重离子束(C)辐照诱变东北粳稻后代变异的初步研究
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摘要
利用高能重离子束(C)辐照诱变东北粳稻通禾899,剂量为0(CK)、200Gy,初步研究了重离子束(C)辐照诱变后代材料出现的突变情况。结果表明,辐照M_1代全部为半不育,结实率5.2%;M_2代突变率5.7%,高于传统的γ、X射线辐照诱变的突变率,而且突变体的类型丰富;经过M_3～M_5代的连续种植和选择,后代突变体群体越来越大,大部分突变材料均已稳定,少量后代材料综合性状优良,生育期比对照通禾899明显提前,已参加吉林省水稻新品种联合区域试验。但有近30%的材料在M_5代还存在分离或疯狂分离,分离的材料绝大部分表现为长粒型,千粒重偏低,外观品质好。
A preliminary study on mutations of Tonghe 899, a japonica rice variety in Northeast China, was conducted after the heavy ion beam C irradiation on rice seeds with the dosages of Gy at 0, 200. The results showed that the generations of M_1 were all half sterile with seed setting rate of 5.2%. The mutant types were found various and mutant rate of M_2 maintained 5.7%, which was higher than the traditionally mutant rate in radiation with γ and X ray. Moreover, the mutant population showed more abundant after planting from M_3 to M_5. Characters of most mutants maintained stable. Some mutants were found excellent with more early growth period than Tonghe 899(CK)and have been conduct with the joint area test of new rice varieties in Jilin province. However, there are also 30%mutants showed apparent separates with the characters of long-grain, low 1000-grain weight and high appearance quality.
A preliminary study on mutations of Tonghe 899, a japonica rice variety in Northeast China, was conducted after the heavy ion beam C irradiation on rice seeds with the dosages of Gy at 0, 200. The results showed that the generations of M_1 were all half sterile with seed setting rate of 5.2%. The mutant types were found various and mutant rate of M_2 maintained 5.7%, which was higher than the traditionally mutant rate in radiation with γ and X ray. Moreover, the mutant population showed more abundant after planting from M_3 to M_5. Characters of most mutants maintained stable. Some mutants were found excellent with more early growth period than Tonghe 899(CK)and have been conduct with the joint area test of new rice varieties in Jilin province. However, there are also 30%mutants showed apparent separates with the characters of long-grain, low 1000-grain weight and high appearance quality.
引文
[1] HAYASHI Y, TAKEHISA H, KAZAMA Y, et al. Effects of ion beam irradiation on mutation induction in rice[J]. Cyclotr Their Appl,2007, 18:237-239.
[2] OKAMURA M, YASUNO N, OHTSUKA M, et al. Wide variety of flower-color and-shape mutants regenerated from leaf cultures irradiated with ion beams[J]. Nuclear Instruments and Methods in Physics Research Section B:Beam Interactions with Materials and Atoms, 2003, 206:574-578.
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[4] YAMAGUCHI H, HASE Y, TANAKA A, et al. Mutagenic effects of ion beam irradiation on rice[J]. Breed Sci, 2009, 59(2):169-177.
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[6]董喜存,李文建.碳离子束辐照对甜高粱节间参数的影响[J].辐射研究与辐射工艺学报,2008,26(5):314-316.
[7]贾蓉,苏锋涛,胡步荣.重离子的辐射生物效应及其在生命科学中的应用[J].生物技术通报,2017,34(4):1-6.
[8]卫增泉,颉红梅,梁剑平,等.重离子束在诱变育种和分子改造中的应用[J].原子能物理评论,2003,20(1):39.
[9] LI Q, WEI Z Q, LI W J. Calculation of depth-dose distribution of intermediate energy heavy-ion beams[J]. Chinese Science Bulletin,2002, 47(20):1 708-1 710.
[10]李仁民,王菊芳,李文建.重离子束在微生物诱变育种及生物能源开发中的应用[J].原子核物理评论,2007,24(3):234-237.
[11] GOODHEAD D T. Molecular and cell models of biological effects of heavy ion radiation[J]. Radiation and Environmental Biophysics,1995:34(2):67-72.
[12] SHIKAZONO N, SUZUKI C, KITAMURA S, et al. Analysis of mutations induced by carbon ions in Arabidopsis thaliana[J]. J Exp Bot,2005, 56(412):587-596.
[13] TANAKA A, HASE Y. Establishment of ion beam technology for breeding[M]//Shu Q Y. Induced plant mutations in the genomics era.Rome:Food and Agriculture Organization of the United Nations Press, 2009:243-246.
[14] LIEBER M R, MA Y M, PANNICKE U, et al. Mechanism and regulation of human non-homologous DNA end-joining[J]. Nat Rev Mol Cell Biol, 2003, 4(9):712-720.
[15] KARLSSON K H, STENERLOW B. Focus formation of DNA repair proteins in normal and repair-deficient cells irradiated with highLET ions[J]. Radiation Research, 2004:161(5):517-527.
[16]许雷.北方水稻遗传改良[M].北京:中国农业科学技术出版社,2015:192.
[2] OKAMURA M, YASUNO N, OHTSUKA M, et al. Wide variety of flower-color and-shape mutants regenerated from leaf cultures irradiated with ion beams[J]. Nuclear Instruments and Methods in Physics Research Section B:Beam Interactions with Materials and Atoms, 2003, 206:574-578.
[3] SHIKAZONO N, YOKOTA Y, KITAMURA S, et al. Mutation rate and novel tt mutants of Arabidopsis thaliana induced by carbon ions[J]. Genetics, 2003, 163(4):1 449-1 455.
[4] YAMAGUCHI H, HASE Y, TANAKA A, et al. Mutagenic effects of ion beam irradiation on rice[J]. Breed Sci, 2009, 59(2):169-177.
[5]赵连芝,王勇,甄东生,等.春小麦突变新品种“陇辐2号”[J].核农学报,2005,19(1):80.
[6]董喜存,李文建.碳离子束辐照对甜高粱节间参数的影响[J].辐射研究与辐射工艺学报,2008,26(5):314-316.
[7]贾蓉,苏锋涛,胡步荣.重离子的辐射生物效应及其在生命科学中的应用[J].生物技术通报,2017,34(4):1-6.
[8]卫增泉,颉红梅,梁剑平,等.重离子束在诱变育种和分子改造中的应用[J].原子能物理评论,2003,20(1):39.
[9] LI Q, WEI Z Q, LI W J. Calculation of depth-dose distribution of intermediate energy heavy-ion beams[J]. Chinese Science Bulletin,2002, 47(20):1 708-1 710.
[10]李仁民,王菊芳,李文建.重离子束在微生物诱变育种及生物能源开发中的应用[J].原子核物理评论,2007,24(3):234-237.
[11] GOODHEAD D T. Molecular and cell models of biological effects of heavy ion radiation[J]. Radiation and Environmental Biophysics,1995:34(2):67-72.
[12] SHIKAZONO N, SUZUKI C, KITAMURA S, et al. Analysis of mutations induced by carbon ions in Arabidopsis thaliana[J]. J Exp Bot,2005, 56(412):587-596.
[13] TANAKA A, HASE Y. Establishment of ion beam technology for breeding[M]//Shu Q Y. Induced plant mutations in the genomics era.Rome:Food and Agriculture Organization of the United Nations Press, 2009:243-246.
[14] LIEBER M R, MA Y M, PANNICKE U, et al. Mechanism and regulation of human non-homologous DNA end-joining[J]. Nat Rev Mol Cell Biol, 2003, 4(9):712-720.
[15] KARLSSON K H, STENERLOW B. Focus formation of DNA repair proteins in normal and repair-deficient cells irradiated with highLET ions[J]. Radiation Research, 2004:161(5):517-527.
[16]许雷.北方水稻遗传改良[M].北京:中国农业科学技术出版社,2015:192.