玛咖对慢性束缚应激肝郁大鼠行为学及HPA轴的影响
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摘要
目的:观察玛咖对慢性束缚应激(CRS)肝郁大鼠行为及下丘脑-垂体-肾上腺轴(HPA)的影响,探讨新"外来中药"玛咖疏肝解郁功效及作用机制。方法:将雄性SD大鼠根据蔗糖水消耗量及体质量随机分组,每组10只。除空白组外,其余均采用孤养结合CRS复制肝郁模型。观察体质量,行为学指标,并用放射免疫法(RIA)检测大鼠下丘脑促肾上腺皮质激素释放素(CRH),血浆促肾上腺皮质激素(ACTH)、皮质酮(CORT)的含量。结果:造模第21天,与模型组比较,玛咖醇提高剂量组体质量、糖水消耗量明显增加(P<0.05),旷场实验穿行次数明显增加(P<0.01,P<0.05),高架十字迷宫实验开放臂进入次数和停留时间明显增加(P<0.05),玛咖醇提高剂量组下丘脑CRH,血浆ACTH、CORT的含量明显降低(P<0.05,P<0.01)。结论:玛咖对慢性束缚应激致大鼠肝郁模型具有调节作用,其疏肝解郁功效可能与抑制肝郁模型大鼠HPAA的激活有关。
Objective: To observe the effects of maca on behaviors and hypothalamic-pituitary-adrenal axis(HPAA) in chronic restraint stress(CRS) depressed rats and to explore the underlying mechanism. Methods: Male SD rats were randomly divided into eight groups according to the sucrose preference test and body weight(n=10). Except the normal control, the other groups were treated with the chronic stress stimulation combined with lonely. The behavior changes of rats were detected through open-field test, elevated plus-maze test, sucrose preference test and body weight were also measured. Radioimmunoassay(RIA) was used to analyze the levels of corticotrophin releasing hormone(CRH) in hypothalamus, adrenocorticotropic hormone(ACTH) and cortisol(CORT) in plasma. Results: Compared with model group, on the 21 th day, the weight and sugar consumption of high dose alcohol extract of maca group increased significantly(P<0.05). The cross times of open-field test of the maca group increased significantly(P<0.01, P<0.05). The times and stay period of entering open arms increased significantly(P<0.05), and the CRH, ACTH and CORT of the high dose alcohol extract of maca group decreased(P<0.05, P<0.01). Conclusion: Maca has a moderating effect on chronic restraint stress in stagnation of liver qi syndrome rats. Its function of soothing liver and relieving depression may be related to inhibiting the activation of HPAA in rats with liver stagnation.
Objective: To observe the effects of maca on behaviors and hypothalamic-pituitary-adrenal axis(HPAA) in chronic restraint stress(CRS) depressed rats and to explore the underlying mechanism. Methods: Male SD rats were randomly divided into eight groups according to the sucrose preference test and body weight(n=10). Except the normal control, the other groups were treated with the chronic stress stimulation combined with lonely. The behavior changes of rats were detected through open-field test, elevated plus-maze test, sucrose preference test and body weight were also measured. Radioimmunoassay(RIA) was used to analyze the levels of corticotrophin releasing hormone(CRH) in hypothalamus, adrenocorticotropic hormone(ACTH) and cortisol(CORT) in plasma. Results: Compared with model group, on the 21 th day, the weight and sugar consumption of high dose alcohol extract of maca group increased significantly(P<0.05). The cross times of open-field test of the maca group increased significantly(P<0.01, P<0.05). The times and stay period of entering open arms increased significantly(P<0.05), and the CRH, ACTH and CORT of the high dose alcohol extract of maca group decreased(P<0.05, P<0.01). Conclusion: Maca has a moderating effect on chronic restraint stress in stagnation of liver qi syndrome rats. Its function of soothing liver and relieving depression may be related to inhibiting the activation of HPAA in rats with liver stagnation.
引文
[1]王淳,张建军,王佳,等.玛咖的临床文献研究及其中药性能探讨.中华中医药杂志,2017,32(12):245-249
[2]张永忠,余龙江,万军梅,等.玛咖醇提物对正常小鼠免疫功能的影响.天然产物研究与开发,2007,19(2):274-276
[3]张洁宏,李彬,高玉秋,等.玛卡粉增强免疫力和抗疲劳功能动物实验研究.现代预防医学,2012,41(6):1096-1098
[4]胡远,李兴平,周毅,等.引种玛卡的抗疲劳实验研究.华西药学杂志,2015,30(4):440-441
[5]曹东,薛润光,顾鉴秋.丽江玛咖不同提取物对小鼠性行为及性激素的影响.云南中医中药杂志,2012,33(9):53-54
[6]Bo Lin Zheng,Kan He,Calvin Hyung,et al.Effect of a lipidic extract erom Lepidiummeyenii on sexual behavior in mice and rats.Urology,2000,55(4):598-602
[7]Gustavo F Gonzales,Ana Ruiz,Carla Gonzales,et al.Effect of Lepidiummeyenii(maca)roots on spermatogenesis of male rats.Asian J Androl,2001,9(3):231-233
[8]张永忠,程华.玛咖提取物的激素样活性实验研究.时珍国医国药,2008,19(8):1874-1875
[9]彭璐,赵云,许旭东,等.玛咖对维甲酸诱导的骨质疏松症的抑制作用.中药药理与临床,2014,30(3):98-101
[10]周意,栾洁,刘玉香,等.玛咖多糖对果蝇的抗衰老作用.中国实验方剂学杂志,2014,20(18):151-154
[11]张永忠,余龙江,金文闻,等.玛咖多糖抗氧化保健作用研究.食品科技,2005,30(8):97-99
[12]余龙江,张永忠,金文闻,等.玛咖醇提取物对小鼠的抗衰老作用.中草药,2006,37(1):81-83
[13]Rostislav Vecera,Jan Orolin,Nina Skottova,et al.The influence of maca(Lepidiummeyenii)on antioxidant status,lipid and glucose metabolism in rat.Plant Foods for Human Nutrition,2007,62(2):59-63
[14]张永忠,余龙江,敖明章,等.玛咖醇提取物对去卵巢大鼠内分泌激素及血脂水平的影响.中国新药杂志,2008,17(24):2112-2114,2121
[15]郑慧婷.玛咖提取物对氢溴酸东莨菪碱所致小鼠记忆获得障碍模型学习能力的影响.中医学报,2015,30(8):1170-1172
[16]Zhong Ai,Ai-Fang Cheng,Yuan-Tao Yu,et al.Antidepressantlike behavioral,anatomical,and biochemical effects of petroleum ether extract from maca(Lepidiummeyenii)in mice exposed to chronic unpredictable mild stress.Journal of Medicinal Food,2014,17(5):535-542
[17]王竹,杨晶明,王国栋,等.玛咖独行菜对卵巢切除术后大鼠血脂及骨质代谢的影响.卫生研究,2009,38(4):420-422,425
[18]Stoner G D,Kresty L A,Carlton P S,et al.Isothiocyanates and freezedried strawberries as inhibitors of esophageal cancer.Toxicol Sci,1999,52(2):95-100
[19]关于批准玛咖粉作为新资源食品的公告(2011年第13号).http://www.nhfpc.gov.cn/sps/s7891/201106/d18bef8a697e44da87f20ebb41cb428e.shtm L,2011-6-16
[20]张建军,王淳,费文婷,等.外来药物”中药化”的研究思路与策略.中华中医药杂志,2017,32(4):1425-1430
[21]Yan T M,Fan W T,Liu L,et al.The effects of Xingnao Jieyu capsules on post-stroke depression are similar to those of fluoxetine.Neural Regen Res,2013,8(19):1765-1772
[22]胡海燕,徐志伟,敖海清,等.四逆散影响慢性应激抑郁模型大鼠行为学研究.中华中医药杂志,2014,29(1):72-74
[23]Kaye J,Morton J,Bowcutt M,et al.Stress,depression and psychoneuroimmunology.J Neurosci Nurs,2000,32(2):39-52
[24]王天芳,陈易新,季绍良,等.慢性束缚致慢性疲劳动物模型的研制及其行为学观察.中国中医基础医学杂志,1995,5(5):25-29
[25]梁媛,SOLOS Ioannis,陈家旭,等.逍遥散对抑郁脾虚证模型大鼠行为学变化的调节作用.北京中医药大学学报,2010,33(5):317-322
[26]Julio Rubio,Maria Caldas,Sonia Dávila.Effect of three different cultivars of Lepidium meyenii(Maca)on learning and depression in ovariectomized mice.BMC Complementary and Alternative Medicine,2006,6:23
[27]L Stojanovska,C Law,B Lai.Maca reduces blood pressure and depression,in a pilot study in postmenopausal women.CLIMACTERIC,2014,17:1-10
[28]李玉波,马雪玲,武志黔,等.抑郁症肝郁脾虚证大鼠中枢及外周5-羟色胺的变化规律研究.中华中医药杂志,2015,28(5):1267-1271
[29]Binder E B,Nemeroff C B.The CRF system,stress,depression and anxiety-insights from human genetic studies.Mol Psychiatry,2015,15(6):574-588
[30]王景霞,张建军,李伟,等.白芍提取物对慢性应激抑郁模型大鼠行为学及大脑皮层单胺类神经递质的影响.中华中医药杂志,2010,25(11):1895-1897
[31]王景霞,张建军,苗春平,等.白芍提取物对嗅球损毁抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响.中国实验方剂学杂志,2011,17(3):155-158
[32]王景霞,张建军,李伟,等.白芍提取物治疗抑郁症的实验研究的影响.中国实验方剂学杂志,2010,16(7):183-184
[33]王景霞,张建军,李伟,等.芍药苷抗抑郁作用与NO/c GMP通路相关性研究.中药与临床,2012,3(1):27-28,37
[34]崔广智.芍药苷抗抑郁作用的实验研究.现代药物与临床,2009,24(4):231-233
[2]张永忠,余龙江,万军梅,等.玛咖醇提物对正常小鼠免疫功能的影响.天然产物研究与开发,2007,19(2):274-276
[3]张洁宏,李彬,高玉秋,等.玛卡粉增强免疫力和抗疲劳功能动物实验研究.现代预防医学,2012,41(6):1096-1098
[4]胡远,李兴平,周毅,等.引种玛卡的抗疲劳实验研究.华西药学杂志,2015,30(4):440-441
[5]曹东,薛润光,顾鉴秋.丽江玛咖不同提取物对小鼠性行为及性激素的影响.云南中医中药杂志,2012,33(9):53-54
[6]Bo Lin Zheng,Kan He,Calvin Hyung,et al.Effect of a lipidic extract erom Lepidiummeyenii on sexual behavior in mice and rats.Urology,2000,55(4):598-602
[7]Gustavo F Gonzales,Ana Ruiz,Carla Gonzales,et al.Effect of Lepidiummeyenii(maca)roots on spermatogenesis of male rats.Asian J Androl,2001,9(3):231-233
[8]张永忠,程华.玛咖提取物的激素样活性实验研究.时珍国医国药,2008,19(8):1874-1875
[9]彭璐,赵云,许旭东,等.玛咖对维甲酸诱导的骨质疏松症的抑制作用.中药药理与临床,2014,30(3):98-101
[10]周意,栾洁,刘玉香,等.玛咖多糖对果蝇的抗衰老作用.中国实验方剂学杂志,2014,20(18):151-154
[11]张永忠,余龙江,金文闻,等.玛咖多糖抗氧化保健作用研究.食品科技,2005,30(8):97-99
[12]余龙江,张永忠,金文闻,等.玛咖醇提取物对小鼠的抗衰老作用.中草药,2006,37(1):81-83
[13]Rostislav Vecera,Jan Orolin,Nina Skottova,et al.The influence of maca(Lepidiummeyenii)on antioxidant status,lipid and glucose metabolism in rat.Plant Foods for Human Nutrition,2007,62(2):59-63
[14]张永忠,余龙江,敖明章,等.玛咖醇提取物对去卵巢大鼠内分泌激素及血脂水平的影响.中国新药杂志,2008,17(24):2112-2114,2121
[15]郑慧婷.玛咖提取物对氢溴酸东莨菪碱所致小鼠记忆获得障碍模型学习能力的影响.中医学报,2015,30(8):1170-1172
[16]Zhong Ai,Ai-Fang Cheng,Yuan-Tao Yu,et al.Antidepressantlike behavioral,anatomical,and biochemical effects of petroleum ether extract from maca(Lepidiummeyenii)in mice exposed to chronic unpredictable mild stress.Journal of Medicinal Food,2014,17(5):535-542
[17]王竹,杨晶明,王国栋,等.玛咖独行菜对卵巢切除术后大鼠血脂及骨质代谢的影响.卫生研究,2009,38(4):420-422,425
[18]Stoner G D,Kresty L A,Carlton P S,et al.Isothiocyanates and freezedried strawberries as inhibitors of esophageal cancer.Toxicol Sci,1999,52(2):95-100
[19]关于批准玛咖粉作为新资源食品的公告(2011年第13号).http://www.nhfpc.gov.cn/sps/s7891/201106/d18bef8a697e44da87f20ebb41cb428e.shtm L,2011-6-16
[20]张建军,王淳,费文婷,等.外来药物”中药化”的研究思路与策略.中华中医药杂志,2017,32(4):1425-1430
[21]Yan T M,Fan W T,Liu L,et al.The effects of Xingnao Jieyu capsules on post-stroke depression are similar to those of fluoxetine.Neural Regen Res,2013,8(19):1765-1772
[22]胡海燕,徐志伟,敖海清,等.四逆散影响慢性应激抑郁模型大鼠行为学研究.中华中医药杂志,2014,29(1):72-74
[23]Kaye J,Morton J,Bowcutt M,et al.Stress,depression and psychoneuroimmunology.J Neurosci Nurs,2000,32(2):39-52
[24]王天芳,陈易新,季绍良,等.慢性束缚致慢性疲劳动物模型的研制及其行为学观察.中国中医基础医学杂志,1995,5(5):25-29
[25]梁媛,SOLOS Ioannis,陈家旭,等.逍遥散对抑郁脾虚证模型大鼠行为学变化的调节作用.北京中医药大学学报,2010,33(5):317-322
[26]Julio Rubio,Maria Caldas,Sonia Dávila.Effect of three different cultivars of Lepidium meyenii(Maca)on learning and depression in ovariectomized mice.BMC Complementary and Alternative Medicine,2006,6:23
[27]L Stojanovska,C Law,B Lai.Maca reduces blood pressure and depression,in a pilot study in postmenopausal women.CLIMACTERIC,2014,17:1-10
[28]李玉波,马雪玲,武志黔,等.抑郁症肝郁脾虚证大鼠中枢及外周5-羟色胺的变化规律研究.中华中医药杂志,2015,28(5):1267-1271
[29]Binder E B,Nemeroff C B.The CRF system,stress,depression and anxiety-insights from human genetic studies.Mol Psychiatry,2015,15(6):574-588
[30]王景霞,张建军,李伟,等.白芍提取物对慢性应激抑郁模型大鼠行为学及大脑皮层单胺类神经递质的影响.中华中医药杂志,2010,25(11):1895-1897
[31]王景霞,张建军,苗春平,等.白芍提取物对嗅球损毁抑郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响.中国实验方剂学杂志,2011,17(3):155-158
[32]王景霞,张建军,李伟,等.白芍提取物治疗抑郁症的实验研究的影响.中国实验方剂学杂志,2010,16(7):183-184
[33]王景霞,张建军,李伟,等.芍药苷抗抑郁作用与NO/c GMP通路相关性研究.中药与临床,2012,3(1):27-28,37
[34]崔广智.芍药苷抗抑郁作用的实验研究.现代药物与临床,2009,24(4):231-233
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