力学因素对骨髓间充质干细胞成骨分化的影响
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摘要
近年来,组织工程学领域发展突飞猛进,已被列为一种修复或再生许多组织和器官的方法。骨髓间充质干细胞具有良好的成骨向分化潜能,在骨组织工程领域中具有广阔的应用前景。骨髓间充质干细胞增殖和成骨向分化受多种力学因素的影响,且不同性质的力学刺激对其定向分化的调节作用不尽相同。目前,许多学者致力于深入探讨力学因素影响骨髓间充质干细胞成骨向分化的具体途径,但其调控机制尚未完全明确。本文综述及讨论力学因素对骨髓间充质干细胞所产生的增殖、定向成骨分化等生物学效应的影响及可能涉及的力化学信号转导通路作用机制,以期丰富研究思路和理论依据。
In recent years, the field of tissue engineering has grown by leaps and bounds and has been classified as a method of repairing or regenerating many tissues and organs. Bone marrow mesenchymal stem cells have good osteogenic differentiation potential and have broad application prospects in the field of bone tissue engineering. Bone marrow mesenchymal stem cell proliferation and osteogenic differentiation are affected by a variety of mechanical factors, and the mechanical stimulation of different properties has different regulatory effects on directional differentiation. At present, many scholars are devoted to in-depth study of the specific ways in which mechanical factors affect the osteogenic differentiation of bone marrow mesenchymal stem cells, but their regulatory mechanisms have not yet been fully defined. This article reviews and discusses the effects of mechanical factors on the biological effects of bone marrow mesenchymal stem cells, such as proliferation and directed osteogenic differentiation, and the possible mechanisms of the force chemical signal transduction pathway, in order to enrich the research ideas and theoretical basis.
In recent years, the field of tissue engineering has grown by leaps and bounds and has been classified as a method of repairing or regenerating many tissues and organs. Bone marrow mesenchymal stem cells have good osteogenic differentiation potential and have broad application prospects in the field of bone tissue engineering. Bone marrow mesenchymal stem cell proliferation and osteogenic differentiation are affected by a variety of mechanical factors, and the mechanical stimulation of different properties has different regulatory effects on directional differentiation. At present, many scholars are devoted to in-depth study of the specific ways in which mechanical factors affect the osteogenic differentiation of bone marrow mesenchymal stem cells, but their regulatory mechanisms have not yet been fully defined. This article reviews and discusses the effects of mechanical factors on the biological effects of bone marrow mesenchymal stem cells, such as proliferation and directed osteogenic differentiation, and the possible mechanisms of the force chemical signal transduction pathway, in order to enrich the research ideas and theoretical basis.
引文
[1]Huang C,Ogawa R.Effect of hydrostatic pressure on bone regeneration using human mesenchymal stem cells[J].Tissue Eng Part A,2012,18(19-20):2106-2113.
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[3]姜喜亮,朱晓文,胡静,等.整合素信号通路在大鼠骨髓间充质干细胞牵张中的作用和转导机制[J].中国口腔颌面外科杂志,2015,13(3):193-199.
[4]Liu J,Zhao Z,Zou L,et al.Pressure-Loaded MSCs During Early Osteodifferentiation Promote Osteoclastogenesis by Iincrease of RANKL/OPG Ratio[J].Annals of Biomedical Engineering,2009,37(4):794-802.
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[7]代庆刚,张鹏,吴玉琼.不同幅度持续张应力干预下骨髓基质干细胞的成骨分化[J].中国组织工程研究,2012,16(45):8367-8373.
[8]张鹏,江凌勇,吴玉琼.持续张应力对骨髓基质干细胞增殖及骨向分化的影响[J].医用生物力学,2012,27(2):239-244.
[9]Song Y,Tang Y,Song J,et al.Cyclic mechanical stretch enhances BMP9-induced osteogenic differentiation of mesenchymal stem cells[J].Int Orthop,2018,42(4):947-955.
[10]Liu L,Yu B,Chen J,et al.Different effects of intermittent and continuous fluid shear stresses on osteogenic differentiationof human mesenchymal stem cells[J].Biomech Model Mechanobiol,2012,11(3-4):391-401.
[11]Vetsch JR,Betts DC,Mu¨ller R,et al.Flow velocity-driven differentiation of human mesenchymal stromal cells in silk fibroin scaffolds:A combined experimental and computational approach[J].PLoS One,2017,12(7):e0180781.
[12]Hu K,Sun H,Gui B,et al.TRPV4 functions in flow shear stress induced early osteogenic differentiation of human bone marrow mesenchymal stem cells[J].Biomed Pharmacother.2017(91):841-848.
[13]Becquart P,Cruel M,Hoc T,et al.Human mesenchymal stem cell responses to hydrostatic pressure and shear stress[J].European Cells and Materials,2016(31):160-173.
[14].李煜,孙明林,张新昌,等.微重力对骨髓间充质干细胞向成骨细胞分化影响的研究[J].国际生物医学工程杂志,2015,38(5):257-261.
[15]Mao XL,Chen Z,Luo Q,et al.Simulated microgravity inhibits the migration of mesenchymal stem cells by remodeling actin cytoskeleton and increasing cell stiffness[J].Cytotechnology,2016,68(6):2235-2243.
[16]Li L,Zhang C,Chen JL,et al.Effects of simulated microgravity on the expression profiles of RNA during osteogenic differentiation of human bone marrow mesenchymal stem cells[J].Cell Prolif,2018(5):e12539.
[17]杨先炯,毛新建,罗庆,等.Wnt3a/β-catenin信号通路调节模拟微重力诱导的骨髓间充质干细胞增殖抑制[J].空间科学学报,2016,36(1):63-70.
[18]蒋斌,郑明辉,张斌,等.三维载体培养下离心力对骨髓间充质干细胞增殖的影响[J].解剖学杂志,2018,41(1):1-4.
[19]段峰,关键,杨红岩,等.机械离心力对成骨细胞骨形态发生蛋白信号通路中Runx-2 m RNA的影响[J].中国组织工程研究,2014,18(33):5305-5309.
[2]Stavenschi E,Corrigan MA,Johnson GP,et al.Physiological cyclic hydrostatic pressure induces osteogenic lineage commitment of human bone marrow stem cells:a systematic study.Stavenschi et al[J].Stem Cell Res Ther,2018,9(1):276.
[3]姜喜亮,朱晓文,胡静,等.整合素信号通路在大鼠骨髓间充质干细胞牵张中的作用和转导机制[J].中国口腔颌面外科杂志,2015,13(3):193-199.
[4]Liu J,Zhao Z,Zou L,et al.Pressure-Loaded MSCs During Early Osteodifferentiation Promote Osteoclastogenesis by Iincrease of RANKL/OPG Ratio[J].Annals of Biomedical Engineering,2009,37(4):794-802.
[5]Yu SH,Kim JJ,Kim HW,et al.Impact of mechanical stretch on the cell behaviors of bone and surrounding tissues[J].J Tissue Eng,2016(7):1-24.
[6]Grier WG,Moy AS,Harley BA.Harley.Cyclic tensile strain enhances human mesenchymal stem cell smad 2/3 activation and tenogenic differentiation in anisotropic collagen-glycosaminoglycan scaffolds[J].Eur Cell Mater,2018(33):227-239.
[7]代庆刚,张鹏,吴玉琼.不同幅度持续张应力干预下骨髓基质干细胞的成骨分化[J].中国组织工程研究,2012,16(45):8367-8373.
[8]张鹏,江凌勇,吴玉琼.持续张应力对骨髓基质干细胞增殖及骨向分化的影响[J].医用生物力学,2012,27(2):239-244.
[9]Song Y,Tang Y,Song J,et al.Cyclic mechanical stretch enhances BMP9-induced osteogenic differentiation of mesenchymal stem cells[J].Int Orthop,2018,42(4):947-955.
[10]Liu L,Yu B,Chen J,et al.Different effects of intermittent and continuous fluid shear stresses on osteogenic differentiationof human mesenchymal stem cells[J].Biomech Model Mechanobiol,2012,11(3-4):391-401.
[11]Vetsch JR,Betts DC,Mu¨ller R,et al.Flow velocity-driven differentiation of human mesenchymal stromal cells in silk fibroin scaffolds:A combined experimental and computational approach[J].PLoS One,2017,12(7):e0180781.
[12]Hu K,Sun H,Gui B,et al.TRPV4 functions in flow shear stress induced early osteogenic differentiation of human bone marrow mesenchymal stem cells[J].Biomed Pharmacother.2017(91):841-848.
[13]Becquart P,Cruel M,Hoc T,et al.Human mesenchymal stem cell responses to hydrostatic pressure and shear stress[J].European Cells and Materials,2016(31):160-173.
[14].李煜,孙明林,张新昌,等.微重力对骨髓间充质干细胞向成骨细胞分化影响的研究[J].国际生物医学工程杂志,2015,38(5):257-261.
[15]Mao XL,Chen Z,Luo Q,et al.Simulated microgravity inhibits the migration of mesenchymal stem cells by remodeling actin cytoskeleton and increasing cell stiffness[J].Cytotechnology,2016,68(6):2235-2243.
[16]Li L,Zhang C,Chen JL,et al.Effects of simulated microgravity on the expression profiles of RNA during osteogenic differentiation of human bone marrow mesenchymal stem cells[J].Cell Prolif,2018(5):e12539.
[17]杨先炯,毛新建,罗庆,等.Wnt3a/β-catenin信号通路调节模拟微重力诱导的骨髓间充质干细胞增殖抑制[J].空间科学学报,2016,36(1):63-70.
[18]蒋斌,郑明辉,张斌,等.三维载体培养下离心力对骨髓间充质干细胞增殖的影响[J].解剖学杂志,2018,41(1):1-4.
[19]段峰,关键,杨红岩,等.机械离心力对成骨细胞骨形态发生蛋白信号通路中Runx-2 m RNA的影响[J].中国组织工程研究,2014,18(33):5305-5309.