摘要
为研究扩展莫尼茨绦虫分离株核糖体ITS序列的遗传变异情况,并利用ITS序列探讨扩展莫尼茨绦虫与其它带科绦虫的种群发育关系,本研究利用PCR对扩展莫尼茨绦虫分离株核糖体ITS序列进行扩增、测序及分析。结果表明:16株扩展莫尼茨绦虫分离株核糖体ITS序列长度一致,为1 462~1 473 bp,且分离株与基因库扩展莫尼茨绦虫位于同一大分支,可以与其它带科绦虫有效鉴别。说明ITS序列种内较为保守,种间差异较大,可以作为扩展莫尼茨绦虫的种间遗传变异研究的分子标记。
To analyze sequence variations in the ribosomal inter-transcribed spacer( ITS) among M.expansa isolates in Hunan Province,and explore phylogentic relationship between M. expansa and other tapeworm,the ITS regions of ribosomal DNA of M.expansa obtained were amplified by PCR,sequenced and analyzed. The results showed that the lengths of ITS sequences of 16 M.expansas isolates were 1 462-1 473 bp. The constructed phylogenetic tree based on ITS gene sequences revealed that the Hunan isolates and the M.expansa available in GenBank were clustered in the same clade. It is concluded that ITS sequences could be used as a genetic marker for interspecies genetic variation research of M.expansa.
引文
[1]付文龙,陈冬,朱萍,等.吡喹酮和芬苯咪唑对圈养林麝莫尼茨绦虫的驱除试验[J].经济动物学报,2016,20(3):156-158.
[2]吕向辉,乔继英,吴晓民,等.主要危害麝类的化脓性、呼吸系统疾病及寄生虫病研究[J].经济动物学报,2009,13(2):104-107.
[3]王开胜,刘继荣,薄新文.贝氏莫尼茨绦虫与扩展莫尼茨绦虫的形态学比较[J].新疆农业科学,2010,47(3):612-618.
[4]张秋雨,刘志科,银梅.一起羔羊莫尼茨绦虫病的中西结合疗法及体会[J].黑龙江畜牧兽医,2016(8):145-146.
[5]阎晓菲,韩冰,黄兵,等.羊消化道寄生虫种类及防治措施[J].黑龙江畜牧兽医,2014(16):63-66.
[6]哈希巴特,陈千林,许正茂,等.新疆巴州牦牛消化道寄生虫感染的现状[J].畜牧与兽医,2016,48(1):113-115.
[7]贺飞飞,李春华,雷萌桐,等.青海省海南州兴海县牦牛寄生虫病流行情况调查[J].畜牧与兽医,2015,47(2):107-111.
[8]谭磊,王先坤,周湘,等.多头带绦虫湖南分离株核糖体18S基因系统进化分析[J].经济动物学报,2016,20(2):89-93.
[9]Liu W,Liu G H.Sequence variability in three mitochondrial DNA regions of Spirometra erinaceieuropaei spargana of human and animal health significance[J].J Helminthol,2012,86(3):271-275.
[10]Dai R S,Liu G H.Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China[J].J Helminthol,2012,86(2):245-251.