桑黄乙醇提取物的美白功效评价
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摘要
探讨桑黄(Phellinus igniarius)乙醇提取物的美白功效。分别通过DPPH自由基清除实验、蘑菇酪氨酸酶抑制实验、B16F10黑色素瘤细胞胞内酪氨酸酶活性实验和B16F10黑色素瘤细胞黑色素含量测定实验评价桑黄乙醇提取物的美白活性。结果表明:桑黄乙醇提取物从1 mg/mL的浓度开始,对B16F10黑色素瘤细胞具有了极显著的细胞毒性,抑制细胞生长;其浓度为0.25、0.5 mg/mL时,对DPPH自由基的清除率分别达到了71.22%和80.94%,具有与维生素C接近的抗氧化活性;另外,其在浓度为0.25、0.5 mg/mL时,对蘑菇酪氨酸酶的抑制率分别达到了48.33%和65.12%,并且可显著降低B16F10黑色素瘤细胞胞内酪氨酸酶活性(P<0.01)和黑色素含量(P<0.01)。研究结果说明,桑黄乙醇提取物具有较强的美白功效。
The whitening activity of a Phellinus igniarius ethanolic extract was studied. The whitening effect was assessed by the DPPH free radical scavenging assay, the mushroom tyrosinase actvitity assay and the tests of cell viability, intracellular tyrosinase activity and melanin content in B16 F10 cells. The results showed that Phellinus igniarius ethanolic extract had severe cytotoxicity and inhibited the growth of B16 F10 cells at a concentration of 1 mg/mL. At the concentrations of 0. 25 and 0. 5 mg/mL, the DPPH clearance rates reached 71.22% and 80.94% respectively, which were comparable to that of vitamin C. Moreover, at the concentrations of 0.25 and 0.5 mg/mL, the mushroom tyrosinase inhibition rates were 65.12% and 48.33%,and the intracellular tyrosinase activity and melanin content in B16 F10 cells were significantly reduced(P<0.01). These findings suggested that the ethanolic extract of Phellinus igniarius had a strong whitening effect.
The whitening activity of a Phellinus igniarius ethanolic extract was studied. The whitening effect was assessed by the DPPH free radical scavenging assay, the mushroom tyrosinase actvitity assay and the tests of cell viability, intracellular tyrosinase activity and melanin content in B16 F10 cells. The results showed that Phellinus igniarius ethanolic extract had severe cytotoxicity and inhibited the growth of B16 F10 cells at a concentration of 1 mg/mL. At the concentrations of 0. 25 and 0. 5 mg/mL, the DPPH clearance rates reached 71.22% and 80.94% respectively, which were comparable to that of vitamin C. Moreover, at the concentrations of 0.25 and 0.5 mg/mL, the mushroom tyrosinase inhibition rates were 65.12% and 48.33%,and the intracellular tyrosinase activity and melanin content in B16 F10 cells were significantly reduced(P<0.01). These findings suggested that the ethanolic extract of Phellinus igniarius had a strong whitening effect.
引文
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[14]王建明,燕瑾,孙红,等.4种野生菌子实体乙醇提取物体外抗氧化活性的研究[J].食品安全质量检测学报,2016,7(2):676-681.
[15]刘晓英,严安,刘光荣,等.松茸提取物在护肤品中的美白效果[J].日用化学品科学,2008,31(10):33-35.
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[17]郭松鹤.高合意,曾飒.等.美白化妆品细胞水平功效评价方法的研究进展[J].香料香精化妆品,2016(5):59-62,68.
[18]薛春雨,李蠡,邢新.α-黑素细胞刺激素对皮肤黑素合成的调控作用[J].医学研究生学报,2004,17(9):823-828.
[19] BRENNER M, HEARING VJ. The protective role of melanin against UV damage in human skin[J].Photochemistry and Photobiology,2008,84(3):539-549.
[20] NARAYANAN DL, SALADI RN, Fox JL. Review:Ultraviolet radiation and skin cancer[J]. International Journal of Dermatology,2010,49(9):978-986.
[21]黄立森,庞海月,吴黉坦,等.4种中草药乙醇提取物体外抗氧化及美白活性研究[J].日用化学工业,2016,46(6):349-352.
[2]张小青,戴玉成.中国真菌志第二十九卷锈革孔菌科[M].北京:科学出版社,2005:117-119.
[3]回晶,李其久,边媛媛,等.桑黄总黄酮超声提取工艺及其生物活性研究[J].食品科学,2010,31(24):195-198.
[4] AJITH TA, JANARDHANAN KK. Antioxidant and antihepatotoxic activities of Phellinus rimosus(Berk)Pilat[J]. Journal of Ethnopharmacology,2002,81(3):387-391.
[5]沈雪梅,王荣庆,王雪梅,等.桑黄提取物体外美容功效研究[J].日用化学工业,2016,46(9):519-523.
[6]任顺成,王鹏,王国良,等.常见食用豆类中黄酮类化合物含量的测定[J].中国粮油学报,2009,24(7):132-137.
[7] KIM A, YIM NH, IM M, et al. Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells[J]. BMC Complementary and Alternative Medicine,2013,13:214-223.
[8]余昊阳,熊智,郭一荣,等.牡丹花蕾提取物体外抗氧化、美白活性研究[J].日用化学品科学,2017,40(6):24-29.
[9] BAE JS, HAN M, YAO C, et al. Chaetocin inhibits IBMX-induced melanogenesis in B16F10 mouse melanoma cells through activation of ERK[J].Chemico-Biological Interactions,2016,245:66-71.
[10] NASR BOUZAIENE N, CHAABANE F, SASSI A, et al. Effect of apigenin-7-glucoside, genkwanin and naringenin on tyrosinase activity and melanin synthesis in B16F10 melanoma cells[J].Life Sciences,2016,144:80-85.
[11]陶丽莉,刘洋,吴金昊,等.化妆品美白功效评价方法研究进展[J].日用化学品科学,2015,38(3):15-21.
[12]潘伟,刘瑞娜,章炉军,等.猴头菌提取物抗氧化活性研究[J].食用菌学报,2012,19(2):95-99.
[13]秦铭铭,耿燕,栾雪,等.药食用真菌提取物抗氧化活性研究[J].食品与生物技术学报,2017,36(1):80-86.
[14]王建明,燕瑾,孙红,等.4种野生菌子实体乙醇提取物体外抗氧化活性的研究[J].食品安全质量检测学报,2016,7(2):676-681.
[15]刘晓英,严安,刘光荣,等.松茸提取物在护肤品中的美白效果[J].日用化学品科学,2008,31(10):33-35.
[16]郭立群,王敏.化妆品功效评价(Ⅶ)——细胞生物学在化妆品功效评价中的应用[J].日用化学工业,2018,48(7):371-377.
[17]郭松鹤.高合意,曾飒.等.美白化妆品细胞水平功效评价方法的研究进展[J].香料香精化妆品,2016(5):59-62,68.
[18]薛春雨,李蠡,邢新.α-黑素细胞刺激素对皮肤黑素合成的调控作用[J].医学研究生学报,2004,17(9):823-828.
[19] BRENNER M, HEARING VJ. The protective role of melanin against UV damage in human skin[J].Photochemistry and Photobiology,2008,84(3):539-549.
[20] NARAYANAN DL, SALADI RN, Fox JL. Review:Ultraviolet radiation and skin cancer[J]. International Journal of Dermatology,2010,49(9):978-986.
[21]黄立森,庞海月,吴黉坦,等.4种中草药乙醇提取物体外抗氧化及美白活性研究[J].日用化学工业,2016,46(6):349-352.