橡胶树HbDAHPS基因过表达对拟南芥抗逆性的影响
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摘要
莽草酸途径与植物的抗逆相关,研究莽草酸途径中的关键酶,有助于了解莽草酸途径在整个植物抗逆过程中的作用机制。3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合成酶(DAHPS)是莽草酸途径七个酶促反应中的第一个关键酶,在控制分支酸的合成中有着十分重要的作用,为明确橡胶树HbDAHPSS在响应非生物胁迫中的功能,将HbDAHPSS在拟南芥中进行了过表达,对单拷贝转基因株系进行低温、干旱、盐害等胁迫处理,结果表明,橡胶树HbDAHPSS的过表达增强了拟南芥植株的抗旱性、抗盐性和抗寒性。
Shikimate pathway is associated with plant resistance. The study of key enzymes in shikimate pathway is helpful to understand the mechanism of plant stress resistance. The main trunk of shikimate pathway consists of seven catalyzed reactions. 3-Deoxy-d-arabino-heptulosonate-7-phosphate synthetase(DAHPS) is the key enzyme which catalyzes the first reaction in shikimate pathway, playing a vital role in controlling chorismate biosynthesis.In order to investigate the function of HbDAHPSS under abiotic stress, HbDAHPSS was overexpressed in Arabidopsis thaliana. The transgenic plants were analyzed under drought, salt and cold stress, respectively. All of the results showed that the overexpression of HbDAHPSS enhanced drought, salt and cold resistance in Arabidopsis.
Shikimate pathway is associated with plant resistance. The study of key enzymes in shikimate pathway is helpful to understand the mechanism of plant stress resistance. The main trunk of shikimate pathway consists of seven catalyzed reactions. 3-Deoxy-d-arabino-heptulosonate-7-phosphate synthetase(DAHPS) is the key enzyme which catalyzes the first reaction in shikimate pathway, playing a vital role in controlling chorismate biosynthesis.In order to investigate the function of HbDAHPSS under abiotic stress, HbDAHPSS was overexpressed in Arabidopsis thaliana. The transgenic plants were analyzed under drought, salt and cold stress, respectively. All of the results showed that the overexpression of HbDAHPSS enhanced drought, salt and cold resistance in Arabidopsis.
引文
Ding C.,Yang Q.H.,Li F.S.,and Lin W.F.,2006,Effect of cold stress on the content of free-proline in leaf of Saccharum spontaneum L.and sclerostachya,Anhui Nongye Kexu(Journal of Anhui Agricultural Sciences),34(5):846-849(丁灿,杨清辉,李富生,林位夫,2006,低温胁迫对割手密和斑茅游离脯氨酸含量的影响(Ⅱ),安徽农业科学,34(5)846-849)
Durner J.,Shah J.,and Klessig D.F.,1999,Salicylic acid and dis ease resistance in plant,Trends in Plant Science,2(7):266-274
Gorlach J.,Beck A.,Henstrand J.M.,Handa A.K.,Herrmann KM.,Schmid J.,and Amrhein N.,1993,Differential expres sion of tomato(Lycopersicon esculentum L.)genes encoding shikimate pathway isoenzymes.I.3-deoxy-D-a rabino-hep tulosonate 7-phosphate synthase,Plant Mol.Biol.23(4):697-706
He S.L.,Zheng J.G.,Wang X.F.,Wang Y.H.,Xu M.,Li B.L.,and Lin M.,2002,Plant secondary metabolism:function,regulation and gene engineering,Yingyong Yu Huanjing Shengwu Xuebao(Chinese Journal of Applied and Environmental Biology),8(5):558-563(何水林,郑金贵,王晓峰,王燕华,许明,李斌莲,林明,2002,植物次生代谢:功能、调控及其基因工程,应用与环境生物学报,8(5):558-563)
Herrmann K.M.,1995,The shikimate pathway as an entry to aromatic secondary metabolism,Plant Physiol.,107(1):7-12
Heyes L.C.,Reichau S.,Cross P.J.,Jameson G.B.,and Parker E.J.,2014,Structural analysis of substrate-mimicking inhibitors in complex with Neisseria meningitidis 3-deoxy-D-arabino-heptulosonate 7-phosphatesynthase-The importance of accommodating the active site water,Bioorganic Chemistry,57:242-250
Holmberg N.,Farrés J.,Bailey J.E.,and Kallio P.T.,2001,Targeted expression of a synthetic codon optimized gene,encoding the spruce budworm antifreeze protein,leads to accumulation of antifreeze activity in the apoplasts of transgenic tobacco,Gene,275(1):115-124
Huisman O.C.,and Kosuge T.,1974,Regulation of aromatic amino acid biosynthesis in higher plants:3-Deoxy-D-arabino-heptulosonate 7-phosphate synthetase from cauli flower,J.Biol.Chem.,249:6842-6848
Ingrain J.,and Bartels D.,1996,The molecular basis of dedydration tolerance in plants,Annual Review of Plant Physiology and Plant Molecular Biology,47:377-403
Liu C.Y.,Meng Q.M.,and Quan M.Q.,1993,Studies on some Physiological changes of eichhorina crassipes under Chilling injury,Wuhan Zhiwuxue Yanjiu(Journal of Wuhan Botani cal Research),11(4):345-353(刘成运,孟庆梅,权明清,1993,冷害条件下凤眼莲某些生理特性变化的研究,武汉植物学研究,11(4):345-353)
Liu Y.,Zhao H.C.,Li X.,and Wang L.X.,2002,Dynamics of starch grain sugar materials and lipids on pear shoots and its relationship to cold resistance,Neimenggu Nongye Daxue Xuebao(Journal of Inner Mongolia Agricultural University),23(1):51-57(刘艳,赵虎成,李雄,王丽雪,2002,梨枝条中淀粉、还原糖及脂类物质动态变化与抗寒性的关系,内蒙古农业大学学报,23(1):51-57)
Oh M.M.,Trick H.N.,and Rajashekar C.B.,2009,Secondary metabolism and antioxidants are involved in environmental adaption and stress tolerance in lettuce,Journal of Plant Physiology,166(2):180-191
Parvanova D.,Popova A.,Zaharieva I.,Lambrev P.,Konstantinova T.,Taneva S.,Atanassov A.,Goltsev V.,and Djilianov D.,2004,Low temperature tolerance of tobacco pl ants transformed to accumulate proline,fructans,or glycine be taine,variable chlorophyll fluorescence evidence,Photosyn thetica,42(2):179-185
Rippert P.,Scimemi C.,Dubald M.,and Matringe M.,2004,En gineering plant shikimate pathway for production of to cotrienol and improving herbicide resistance,Plant Physiol.134(1):92-100
Pinto J.E.,Suzlch J.A.,and Herrmann K.M.,1986,3-Deoxy-Darabino-heptulosonate 7-phosphate synthase from potato tu ber(Solanum tubemsum L.),Plant Physiol.,82(4):1040-1044
Rubin J.L.,Galnes C.G.,and Jensen R.A.,1982,Enzymologica basis for herbicidal action of glyphosate,Plant Physiol.,70833-839
Rvero R.M.,Ruiz J.M.,García P.C.,López-Lefebre L.R.Sánchez E.,and Romero L.,2001,Resistance to cold and heat stress:accumulation of phenolic compounds in tomato and watermelon plants,Plant Science,160(2):315-321
Sha Q.,Jiang M.Y.,Lin F.,and Wang J.X.,2009,The expression of calmodulin genes induced by water stress is associated with ABA and H2O2,Nanjing Nongye Daxue Xuebao(Jour nal of Nanjing Agricultural University),32(3):52-57(沙琴蒋明义,林凡,王金香,2009,水分胁迫诱导的玉米叶片钙调素基因表达及其与ABA和H2O2的关系,南京农业大学学报,32(3):52-57)
Suzich J.A.,Dean J.F.D.,and Herrmann K.M.,1985,3-deoxy-Darabino-heptulosonate 7-phosphate synthase from carrot roo(Daucus carota)is a hysteretic enzyme,Plant Physiol.,79(3)765-70
Thomashow M.F.,1999,Plant cold acclimation:freezing toler ance genes and regulatory mechanisms,Annual Review in Plant Physiology and Plant Molecular Biology,50:571-599
Troll W.,and Lindsley J.,1955,A photometric method for th determination of proline,J.Biol.Chem.,215(2):655-660
Tzin V.,and Galili G.,2010,New insights into the shikimate and aromatic amino acids biosynthesis pathways in plants Molecular Plant,3(6):956-972
Xiong L.,and Zhu J.K.,2001,Abiotic stress signal transduction in plants:molecular and genetic perspective,Physiologi Plantarum,112(2):152-166
Zhang D.Z.,Wang P.H.,and Zhao H.X.,1990,Determination o the content of free proline in wheat leaves,Zhiwu Shenglixu Tongxun(Plant Physiology Communications),(4):62-65(张殿忠,汪沛洪,赵会贤,1990,测定小麦叶片游离脯氨酸含量的方法,植物生理学通讯,(4):62-65)
Zhang N.,and Qin Z.W.,2007,Effects of low temperature on physio-biochemical index in spinach,Zhongguo Shuca(China Vegetables),(11):22-24(张南,秦智伟,2007,低温处理对菠菜生理生化指标的影响,中国蔬菜,(11):22-24)
Durner J.,Shah J.,and Klessig D.F.,1999,Salicylic acid and dis ease resistance in plant,Trends in Plant Science,2(7):266-274
Gorlach J.,Beck A.,Henstrand J.M.,Handa A.K.,Herrmann KM.,Schmid J.,and Amrhein N.,1993,Differential expres sion of tomato(Lycopersicon esculentum L.)genes encoding shikimate pathway isoenzymes.I.3-deoxy-D-a rabino-hep tulosonate 7-phosphate synthase,Plant Mol.Biol.23(4):697-706
He S.L.,Zheng J.G.,Wang X.F.,Wang Y.H.,Xu M.,Li B.L.,and Lin M.,2002,Plant secondary metabolism:function,regulation and gene engineering,Yingyong Yu Huanjing Shengwu Xuebao(Chinese Journal of Applied and Environmental Biology),8(5):558-563(何水林,郑金贵,王晓峰,王燕华,许明,李斌莲,林明,2002,植物次生代谢:功能、调控及其基因工程,应用与环境生物学报,8(5):558-563)
Herrmann K.M.,1995,The shikimate pathway as an entry to aromatic secondary metabolism,Plant Physiol.,107(1):7-12
Heyes L.C.,Reichau S.,Cross P.J.,Jameson G.B.,and Parker E.J.,2014,Structural analysis of substrate-mimicking inhibitors in complex with Neisseria meningitidis 3-deoxy-D-arabino-heptulosonate 7-phosphatesynthase-The importance of accommodating the active site water,Bioorganic Chemistry,57:242-250
Holmberg N.,Farrés J.,Bailey J.E.,and Kallio P.T.,2001,Targeted expression of a synthetic codon optimized gene,encoding the spruce budworm antifreeze protein,leads to accumulation of antifreeze activity in the apoplasts of transgenic tobacco,Gene,275(1):115-124
Huisman O.C.,and Kosuge T.,1974,Regulation of aromatic amino acid biosynthesis in higher plants:3-Deoxy-D-arabino-heptulosonate 7-phosphate synthetase from cauli flower,J.Biol.Chem.,249:6842-6848
Ingrain J.,and Bartels D.,1996,The molecular basis of dedydration tolerance in plants,Annual Review of Plant Physiology and Plant Molecular Biology,47:377-403
Liu C.Y.,Meng Q.M.,and Quan M.Q.,1993,Studies on some Physiological changes of eichhorina crassipes under Chilling injury,Wuhan Zhiwuxue Yanjiu(Journal of Wuhan Botani cal Research),11(4):345-353(刘成运,孟庆梅,权明清,1993,冷害条件下凤眼莲某些生理特性变化的研究,武汉植物学研究,11(4):345-353)
Liu Y.,Zhao H.C.,Li X.,and Wang L.X.,2002,Dynamics of starch grain sugar materials and lipids on pear shoots and its relationship to cold resistance,Neimenggu Nongye Daxue Xuebao(Journal of Inner Mongolia Agricultural University),23(1):51-57(刘艳,赵虎成,李雄,王丽雪,2002,梨枝条中淀粉、还原糖及脂类物质动态变化与抗寒性的关系,内蒙古农业大学学报,23(1):51-57)
Oh M.M.,Trick H.N.,and Rajashekar C.B.,2009,Secondary metabolism and antioxidants are involved in environmental adaption and stress tolerance in lettuce,Journal of Plant Physiology,166(2):180-191
Parvanova D.,Popova A.,Zaharieva I.,Lambrev P.,Konstantinova T.,Taneva S.,Atanassov A.,Goltsev V.,and Djilianov D.,2004,Low temperature tolerance of tobacco pl ants transformed to accumulate proline,fructans,or glycine be taine,variable chlorophyll fluorescence evidence,Photosyn thetica,42(2):179-185
Rippert P.,Scimemi C.,Dubald M.,and Matringe M.,2004,En gineering plant shikimate pathway for production of to cotrienol and improving herbicide resistance,Plant Physiol.134(1):92-100
Pinto J.E.,Suzlch J.A.,and Herrmann K.M.,1986,3-Deoxy-Darabino-heptulosonate 7-phosphate synthase from potato tu ber(Solanum tubemsum L.),Plant Physiol.,82(4):1040-1044
Rubin J.L.,Galnes C.G.,and Jensen R.A.,1982,Enzymologica basis for herbicidal action of glyphosate,Plant Physiol.,70833-839
Rvero R.M.,Ruiz J.M.,García P.C.,López-Lefebre L.R.Sánchez E.,and Romero L.,2001,Resistance to cold and heat stress:accumulation of phenolic compounds in tomato and watermelon plants,Plant Science,160(2):315-321
Sha Q.,Jiang M.Y.,Lin F.,and Wang J.X.,2009,The expression of calmodulin genes induced by water stress is associated with ABA and H2O2,Nanjing Nongye Daxue Xuebao(Jour nal of Nanjing Agricultural University),32(3):52-57(沙琴蒋明义,林凡,王金香,2009,水分胁迫诱导的玉米叶片钙调素基因表达及其与ABA和H2O2的关系,南京农业大学学报,32(3):52-57)
Suzich J.A.,Dean J.F.D.,and Herrmann K.M.,1985,3-deoxy-Darabino-heptulosonate 7-phosphate synthase from carrot roo(Daucus carota)is a hysteretic enzyme,Plant Physiol.,79(3)765-70
Thomashow M.F.,1999,Plant cold acclimation:freezing toler ance genes and regulatory mechanisms,Annual Review in Plant Physiology and Plant Molecular Biology,50:571-599
Troll W.,and Lindsley J.,1955,A photometric method for th determination of proline,J.Biol.Chem.,215(2):655-660
Tzin V.,and Galili G.,2010,New insights into the shikimate and aromatic amino acids biosynthesis pathways in plants Molecular Plant,3(6):956-972
Xiong L.,and Zhu J.K.,2001,Abiotic stress signal transduction in plants:molecular and genetic perspective,Physiologi Plantarum,112(2):152-166
Zhang D.Z.,Wang P.H.,and Zhao H.X.,1990,Determination o the content of free proline in wheat leaves,Zhiwu Shenglixu Tongxun(Plant Physiology Communications),(4):62-65(张殿忠,汪沛洪,赵会贤,1990,测定小麦叶片游离脯氨酸含量的方法,植物生理学通讯,(4):62-65)
Zhang N.,and Qin Z.W.,2007,Effects of low temperature on physio-biochemical index in spinach,Zhongguo Shuca(China Vegetables),(11):22-24(张南,秦智伟,2007,低温处理对菠菜生理生化指标的影响,中国蔬菜,(11):22-24)