土壤酶活性对沙地樟子松人工林衰退的响应
|
摘要
采用时空互代法对辽宁省章古台地区10—60 a林龄的典型沙地樟子松人工林标准地土壤酶活性进行了测定,分析了土壤酶活性在时间(不同林龄)、空间(不同土层深度)的变化规律,及养分指标与土壤酶活性的关系,为分析樟子松人工林土壤退化问题及本研究区域土壤酶的后续研究提供参考.结果表明,该区域樟子松人工林土壤蔗糖酶和蛋白酶活性随林分年龄的增长而增高;土壤磷酸酶和过氧化氢酶活性先增高后降低趋势,在30 a林龄时分别达到最高值;土壤脲酶活性随林分年龄的增长呈先降低,30 a后保持稳定的趋势.5种土壤酶活性均随土层的加深而降低,且表层土壤酶活性显著高于20 cm以下土层土壤酶活性.随林龄和土层的递增,土壤酶指数与理化指标的变化趋势相似,说明土壤理化性状对土壤酶活性高低起重要作用.相关性分析表明,各土壤酶活性与养分因子间呈极显著相关,可用其表征林地土壤肥力状况.综上所述,可以从改善林地土壤理化性状入手,减缓樟子松人工林的提前衰退,而评价该区域林地土壤综合质量可采用测取土壤酶活性的方法.
Soil enzyme activities of typical Pinus sylvestris var. mongolica plantations aged 10—60 years in Zhanggutai of Liaoning Province were determined using time-space substitution method.The changes of soil enzyme activities with time( different ages) and location( different depths of soil layers),and the relationships between nutrients and soil enzyme activity were analyzed. The purpose was to study soil degradation of Pinus sylvestris var. mongolica plantations and provide reference for the future study of soil enzyme in the study area. The results showed that the activities of sucrase and protease in the Pinus sylvestris var. mongolica plantations increased with the increase of stand age.The activities of phosphatase and catalase increased at first and then decreased,and reached the highest respectively at the age of 30 years. The soil urease activity increased,and then decreasedwith the increase of stand age,and remained unchanged at the age of 30 years. The activities of five soil enzymes decreased with the deepening of soil layers,and the surface soil enzyme activity was significantly higher than soil enzyme activity below 20 cm soil layer. With the increase of stand age and soil layer,the change of soil enzyme and physicochemical was similar,indicating that soil physical and chemical properties played an important role in soil enzyme activity. The correlation analysis showed that there was a significant correlation between soil enzyme activities and nutrients,which can be used to characterize soil fertility in forest lands. In summary,the physicochemical properties of forest soil can be improved to reduce the early decline of Pinus sylvestris var. mongolica plantations,and the method of measuring soil enzyme activity can be used to evaluate the comprehensive quality of forest soil in the region.
Soil enzyme activities of typical Pinus sylvestris var. mongolica plantations aged 10—60 years in Zhanggutai of Liaoning Province were determined using time-space substitution method.The changes of soil enzyme activities with time( different ages) and location( different depths of soil layers),and the relationships between nutrients and soil enzyme activity were analyzed. The purpose was to study soil degradation of Pinus sylvestris var. mongolica plantations and provide reference for the future study of soil enzyme in the study area. The results showed that the activities of sucrase and protease in the Pinus sylvestris var. mongolica plantations increased with the increase of stand age.The activities of phosphatase and catalase increased at first and then decreased,and reached the highest respectively at the age of 30 years. The soil urease activity increased,and then decreasedwith the increase of stand age,and remained unchanged at the age of 30 years. The activities of five soil enzymes decreased with the deepening of soil layers,and the surface soil enzyme activity was significantly higher than soil enzyme activity below 20 cm soil layer. With the increase of stand age and soil layer,the change of soil enzyme and physicochemical was similar,indicating that soil physical and chemical properties played an important role in soil enzyme activity. The correlation analysis showed that there was a significant correlation between soil enzyme activities and nutrients,which can be used to characterize soil fertility in forest lands. In summary,the physicochemical properties of forest soil can be improved to reduce the early decline of Pinus sylvestris var. mongolica plantations,and the method of measuring soil enzyme activity can be used to evaluate the comprehensive quality of forest soil in the region.
引文
[1]康宏樟,朱教君,许美玲.科尔沁沙地樟子松人工林幼树水分生理生态特性[J].干旱区研究,2007,24(1):15-22.KANG H Z,ZHU J J,XU M L. Study on water physiological properties of the artificially-planned saplings of Pinus sylvestris var. mongolica in the Horqin sandland[J]. Arid Zone Research,2007,24(1):15-22(in Chinese).
[2]孟鹏,李玉灵,尤国春,等.彰武松、樟子松光合生产与蒸腾耗水特性[J].生态学报,2012,32(10):3050-3060.MENG P,LI Y L,YOU G C,et al. Characteristics of photosynthetic productivity and water-consumption for transpiration in Pinus densiflora var. zhangwuensis and Pinus sylvestris var.mongolica[J]. Acta Ecologica Sinica,2012,32(10):3050-3060(in Chinese).
[3]焦树仁.辽宁省章古台樟子松固沙林提早衰弱的原因与防治措施[J].林业科学,2001,37(2):131-138.JIAO S R. Report on the causes of the early decline of Pinus sylvestris var. mongolica shelterbelt and its preventative and control measures in Zhanggutai of Liaoning Province[J]. Scientia Silvae Sinicae,2001,37(2):131-138(in Chinese).
[4]牛沙沙,周永斌,刘丽颖,等.不同林龄樟子松人工林土壤理化性质[J].东北林业大学学报,2015,43(2):47-50.NIU S S,ZHOU Y B,LIU L Y,et al. Soil properties in Pinus sylvestris var. mongolica plantation of different ages[J]. Journal of Northeast Forestry University,2015,43(2):47-50(in Chinese).
[5]薛建辉,王智,吕祥生.林木根系与土壤环境相互作用研究综述[J].南京林业大学学报,2002,26(3):79-84.XUE J H,WANG Z,LU X S. Progress on the interaction between tree roots and soil environment[J]. Journal of Nanjing Forestry Unversity,2002,26(3):79-84(in Chinese).
[6]孔涛,刘民,淑敏,等.低分子量有机酸对土壤微生物数量和酶活性的影响[J].环境化学,2016,35(2):348-354.KONG T,LIU M,SHU M,et al. Effect of low molecular weight organic acids on soil microbe number and soil enzyme activities[J].Environmental Chemistry,2016,35(2):348-354(in Chinese).
[7]高惠民.农业土壤管理[M].北京:中国农业科技出版社,1988:37-42.GAO H M. Agricultural Soil Management[M]. Beijing:China Agricultural Science and Technology Press,1988:37-42(in Chinese).
[8]薛冬,姚槐应,黄昌勇.植茶年龄对茶园土壤微生物特性及酶活性的影响[J].水土保持学报,2005,19(2):84-87.XUE D,YAO H Y,HUANG C Y. Study on soil microbial properties and enzyme activities in tea gardens[J]. Journal of Soil Water Conservation,2005,19(2):84-87(in Chinese).
[9]王少昆,赵学勇,张铜会,等.造林对沙地土壤微生物的数量、生物量碳及酶活性的影响[J].中国沙漠,2013,33(2):529-535.WANG S K,ZHAO X Y,ZHANG T H,et al. Effects of afforestation on the abundance,biomass carbon and enzymatic activities of soil microorganism in sandy dunes[J]. Journal of Desert Research,2013,33(2):529-535(in Chinese).
[10]王伟东,王渭玲,徐福利,等.秦岭西部中幼龄华北落叶松林地土壤养分与酶活性特征研究[J].植物营养与肥料学报,2015,21(4):1032-1039.WANG W D,WANG W L,XU F L,et al. Characteristics of soil nutrients and enzyme activities in young and middle aged plantation in western Qinling Mountains[J]. Journal of Plant Nutrition and Fertilizer Science,2015,21(4):1032-1039(in Chinese).
[11]杨晓娟,廖超英,李晓明,等.榆林沙区樟子松林土壤酶活性与土壤化学性质的通径分析[J].干旱区资源与环境,2013,27(9):109-112.YANG X J,LIAO C Y,LI X M,et al. Path analysis of soil enzyme activities and soil chemical properties of Pinus sytvestris var. mongolicain sandy area of Yulin[J]. Journal of Arid Land Resources&Environment,2013,27(9):109-112(in Chinese).
[12]徐恒,廖超英,李晓明,等.榆林沙区人工固沙林土壤微生物生态分布特征及酶活性研究[J].西北农林科技大学学报,2008,36(12):135-141.XU H,LIAO C Y,LI X M,et al. Soil microorganism and enzyme activities under different artificial sand-fixing vegetations in the sandy area of Yulin[J]. Journal of Northwest A&F University,2008,36(12):135-141(in Chinese).
[13]魏晓婷,雷泽勇,韩辉.章古台沙地不同林龄樟子松人工林土壤水分研究[J].干旱区资源与环境,2016,30(6):115-121.WEI X T,LEI Z Y,HAN H. Soil moistures characteristics of different-aged Pinus sylvestris var. mongolica artificial forests in Zhanggutai sandy area[J]. Journal of Arid Land Resources&Environment,2016,30(6):115-121(in Chinese).
[14]赵兰坡,姜岩.土壤磷酸酶活性测定方法的探讨[J].土壤通报,1986,17(3):138-141.ZHAO L P,JIANG Y. Determination of soil phosphatase activity[J]. Chinese Journal of Soil Science.,1986,17(3):138-141(in Chinese).
[15] SHAHRIARI F,HIGASHI T,TAMURA K. Effects of clay addition on soil protease activities in Andosols in the presence of cadmium[J].Soil Science&Plant Nutrition,2010,56(4):560-569.
[16] NARASIMHAA G,SRIDEVIB A,REDDY S V G. et al. Effect of cotton ginning mill industrial effluents on soil dehydrogenase,phosphatase,amylase and invertase enzyme activities[J]. International Journal of Agricultural and Food Science,2012,2(1):1-6.
[17] Kl Zl LKAYA R,AKA 6)I,A爦Kl N T,et al. Effect of soil contamination with azadirachtin on dehydrogenase and catalase activity of soil[J].Eurasian Journal of Soilence,2012,1(2):98-103.
[18]孔涛,刘民,马瑜,等.黄瓜产量及土壤酶活性对基于古龙酸母液与废弃培养基有机肥的响应[J].环境化学,2015,34(12):2275-2280.KONG T,LIU M,MA Y,et al. Response of cucumber yield and soil enzyme activities to organic fertilizer of residue after evaporation from vitamin C fermentation and abandoned medium[J]. Environmental Chemistry,2015,34(12):2275-2280(in Chinese).
[19]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2005:25-114.Bao S D. Soil agro-chemistrical analysis[M]. Beijing:China Agriculture Press,2005:25-114(in Chinese).
[20]张超,刘国彬,薛萧,等.黄土氏陵区不同林龄人工刺槐林土壤酶演变特征[J].林业科学,2010,46(12):23-29.ZHANG C,LIU G B,XUE X,et al. Evolution of soil enzyme activities of Robinia pseudoacacia plantation at different ages in Loess hilly region[J]. Scientia Silvae Sinicae,2010,46(12):23-29(in Chinese).
[21]叶存旺,翟巧绒,郭梓娟,等.沙棘一侧柏混交林土壤养分、微生物与酶活性的研究[J].西北林学院学报,2007,22(5):1-6.YE C W,ZHAI Q R,GUO Z J,et al. Soil nutrient,microorganism and enzyme activity of Hippophae rhamnoides and Platycladus orientalis mixed forests[J]. Journal of Northwest Forestry University,2007,22(5):1-6(in Chinese).
[22]赵燕娜,廖超英,李晓明,等.毛乌素沙地不同林龄樟子松人工林土壤酶活性变化特征[J].西北林学院学报,2014,29(2):1-5.ZHAO Y N,LIAO C Y,LI X M,et al. Evolution of soil enzyme activities of Pinus sytvestris var. mongolica plantation at different ages in Mu us sandy land[J]. Journal of Northwest Forestry University,2014,29(2):1-5(in Chinese).
[23]宋立宁,朱教君,郑晓.基于沙地樟子松人工林衰退机制的营林方案[J].生态学杂志,2017,36(11):3249-3256.SONG L N,ZHU J J,ZHENG X. Forestation and management scheme of Pinus sylvestris var. mongolica plantations in sandy lands based on their decline mechanisms[J]. Chinese Journal of Ecology,2017,36(11):3249-3256(in Chinese).
[24]张威,张明,张旭东,等.土壤蛋白酶和芳香氨基酶的研究进展[J].土壤通报,2008,39(6):1468-1474.ZHANG W,ZHANG M,ZHANG X D,et al. A review on soil protease and arylamidase[J]. Chinese Journal of Soil Science,2008,39(6):1468-1474(in Chinese).
[25] KAMIMURA Y,HAYANO K. Properties of protease extracted from tea-field soil[J]. Biology&Fertility of Soils,2000,30(4):351-355.
[26]钱洲,俞元春,俞小鹏,等.毛乌素沙地飞播造林植被恢复过程土壤酶活性的变化[J].水土保持研究,2014,21(6):95-100.QIAN Z,YU Y C,YU X P,et al. Changes of vegetation characteristics and soil properties in Mu Us sandy land by aerial seeding afforestation[J]. Journal of Central South University of Forestry&Technology,2014,21(6):95-100(in Chinese).
[27]余娜,刘济明,张超,等.不同沙生植被土壤酶活性分异特征研究[J].水土保持研究,2010,17(1):77-79.YU N,LIU J M,ZHANG C,et al. Study on the differentiation characteristics of different sandy vegetation soil enzyme activities[J].Research of Soil&Water Conservation,2010,17(1):77-79(in Chinese).
[28]姜凤岐,曾德慧,于占源.从恢复生态学视角透析防护林衰退及其防治对策:以章古台地区樟子松林为例[J].应用生态学报,2006,17(12):2229-2235.JIANG F Q,ZENG D H,YU Z Y. Decline of protective forest and its prevention strategies from viewpoint of restoration ecology:Taking Pinus sylvestris var. mongolica plantation in Zhanggutai as an example[J]. Chinese Journal of Applied Ecology,2006,17(12):2229-2235(in Chinese).
[29]王兵,刘国彬,薛芝,等.黄土丘陵区撂荒对土壤酶活性的影响[J].草地学报,2009,17(3):282-287.WANG B,LIU G B,XUE Z,et al. Effect of farmland abandonment on soil enzyme activities in Loess hilly region[J]. Acta Agrestia Sinica,2009,17(3):282-287(in Chinese).
[30]杨万勤,王开运.土壤酶研究动态与展望[J].应用与环境生物学报,2002,8(5):564-570.YANG W Q,WANG K Y. Advances on soil enzymology[J]. Chinese Journal of Applied&Environmental Biology,2002,8(5):564-570(in Chinese).
[31]杨万勤,宋光煜,韩玉萍.土壤生态学的理论体系及其研究领域[J].生态学杂志,2000,19(4):53-56.YANG W Q,SONG G Y,HAN Y P. Theoretical system and research fields of soil ecology[J]. Chinese Journal of Ecology,2000,19(4):53-56(in Chinese).
[32]杨涛,徐慧,方德华,等.樟子松林下土壤养分、微生物及酶活性的研究[J].土壤通报,2006,37(2):2253-2257.YANG T,XU H,FANG D H,et al. Soil nutrient,microorganism and enzyme activity in Pinus sylvestris var. mongolica forests[J]. Chinese Journal of Soil Science,2006,37(2):2253-2257(in Chinese).
[33]耿玉清,白翠霞,赵广亮,等.土壤磷酸酶活性及其与有机磷组分的相关性[J].北京林业大学学报,2008,30(S2):139-143.GENG Y Q,BAI C X,ZHAO G L,et al. Soil phosphatase activity and its correlation with composition of organic phosphorus[J]. Journal of Beijing Forestry University,2008,30(S2):139-143(in Chinese).
[34]王素娟,高丽,苏和,等.内蒙古库布齐沙地土壤蛋白酶初步研究[J].草业科学,2009,26(9):13-17.WANG S J,GAO L,SU H,et al. The preliminary research of soil prolease on Khobqi sand area of Inner Mongolia[J]. Pratacultural Science,2009,26(9):13-17(in Chinese).
[35]王灿,王德建,孙瑞娟.长期不同施肥方式下土壤酶活性与肥力因素的相关性[J].生态环境,2008,17(2):688-692.WANG C,WANG D J,SUN R J. The relationship between soil enzyme activities and soil nutrients by long-term fertilizer experiments[J].Ecology&Environment,2008,17(2):688-692(in Chinese).
[2]孟鹏,李玉灵,尤国春,等.彰武松、樟子松光合生产与蒸腾耗水特性[J].生态学报,2012,32(10):3050-3060.MENG P,LI Y L,YOU G C,et al. Characteristics of photosynthetic productivity and water-consumption for transpiration in Pinus densiflora var. zhangwuensis and Pinus sylvestris var.mongolica[J]. Acta Ecologica Sinica,2012,32(10):3050-3060(in Chinese).
[3]焦树仁.辽宁省章古台樟子松固沙林提早衰弱的原因与防治措施[J].林业科学,2001,37(2):131-138.JIAO S R. Report on the causes of the early decline of Pinus sylvestris var. mongolica shelterbelt and its preventative and control measures in Zhanggutai of Liaoning Province[J]. Scientia Silvae Sinicae,2001,37(2):131-138(in Chinese).
[4]牛沙沙,周永斌,刘丽颖,等.不同林龄樟子松人工林土壤理化性质[J].东北林业大学学报,2015,43(2):47-50.NIU S S,ZHOU Y B,LIU L Y,et al. Soil properties in Pinus sylvestris var. mongolica plantation of different ages[J]. Journal of Northeast Forestry University,2015,43(2):47-50(in Chinese).
[5]薛建辉,王智,吕祥生.林木根系与土壤环境相互作用研究综述[J].南京林业大学学报,2002,26(3):79-84.XUE J H,WANG Z,LU X S. Progress on the interaction between tree roots and soil environment[J]. Journal of Nanjing Forestry Unversity,2002,26(3):79-84(in Chinese).
[6]孔涛,刘民,淑敏,等.低分子量有机酸对土壤微生物数量和酶活性的影响[J].环境化学,2016,35(2):348-354.KONG T,LIU M,SHU M,et al. Effect of low molecular weight organic acids on soil microbe number and soil enzyme activities[J].Environmental Chemistry,2016,35(2):348-354(in Chinese).
[7]高惠民.农业土壤管理[M].北京:中国农业科技出版社,1988:37-42.GAO H M. Agricultural Soil Management[M]. Beijing:China Agricultural Science and Technology Press,1988:37-42(in Chinese).
[8]薛冬,姚槐应,黄昌勇.植茶年龄对茶园土壤微生物特性及酶活性的影响[J].水土保持学报,2005,19(2):84-87.XUE D,YAO H Y,HUANG C Y. Study on soil microbial properties and enzyme activities in tea gardens[J]. Journal of Soil Water Conservation,2005,19(2):84-87(in Chinese).
[9]王少昆,赵学勇,张铜会,等.造林对沙地土壤微生物的数量、生物量碳及酶活性的影响[J].中国沙漠,2013,33(2):529-535.WANG S K,ZHAO X Y,ZHANG T H,et al. Effects of afforestation on the abundance,biomass carbon and enzymatic activities of soil microorganism in sandy dunes[J]. Journal of Desert Research,2013,33(2):529-535(in Chinese).
[10]王伟东,王渭玲,徐福利,等.秦岭西部中幼龄华北落叶松林地土壤养分与酶活性特征研究[J].植物营养与肥料学报,2015,21(4):1032-1039.WANG W D,WANG W L,XU F L,et al. Characteristics of soil nutrients and enzyme activities in young and middle aged plantation in western Qinling Mountains[J]. Journal of Plant Nutrition and Fertilizer Science,2015,21(4):1032-1039(in Chinese).
[11]杨晓娟,廖超英,李晓明,等.榆林沙区樟子松林土壤酶活性与土壤化学性质的通径分析[J].干旱区资源与环境,2013,27(9):109-112.YANG X J,LIAO C Y,LI X M,et al. Path analysis of soil enzyme activities and soil chemical properties of Pinus sytvestris var. mongolicain sandy area of Yulin[J]. Journal of Arid Land Resources&Environment,2013,27(9):109-112(in Chinese).
[12]徐恒,廖超英,李晓明,等.榆林沙区人工固沙林土壤微生物生态分布特征及酶活性研究[J].西北农林科技大学学报,2008,36(12):135-141.XU H,LIAO C Y,LI X M,et al. Soil microorganism and enzyme activities under different artificial sand-fixing vegetations in the sandy area of Yulin[J]. Journal of Northwest A&F University,2008,36(12):135-141(in Chinese).
[13]魏晓婷,雷泽勇,韩辉.章古台沙地不同林龄樟子松人工林土壤水分研究[J].干旱区资源与环境,2016,30(6):115-121.WEI X T,LEI Z Y,HAN H. Soil moistures characteristics of different-aged Pinus sylvestris var. mongolica artificial forests in Zhanggutai sandy area[J]. Journal of Arid Land Resources&Environment,2016,30(6):115-121(in Chinese).
[14]赵兰坡,姜岩.土壤磷酸酶活性测定方法的探讨[J].土壤通报,1986,17(3):138-141.ZHAO L P,JIANG Y. Determination of soil phosphatase activity[J]. Chinese Journal of Soil Science.,1986,17(3):138-141(in Chinese).
[15] SHAHRIARI F,HIGASHI T,TAMURA K. Effects of clay addition on soil protease activities in Andosols in the presence of cadmium[J].Soil Science&Plant Nutrition,2010,56(4):560-569.
[16] NARASIMHAA G,SRIDEVIB A,REDDY S V G. et al. Effect of cotton ginning mill industrial effluents on soil dehydrogenase,phosphatase,amylase and invertase enzyme activities[J]. International Journal of Agricultural and Food Science,2012,2(1):1-6.
[17] Kl Zl LKAYA R,AKA 6)I,A爦Kl N T,et al. Effect of soil contamination with azadirachtin on dehydrogenase and catalase activity of soil[J].Eurasian Journal of Soilence,2012,1(2):98-103.
[18]孔涛,刘民,马瑜,等.黄瓜产量及土壤酶活性对基于古龙酸母液与废弃培养基有机肥的响应[J].环境化学,2015,34(12):2275-2280.KONG T,LIU M,MA Y,et al. Response of cucumber yield and soil enzyme activities to organic fertilizer of residue after evaporation from vitamin C fermentation and abandoned medium[J]. Environmental Chemistry,2015,34(12):2275-2280(in Chinese).
[19]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2005:25-114.Bao S D. Soil agro-chemistrical analysis[M]. Beijing:China Agriculture Press,2005:25-114(in Chinese).
[20]张超,刘国彬,薛萧,等.黄土氏陵区不同林龄人工刺槐林土壤酶演变特征[J].林业科学,2010,46(12):23-29.ZHANG C,LIU G B,XUE X,et al. Evolution of soil enzyme activities of Robinia pseudoacacia plantation at different ages in Loess hilly region[J]. Scientia Silvae Sinicae,2010,46(12):23-29(in Chinese).
[21]叶存旺,翟巧绒,郭梓娟,等.沙棘一侧柏混交林土壤养分、微生物与酶活性的研究[J].西北林学院学报,2007,22(5):1-6.YE C W,ZHAI Q R,GUO Z J,et al. Soil nutrient,microorganism and enzyme activity of Hippophae rhamnoides and Platycladus orientalis mixed forests[J]. Journal of Northwest Forestry University,2007,22(5):1-6(in Chinese).
[22]赵燕娜,廖超英,李晓明,等.毛乌素沙地不同林龄樟子松人工林土壤酶活性变化特征[J].西北林学院学报,2014,29(2):1-5.ZHAO Y N,LIAO C Y,LI X M,et al. Evolution of soil enzyme activities of Pinus sytvestris var. mongolica plantation at different ages in Mu us sandy land[J]. Journal of Northwest Forestry University,2014,29(2):1-5(in Chinese).
[23]宋立宁,朱教君,郑晓.基于沙地樟子松人工林衰退机制的营林方案[J].生态学杂志,2017,36(11):3249-3256.SONG L N,ZHU J J,ZHENG X. Forestation and management scheme of Pinus sylvestris var. mongolica plantations in sandy lands based on their decline mechanisms[J]. Chinese Journal of Ecology,2017,36(11):3249-3256(in Chinese).
[24]张威,张明,张旭东,等.土壤蛋白酶和芳香氨基酶的研究进展[J].土壤通报,2008,39(6):1468-1474.ZHANG W,ZHANG M,ZHANG X D,et al. A review on soil protease and arylamidase[J]. Chinese Journal of Soil Science,2008,39(6):1468-1474(in Chinese).
[25] KAMIMURA Y,HAYANO K. Properties of protease extracted from tea-field soil[J]. Biology&Fertility of Soils,2000,30(4):351-355.
[26]钱洲,俞元春,俞小鹏,等.毛乌素沙地飞播造林植被恢复过程土壤酶活性的变化[J].水土保持研究,2014,21(6):95-100.QIAN Z,YU Y C,YU X P,et al. Changes of vegetation characteristics and soil properties in Mu Us sandy land by aerial seeding afforestation[J]. Journal of Central South University of Forestry&Technology,2014,21(6):95-100(in Chinese).
[27]余娜,刘济明,张超,等.不同沙生植被土壤酶活性分异特征研究[J].水土保持研究,2010,17(1):77-79.YU N,LIU J M,ZHANG C,et al. Study on the differentiation characteristics of different sandy vegetation soil enzyme activities[J].Research of Soil&Water Conservation,2010,17(1):77-79(in Chinese).
[28]姜凤岐,曾德慧,于占源.从恢复生态学视角透析防护林衰退及其防治对策:以章古台地区樟子松林为例[J].应用生态学报,2006,17(12):2229-2235.JIANG F Q,ZENG D H,YU Z Y. Decline of protective forest and its prevention strategies from viewpoint of restoration ecology:Taking Pinus sylvestris var. mongolica plantation in Zhanggutai as an example[J]. Chinese Journal of Applied Ecology,2006,17(12):2229-2235(in Chinese).
[29]王兵,刘国彬,薛芝,等.黄土丘陵区撂荒对土壤酶活性的影响[J].草地学报,2009,17(3):282-287.WANG B,LIU G B,XUE Z,et al. Effect of farmland abandonment on soil enzyme activities in Loess hilly region[J]. Acta Agrestia Sinica,2009,17(3):282-287(in Chinese).
[30]杨万勤,王开运.土壤酶研究动态与展望[J].应用与环境生物学报,2002,8(5):564-570.YANG W Q,WANG K Y. Advances on soil enzymology[J]. Chinese Journal of Applied&Environmental Biology,2002,8(5):564-570(in Chinese).
[31]杨万勤,宋光煜,韩玉萍.土壤生态学的理论体系及其研究领域[J].生态学杂志,2000,19(4):53-56.YANG W Q,SONG G Y,HAN Y P. Theoretical system and research fields of soil ecology[J]. Chinese Journal of Ecology,2000,19(4):53-56(in Chinese).
[32]杨涛,徐慧,方德华,等.樟子松林下土壤养分、微生物及酶活性的研究[J].土壤通报,2006,37(2):2253-2257.YANG T,XU H,FANG D H,et al. Soil nutrient,microorganism and enzyme activity in Pinus sylvestris var. mongolica forests[J]. Chinese Journal of Soil Science,2006,37(2):2253-2257(in Chinese).
[33]耿玉清,白翠霞,赵广亮,等.土壤磷酸酶活性及其与有机磷组分的相关性[J].北京林业大学学报,2008,30(S2):139-143.GENG Y Q,BAI C X,ZHAO G L,et al. Soil phosphatase activity and its correlation with composition of organic phosphorus[J]. Journal of Beijing Forestry University,2008,30(S2):139-143(in Chinese).
[34]王素娟,高丽,苏和,等.内蒙古库布齐沙地土壤蛋白酶初步研究[J].草业科学,2009,26(9):13-17.WANG S J,GAO L,SU H,et al. The preliminary research of soil prolease on Khobqi sand area of Inner Mongolia[J]. Pratacultural Science,2009,26(9):13-17(in Chinese).
[35]王灿,王德建,孙瑞娟.长期不同施肥方式下土壤酶活性与肥力因素的相关性[J].生态环境,2008,17(2):688-692.WANG C,WANG D J,SUN R J. The relationship between soil enzyme activities and soil nutrients by long-term fertilizer experiments[J].Ecology&Environment,2008,17(2):688-692(in Chinese).