声提取工艺[J].中成药,2017,39(3):625-628.