极地雪藻提取物对皮肤UVB损伤的修复作用研究
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摘要
研究了极地雪藻提取物对氧化应激下成纤维细胞(NIH-3T3细胞)的保护作用及UVB辐射造成的皮肤光损伤的修复作用。采用MTT法测试极地雪藻提取物细胞毒性,通过给药后过氧化氢细胞损伤模型的存活率评价其对NIH-3T3细胞的保护作用;利用UVB辐射昆明鼠剃毛区皮肤28天造模,造模成功后涂抹药物,通过H&E染色、胶原纤维染色(Masson染色)与免疫组化染色(IHC染色)检测IL-1、IL-6、TNF-α以评价其对UVB光损伤皮肤的修复作用。结果显示,极地雪藻提取物对NIH-3T3细胞没有明显的细胞毒性,并且能提高氧化应激状态下的NIH-3T3细胞存活率;H&E染色显示,UVB辐射小鼠皮肤导致皮肤表皮厚度增加,皮肤胶原量减少;IHC研究表明炎症因子IL-1、IL-6、TNF-α表达量增加;模型给药后,表皮厚度恢复正常,胶原量增加,IL-1、IL-6、TNF-α表达量降低。极地雪藻提取物对氧化应激下的NIH-3T3细胞有保护作用,并可以修复UVB辐射造成的皮肤损伤;推测极地雪藻的修复作用可能是通过对氧化应激和炎症反应的调节起作用。
The protective effects of Chlamydomonas nivalis extract on NIH-3 T3 cells under the oxidative stress and skin photodamage induced by UVB were evaluated. The cytotoxicity of the extract and the protective effect against hydrogen peroxide on NIH-3 T3 cells were evaluated by MTT method. Chlamydomonas nivalis extract was applied to the dorsal skin of mice after UVB irradiation daily for 28 days to complete the photoaging model.Moreover, the skin was analyzed by H&E staining and Masson staining, then the expression of IL-1, IL-6 and TNF-α was analyzed by immunohistochemistry(IHC). The results show that Chlamydomonas nivalis extract exhibits no obvious cytotoxicity for NIH-3 T3 cells and increases the cell vitality under the oxidative stress. In animal experiment, the results of H&E staining show that UVB irradiation induces epidermis thickening and the collagen decreasing. The extract recovers the epidermis thickness and the collagen is increased. The increase of TNF-α and interleukin including IL-1 and IL-6 were induced by UVB, as shown in IHC results. The extract is shown to decrease the expression quantities of IL-1, IL-6 and TNF-α. Chlamydomonas nivalis extract protects the NIH-3 T3 cells from oxidative stress induced by hydrogen peroxide and repairs the skin damage from UVB irradiation. It is speculated that the alleviative efficacy of Chlamydomonas nivalis extract may work by regulating oxidative stress and inflammation.
The protective effects of Chlamydomonas nivalis extract on NIH-3 T3 cells under the oxidative stress and skin photodamage induced by UVB were evaluated. The cytotoxicity of the extract and the protective effect against hydrogen peroxide on NIH-3 T3 cells were evaluated by MTT method. Chlamydomonas nivalis extract was applied to the dorsal skin of mice after UVB irradiation daily for 28 days to complete the photoaging model.Moreover, the skin was analyzed by H&E staining and Masson staining, then the expression of IL-1, IL-6 and TNF-α was analyzed by immunohistochemistry(IHC). The results show that Chlamydomonas nivalis extract exhibits no obvious cytotoxicity for NIH-3 T3 cells and increases the cell vitality under the oxidative stress. In animal experiment, the results of H&E staining show that UVB irradiation induces epidermis thickening and the collagen decreasing. The extract recovers the epidermis thickness and the collagen is increased. The increase of TNF-α and interleukin including IL-1 and IL-6 were induced by UVB, as shown in IHC results. The extract is shown to decrease the expression quantities of IL-1, IL-6 and TNF-α. Chlamydomonas nivalis extract protects the NIH-3 T3 cells from oxidative stress induced by hydrogen peroxide and repairs the skin damage from UVB irradiation. It is speculated that the alleviative efficacy of Chlamydomonas nivalis extract may work by regulating oxidative stress and inflammation.
引文
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[2]Karran P,Brem R.Protein oxidation,UVA and human DNA repair[J].DNA Repair,2016,44:178-185.
[3]Nishisgori C.Current concept of photocarcinogenesis[J].Photochem Photobiol Science,2015(14):1713-1721.
[4]Saito P,Melo C P B,Martinez R M,et al.The lipid mediator resolvin D1 reduces the skin inflammation and oxidative stress induced by UV Irradiation in hairless mice[J].Frontiers in Pharmacology,2018(9):1242.
[5]Geng Yuhuan,Wei Dong,Li Guoji.Reasearch development and prospect analysis of Chlamydomonas nivails[J].Modern Food Science and Technology,2006,22(3):243-246.
[6]Aquasearch.Astaxanthin as an antioxidant:a summary[R].The Astafactor Technical Report,2000.
[7]Geng Yuhuan,Li lin,Wei Dong,et al.Physiological adaptability of Chlamydomonas nivalis under ultraviolet radiation[J].Food Science,2006,27(3):40-43.
[8]Tanigawa T,Kanazawa S,Ichibori R,et al.(+)-Catechin protects dermal fibroblasts against oxidative stress-induced apoptosis[J].BMC Complementary and Alternative Medicine,2014,14(1):133.
[9]Hoham R W.Microbial ecology of snow and freshwater ice with emphasis on snow algae[M].New York:Cambridge University Press,2001:166-228.
[10]Kim B K,Joo H M,Lee B,et al.Physiological characteristics and related biochemical parameters of snow algae from King George Island,Antarctica[J].Ocean Science Journal,2018:1-10.
[11]Kuang J,Xie M,Wei X.The NALP3 inflammasome is required for collagen synthesis via the NF-κB pathway[J].International Journal of Molecular Medicine,2018,41(4):2279-2287.
[12]Xu P,Zhang M,Wang X,et al.Antioxidative effect of quetiapine on acute ultraviolet-B-induced skin and HaCaT cell damage[J].International Journal of Molecular Sciences,2018,19(4):953.
[13]Xu Yang,Zhou Bingrong,Wu Di,et al.Effect of baicalin on UVB-mediated photo damages in mice skin[J].Chinese Journal of Aesthetic Medicine,2012,21(1):84-86.
[14]Kammeyer A,Luiten R M.Oxidation events and skin aging[J].Ageing Research Reviews,2015(21):16-29.
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