响应面法优化蛹虫草子实体多糖的提取工艺研究
|
摘要
对蛹虫草子实体多糖热水浸提工艺进行研究。以人工培养的蛹虫草子实体为原料,采用苯酚-硫酸法测定提取液中多糖的提取率为指标,考察提取温度、提取时间、料液比对多糖提取率的影响,分别进行单因素试验和响应面试验优化。结果显示,在单因素试验筛选的水平区间,响应面优化的最佳提取工艺为:在78℃下,按照33 m L/g料液比,提取两次,每次提取1.2 h,其多糖的提取率可达到6.11%,与模型的预测值接近。该工艺简单可靠,为虫草多糖的进一步产业化提供一定指导作用。
The extraction process of polysaccharide was studied in the paper by hot water assisted extraction from Cordyceps militaris. The content of polysaccharides was determined by phenol- vitriol method. The effects of extraction temperature,extraction time,solid- liquid ratio and the number of extraction were investigated on the basis of single factor test. Through response surface methodology,the optimum extraction conditions of polysaccharide were as follows:solid- liquid ratio of 33 m L / g,extraction time of 1. 2 h,extraction temperature of 80 ℃,two time extraction under this process condition,the extraction rate of polysaccharides was 6. 11%. The optimized extraction method was simple and guided large- scale extraction of polysaccharides from Cordyceps militaris.
The extraction process of polysaccharide was studied in the paper by hot water assisted extraction from Cordyceps militaris. The content of polysaccharides was determined by phenol- vitriol method. The effects of extraction temperature,extraction time,solid- liquid ratio and the number of extraction were investigated on the basis of single factor test. Through response surface methodology,the optimum extraction conditions of polysaccharide were as follows:solid- liquid ratio of 33 m L / g,extraction time of 1. 2 h,extraction temperature of 80 ℃,two time extraction under this process condition,the extraction rate of polysaccharides was 6. 11%. The optimized extraction method was simple and guided large- scale extraction of polysaccharides from Cordyceps militaris.
引文
[1]邵力平.真菌分类学[M].北京:中国林业出版社,1984:109.
[2]国家药典委员会.中华人民共和国药典[M].一部.北京:化学工业出版社,2005:75-76.
[3]刘玉军,徐芳,陈波,等.毛细管区带电泳法测定冬虫夏草及人工北冬虫夏草子实体中核苷及碱基成分的含量[J].药物分析杂志,2010,30(1):24-29.
[4]Li S P,Yang F Q,Tsim K W K.Quality control of Cordyceps sinensis,a valued traditional Chinese medicine[J].Journal of pharmaceutical and biomedical analysis,2006,41(5):1571-1584.
[5]Wang M,Meng X Y,Yang R L,et al.Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice[J].Carbohydrate polymers,2012,89(2):461-466.
[6]Wu F,Yan H,Ma X,et al.Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media[J].World Journal of Microbiology and Biotechnology,2012,28:2029-2038.
[7]ZHANG Guo-qing,HUANG Yue-dong,BIAN Yong,et al.Hypoglycemic activity of the fungi Cordyceps militaris,Cordyceps sinensis,Tricholoma mongolicum and Omphalia lapidescens in streptozotocin-induced diabetic rats[J].Applied Microbiology and Biotechnology,2006,72(6):1152-1156.
[8]YANG Jin-yu,ZHANG Wei-yun,SHI Pei-hua,et al.Effects of exopolysaccharide fraction(EPSF)from a cultivated Cordyceps sinensis fungus on c-Myc,c-Fos,and VEGF expression in B16 melanomabearing mice[J].Pathology Research and Practice,2005,201(11):745-750.
[9]Hu Z,Xia F B,Wu X G,et al.Cordyceps sinensis new efficacy composition[J].Edible Fungi of China,2004,23(5):37-38.
[10]鲁晓岩.硫酸-苯酚法测定北冬虫夏草多糖含量[J].食品工业科技,2002,23(4):69-70.
[2]国家药典委员会.中华人民共和国药典[M].一部.北京:化学工业出版社,2005:75-76.
[3]刘玉军,徐芳,陈波,等.毛细管区带电泳法测定冬虫夏草及人工北冬虫夏草子实体中核苷及碱基成分的含量[J].药物分析杂志,2010,30(1):24-29.
[4]Li S P,Yang F Q,Tsim K W K.Quality control of Cordyceps sinensis,a valued traditional Chinese medicine[J].Journal of pharmaceutical and biomedical analysis,2006,41(5):1571-1584.
[5]Wang M,Meng X Y,Yang R L,et al.Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice[J].Carbohydrate polymers,2012,89(2):461-466.
[6]Wu F,Yan H,Ma X,et al.Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media[J].World Journal of Microbiology and Biotechnology,2012,28:2029-2038.
[7]ZHANG Guo-qing,HUANG Yue-dong,BIAN Yong,et al.Hypoglycemic activity of the fungi Cordyceps militaris,Cordyceps sinensis,Tricholoma mongolicum and Omphalia lapidescens in streptozotocin-induced diabetic rats[J].Applied Microbiology and Biotechnology,2006,72(6):1152-1156.
[8]YANG Jin-yu,ZHANG Wei-yun,SHI Pei-hua,et al.Effects of exopolysaccharide fraction(EPSF)from a cultivated Cordyceps sinensis fungus on c-Myc,c-Fos,and VEGF expression in B16 melanomabearing mice[J].Pathology Research and Practice,2005,201(11):745-750.
[9]Hu Z,Xia F B,Wu X G,et al.Cordyceps sinensis new efficacy composition[J].Edible Fungi of China,2004,23(5):37-38.
[10]鲁晓岩.硫酸-苯酚法测定北冬虫夏草多糖含量[J].食品工业科技,2002,23(4):69-70.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |