数据融合快速鉴别9种野生食用牛肝菌
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摘要
食用菌种类与产地品质存在差异,加上因牟利产生的混杂销售现象,严重制约了高原特色农产品来源鉴别、种质资源评价和深入挖掘利用。本试验融合牛肝菌光谱信息建立支持向量机(SVM)模型,寻找最佳的样品种类鉴别方法。结果显示:(1)元素标准曲线R~2>0.999,RSD<5.0%,标准物回收率94%–106%,测定方法可靠;(2)样品含Ca、Na等人体必需元素,但Cd含量超标;(3)脂肪酸、蛋白质等化合物和Ni、Co等矿质元素对种类鉴别贡献最大;(4)中级数据融合优于低级数据融合,优于单一光谱数据模型。数据融合结合化学计量学可实现样品种类快速准确鉴别,对食用菌市场监督、种质资源评价及挖掘利用具有理论参考意义。
The prediction model of support vector machine(SVM) was established based on spectra and data fusion to find reliable approach for quality identification of marketing edible Boletus in Yunnan Province. The results indicated that element content calibration curve was R~2>0.999 and RSD<5.0%, and the standard recovery rate was 94%–106%. The marketing samples contain necessary elements of human body such as Ca,Na, etc., while Cd content exceeds the provided standard. Fatty acids, proteins, Ni, Co, etc. were the main substances for quality identification. Among all the models, mid-level data fusion was superior to low-level data fusion and single spectral data model. The rapid and accurate quality identification was achieved by means of data fusion combined with chemometrics, providing a theoretical reference for market supervision,germplasm resources evaluation and exploiting utilization potentiality of Boletus mushrooms.
The prediction model of support vector machine(SVM) was established based on spectra and data fusion to find reliable approach for quality identification of marketing edible Boletus in Yunnan Province. The results indicated that element content calibration curve was R~2>0.999 and RSD<5.0%, and the standard recovery rate was 94%–106%. The marketing samples contain necessary elements of human body such as Ca,Na, etc., while Cd content exceeds the provided standard. Fatty acids, proteins, Ni, Co, etc. were the main substances for quality identification. Among all the models, mid-level data fusion was superior to low-level data fusion and single spectral data model. The rapid and accurate quality identification was achieved by means of data fusion combined with chemometrics, providing a theoretical reference for market supervision,germplasm resources evaluation and exploiting utilization potentiality of Boletus mushrooms.
引文
Bajoub A,Medina-Rodríguez S,Cuadros-Rodríguez L,Monasterio PR,Vercammen J,Femandez-Gutierrez A,Carrasco-Pancorbo A,2018.A metabolic fingerprinting approach based on selected ion flow tube mass spectrometry(SIFT-MS)and chemometrics:a reliable tool for Mediterranean origin-labeled olive oils authentication.Food Research International,106:233-242
Biancolillo A,Bucci R,MagrìAL,Magri AD,Marini F,2014.Data-fusion for multiplatform characterization of an Italian craft beer aimed at its authentication.Analytica Chimica Acta,820:23-31
Borràs E,FerréJ,BoquéR,Mestres M,Acena L,Busto O,2015.Data fusion methodologies for food and beverage authentication and quality assessment-a review.Analytica Chimica Acta,891:1-14
Callao MP,Ruisánchez I,2018.An overview of multivariate qualitative methods for food fraud detection.Food Control,86:283-293
Chen JB,Sun SQ,Ma F,Zhou Q,2014.Vibrational microspectroscopic identification of powdered traditional medicines:chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,128:629-637
Cho IH,Kim YS,Choi HK,2007.Metabolomic discrimination of different grades of pine-mushroom(Tricholoma matsutake Sing.)using 1H NMR spectrometry and multivariate data analysis.Journal of Pharmaceutical and Biomedical Analysis,43(3):900-904
Cuevas FJ,Pereira-Caro G,Moreno-Rojas JM,Munoz-Redondo JM,Ruiz-Moreno MJ,2017.Assessment of premium organic orange juices authenticity using HPLC-HR-MS and HS-SPME-GC-MS combining data fusion and chemometrics.Food Control,82:203-211
Falandysz J,Zhang J,Wiejak J,Baralkiewicz A,Hanc D,Anetta,2017.Metallic elements and metalloids in Boletus luridus,B.magnificus and B.tomentipes mushrooms from polymetallic soils from SW China.Ecotoxicology and Environmental Safety,142:497-502
Fischer G,Braun S,Thissen R,Dott W,2006.FT-IRspectroscopy as a tool for rapid identification and intra-species characterization of airborne filamentous fungi.Journal of Microbiological Methods,64(1):63-67
Hindu SV,Thanigaivel S,Vijayakumar S,Chandrasekaran N,Mukherjee A,Thomas J,2018.Effect of microencapsulated probiotic Bacillus vireti01-polysaccharide extract of Gracilaria folifera with alginate-chitosan on immunity,antioxidant activity and disease resistance of Macrobrachium rosenbergii against Aeromonas hydrophila infection.Fish&Shellfish Immunology,73:112-120
Hu YY,Wang T,Yang XB,Zhao Y,2014.Analysis of compositional monosaccharides in fungus polysaccharides by capillary zone electrophoresis.Carbohydrate Polymers,102:481-488
Jaeger BN,Parylak SL,Gage FH,2017.Mechanisms of dietary flavonoid action in neuronal function and neuroinflammation.Molecular Aspects of Medicine,61:50-62
Li Y,Zhang J,Li T,Yang TW,Wang YZ,Liu HG,2016.Ultraviolet spectroscopy used to fingerprint five wild-grown edible mushrooms(Boletaceae)collected from Yunnan,China.Journal of Spectroscopy.http://dx.doi.org/10.1155/2016/7813405
Li Y,Zhang J,Zhao Y,Li ZM,Li T,Wang YZ,2014.Characteristic fingerprint based on low polar constituents for discrimination of Wolfiporia extensa according to geographical origin using UV spectroscopy and chemometrics methods.Journal of Analytical Methods in Chemistry.http://dx.doi.org/10.1155/2014/519424
Li Y,Zhang JY,Wang YZ,2018.FT-MIR and NIR spectral data fusion:a synergetic strategy for the geographical traceability of Panax notoginseng.Analytical and Bioanalytical Chemistry,410(1):91-103
Liu B,Huang Q,Cai H,Xiang G,Ting W,Mingying G,2015.Study of heavy metal concentrations in wild edible mushrooms in Yunnan Province,China.Food Chemistry,188:294-300
Liu JY,Ying ZH,Liu F,Liu XR,Xie BG,2012.Evaluation of the use of SCAR markers for screening genetic diversity of Lentinula edodes strains.Current Microbiology,64(4):317-325
Mellado-Mojica E,López MG,2015.Identification,classification,and discrimination of Agave syrups from natural sweeteners by infrared spectroscopy and HPAEC-PAD.Food Chemistry,167:349-357
Moha?ek-Gro?ev V,Bo?ac R,Puppels GJ,2001.Vibrational spectroscopic characterization of wild growing mushrooms and toadstools.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,57(14):2815-2829
Obisesan KA,Jiménez-Carvelo AM,Cuadros-Rodriguez L,Ruisanchez L,Callao MP,2017.HPLC-UV and HPLC-CADchromatographic data fusion for the authentication of the geographical origin of palm oil.Talanta,170:413-418
O’Gorman A,Downey G,Gowen AA,Barry-Ryant C,Frias JM,2010.Use of Fourier transform infrared spectroscopy and chemometric data analysis to evaluate damage and age in mushrooms(Agaricus bisporus)grown in Ireland.Journal of Agricultural and Food Chemistry,58(13):7770-7776
Pande R,Mishra HN,2015.Fourier transform near-infrared spectroscopy for rapid and simple determination of phytic acid content in green gram seeds(Vigna radiata).Food Chemistry,172:880-884
Qi LM,Liu HG,Li JQ,Li T,Wang YZ,2018.Feature fusion of ICP-AES,UV-Vis and FT-MIR for origin traceability of Boletus edulis mushrooms in combination with chemometrics.Sensors,18(1):241-256
Sun SQ,Zhang Q,Chen JB,2017.Analysis of traditional Chinese medicine by infrared spectroscopy.Chemical Industry Press,Beijing.1-358(in Chinese)
Wang XM,Zhang J,Wu LH,Zhao YL,Li T,Li JQ,Wang YZ,2014.A mini-review of chemical composition and nutritional value of edible wild-grown mushroom from China.Food Chemistry,151:279-285
Wen H,Kang S,Song Y,Song SH,Park S,2010.Differentiation of cultivation sources of Ganoderma lucidum by NMR-based metabolomics approach.Phytochemical Analysis,21(1):73-79
Yang TW,Li T,Zhang J,Li JQ,Liu HG,Wang YZ,2015a.Ultraviolet spectrum identification of bolete mushrooms from different regions and species.The Food Industry,36(9):208-212(in Chinese)
Yang TW,Zhang J,Shi YD,Li T,Wang YZ,Liu HG,2015b.Infrared spectroscopy combined with multivariate statistical analysis to quickly identify different species of bolete mushrooms.Food Science,36(24):116-121(in Chinese)
Yao S,Li JQ,Li T,Liu HG,Wang YZ,2018a.Discrimination of Boletaceae mushrooms based on data fusion of FT-IRand ICP-AES combined with SVM.International Journal of Food Properties,21(1):270-281
Yao S,Li T,Li JQ,Liu HG,Wang YZ,2018b.Geographic identification of Boletus mushrooms by data fusion of FT-IR and UV spectroscopies combined with multivariate statistical analysis.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,198:257-263
Zervakis GI,Bekiaris G,Tarantilis PΑ,Pappas CS,2012.Rapid strain classification and taxa delimitation within the edible mushroom genus Pleurotus through the use of diffuse reflectance infrared fourier transform(DRIFT)spectroscopy.Fungal Biology,116(6):715-728
孙素琴,张群,陈建波,2017.中药红外光谱分析与鉴定.北京:化学工业出版社.1-358
杨天伟,李涛,张霁,李杰庆,刘鸿高,王元忠,2015a.不同产地、种类牛肝菌的紫外光谱鉴别分析.食品工业,36(9):208-212
杨天伟,张霁,史云东,李涛,王元忠,刘鸿高,2015b.红外光谱结合多元统计分析快速鉴别不同种类牛肝菌.食品科学,36(24):116-121
Biancolillo A,Bucci R,MagrìAL,Magri AD,Marini F,2014.Data-fusion for multiplatform characterization of an Italian craft beer aimed at its authentication.Analytica Chimica Acta,820:23-31
Borràs E,FerréJ,BoquéR,Mestres M,Acena L,Busto O,2015.Data fusion methodologies for food and beverage authentication and quality assessment-a review.Analytica Chimica Acta,891:1-14
Callao MP,Ruisánchez I,2018.An overview of multivariate qualitative methods for food fraud detection.Food Control,86:283-293
Chen JB,Sun SQ,Ma F,Zhou Q,2014.Vibrational microspectroscopic identification of powdered traditional medicines:chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,128:629-637
Cho IH,Kim YS,Choi HK,2007.Metabolomic discrimination of different grades of pine-mushroom(Tricholoma matsutake Sing.)using 1H NMR spectrometry and multivariate data analysis.Journal of Pharmaceutical and Biomedical Analysis,43(3):900-904
Cuevas FJ,Pereira-Caro G,Moreno-Rojas JM,Munoz-Redondo JM,Ruiz-Moreno MJ,2017.Assessment of premium organic orange juices authenticity using HPLC-HR-MS and HS-SPME-GC-MS combining data fusion and chemometrics.Food Control,82:203-211
Falandysz J,Zhang J,Wiejak J,Baralkiewicz A,Hanc D,Anetta,2017.Metallic elements and metalloids in Boletus luridus,B.magnificus and B.tomentipes mushrooms from polymetallic soils from SW China.Ecotoxicology and Environmental Safety,142:497-502
Fischer G,Braun S,Thissen R,Dott W,2006.FT-IRspectroscopy as a tool for rapid identification and intra-species characterization of airborne filamentous fungi.Journal of Microbiological Methods,64(1):63-67
Hindu SV,Thanigaivel S,Vijayakumar S,Chandrasekaran N,Mukherjee A,Thomas J,2018.Effect of microencapsulated probiotic Bacillus vireti01-polysaccharide extract of Gracilaria folifera with alginate-chitosan on immunity,antioxidant activity and disease resistance of Macrobrachium rosenbergii against Aeromonas hydrophila infection.Fish&Shellfish Immunology,73:112-120
Hu YY,Wang T,Yang XB,Zhao Y,2014.Analysis of compositional monosaccharides in fungus polysaccharides by capillary zone electrophoresis.Carbohydrate Polymers,102:481-488
Jaeger BN,Parylak SL,Gage FH,2017.Mechanisms of dietary flavonoid action in neuronal function and neuroinflammation.Molecular Aspects of Medicine,61:50-62
Li Y,Zhang J,Li T,Yang TW,Wang YZ,Liu HG,2016.Ultraviolet spectroscopy used to fingerprint five wild-grown edible mushrooms(Boletaceae)collected from Yunnan,China.Journal of Spectroscopy.http://dx.doi.org/10.1155/2016/7813405
Li Y,Zhang J,Zhao Y,Li ZM,Li T,Wang YZ,2014.Characteristic fingerprint based on low polar constituents for discrimination of Wolfiporia extensa according to geographical origin using UV spectroscopy and chemometrics methods.Journal of Analytical Methods in Chemistry.http://dx.doi.org/10.1155/2014/519424
Li Y,Zhang JY,Wang YZ,2018.FT-MIR and NIR spectral data fusion:a synergetic strategy for the geographical traceability of Panax notoginseng.Analytical and Bioanalytical Chemistry,410(1):91-103
Liu B,Huang Q,Cai H,Xiang G,Ting W,Mingying G,2015.Study of heavy metal concentrations in wild edible mushrooms in Yunnan Province,China.Food Chemistry,188:294-300
Liu JY,Ying ZH,Liu F,Liu XR,Xie BG,2012.Evaluation of the use of SCAR markers for screening genetic diversity of Lentinula edodes strains.Current Microbiology,64(4):317-325
Mellado-Mojica E,López MG,2015.Identification,classification,and discrimination of Agave syrups from natural sweeteners by infrared spectroscopy and HPAEC-PAD.Food Chemistry,167:349-357
Moha?ek-Gro?ev V,Bo?ac R,Puppels GJ,2001.Vibrational spectroscopic characterization of wild growing mushrooms and toadstools.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,57(14):2815-2829
Obisesan KA,Jiménez-Carvelo AM,Cuadros-Rodriguez L,Ruisanchez L,Callao MP,2017.HPLC-UV and HPLC-CADchromatographic data fusion for the authentication of the geographical origin of palm oil.Talanta,170:413-418
O’Gorman A,Downey G,Gowen AA,Barry-Ryant C,Frias JM,2010.Use of Fourier transform infrared spectroscopy and chemometric data analysis to evaluate damage and age in mushrooms(Agaricus bisporus)grown in Ireland.Journal of Agricultural and Food Chemistry,58(13):7770-7776
Pande R,Mishra HN,2015.Fourier transform near-infrared spectroscopy for rapid and simple determination of phytic acid content in green gram seeds(Vigna radiata).Food Chemistry,172:880-884
Qi LM,Liu HG,Li JQ,Li T,Wang YZ,2018.Feature fusion of ICP-AES,UV-Vis and FT-MIR for origin traceability of Boletus edulis mushrooms in combination with chemometrics.Sensors,18(1):241-256
Sun SQ,Zhang Q,Chen JB,2017.Analysis of traditional Chinese medicine by infrared spectroscopy.Chemical Industry Press,Beijing.1-358(in Chinese)
Wang XM,Zhang J,Wu LH,Zhao YL,Li T,Li JQ,Wang YZ,2014.A mini-review of chemical composition and nutritional value of edible wild-grown mushroom from China.Food Chemistry,151:279-285
Wen H,Kang S,Song Y,Song SH,Park S,2010.Differentiation of cultivation sources of Ganoderma lucidum by NMR-based metabolomics approach.Phytochemical Analysis,21(1):73-79
Yang TW,Li T,Zhang J,Li JQ,Liu HG,Wang YZ,2015a.Ultraviolet spectrum identification of bolete mushrooms from different regions and species.The Food Industry,36(9):208-212(in Chinese)
Yang TW,Zhang J,Shi YD,Li T,Wang YZ,Liu HG,2015b.Infrared spectroscopy combined with multivariate statistical analysis to quickly identify different species of bolete mushrooms.Food Science,36(24):116-121(in Chinese)
Yao S,Li JQ,Li T,Liu HG,Wang YZ,2018a.Discrimination of Boletaceae mushrooms based on data fusion of FT-IRand ICP-AES combined with SVM.International Journal of Food Properties,21(1):270-281
Yao S,Li T,Li JQ,Liu HG,Wang YZ,2018b.Geographic identification of Boletus mushrooms by data fusion of FT-IR and UV spectroscopies combined with multivariate statistical analysis.Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,198:257-263
Zervakis GI,Bekiaris G,Tarantilis PΑ,Pappas CS,2012.Rapid strain classification and taxa delimitation within the edible mushroom genus Pleurotus through the use of diffuse reflectance infrared fourier transform(DRIFT)spectroscopy.Fungal Biology,116(6):715-728
孙素琴,张群,陈建波,2017.中药红外光谱分析与鉴定.北京:化学工业出版社.1-358
杨天伟,李涛,张霁,李杰庆,刘鸿高,王元忠,2015a.不同产地、种类牛肝菌的紫外光谱鉴别分析.食品工业,36(9):208-212
杨天伟,张霁,史云东,李涛,王元忠,刘鸿高,2015b.红外光谱结合多元统计分析快速鉴别不同种类牛肝菌.食品科学,36(24):116-121
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