急性缺血性脑卒中患者T细胞亚群与NIHSS评分相关性研究
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摘要
目的探讨急性缺血性脑卒中患者的T细胞亚群与NIHSS评分的相关性。方法选取2017年1~10月高州市人民医院神经内科收治的160例确诊急性缺血性脑卒中患者为实验组,所有脑卒中患者入院当天按神经功能情况进行NIHSS评分,同时选取相对应的中老年健康体检者80例为对照组,对所有研究对象进行流式T细胞亚群分析,比较CD3~+、CD4~+、CD8~+和CD4~+/CD8~+水平在两组中的差异,分析各亚群与NIHSS评分的相关性。结果实验组患者的CD3~+、CD4~+和CD4~+/CD8~+均明显高于对照组,而CD8~+明显低于对照组,差异均有统计学意义(P<0.05);实验组患者的CD3~+、CD4~+和CD4~+/CD8~+与NIHSS评分呈显著正相关(r=0.295,0.498,0.887,P<0.05),而CD8~+与NIHSS评分呈显著负相关(r=-0.352,P<0.05)。结论 T细胞亚群与急性缺血性脑卒中NIHSS评分相关,与其疾病的发生和发展相关。
Objective To investigate the correlation between NIHSS score and T cell subsets in patients with acute ischemic stroke(AIS). Methods A total of 160 patients with AIS admitted to Department of Neurology, Gaozhou People's Hospital from January 2017 to October 2017 were selected as the experimental group. All stroke patients were evaluated for the National Institute of Health Stroke Scale(NIHSS) score on admission day according to the nerve function. At the same time, 80 healthy middle and old age persons were selected as the control group, and all subjects underwent flow cytometry. The differences of CD3~+, CD4~+, CD8~+and CD4~+/CD8~+levels between the two groups were compare, and the correlation between the subgroups and the NIHSS score were analyzed. Results The levels of CD3~+,CD4~+and CD4~+/CD8~+in experimental group were significantly higher than those in control group, but CD8~+was significantly lower than that of the control group(P<0.05). The CD3~+, CD4~+and CD4~+/CD8~+of the experimental group were positively correlated with the NIHSS score(r=0.295, r=0.498, r=0.887, P<0.05), but the CD8~+in the experimental group were negatively correlated with the NIHSS score(r=-0.352, P<0.05). Conclusion T cell subsets were associated with the NIHSS score of AIS, and it is related to the occurrence and development of the disease.
Objective To investigate the correlation between NIHSS score and T cell subsets in patients with acute ischemic stroke(AIS). Methods A total of 160 patients with AIS admitted to Department of Neurology, Gaozhou People's Hospital from January 2017 to October 2017 were selected as the experimental group. All stroke patients were evaluated for the National Institute of Health Stroke Scale(NIHSS) score on admission day according to the nerve function. At the same time, 80 healthy middle and old age persons were selected as the control group, and all subjects underwent flow cytometry. The differences of CD3~+, CD4~+, CD8~+and CD4~+/CD8~+levels between the two groups were compare, and the correlation between the subgroups and the NIHSS score were analyzed. Results The levels of CD3~+,CD4~+and CD4~+/CD8~+in experimental group were significantly higher than those in control group, but CD8~+was significantly lower than that of the control group(P<0.05). The CD3~+, CD4~+and CD4~+/CD8~+of the experimental group were positively correlated with the NIHSS score(r=0.295, r=0.498, r=0.887, P<0.05), but the CD8~+in the experimental group were negatively correlated with the NIHSS score(r=-0.352, P<0.05). Conclusion T cell subsets were associated with the NIHSS score of AIS, and it is related to the occurrence and development of the disease.
引文
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[2]Chen S,Wu H,Klebe D,et al.Regulatory T Cell in stroke:a new paradigm for immune regulation[J].Clin Dev Immunol,2013,2013:689827.
[3]徐姗姗,简志宏,熊晓星,等.T淋巴细胞在缺血性脑卒中的作用[J].国际神经病学神经外科学杂志,2015,42(5):467-470.
[4]谈颂,常思远,宋波,等.早期改良美国国立卫生研究院卒中量表评分对缺血性卒中预后的预测作用[J].中华神经科杂志,2012,45(3):154-157.
[5]中华医学会神经病学分会,中华医学会神经病学分会脑血管病学组.中国急性缺血性脑卒中诊治指南2014[S].中华神经科杂志,2015,48(4):246-257.
[6]孙德锦,曾庆宏,姜建东,等.脑梗死急性期患者辅助性T细胞亚群及其细胞因子IFN-γ、IL-4的变化及意义[J].北京医学,2016,38(2):105-109.
[7]于爱莲,王月丹,田喜凤,等.病原生物与免疫学[M].北京:北京大学医学出版社,2015:353.
[8]龚非力.医学免疫学[M].4版.北京:人民卫生出版社,2003:101-108.
[9]王朝辉,李洋,李威.急性脑血管病患者T淋巴细胞亚群变化的研究[J].中国老年学杂志,2006,26(9):1179-1180.
[10]杨丽娟,耿直.急性脑卒中患者T淋巴细胞亚群的变化[J].神经损伤与功能重建,2011,6(3):180-183.
[11]蒋福生,张平,张新华.急性脑梗死患者免疫调节性T淋巴细胞表达与预后的关系[J].中国动脉硬化杂志,2013,21(10):923-926.
[12]刘玉红,代瑞宁,傅佳.脑梗死患者颅内外动脉粥样硬化与T细胞亚群的相关性[J].中国动脉硬化杂志,2017,25(11):1152-1155.
[13]刘俊田.动脉粥样硬化发病的炎症机制的研究进展[J].西安交通大学学报(医学版),2015,36(2):141-152.
[14]聂大奥,陈俊抛,邢一兰.急性脑梗死患者外周血CD4CD25 T细胞和CD4CD28-T细胞的变化[J].中华老年心脑血管病杂志,2014,16(3):230-232.
[15]Tian Y,Liang X,Wu Y.The alternation of autophagy/apoptosis in CD4+CD25+Foxp3+Tregs on the developmental stages of atherosclerosis[J].Biomed Pharmacother,2017,97:1053-1060.
[16]Galkina E,Ley K.Immune and inflammatory mechanisms of atherosclerosis[J].Annu Rev Immunol,2009,27:165-197.
[17]李枚娟,巩燕,肖国胜,等.CD4~+T淋巴细胞Kv1.3通道在大鼠动脉粥样硬化中的作用[J].中国病理生理杂志,2010,26(9):1695-1699.