柴胡黄芩药对的基础研究探析
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摘要
柴胡和黄芩是临床上常见的中药配伍,是《伤寒论》中最具代表意义的药对配伍之一,作为经典药对广泛运用于呼吸系统、消化系统、心血管系统和神经系统疾病的复方治疗中。研究发现,柴胡黄芩药对配伍可以提高柴胡皂苷和黄芩苷的溶出量,这可能该是药对配伍药效增强的物质基础。现代药理研究表明,柴黄药对具有缓解肝损伤、抗肝纤维化、抗病毒、解热抗炎、治疗肝癌、利胆和抗癫痫等作用。本文从配伍后主要药效成分溶出研究和药理作用等方面对柴胡黄芩药对的文献进行综述,以期为其深入开发提供参考。
Radix Bupleuri and Radix Scutellariae are common herb pair of traditional Chinese medicine in clinical practice, and is also one of the most representative compatibility of drugs in the Treatise on Febrile Diseases. As a classic medicine suits, they are widely used in the treatment of respiratory system, digestive system, cardiovascular system and nervous system diseases. It was found that the compatibility of Radix Bupleuri with Radix Scutellariae improved the dissolution of Saikoside and Baicalin, which may be the material basis for enhancing the efficacy of this medicine suits.Modern pharmacological studies have shown that Chaihuang herb pair has the functions of relieving liver injury, antiliver fibrosis, anti-virus, antipyretic and anti-inflammation, treating liver cancer, choleretic action and anti-epilepsy.This paper reviews the literatures on Chaihuang herb pair from the aspects of dissolution of main medicinal ingredients and pharmacological action after compatibility, so as to provide references for its further development.
Radix Bupleuri and Radix Scutellariae are common herb pair of traditional Chinese medicine in clinical practice, and is also one of the most representative compatibility of drugs in the Treatise on Febrile Diseases. As a classic medicine suits, they are widely used in the treatment of respiratory system, digestive system, cardiovascular system and nervous system diseases. It was found that the compatibility of Radix Bupleuri with Radix Scutellariae improved the dissolution of Saikoside and Baicalin, which may be the material basis for enhancing the efficacy of this medicine suits.Modern pharmacological studies have shown that Chaihuang herb pair has the functions of relieving liver injury, antiliver fibrosis, anti-virus, antipyretic and anti-inflammation, treating liver cancer, choleretic action and anti-epilepsy.This paper reviews the literatures on Chaihuang herb pair from the aspects of dissolution of main medicinal ingredients and pharmacological action after compatibility, so as to provide references for its further development.
引文
1唐于平,束晓云,李伟霞,等.药对研究(Ⅰ)--药对的形成与发展.中国中药杂志,2013,38(24):4185-4190.
2张智华.《伤寒论》柴胡药对探析.湖北中医杂志,2014,36(1):47-48.
3吕富银.黄芩常用药对浅述.世界中医药,2010,5(5):353-354.
4吕晓慧,孙宗喜,苏瑞强,等.柴胡及其活性成分药理研究进展.中国中医药信息杂志,2012,19(12):105-107.
5王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展.中华中医药学刊,2015,33(1):206-211.
6潘燕,孙立弟.柴胡-黄芩药对配伍前后有效成分比较研究.大连大学学报,2016,37(6):78-81.
7黄艳萍,刘浩.黄芩与柴胡不同比例配伍黄芩苷水煎出量的比较.海峡药学,2007,19(10):59-61.
8刘逢芹,刘田云,余晓东,等.RP-HPLC考察黄芩、柴胡配伍对黄芩苷成分的影响.中国实验方剂学杂志,2009,15(7):3-5.
9刘逢芹,常跃兴,刘田云,等.不同溶剂对黄芩-柴胡药对中的药效成分提出的影响.中药材,2009,32(6):900-903.
10侯绪浩,刘逢芹,杨晓静,等.实验考察柴胡与黄芩配伍前后柴胡皂苷a、d的溶出性差异.齐鲁药事,2012,31(2):66-67,74.
11江山,尤世刚,占国清,等.小柴胡汤治疗慢性乙型肝炎肝纤维化40例.实用医学杂志,2007,23(19):3105-3106.
12王宪龄,刘仁慧,张影,等.柴胡黄芩配伍抗小鼠急性酒精性肝损伤的实验研究.中药材,2004,27(10):756-758.
13王宪龄,李连珍,牛万强.柴胡与黄芩不同剂量比例配伍对扑热息痛所致小鼠急性肝损伤的保护作用.河南中医,2004,24(8):15-17.
14王宪龄,李伟,刘方洲,等.柴胡黄芩配伍对CCl4所致小鼠急性肝损伤的研究.中药药理与临床,2005,21(6):11-13.
15王宪龄,张丽萍,李连珍,等.柴胡黄芩配伍不同提取部位对扑热息痛所致小鼠急性肝损伤的保护作用.中药药理与临床,2005,21(3):10-11.
16王宪龄,李瑞琴,吴磊.柴胡黄芩配伍不同提取部位对小鼠急性酒精性肝损伤的作用.中药药理与临床,2007,23(1):10-12.
17陈慧慧.药对配伍对柴胡-黄芩主要药效作用影响的研究.济南:山东中医药大学硕士研究生学位论文,2011.
18唐永翠,严敏,孙付军,等.柴胡、黄芩含药血清对异烟肼致肝细胞HepG2损伤的预防作用.山东医药,2014,54(35):35-37.
19 Wang Q,Zheng X L,Yang L,et al.Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells.J Exp Clin Cancer Res,2010,29(1):159.
20 Wang H F,Zhang Y L,Bai R X,et al.Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress,inflammation and sonic hedgehog pathway in Rats.Cell Physiol Bioche,2016,3(39):1129-1140.
21王宪龄,程玲,宋宁,等.柴胡黄芩及两者不同比例配伍抗大鼠CCl4慢性肝损伤作用的比较研究.中药药理与临床,2009,25(6):70-73.
22 Nanji A,Miao L,Thomas P,et al.Enhanced cyclooxygenase-2 gene expression in alcoholic liver disease in the rat.Gastroenterology,1997,112(3):943-951.
23 Enomoto N,Ikejima K,Yamashina S,et al.Kupffer cell-derived prostaglandin E2 is involved in alcohol-induced fat accumulation in rat liver.Am J Physiol-gastr L,2000,279(1):100-106.
24王宪龄,宋宁.柴胡黄芩配伍不同提取部位对大鼠急性酒精性肝损伤肝组织中环氧合酶-2表达的影响.中药材,2006,29(9):943-945.
25仝艳,李晓飞,王宪龄.柴胡-黄芩药对主要成分与环氧化酶-2的相互作用.中国实验方剂学杂志,2014,20(15):99-103.
26 Seki E,Brenner D A.Toll-like receptors and adaptor molecules in liver disease:update.Hepatology,2008,48(1):322-335.
27李小菲,李静,王義雯,等.柴胡、柴胡和黄芩配伍对肝细胞损伤体外作用.吉林中医药,2016,36(2):176-180.
28李敏,王羲雯,李小菲,等.基于TLR4-NFκB的柴胡皂苷d-黄芩苷配伍抗CCl4损伤肝细胞的作用研究.中医药导报,2017,23(18):26-29.
29 Xu Y,Feng Y,Li H,et al.Ferric citrate cyp2e1-independently promotes alcohol-induced apoptosis in hepg2 cells via oxidative/nitrative stress which is attenuated by pretreatment with baicalin.Food Chem Toxicol,2012,50(9):3264-3272.
30 Chen C H,Chen M F,Huang S J,et al.Saikosaponin a induces apoptosis through mitochondria-dependent pathway in hepatic stellate cells.Am J Chinese Med,2017,45(2):1-18.
31王斌,李敏,侯建平.基于TLR4-NFκB信号通路的柴胡-黄芩药对抗肝纤维研究.中药药理与临床,2015,31(3):103-106.
32李敏,韩艳,王华林,等.基于TLR4-NF-κB的柴胡黄芩水煎液抑制CCl4诱导大鼠肝星状细胞激活作用机制研究.中国药理学通报,2017,33(5):729-732.
33李敏,高凯,王华林,等.柴胡皂苷d-黄芩苷配伍对CCl4诱导大鼠HSC的TLR4-NF-κB通路作用研究.陕西中医,2018,39(1):3-5.
34王雪峰,周丽.柴胡黄芩提取物对CVB3m感染小鼠心肌损伤影响的实验研究.辽宁中医杂志,2004,31(11):890-891.
35刘芳,王雪峰,南春红,等.黄芩、柴胡对CVB3m小鼠心肌炎凋亡及相关基因Bcl-2/Bax表达的影响.中药药理与临床,2010,26(6):53-56.
36刘芳,王雪峰,闫丽娟,等.黄芩柴胡及其配伍提取物对心肌炎小鼠心肌组织CVB3m RNA复制及IFNm RNA表达的影响.中华中医药学刊,2010,28(8):1601-1604.
37谢彬,王雪峰,闫丽娟.柴胡、黄芩及其配伍提取物含药血清对CVB3m感染乳鼠心肌细胞的保护作用研究.辽宁中医杂志,2011,38(6):1214-1216.
38阿荣高娃.柴胡与黄芩伍用的抗病毒作用.内蒙古中医药,1999,18(S1):98.
39 Qin H F,Wang Z X,Cai P P,et al.Traditional chinese medicine and related active compounds:A review of their Role on hepatitis B virus infection.Drug Discoveries&Therapeutics,2013,7(6):212-224.
40丁莹月.柴黄颗粒治疗小儿急性上呼吸道感染发热120例临床观察.现代医药卫生,2011,27(7):985.
41陈慧慧,张敏,虞慧娟,等.柴胡和黄芩配伍解热抗炎作用研究.中成药,2011,33(9):1596-1598.
42高琳,白晶,刘迪谦.柴胡-黄芩水煎液中不同化学成分群配伍与其解热作用相关性研究.北京中医药大学学报,2006,29(11):760-764.
43杨江萍.柴胡-黄芩药对退热有效部位及其配伍作用机制的研究.北京:北京中医药大学博士研究生学位论文,2012.
44阙任烨,李勇.柴胡皂苷防治原发性肝癌机制研究进展.辽宁中医药大学学报,2014,16(2):128-131.
45向东,杨金玉,张程亮,等.黄芩苷治疗原发性肝癌的研究进展.世界华人消化杂志,2018,26(6):378-383.
46杨秀秀.柴胡皂苷A、黄芩素及其配伍对人肝癌细胞HepG2和SMMC-7721体外作用的实验研究.郑州:河南中医学院硕士研究生学位论文,2014.
47王宪龄,周志.试探柴胡黄芩药对清泄肝胆的配伍作用.中医研究,2009,22(6):6-9.
48孙付军,陈慧慧,王春芳,等.柴胡、黄芩药对利胆作用研究.中成药,2011,33(8):1418-1420.
49王伟.难治性癫痫从肝论治体会.四川中医,2000,18(12):7-8.
50谢炜,梁一超,陈伟军,等.柴胡黄芩配伍应用对小鼠急性癫痫模型的影响.世界科学技术-中医药现代化,2013,15(2):210-214.
2张智华.《伤寒论》柴胡药对探析.湖北中医杂志,2014,36(1):47-48.
3吕富银.黄芩常用药对浅述.世界中医药,2010,5(5):353-354.
4吕晓慧,孙宗喜,苏瑞强,等.柴胡及其活性成分药理研究进展.中国中医药信息杂志,2012,19(12):105-107.
5王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展.中华中医药学刊,2015,33(1):206-211.
6潘燕,孙立弟.柴胡-黄芩药对配伍前后有效成分比较研究.大连大学学报,2016,37(6):78-81.
7黄艳萍,刘浩.黄芩与柴胡不同比例配伍黄芩苷水煎出量的比较.海峡药学,2007,19(10):59-61.
8刘逢芹,刘田云,余晓东,等.RP-HPLC考察黄芩、柴胡配伍对黄芩苷成分的影响.中国实验方剂学杂志,2009,15(7):3-5.
9刘逢芹,常跃兴,刘田云,等.不同溶剂对黄芩-柴胡药对中的药效成分提出的影响.中药材,2009,32(6):900-903.
10侯绪浩,刘逢芹,杨晓静,等.实验考察柴胡与黄芩配伍前后柴胡皂苷a、d的溶出性差异.齐鲁药事,2012,31(2):66-67,74.
11江山,尤世刚,占国清,等.小柴胡汤治疗慢性乙型肝炎肝纤维化40例.实用医学杂志,2007,23(19):3105-3106.
12王宪龄,刘仁慧,张影,等.柴胡黄芩配伍抗小鼠急性酒精性肝损伤的实验研究.中药材,2004,27(10):756-758.
13王宪龄,李连珍,牛万强.柴胡与黄芩不同剂量比例配伍对扑热息痛所致小鼠急性肝损伤的保护作用.河南中医,2004,24(8):15-17.
14王宪龄,李伟,刘方洲,等.柴胡黄芩配伍对CCl4所致小鼠急性肝损伤的研究.中药药理与临床,2005,21(6):11-13.
15王宪龄,张丽萍,李连珍,等.柴胡黄芩配伍不同提取部位对扑热息痛所致小鼠急性肝损伤的保护作用.中药药理与临床,2005,21(3):10-11.
16王宪龄,李瑞琴,吴磊.柴胡黄芩配伍不同提取部位对小鼠急性酒精性肝损伤的作用.中药药理与临床,2007,23(1):10-12.
17陈慧慧.药对配伍对柴胡-黄芩主要药效作用影响的研究.济南:山东中医药大学硕士研究生学位论文,2011.
18唐永翠,严敏,孙付军,等.柴胡、黄芩含药血清对异烟肼致肝细胞HepG2损伤的预防作用.山东医药,2014,54(35):35-37.
19 Wang Q,Zheng X L,Yang L,et al.Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells.J Exp Clin Cancer Res,2010,29(1):159.
20 Wang H F,Zhang Y L,Bai R X,et al.Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress,inflammation and sonic hedgehog pathway in Rats.Cell Physiol Bioche,2016,3(39):1129-1140.
21王宪龄,程玲,宋宁,等.柴胡黄芩及两者不同比例配伍抗大鼠CCl4慢性肝损伤作用的比较研究.中药药理与临床,2009,25(6):70-73.
22 Nanji A,Miao L,Thomas P,et al.Enhanced cyclooxygenase-2 gene expression in alcoholic liver disease in the rat.Gastroenterology,1997,112(3):943-951.
23 Enomoto N,Ikejima K,Yamashina S,et al.Kupffer cell-derived prostaglandin E2 is involved in alcohol-induced fat accumulation in rat liver.Am J Physiol-gastr L,2000,279(1):100-106.
24王宪龄,宋宁.柴胡黄芩配伍不同提取部位对大鼠急性酒精性肝损伤肝组织中环氧合酶-2表达的影响.中药材,2006,29(9):943-945.
25仝艳,李晓飞,王宪龄.柴胡-黄芩药对主要成分与环氧化酶-2的相互作用.中国实验方剂学杂志,2014,20(15):99-103.
26 Seki E,Brenner D A.Toll-like receptors and adaptor molecules in liver disease:update.Hepatology,2008,48(1):322-335.
27李小菲,李静,王義雯,等.柴胡、柴胡和黄芩配伍对肝细胞损伤体外作用.吉林中医药,2016,36(2):176-180.
28李敏,王羲雯,李小菲,等.基于TLR4-NFκB的柴胡皂苷d-黄芩苷配伍抗CCl4损伤肝细胞的作用研究.中医药导报,2017,23(18):26-29.
29 Xu Y,Feng Y,Li H,et al.Ferric citrate cyp2e1-independently promotes alcohol-induced apoptosis in hepg2 cells via oxidative/nitrative stress which is attenuated by pretreatment with baicalin.Food Chem Toxicol,2012,50(9):3264-3272.
30 Chen C H,Chen M F,Huang S J,et al.Saikosaponin a induces apoptosis through mitochondria-dependent pathway in hepatic stellate cells.Am J Chinese Med,2017,45(2):1-18.
31王斌,李敏,侯建平.基于TLR4-NFκB信号通路的柴胡-黄芩药对抗肝纤维研究.中药药理与临床,2015,31(3):103-106.
32李敏,韩艳,王华林,等.基于TLR4-NF-κB的柴胡黄芩水煎液抑制CCl4诱导大鼠肝星状细胞激活作用机制研究.中国药理学通报,2017,33(5):729-732.
33李敏,高凯,王华林,等.柴胡皂苷d-黄芩苷配伍对CCl4诱导大鼠HSC的TLR4-NF-κB通路作用研究.陕西中医,2018,39(1):3-5.
34王雪峰,周丽.柴胡黄芩提取物对CVB3m感染小鼠心肌损伤影响的实验研究.辽宁中医杂志,2004,31(11):890-891.
35刘芳,王雪峰,南春红,等.黄芩、柴胡对CVB3m小鼠心肌炎凋亡及相关基因Bcl-2/Bax表达的影响.中药药理与临床,2010,26(6):53-56.
36刘芳,王雪峰,闫丽娟,等.黄芩柴胡及其配伍提取物对心肌炎小鼠心肌组织CVB3m RNA复制及IFNm RNA表达的影响.中华中医药学刊,2010,28(8):1601-1604.
37谢彬,王雪峰,闫丽娟.柴胡、黄芩及其配伍提取物含药血清对CVB3m感染乳鼠心肌细胞的保护作用研究.辽宁中医杂志,2011,38(6):1214-1216.
38阿荣高娃.柴胡与黄芩伍用的抗病毒作用.内蒙古中医药,1999,18(S1):98.
39 Qin H F,Wang Z X,Cai P P,et al.Traditional chinese medicine and related active compounds:A review of their Role on hepatitis B virus infection.Drug Discoveries&Therapeutics,2013,7(6):212-224.
40丁莹月.柴黄颗粒治疗小儿急性上呼吸道感染发热120例临床观察.现代医药卫生,2011,27(7):985.
41陈慧慧,张敏,虞慧娟,等.柴胡和黄芩配伍解热抗炎作用研究.中成药,2011,33(9):1596-1598.
42高琳,白晶,刘迪谦.柴胡-黄芩水煎液中不同化学成分群配伍与其解热作用相关性研究.北京中医药大学学报,2006,29(11):760-764.
43杨江萍.柴胡-黄芩药对退热有效部位及其配伍作用机制的研究.北京:北京中医药大学博士研究生学位论文,2012.
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