艾灸“神阙”“足三里”穴对痴呆大鼠脂联素及受体表达的影响
|
摘要
目的:通过比较艾灸不同腧穴对阿尔茨海默病(AD)大鼠血清及脂肪组织中脂联素及脂联素受体的影响,探讨艾灸"神阙""足三里"对AD大鼠脂质代谢的作用机制。方法:SD大鼠随机分为空白组、模型组、神阙组、足三里组、神阙-足三里组,每组10只。大鼠腹腔注射D-半乳糖、氢溴酸东莨菪碱复制AD模型,各治疗组艾灸相应腧穴,每日治疗1次,每次每穴艾灸3壮,连续5周。用Morris水迷宫定位航行实验检测大鼠记忆能力,用酶联免疫吸附法检测大鼠血清中脂联素的水平,用荧光定量PCR和Western blot法分别检测大鼠脂肪组织中脂联素mRNA及脂联素受体的表达。结果:与正常组比较,模型组大鼠首次水迷宫定位航行实验的逃避潜伏期显著延长,说明造模成功。干预结束后,与正常组比较,模型组大鼠血清中脂联素含量显著降低(P<0.01),脂肪组织中脂联素mRNA显著降低(P<0.01),脂肪组织中脂联素受体表达显著下降(P<0.01)。与模型组比较,各艾灸组大鼠逃避潜伏期显著缩短(P<0.05),血清中脂联素含量明显升高(P<0.01),脂肪组织中脂联素mRNA表达明显增多(P<0.05),脂肪组织中脂联素受体表达明显升高(P<0.01),。各治疗组之间差异无统计学意义(P>0.05)。结论:艾灸"神阙""足三里"穴或两穴合用都能提高AD大鼠血清及脂肪组织中脂联素及脂联素受体水平,进而改善AD的学习记忆能力。
Objective To observe the effect of moxibustion on serum adiponectin content,and expression of adiponectin and adiponectin receptor in adipose tissue in Alzheimer's disease(AD)rats,so as to explore its mechanism underlying improvement of AD.Methods Fifty male SD rats were randomly divided into control,model,Shenque(CV8),Zusanli(ST36)and CV8+ST36 groups(n=10 in each group).The AD model was established by intraperitoneal injection of D-galactose(400 mg·kg-1·d-1)for 5 weeks and scopolamine hydrobromide(3 mg·kg-1·d-1)for 2 weeks.Moxibustion was applied to CV8,ST36 and CV8+ST36 respectively for 3 moxa-cones every time,once daily for 5 weeks.Morris water maze tests were used to assess the rats' learning-memory ability.The contents of serum adiponectin were assayed using ELISA,and the expression of adiponectin and adiponectin receptor in the adipose tissue was detected by quantitative real-time PCR and Western blot,separately.Results Following modeling,the average escape latency of Morris water maze tests was significantly prolonged(P<0.05),the content of serum adiponectin and the expression level of adiponectin mRNA in adipose tissue were significantly decreased(P<0.01),the expression of adiponectin receptor protein significantly decreased in the model group relevant to the control group(P<0.01).After the intervention,the average escape latency was significantly shortened(P<0.05),the decreased serum adiponectin content and adiponectin mRNA expression,and the decreased adiponectin receptor protein expression in adipose tissue were all reversed in the 3 treatment groups(P<0.01,P<0.05).No significant differences were found among the three moxibustion groups in the above indexes(P>0.05).Conclusion Moxibustion at CV8,ST36 and CV8+ST36 is effective in up-regulating serum adiponectin content,adiponectin mRNA expression and adiponectin receptor protein expression in adipose tissue,which may provide evidence for clinical election of acupoints.
Objective To observe the effect of moxibustion on serum adiponectin content,and expression of adiponectin and adiponectin receptor in adipose tissue in Alzheimer's disease(AD)rats,so as to explore its mechanism underlying improvement of AD.Methods Fifty male SD rats were randomly divided into control,model,Shenque(CV8),Zusanli(ST36)and CV8+ST36 groups(n=10 in each group).The AD model was established by intraperitoneal injection of D-galactose(400 mg·kg-1·d-1)for 5 weeks and scopolamine hydrobromide(3 mg·kg-1·d-1)for 2 weeks.Moxibustion was applied to CV8,ST36 and CV8+ST36 respectively for 3 moxa-cones every time,once daily for 5 weeks.Morris water maze tests were used to assess the rats' learning-memory ability.The contents of serum adiponectin were assayed using ELISA,and the expression of adiponectin and adiponectin receptor in the adipose tissue was detected by quantitative real-time PCR and Western blot,separately.Results Following modeling,the average escape latency of Morris water maze tests was significantly prolonged(P<0.05),the content of serum adiponectin and the expression level of adiponectin mRNA in adipose tissue were significantly decreased(P<0.01),the expression of adiponectin receptor protein significantly decreased in the model group relevant to the control group(P<0.01).After the intervention,the average escape latency was significantly shortened(P<0.05),the decreased serum adiponectin content and adiponectin mRNA expression,and the decreased adiponectin receptor protein expression in adipose tissue were all reversed in the 3 treatment groups(P<0.01,P<0.05).No significant differences were found among the three moxibustion groups in the above indexes(P>0.05).Conclusion Moxibustion at CV8,ST36 and CV8+ST36 is effective in up-regulating serum adiponectin content,adiponectin mRNA expression and adiponectin receptor protein expression in adipose tissue,which may provide evidence for clinical election of acupoints.
引文
[1]朱晶,国海东,邵水金.针灸治疗阿尔茨海默病机制的研究进展[J].针刺研究,2012,37(5):422-427.
[2]韩亚,宋荣蓉.脂联素与血管性痴呆危险因素关系的研究进展[J].医学综述,2016,22(12):2313-2316.
[3]VAN ECHTEN-DECKERT G,WALTER J.Sphingolipids critical players in Alzheimer’s disease[J].Prog Lipid Res,2012,51(4):378-393.
[4]姜国良,于晓,徐恺,等.腹腔和皮下注射D-半乳糖衰老大鼠模型分析[J].中国老年学杂志,2013,33(5):1101-1103.
[5]肖移生,曾元凤,欧阳厚淦,等.黄精地龙方对老年痴呆大鼠行为认知及脑胆碱能系统的影响[J].中药药理与临床,2013,29(4):146-148.
[6]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:327.
[7]王海军,冀来喜.关于大鼠“神阙”穴的定位[J].针刺研究,2007,32(5):312.
[8]罗小泉,骆利平,陈海芳,等.Morris水迷宫检测大鼠记忆力方法的探讨[J].时珍国医国药,2010,21(10):2667-2669.
[9]封敏,卢圣锋,张承舜,等.国内大鼠Morris水迷宫实验现状与分析[J].辽宁中医杂志,2011,38(11):2170-2172.
[10]石学敏.针灸学[M].北京:中国中医药出版社,2006:230.
[11]吴炳煌,林宏,阙庆辉,等.隔姜灸神阙治疗慢性腹泻疗效与免疫功能的关系[J].福建中医学院学报,1997,7(3):20-21.
[12]谭杏,杨茜芸,林亚平.艾灸足三里穴对衰老大鼠学习记忆及抗疲劳能力的影响[J].中国老年学杂志,2015,12(35):6976-6978.
[13]赵彩娇,李娴,覃永贞,等.艾灸足三里对衰老模型豚鼠血清SOD及肝肾组织基因表达的影响[J].时珍国医国药,2016,27(10):2543-2545.
[14]王凤玲,李蕙,魏正岫,等.灸神阙穴对中老年人免疫功能及其全身状态的影响[J].中国针灸,1996,16(7):39-40.
[15]唐英,马彩云,尚清,等.艾灸对脑瘫患儿免疫功能的影响[J].中国针灸,2016,36(1):12-16.
[16]王刚,任汝静.阿尔茨海默病及其他类型痴呆的临床实践指南[M].上海:上海交通大学出版社,2015:1.
[17]杨裕华,王际莘,鲁燕.糖脂代谢对老年痴呆影响的研究进展[J].广东医学,2012,33(18):2849-2853.
[18]FISMAN E Z,TENENBAUM A.Adiponectin:a manifold therapeutic target for metabolic syndrome,diabetes,and coronary disease[J].Cardiovasc Diabetol,2014,13:103.
[19]KAMOGAWA K,KOHARA K,TABARA Y,et al.Abdominal fat,adiposederived hormones and mild cognitive impairment:the J-SHIPP study[J].Dement Geriatr Cogn Disord,2010,30(5):432-439.
[20]DUARTE A I,MOREIRA P I,OLIVEIRA C R.Insulin in central nervous system:more than just a peripheral hormone[J].JAging Res,2012:384017.
[21]沈琳辉,缪婕,赵雅洁,等.脂联素受体在短暂性脑缺血小鼠模型脑组织中的表达[J].中华老年心脑血管病杂志,2014,16(10):1086-1087.
[22]HOLMES C,BUTCHART J.Systemic inflammation and Alzheimer’s disease[J].Biochem Soc Trans,2011,39(4):898-901.
[2]韩亚,宋荣蓉.脂联素与血管性痴呆危险因素关系的研究进展[J].医学综述,2016,22(12):2313-2316.
[3]VAN ECHTEN-DECKERT G,WALTER J.Sphingolipids critical players in Alzheimer’s disease[J].Prog Lipid Res,2012,51(4):378-393.
[4]姜国良,于晓,徐恺,等.腹腔和皮下注射D-半乳糖衰老大鼠模型分析[J].中国老年学杂志,2013,33(5):1101-1103.
[5]肖移生,曾元凤,欧阳厚淦,等.黄精地龙方对老年痴呆大鼠行为认知及脑胆碱能系统的影响[J].中药药理与临床,2013,29(4):146-148.
[6]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:327.
[7]王海军,冀来喜.关于大鼠“神阙”穴的定位[J].针刺研究,2007,32(5):312.
[8]罗小泉,骆利平,陈海芳,等.Morris水迷宫检测大鼠记忆力方法的探讨[J].时珍国医国药,2010,21(10):2667-2669.
[9]封敏,卢圣锋,张承舜,等.国内大鼠Morris水迷宫实验现状与分析[J].辽宁中医杂志,2011,38(11):2170-2172.
[10]石学敏.针灸学[M].北京:中国中医药出版社,2006:230.
[11]吴炳煌,林宏,阙庆辉,等.隔姜灸神阙治疗慢性腹泻疗效与免疫功能的关系[J].福建中医学院学报,1997,7(3):20-21.
[12]谭杏,杨茜芸,林亚平.艾灸足三里穴对衰老大鼠学习记忆及抗疲劳能力的影响[J].中国老年学杂志,2015,12(35):6976-6978.
[13]赵彩娇,李娴,覃永贞,等.艾灸足三里对衰老模型豚鼠血清SOD及肝肾组织基因表达的影响[J].时珍国医国药,2016,27(10):2543-2545.
[14]王凤玲,李蕙,魏正岫,等.灸神阙穴对中老年人免疫功能及其全身状态的影响[J].中国针灸,1996,16(7):39-40.
[15]唐英,马彩云,尚清,等.艾灸对脑瘫患儿免疫功能的影响[J].中国针灸,2016,36(1):12-16.
[16]王刚,任汝静.阿尔茨海默病及其他类型痴呆的临床实践指南[M].上海:上海交通大学出版社,2015:1.
[17]杨裕华,王际莘,鲁燕.糖脂代谢对老年痴呆影响的研究进展[J].广东医学,2012,33(18):2849-2853.
[18]FISMAN E Z,TENENBAUM A.Adiponectin:a manifold therapeutic target for metabolic syndrome,diabetes,and coronary disease[J].Cardiovasc Diabetol,2014,13:103.
[19]KAMOGAWA K,KOHARA K,TABARA Y,et al.Abdominal fat,adiposederived hormones and mild cognitive impairment:the J-SHIPP study[J].Dement Geriatr Cogn Disord,2010,30(5):432-439.
[20]DUARTE A I,MOREIRA P I,OLIVEIRA C R.Insulin in central nervous system:more than just a peripheral hormone[J].JAging Res,2012:384017.
[21]沈琳辉,缪婕,赵雅洁,等.脂联素受体在短暂性脑缺血小鼠模型脑组织中的表达[J].中华老年心脑血管病杂志,2014,16(10):1086-1087.
[22]HOLMES C,BUTCHART J.Systemic inflammation and Alzheimer’s disease[J].Biochem Soc Trans,2011,39(4):898-901.