历年生鲜乳中舒巴坦敏感β-内酰胺酶类药物检验考核及注意事项分析
|
摘要
β-内酰胺酶非法掺入生鲜乳可分解其中残留β-内酰胺类抗生素(青霉素、头孢菌素),使常规检测方法无法检出有抗奶。为检测奶牛是否用过β-内酰胺类抗生素,检测部门开展了β-内酰胺酶类药物检测工作;而为了提高质检单位对乳及乳制品中β-内酰胺酶类项目检测结果的准确性及检测技术水平,农业部畜牧业司开展了对有资质的质量检测中心β-内酰胺酶类检测项目考核。本文即对历年考核结果进行归纳总结,同时对检测中需要注意事项进行分析阐述,以期为今后质量检测单位顺利通过考核及其他性能实验室准确检测乳及乳制品中舒巴坦敏感β-内酰胺酶类药物打下基础。
β-lactamases is an illegal additive to raw milk which breaks down β-lactam antibiotics (penicillins and cephalosporins),so that antibiotics contained in milk cannot be detected by conventional detection methods. In order to detect whether dairy cows have been administered with β-lactam antibiotics,inspection and test centers perform the β-lactamase test to confirm safety of milk. The Animal Husbandry Department of Ministry of Agriculture have organized inspections of the β-lactamase testing project to improve the accuracy and the level of β-lactamase testing in milk and other dairy products. This article summarizes and analyses the results of the β-lactam tests performed over the last seven years,especially proposing related key points and notes to be careful with,which may be helpful for related laboratories to improve accuracy of β-lactamase test.
β-lactamases is an illegal additive to raw milk which breaks down β-lactam antibiotics (penicillins and cephalosporins),so that antibiotics contained in milk cannot be detected by conventional detection methods. In order to detect whether dairy cows have been administered with β-lactam antibiotics,inspection and test centers perform the β-lactamase test to confirm safety of milk. The Animal Husbandry Department of Ministry of Agriculture have organized inspections of the β-lactamase testing project to improve the accuracy and the level of β-lactamase testing in milk and other dairy products. This article summarizes and analyses the results of the β-lactam tests performed over the last seven years,especially proposing related key points and notes to be careful with,which may be helpful for related laboratories to improve accuracy of β-lactamase test.
引文
[1]王桂琴,杨萌萌,邢燕.宁夏地区奶牛乳房炎金黄色葡萄球菌耐药性分析[J].动物医学进展,2011,32(10):59-62.
[2]卜仕金,陈杖榴,冯淇辉.奶牛乳房炎的抗菌药物防治[J].兽药与饲料添加剂,1999,43:14-16.
[3]王冰,刘明春,吴聪明.呼和浩特地区奶牛乳房炎金黄色葡萄球菌的耐药性调查[J].中国兽医杂志,2007,43(3):30-32.
[4]雷方,周爽,苗虹.杯碟法和酶热仪法检测乳及乳制品中β-内酰胺酶类物质的比较[J].食品安全质量检测学报,2014,5(10):3260-3261.
[5]王慧李春梅程青海.原料乳质量问题及控制方法[J].科技信息,2007(18):9-10.
[6]周德刚,邓程君,卢春香.乳及乳制品中4种违禁添加物的检测方法[J].饲料检测,2009(8):41-42.
[7]Korycka-Dahl M,Richardson T,Bradley R L J.Use of microbial Beta-lactamase to destroy penicillin added to milk[J].J Dairy Sci,1985,68(8):1910-1916.
[8]Lee M Z,Richards on T.Preparation and characterization of immobilized Beta-lactamase for destruction of penicillin in milk[J].JDairy Sci,1987,70(10):2032-2039.
[9]郑颖,王震,范泉水,等.TEM-116超广谱内酰胺对牛奶中抗生素的清除[J].中国抗生素杂志,2008,33(2):122-124.
[10]王立平,蔡雪凤,曹宝森.乳及乳制品中检测方法的研究[J].食品工业科技,2010(9):355-360.
[11]王艳.乳及乳制品中舒巴坦敏感β-内酰胺酶类药物检验方法及探讨[J].现代畜牧兽医,2009(10):23-26.
[12]卫监督发[2009]21号文件全国打击违法添加非食用物质和滥用食品添加剂专项整治近期工作重点及要求[S].北京:中华人民共和国卫生部,2009.
[13]卫监督发[2009]44号文件乳及乳制品中舒巴坦敏感β-内酰胺酶类药物检验方法-杯碟法[S].北京:中华人民共和国卫生部公报,2009.
[14]吕媛.β-内酰胺酶研究进展[J].中国临床药理学杂志,2001,17,454-457.
[15]Livermore D M.Determinants of the activity of lactamase inhibitor combinations[J].J Antimicrob Chemother,1993,31(Suppl A):19-21.
[16]Frere J M.Quantitative relationship between sensitivity to lactam antibiotics and-lactamases production gram-negative bacteria-I steadstate treatments[J].J Biochem Pharmacol,1989,38:1415-1426.
[17]Fere J M,Joris B.Quantitative relationship between sensitivity to lactam antibiotics and-lactamases production gram-negative bacteria-Ⅱnon-steadstate treatment and progress curves[J].J Biochem Pharmacol,1989,38:1427-1433.
[18]Liver D M,Cor kill J E.Effects of CO_2 and pH on inhibition of TEM-1 and other-lactamases by penicillanic acid sulfone[J].J Antimicrob Agents Chemother,1992,236:1870-1876.
[19]Nikaido H.Role of permeability barrier in resistance to-lactam antibiotics[J].Pharmacol Ther,1985,27:197-231.
[20]张春辉,胡功政,朱沙.β-内酰胺酶抑制剂舒巴坦的研究进展[J].中兽医医药杂志,2004(1):19-20.
[21]王艳.生鲜牛乳中β-内酰胺酶的快速检测法与杯碟法的比较[J].现代畜牧兽医,2010(9):59-60.
[22]米自由,苏亮,何义刚,等.生鲜牛乳中β-内酰胺酶检测方法-杯碟法的优化及应用[J].中国兽医杂志,2010,46(1):68-70.
[23]周蕊,丁颢,周爽,等.我国部分地区市售巴氏杀菌乳中β-内酰胺酶含量调查[J].中国食品卫生杂志,2014,26(3):209-211.
[24]薛晓晶,李玲,金涌,等.杯碟法检测乳中的β-内酰胺酶[J].食品科学,2011,32(4):216-219.
[25]张立静,吴萌,黄仁录.牛奶中TEM1型β-内酰胺酶免疫胶体金试纸条的研制[J].中国兽医杂志,2011,47(5):67.
[26]Cui S H,Li J Y,Hu C Q,et al.Development of a method for the detection of beta-lactamases in milk samples[J].J AOAC Int,2007,90:1128-1132.
[2]卜仕金,陈杖榴,冯淇辉.奶牛乳房炎的抗菌药物防治[J].兽药与饲料添加剂,1999,43:14-16.
[3]王冰,刘明春,吴聪明.呼和浩特地区奶牛乳房炎金黄色葡萄球菌的耐药性调查[J].中国兽医杂志,2007,43(3):30-32.
[4]雷方,周爽,苗虹.杯碟法和酶热仪法检测乳及乳制品中β-内酰胺酶类物质的比较[J].食品安全质量检测学报,2014,5(10):3260-3261.
[5]王慧李春梅程青海.原料乳质量问题及控制方法[J].科技信息,2007(18):9-10.
[6]周德刚,邓程君,卢春香.乳及乳制品中4种违禁添加物的检测方法[J].饲料检测,2009(8):41-42.
[7]Korycka-Dahl M,Richardson T,Bradley R L J.Use of microbial Beta-lactamase to destroy penicillin added to milk[J].J Dairy Sci,1985,68(8):1910-1916.
[8]Lee M Z,Richards on T.Preparation and characterization of immobilized Beta-lactamase for destruction of penicillin in milk[J].JDairy Sci,1987,70(10):2032-2039.
[9]郑颖,王震,范泉水,等.TEM-116超广谱内酰胺对牛奶中抗生素的清除[J].中国抗生素杂志,2008,33(2):122-124.
[10]王立平,蔡雪凤,曹宝森.乳及乳制品中检测方法的研究[J].食品工业科技,2010(9):355-360.
[11]王艳.乳及乳制品中舒巴坦敏感β-内酰胺酶类药物检验方法及探讨[J].现代畜牧兽医,2009(10):23-26.
[12]卫监督发[2009]21号文件全国打击违法添加非食用物质和滥用食品添加剂专项整治近期工作重点及要求[S].北京:中华人民共和国卫生部,2009.
[13]卫监督发[2009]44号文件乳及乳制品中舒巴坦敏感β-内酰胺酶类药物检验方法-杯碟法[S].北京:中华人民共和国卫生部公报,2009.
[14]吕媛.β-内酰胺酶研究进展[J].中国临床药理学杂志,2001,17,454-457.
[15]Livermore D M.Determinants of the activity of lactamase inhibitor combinations[J].J Antimicrob Chemother,1993,31(Suppl A):19-21.
[16]Frere J M.Quantitative relationship between sensitivity to lactam antibiotics and-lactamases production gram-negative bacteria-I steadstate treatments[J].J Biochem Pharmacol,1989,38:1415-1426.
[17]Fere J M,Joris B.Quantitative relationship between sensitivity to lactam antibiotics and-lactamases production gram-negative bacteria-Ⅱnon-steadstate treatment and progress curves[J].J Biochem Pharmacol,1989,38:1427-1433.
[18]Liver D M,Cor kill J E.Effects of CO_2 and pH on inhibition of TEM-1 and other-lactamases by penicillanic acid sulfone[J].J Antimicrob Agents Chemother,1992,236:1870-1876.
[19]Nikaido H.Role of permeability barrier in resistance to-lactam antibiotics[J].Pharmacol Ther,1985,27:197-231.
[20]张春辉,胡功政,朱沙.β-内酰胺酶抑制剂舒巴坦的研究进展[J].中兽医医药杂志,2004(1):19-20.
[21]王艳.生鲜牛乳中β-内酰胺酶的快速检测法与杯碟法的比较[J].现代畜牧兽医,2010(9):59-60.
[22]米自由,苏亮,何义刚,等.生鲜牛乳中β-内酰胺酶检测方法-杯碟法的优化及应用[J].中国兽医杂志,2010,46(1):68-70.
[23]周蕊,丁颢,周爽,等.我国部分地区市售巴氏杀菌乳中β-内酰胺酶含量调查[J].中国食品卫生杂志,2014,26(3):209-211.
[24]薛晓晶,李玲,金涌,等.杯碟法检测乳中的β-内酰胺酶[J].食品科学,2011,32(4):216-219.
[25]张立静,吴萌,黄仁录.牛奶中TEM1型β-内酰胺酶免疫胶体金试纸条的研制[J].中国兽医杂志,2011,47(5):67.
[26]Cui S H,Li J Y,Hu C Q,et al.Development of a method for the detection of beta-lactamases in milk samples[J].J AOAC Int,2007,90:1128-1132.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |