进水NO_2~--N浓度对厌氧氨氧化反应器的影响
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摘要
为研究进水NO_2~--N浓度对厌氧氨氧化反应器的影响,以实验室培养的厌氧氨氧化菌为接种菌种,采用上流式厌氧氨氧化反应器,考察了进水中NO_2~--N浓度对厌氧氨氧化菌增殖速度、固碳量、微生物的变化情况的影响,发现进水亚硝酸盐氮对厌氧氨氧化反应器有很大的影响。当进水ρ(NH_4~+-N)、ρ(NO_2~--N)约为41. 23,51. 63 mg/L时,厌氧氨氧化菌的增殖速度最快为1. 7 mL/d,ρ(TS)为94. 03 g/L,最大比厌氧氨氧化活性(SAA)为0. 31 g/(g·d),去除率最高为91. 18%,固碳量为0. 71 mg/mg(N),微生物的丰富度最高,优势菌种Candidatus kuenenia基本未发生改变。若将进水ρ(NO_2~--N)增大为102. 99 mg/L时,Candidatus kuenenia丰度大量减少,厌氧氨氧化反应过程受到抑制,活性降低,厌氧氨氧化菌生长速度变慢。
In order to study the effect of influent nitrite nitrogen concentration on the anaerobic ammonium oxidation reactor,the laboratory cultured anaerobic ammonium oxidizing bacteria were used as inoculum,and up-flow anaerobic ammonium oxidation reactor was used to investigate the influence of influent nitrite nitrogen concentration on change conditions of proliferation rate,carbon sequestration,and microorganism of anaerobic ammonium oxidizing bacteria. It was found that the influent nitrite nitrogen had a great influence on the anaerobic ammonium oxidation reactor. When the influent NH_4~+-N and NO_2~--N concentrations were about 41. 23 mg/L and 51. 63 mg/L namely,the maximum proliferation rate of anaerobic ammoniac was 1. 7 mL/d,the concentration of TS was 94. 03 g/L,the maximum specific anammox activity( SAA) was 0. 31 g/( g·d),the maximum removal efficiency was 91. 18%,the fixed carbon content was 0. 71 mg/mg( N),the microbial richness was the highest,and the dominant strain Candidatus kuenenia was basically unchanged. When the influent NO_2~--N concentration was increased to 102. 99 mg/L,the dominant strain Candidatus kuenenia was significantly reduced,the anaerobic ammonium oxidation reaction process was inhibited,the activity was decreased,and the growth rate of anaerobic ammonium oxidizing bacteria became slower.
In order to study the effect of influent nitrite nitrogen concentration on the anaerobic ammonium oxidation reactor,the laboratory cultured anaerobic ammonium oxidizing bacteria were used as inoculum,and up-flow anaerobic ammonium oxidation reactor was used to investigate the influence of influent nitrite nitrogen concentration on change conditions of proliferation rate,carbon sequestration,and microorganism of anaerobic ammonium oxidizing bacteria. It was found that the influent nitrite nitrogen had a great influence on the anaerobic ammonium oxidation reactor. When the influent NH_4~+-N and NO_2~--N concentrations were about 41. 23 mg/L and 51. 63 mg/L namely,the maximum proliferation rate of anaerobic ammoniac was 1. 7 mL/d,the concentration of TS was 94. 03 g/L,the maximum specific anammox activity( SAA) was 0. 31 g/( g·d),the maximum removal efficiency was 91. 18%,the fixed carbon content was 0. 71 mg/mg( N),the microbial richness was the highest,and the dominant strain Candidatus kuenenia was basically unchanged. When the influent NO_2~--N concentration was increased to 102. 99 mg/L,the dominant strain Candidatus kuenenia was significantly reduced,the anaerobic ammonium oxidation reaction process was inhibited,the activity was decreased,and the growth rate of anaerobic ammonium oxidizing bacteria became slower.
引文
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[17]谭发,王敦球,金樾,等.新型一体化厌氧氨氧化反应器启动研究[J].水处理技术,2016,42(7):110-113,117.
[18] Jetten M S,Cirpus I,Kartal B,et al. 1994—2004:10 years of research on the anaerobic oxidation of ammonium[J]. Biochemical Society Transactions,2005,33(1):119-123.
[19]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
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[21] Christian F,Boethler M,Philipp H,et al. Biological treatment of ammonium-rich wastewater by partical nitritation and subsequent anaerobic ammonium oxidation(anammox)in a pilot plant[J].Journal of Biotechnology,2002,99(3):295-306.
[22] Zhang W J,Wang D G,Jin Y. Effects of inorganic carbon on the nitrous oxide emissions and microbial diversity of an anaerobic ammonia oxidation reactor[J]. Bioresource Technology,2017,250:124-130.
[23] Zhang W J,Liu X N,Wang D G,et al. Effects of bamboo charcoal on fouling and microbial diversity in a flat-sheet ceramic membrane bioreactor[J]. Bioresource Technology,2017,243:1020-1026.
[2] Strous M,Fuerst J A,Kramer E H,et al. Missing lithotroph identified as new planctomycete[J]. Nature,1999,400(6743):446-449.
[3] Castrobarros C M,Jia M,van Loosdrecht M C,et al. Evaluating the potential for dissimilatory nitrate reduction by anammox bacteria for municipal wastewater treatment[J]. Bioresource Technology,2017,233:363-372.
[4] Dalsgaard T,Canfield D E,Petersen J,et al. N2production by the anammox reaction in the anoxic water column of Golfo Dulce,Costa Rica[J]. Nature,2003,422(6932):606-608.
[5] Strous M,Jetten M S M. Anaerobic oxidation of methane and ammonium[J]. Annual Review of Microbiology,2004,58:99-117.
[6] Ali M, Okabe S. Anammox-based technologies for nitrogen removal:advances in process start-up and remaining issues[J].Chemosphere,2015,141:144-153.
[7] Chen H,Hu H Y,Chen Q Q,et al. Successful start-up of the anammox process:influence of the seeding strategy on performance and granule properties[J]. Bioresource Technology,2016,211:594-602.
[8] Zhang Z T,Zheng G,Liu S, et al. Extracellular polymeric substances extraction induced the increased purification performance of percoll density gradient centrifugation for anammox bacteria[J]. Chemical Engineering Journal,2016,287:529-536.
[9] Tang C J,Zheng P,Mahmood Q,et al. Start-up and inhibition analysis of the Anammox process seeded with anaerobic granular sludge[J]. Journal of Industrial Microbiology&Biotechnology,2009,36(8):1093-1100.
[10] Ali M,Oshiki M,Awata T,et al. Physiological characterization of anaerobic ammonium oxidizing bacterium“Candidatus jetteniacaeni”[J]. Environmental Microbiology,2015,17(6):2172-2189.
[11] Strous M,Heijnen J J,Kuenen J G,et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology&Biotechnology,1998,50(5):589-596.
[12] Jetten M S,Strous M,Kt P S,et al. The anaerobic oxidation of ammonium[J]. Fems Microbiology Reviews,1998,22(5):421-437.
[13] Lotti T,Wr V D S,Kleerebezem R,et al. The effect of nitrite inhibition on the anammox process[J]. Water Research,2012,46(8):2559-2569.
[14] Strous M,Kuenen J G,Jetten M S M. Key physiology of anaerobic ammonium oxidation[J]. Applied&Environmental Microbiology,1999,65(7):3248-3250.
[15] Raudkivi M,Zekker I,Rikmann E,et al. Nitrite inhibition and limitation-the effect of nitrite spiking on anammox biofilm,suspended and granular biomass[J]. Water Science&Technology,2017,75(2):313-321.
[16]谭发,王敦球,金樾,等.有机碳源对一体化厌氧氨氧化反应器脱氮性能的影响[J].安全与环境工程,2016,23(4):44-50.
[17]谭发,王敦球,金樾,等.新型一体化厌氧氨氧化反应器启动研究[J].水处理技术,2016,42(7):110-113,117.
[18] Jetten M S,Cirpus I,Kartal B,et al. 1994—2004:10 years of research on the anaerobic oxidation of ammonium[J]. Biochemical Society Transactions,2005,33(1):119-123.
[19]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[20]任南琪,王爱杰.厌氧生物技术原理与应用[M].北京:化学工业出版社,2004.
[21] Christian F,Boethler M,Philipp H,et al. Biological treatment of ammonium-rich wastewater by partical nitritation and subsequent anaerobic ammonium oxidation(anammox)in a pilot plant[J].Journal of Biotechnology,2002,99(3):295-306.
[22] Zhang W J,Wang D G,Jin Y. Effects of inorganic carbon on the nitrous oxide emissions and microbial diversity of an anaerobic ammonia oxidation reactor[J]. Bioresource Technology,2017,250:124-130.
[23] Zhang W J,Liu X N,Wang D G,et al. Effects of bamboo charcoal on fouling and microbial diversity in a flat-sheet ceramic membrane bioreactor[J]. Bioresource Technology,2017,243:1020-1026.