黑龙江省部分育成粳稻资源的遗传多样性分析
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摘要
为了阐明黑龙江省不同育种单位育成粳稻间的亲缘关系,本研究利用45对SSR多态性分子标记对收集于黑龙江省8个育种单位的104份粳稻资源的遗传多样性和群体结构进行了分析。结果表明,上述标记能够高效鉴别参试材料的多样性水平,共检测到154个等位基因,每个位点检测等位基因(Na)变异范围为2～8,平均为3.42;基因多样性指数(H)变异范围为0.02～0.77,平均为0.38;多态性信息含量(PIC)变异范围为0.02～0.73,平均为0.34。进一步分析发现,黑龙江省农业科学院黑河分院育成材料的遗传多样性水平较高,平均多态性信息含量(PIC)为0.33,而东北农业大学育成材料的多样性水平较低,平均多态性信息含量(PIC)为0.18。UPGMA聚类和遗传结构分析表明,黑龙江省农业科学院黑河分院材料与其他单位材料间亲缘关系较远,而黑龙江省农垦科学院水稻研究所与黑龙江省农业科学院佳木斯水稻研究所的材料间亲缘关系则较近。建议在今后的育种中,应该加强引入亲缘关系较远的外单位水稻种质资源用作杂交亲本,以期丰富本地区粳稻的遗传多样性水平。
The objective of this paper is to clarify the genetic relationship of japonica rice resources from different breeding units of Heilongjiang province.Genetic diversity and population structure of 104 japonica rice which collected from 8 different breeding units were analyzed by 45 pairs of polymorphisms molecular markers in this study.The results showed that all selected markers can be applied extensively for the genetic diversity level of rice,a total of 154 alleles were detected between these varieties,and the alleles(Na)per locus ranged from 2 to 8 with the mean value of 3.42.The gene diversity indices(H)varied from 0.02 to 0.77 with an average of 0.38.The polymorphism information content(PIC)was changed from 0.02 to 0.73 with the average of 0.34.Further analysis found that the genetic diversity of rice varieties from Heihe Branch of Heilongjiang Academy of Agricultural Sciences was highest,while the Northeast Agricultural University was lowest,the polymorphism information content(PIC)were 0.33 and 0.18,respectively.UPGMA Cluster and genetic structure analysis indicated that the rice cultivars had further relationships between Heihe Branch of Heilongjiang Academy of Agricultural Sciences and the other units,while there were closer relationship between Rice institute of Heilongjiang Agricultural Reclamation Academy and Jiamusi Rice Research Institute of Heilongjiang Academy of Agricultural Sciences.Thus,it is suggested that the breeders should strengthen the utilization efficiency of hybrid parents with large genetic differences from other units in future,so that it is possible to enrich the rice genetic diversity in this region.
The objective of this paper is to clarify the genetic relationship of japonica rice resources from different breeding units of Heilongjiang province.Genetic diversity and population structure of 104 japonica rice which collected from 8 different breeding units were analyzed by 45 pairs of polymorphisms molecular markers in this study.The results showed that all selected markers can be applied extensively for the genetic diversity level of rice,a total of 154 alleles were detected between these varieties,and the alleles(Na)per locus ranged from 2 to 8 with the mean value of 3.42.The gene diversity indices(H)varied from 0.02 to 0.77 with an average of 0.38.The polymorphism information content(PIC)was changed from 0.02 to 0.73 with the average of 0.34.Further analysis found that the genetic diversity of rice varieties from Heihe Branch of Heilongjiang Academy of Agricultural Sciences was highest,while the Northeast Agricultural University was lowest,the polymorphism information content(PIC)were 0.33 and 0.18,respectively.UPGMA Cluster and genetic structure analysis indicated that the rice cultivars had further relationships between Heihe Branch of Heilongjiang Academy of Agricultural Sciences and the other units,while there were closer relationship between Rice institute of Heilongjiang Agricultural Reclamation Academy and Jiamusi Rice Research Institute of Heilongjiang Academy of Agricultural Sciences.Thus,it is suggested that the breeders should strengthen the utilization efficiency of hybrid parents with large genetic differences from other units in future,so that it is possible to enrich the rice genetic diversity in this region.
引文
[1]束爱萍,金钟焕,张三元,曹桂兰,南钟浩,李圭星,卢勤,高熙宗,韩龙植.世界不同地理来源粳稻品种的遗传相似性研究.中国农业科学,2008,41(7):1879-1886Shu A P,Kim J H,Zhang S Y,Cao G L,Nan Z H,Lee K S,Lu Q,Koh H J,Han L Z.Genetic similarity analysis of japonica rice variety from different geography origin in the world.Scientia Agricultura Sinica,2008,41(7):1879-1886
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[6]国家水稻数据中心.中国水稻品种及其系谱数据库,http://www.ricedata.cn/variety/China Rice Data Center.Database of Chinese Rice Varieties and Genealogy,http://www.ricedata.cn/variety/
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[9]刘丹.东北粳稻遗传多样性及穗部性状基因定位研究.沈阳:沈阳农业大学,2014Liu D.Analysis of genetic diversity and gene mapping of panicle traits in japonica rice of northern China.Shenyang:Shenyang Agricultural University,2014
[10]李红宇,张龙海,刘梦红,徐正进,赵子成,王海泽,赵明辉,徐海,王嘉宇.利用SSR标记分析黑龙江水稻区域试验品系的遗传多样性.华北农学报,2012,27(2):105-110Li H Y,Zhang L H,Liu M H,Xu Z J,Zhao Z C,Wang HZ,Zhao M H,Xu H,Wang J Y.Microsatellite markerbased analysis of rice varieties in regional yield trial of Heilongjiang province.Acta Agriculturae Boreali-sinica,2012,27(2):105-110
[11]张立娜,曹桂兰,韩龙植.利用SSR标记揭示中国粳稻地方品种遗传多样性.中国农业科学,2012,45(3):405-413Zhang L N,Cao G L,Han L Z.Analysis of genetic diversity of japonica rice landrace in China with microsatellite marker.Scientia Agricultura Sinica,2012,45(3):405-413
[12]玄英实,姜文洙,刘宪虎,程正海,Hee-Jong Koh,元东林.中国东北地区水稻主栽品种的遗传多样性分析.植物遗传资源学报,2010,11(2):206-212Xuan Y S,Jiang W Z,Liu X H,Cheng Z H,Koh H J,Yuan D L.Comparative analysis of genetic diversity of commercial rice cultivars in northeastern China.Journal of Plant Genetic Resources,2010,11(2):206-212
[13]Gramene:A comparative resource for plants.[2018-07-01].http://news.gramene.org/
[14]Liu K,Muse S V.PowerMarker:an integrated analysis environment for genetic marker analysis.Bioinformatics,2005,21:2128-2129
[15]Tamura K,Dudley J,Nei M,Kumar S.MEGA4:molecular evolutionary genetics analysis(MEGA)software version4.0.Molecular Biology and Evolution,2007,24:1596-1599
[16]Evanno G,Regnaut S,Goudet J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study.Molecular Ecology,2005,14:2611-2620
[17]Earl D A,VonHoldt B M.STRUCTURE HARVESTER:a website and program for visualizing STRUCTURE output and implementing the Evanno method.Conservation Genetics Resources,2012,4(2):359-361
[18]刘化龙.寒地粳稻品种骨干亲本遗传演变及耐冷性研究.沈阳:沈阳农业大学,2012Liu H L.A study on heredity and cold-resistant capabilities of the founder parent strains of Japonica rice in cold regions of Heilongjiang.Shenyang:Shenyang Agricultural University,2012
[19]刘宝海,宋福金,高存启,于良斌.黑龙江大面积推广水稻品种遗传基础研究.作物杂志,2004(2):48-52Liu B H,Song F J,Gao C Q,Yu L B.Genetic basic research of widespread rice variety in Heilongjiang province.Crops,2004(2):48-52
[20]王黎明,李战胜,高旭华,沈雪峰,方越,陈勇.杂草稻、栽培稻及野生稻的遗传多样性比较.华中农业大学学报,2012,31(3):275-280Wang L M,Li Z S,Gao X H,Shen X F,Fang Y,Chen Y.Genetic diversities among weedy rices,cultivated rices and wild rices.Journal of Huazhong Agricultural University,2012,31(3):275-280
[21]刘丹,王嘉宇,马殿荣,孙健,柴永山,孙玉友,魏才强,解忠,李洪亮,张巍巍,程杜娟,孙国宏,陈温福.东北地区杂草稻与栽培稻的遗传多样性及籼粳分化.植物遗传资源学报,2017,18(2):217-224Liu D,Wang J Y,Ma D R,Sun J,Chai Y S,Sun Y Y,Wei CQ,Xie Z,Li H L,Zhang W W,Cheng D J,Sun G H,Chen WF.Genetic diversity and indica-japonica differentiation between weedy rice and cultivars in Northeast.Journal of Plant Genetic Resources,2017,18(2):217-224
[22]程芳艳,李春光,刘永巍,孙翊轩,王继亮,孟昭河,徐正进.寒地部分粳稻的遗传多样性及遗传结构分析.沈阳农业大学学报,2014,45(6):649-654Cheng F Y,Li C G,Liu Y W,Sun Y X,Wang J L,Meng Z H,Xu Z J.Analysis of genetic diversity and genetic structure about rice materials from cold region.Journal of Shenyang Agricultural University,2014,45(6):649-654
[2]孙健,王敬国,刘化龙,杨亮,赵雪,谢冬微,邹德堂.黑龙江省主栽水稻品种的遗传多样性分析.作物杂志,2011(1):63-66Sun J,Wang J G,Liu H L,Yang L,Zhao X,Xie D W,Zou D T.Analysis of genetic diversity of major rice cultivars in Heilongjiang province.Crops,2011(1):63-66
[3]杨静,刘海英,钱春荣,金正勋.黑龙江省水稻品种SSR标记遗传多样性分析.东北农业大学学报,2008,39(6):1-10Yang J,Liu H Y,Qian C R,Jin Z X.Analysis on genetic diversity of rice varieties in Heilongjiang province by using SSR.Journal of Northeast Agricultural University,2008,39(6):1-10
[4]张科,魏海锋,卓大龙,张晓敬,张帆,周永力,黎志康.黑龙江省近年审定水稻品种基于SSR标记的遗传多样性分析.植物遗传资源学报,2016,17(3):447-454Zhang K,Wei H F,Zhuo D L,Zhang X J,Zhang F,Zhou Y L,Li Z K.Genetic diversity analysis of registered rice(Oryza sativa L.)varieties in Heilongjiang province based on SSR markers.Journal of Plant Genetic Resources,2016,17(3):447-454
[5]徐振华,金正勋,张忠臣,刘海英,曲莹,王俊,郑艳双.黑龙江省粳稻种质资源遗传结构分析.作物杂志,2014(1):38-44Xu Z H,Jin Z X,Zhang Z C,Liu H Y,Qu Y,Wang J,Zheng Y S.Genetic structure assessment of japonica rice germplasm resources in Heilongjiang province.Crops,2014(1):38-44
[6]国家水稻数据中心.中国水稻品种及其系谱数据库,http://www.ricedata.cn/variety/China Rice Data Center.Database of Chinese Rice Varieties and Genealogy,http://www.ricedata.cn/variety/
[7]Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochem Bull,1987,19:11-15
[8]Jiang S K,Huang C,Zhang X J,Wang J Y,Chen W F,Xu ZJ.Development of a highly informative microsatellite(SSR)markers framework for rice(Oryza sativa L.)genotyping.Agricultural Science in China,2010,9(12):1697-1704
[9]刘丹.东北粳稻遗传多样性及穗部性状基因定位研究.沈阳:沈阳农业大学,2014Liu D.Analysis of genetic diversity and gene mapping of panicle traits in japonica rice of northern China.Shenyang:Shenyang Agricultural University,2014
[10]李红宇,张龙海,刘梦红,徐正进,赵子成,王海泽,赵明辉,徐海,王嘉宇.利用SSR标记分析黑龙江水稻区域试验品系的遗传多样性.华北农学报,2012,27(2):105-110Li H Y,Zhang L H,Liu M H,Xu Z J,Zhao Z C,Wang HZ,Zhao M H,Xu H,Wang J Y.Microsatellite markerbased analysis of rice varieties in regional yield trial of Heilongjiang province.Acta Agriculturae Boreali-sinica,2012,27(2):105-110
[11]张立娜,曹桂兰,韩龙植.利用SSR标记揭示中国粳稻地方品种遗传多样性.中国农业科学,2012,45(3):405-413Zhang L N,Cao G L,Han L Z.Analysis of genetic diversity of japonica rice landrace in China with microsatellite marker.Scientia Agricultura Sinica,2012,45(3):405-413
[12]玄英实,姜文洙,刘宪虎,程正海,Hee-Jong Koh,元东林.中国东北地区水稻主栽品种的遗传多样性分析.植物遗传资源学报,2010,11(2):206-212Xuan Y S,Jiang W Z,Liu X H,Cheng Z H,Koh H J,Yuan D L.Comparative analysis of genetic diversity of commercial rice cultivars in northeastern China.Journal of Plant Genetic Resources,2010,11(2):206-212
[13]Gramene:A comparative resource for plants.[2018-07-01].http://news.gramene.org/
[14]Liu K,Muse S V.PowerMarker:an integrated analysis environment for genetic marker analysis.Bioinformatics,2005,21:2128-2129
[15]Tamura K,Dudley J,Nei M,Kumar S.MEGA4:molecular evolutionary genetics analysis(MEGA)software version4.0.Molecular Biology and Evolution,2007,24:1596-1599
[16]Evanno G,Regnaut S,Goudet J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study.Molecular Ecology,2005,14:2611-2620
[17]Earl D A,VonHoldt B M.STRUCTURE HARVESTER:a website and program for visualizing STRUCTURE output and implementing the Evanno method.Conservation Genetics Resources,2012,4(2):359-361
[18]刘化龙.寒地粳稻品种骨干亲本遗传演变及耐冷性研究.沈阳:沈阳农业大学,2012Liu H L.A study on heredity and cold-resistant capabilities of the founder parent strains of Japonica rice in cold regions of Heilongjiang.Shenyang:Shenyang Agricultural University,2012
[19]刘宝海,宋福金,高存启,于良斌.黑龙江大面积推广水稻品种遗传基础研究.作物杂志,2004(2):48-52Liu B H,Song F J,Gao C Q,Yu L B.Genetic basic research of widespread rice variety in Heilongjiang province.Crops,2004(2):48-52
[20]王黎明,李战胜,高旭华,沈雪峰,方越,陈勇.杂草稻、栽培稻及野生稻的遗传多样性比较.华中农业大学学报,2012,31(3):275-280Wang L M,Li Z S,Gao X H,Shen X F,Fang Y,Chen Y.Genetic diversities among weedy rices,cultivated rices and wild rices.Journal of Huazhong Agricultural University,2012,31(3):275-280
[21]刘丹,王嘉宇,马殿荣,孙健,柴永山,孙玉友,魏才强,解忠,李洪亮,张巍巍,程杜娟,孙国宏,陈温福.东北地区杂草稻与栽培稻的遗传多样性及籼粳分化.植物遗传资源学报,2017,18(2):217-224Liu D,Wang J Y,Ma D R,Sun J,Chai Y S,Sun Y Y,Wei CQ,Xie Z,Li H L,Zhang W W,Cheng D J,Sun G H,Chen WF.Genetic diversity and indica-japonica differentiation between weedy rice and cultivars in Northeast.Journal of Plant Genetic Resources,2017,18(2):217-224
[22]程芳艳,李春光,刘永巍,孙翊轩,王继亮,孟昭河,徐正进.寒地部分粳稻的遗传多样性及遗传结构分析.沈阳农业大学学报,2014,45(6):649-654Cheng F Y,Li C G,Liu Y W,Sun Y X,Wang J L,Meng Z H,Xu Z J.Analysis of genetic diversity and genetic structure about rice materials from cold region.Journal of Shenyang Agricultural University,2014,45(6):649-654