不同香稻品种苗期耐高温的形态生理响应
|
摘要
【目的】本研究旨在分析不同香稻品种苗期耐高温的形态生理响应特征,筛选出耐热性较好的香稻品种。【方法】在盆栽条件下,于光照培养箱内,以超级常规香稻玉香油占为对照,研究了常规香稻品种巴斯马蒂、桂香占、农香18、湘晚籼13、象牙香占、中香1号、美香占2号在苗期高温处理下的形态生理响应特征。于高温处理前后测定了8个香稻品种苗期各项形态生理指标。【结果】与玉香油占相比,高温胁迫下,巴斯马蒂的株高、单株鲜质量、单株干质量增幅更大,成活率和过氧化物酶活性更高,丙二醛含量反而下降了33.39%,表明巴斯马蒂的耐高温胁迫能力较好。湘晚籼13在高温处理下的单株干质量增幅与玉香油占相近,株成活率较玉香油占低,胞间二氧化碳浓度变幅较小,SOD活性和丙二醛含量的变幅在8个品种中均为最低,说明其耐高温胁迫的能力略低于玉香油占。农香18、象牙香占和美香占2号的成活率分别为13.33%、12.67%和16.00%。桂香占(4.33%)、中香1号(1.33%)在高温处理后的成活率最低,表明两者的耐高温能力均较差。【结论】8个品种耐热性从强到弱依次为巴斯马蒂、玉香油占、湘晚籼13、农香18、象牙香占、美香占2号、桂香占和中香1号。
【Objective】The purpose of this research is to study the morphophysiological responses of different scented rice varieties to high temperature at seedling stage and to screen out heat-resistant varieties.【Method】With the super inbred fragrant rice Yuxiangyouzhan as control, a pot experiment was conducted in a light incubator, to reveal morphophysiological responses of seven inbred fragrant rice varieties(Basmati, Guixiangzhan, Nongxiang 18, Xiangwanxian 13, Xiangyaxiangzhan, Zhongxiang 1, Meixiangzhan 2) to high temperature stress. The morphophysiological indexes of the eight scented rice varieties were determined before and after high temperature treatment.【Result】Compared with Yuxiangyouzhan, the plant height, fresh weight, dry weight increased with a langer rate, the survival rate and POD activity in Basmati were higher under high temperature treatment. The malondialdehyde content was decreased by 33.39% for Basmati under high temperature treatment. It indicated that Basmati had higher tolerance to high temperature stress. The dry weight growth rate per plant of Xiangwanxian 13 under high temperature stress was equal to that of Yuxiangyouzhan with lower survival rate than Yuxiangyouzhan, and the change rate of intercellular CO2 concentration was lower after high temperature treatment, the SOD activity and the change rate of malondialdehyde content was the lowest among the eight varieties, indicating that its ability to withstand high temperature stress was slightly lower than that of Yuxiangyouzhan. The survival rates of Nongxiang18, Xiangyaxiangzhan and Meixiangzhan 2 were 13.33%, 12.67% and 16.00%, respectively. The survival rates of Guixiangzhan(4.33%) and Zhongxiang 1(1.33%) under high temperature stress were the lowest among the eight varieties, indicating their weak high temperature tolerance.【Conclusion】In general, the heat resistance of the eight varieties from strong to weak was Basmati>Yuxiangyouzhan>Xiangwanxian 13>Nongxiang 18>Xiangyaxiangzhan>Meixiangzhan>Guixiangzhan>Zhongxiang 1.
【Objective】The purpose of this research is to study the morphophysiological responses of different scented rice varieties to high temperature at seedling stage and to screen out heat-resistant varieties.【Method】With the super inbred fragrant rice Yuxiangyouzhan as control, a pot experiment was conducted in a light incubator, to reveal morphophysiological responses of seven inbred fragrant rice varieties(Basmati, Guixiangzhan, Nongxiang 18, Xiangwanxian 13, Xiangyaxiangzhan, Zhongxiang 1, Meixiangzhan 2) to high temperature stress. The morphophysiological indexes of the eight scented rice varieties were determined before and after high temperature treatment.【Result】Compared with Yuxiangyouzhan, the plant height, fresh weight, dry weight increased with a langer rate, the survival rate and POD activity in Basmati were higher under high temperature treatment. The malondialdehyde content was decreased by 33.39% for Basmati under high temperature treatment. It indicated that Basmati had higher tolerance to high temperature stress. The dry weight growth rate per plant of Xiangwanxian 13 under high temperature stress was equal to that of Yuxiangyouzhan with lower survival rate than Yuxiangyouzhan, and the change rate of intercellular CO2 concentration was lower after high temperature treatment, the SOD activity and the change rate of malondialdehyde content was the lowest among the eight varieties, indicating that its ability to withstand high temperature stress was slightly lower than that of Yuxiangyouzhan. The survival rates of Nongxiang18, Xiangyaxiangzhan and Meixiangzhan 2 were 13.33%, 12.67% and 16.00%, respectively. The survival rates of Guixiangzhan(4.33%) and Zhongxiang 1(1.33%) under high temperature stress were the lowest among the eight varieties, indicating their weak high temperature tolerance.【Conclusion】In general, the heat resistance of the eight varieties from strong to weak was Basmati>Yuxiangyouzhan>Xiangwanxian 13>Nongxiang 18>Xiangyaxiangzhan>Meixiangzhan>Guixiangzhan>Zhongxiang 1.
引文
[1]罗昊文,钟卓君,聂俊,唐湘如.超声波处理对粤晶丝苗生理特性及产量、品质的影响.中国稻米,2017,23(2):64-67.Luo H W,Zhong Z J,Nie J,Tang X R.Effects of ultrasound on physiological characters,yield and quality of rice Yuejingsimiao.China Rice,2017,23(2):64-67(in Chinese)
[2]张洪程,龚金龙.中国水稻种植机械化高产农艺研究现状及发展探讨.中国农业科学,2014,47(07):1273-1289.Zhang H C,Gong J L.Research status and development discussion on high-yielding agronomy of mechanized planting rice in China.Sci Agric Sin,2014,47(7):1273-1289.(in Chinese with English abstract)
[3]贾士荣,袁潜华,王丰,姚克敏,裴新梧,胡凝,王志兴,王旭静,柳武革,钱前.转基因水稻基因飘流研究十年回顾.中国农业科学,2014,47(1):1-10.Jia S R,Yuan Q H,Wang F,Yao K M,Pei X W,Hu N,Wang Z X,Wang X J,Liu W G,Qian Q.What we have learnt in ten years’study of rice transgene flow.Sci Agric Sin,2014,47(1):1-10.(in Chinese with English abstract)
[4]沈永平,王国亚.IPCC第一工作组第五次评估报告对全球气候变化认知的最新科学要点.冰川冻土,2013,35(5):1068-1076.Shen Y P,Wang G Y.Key findings and assessment results of IPCC WGI Fifth assessment report.J Glaciol Geocryol,2013,35(5):1068-1076.(in Chinese)
[5]张桂莲,陈立云,雷东阳,张顺堂.水稻耐热性研究进展.杂交水稻,2005,20(1):1-5.Zhang G L,Chen L Y,Lei D Y,Zhang S T.Progresses in research on heat tolerance in rice.Hybrid Rice,2005,20(1):1-5.(in Chinese with English abstract)
[6]黄显波,严寒,胡建林,田志宏.高温胁迫下水稻幼苗几个相关生理指标的变化.长江大学学报:自然科学版农学卷,2008,5(2):50-53.Huang X B,Yan H,Hu J L,Tian Z H.Changes of several related physiological indexes of rice seedlings under high temperature stress.J Yangtze Univ:Nat Sci Edit,Agric Sci,2008,5(2):50-53.(in Chinese with English abstract)
[7]李仁忠,刘海英,李建业,叶海龙,余久红,金志凤.浙江省早稻高温热害发生规律及防御措施.浙江气象,2011,32(1):23-26,45.Li R Z,Liu H Y,Li J Y,Ye H L,Yu J H,Jin Z F.Occurrence law and prevention measures of high temperature heat damage of early rice in Zhejiang Province.Zhejiang Meteorol,2011,32(1):23-26,45.(in Chinese)
[8]熊玉江,乔伟,姚付启,罗文兵,袁念念,付浩龙.气候变化和灌溉方式对稻田需水量的影响.长江科学院院报,2018,35(4):76-80.Xiong Y J,Qiao W,Yao F Q,Luo W B,Yuan N N,Fu HL.Effect of climate change and irrigation pattern on water demand of paddy field.J Yangtze River Sci Res Instit,2018,35(4):76-80.(in Chinese with English abstract)
[9]赵庆雷,吴修,王瑜,李曰鹏,陈博聪,王佳,马加清.扬花期高温条件下施肥量对水稻高位分蘖及产量性状的影响.山东农业科学,2016,48(8):61-66.Zhao Q L,Wu X,Wang Y,Li Y P,Chen B C,Wang J,Ma J Q.Effects of high temperature at flowering stage and fertilization on high-node tillering and yield traits of rice.Shandong Agric Sci.2016,48(8):61-66.(in Chinese)
[10]陈蔚.高温对水稻生育特性的影响及其防御对策.湖北农业科学,2010,49(8):1817-1818.Chen W.Effects of high temperature on growth and development characteristics of rice and its preventive measures.Hubei Agric Sci,2010,49(8):1817-1818.(in Chinese with English abstract)
[11]段骅,杨建昌.高温对水稻的影响及其机制的研究进展.中国水稻科学,2012,26(4):393-400.Duan H,Yang J C.Research Advances in the effect of high temperature on rice and its mechanism.Chin J Rice Sci.2012,26(4):393-400.(in Chinese with English abstract)
[12]王小菁,萧浪涛,董爱武,王台,钱前,漆小泉,陈凡,左建儒,杨淑华,顾红雅,陈之端,姜里文,白永飞,孔宏智,种康.2016年中国植物科学若干领域重要研究进展.植物学报,2017,52(4):394-452.Wang X J,Xiao L T,Dong A W,Wang T,Qian Q,Qi XQ,Chen F,Zuo J R,Yang S H,Gu H Y,Chen Z D,Jiang L W,Bai Y F,Kong H Z,Zhong K.Research progress in several fields of Chinese plant science in 2016.Chin Bull Bot,2017,52(4):394-452.(in Chinese)
[13]Zhang X J,Zhang S Y,Li C Q,Zhang W H.Effects of high temperature stress on growth of stress-tolerant rice seedlings with resistibility.Agric Sci&Technol,2014,15(4):576-578,584.
[14]朱雪梅,柯永培,邵继荣,林立金,杨远祥.高温胁迫对重穗型水稻品种叶片活性氧代谢的影响.种子,2005,24(3):25-27,32.Zhu X M,Ke Y P,Shao J R,Lin L J,Yang Y X.Effects of high temperature stress on activated oxygen metabolism of the heavy panicle type of medium indica hybrid rice.Seed,2005,24(3):25-27,32.(in Chinese with English abstract)
[15]李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000.Li H S.Principles and Techniques of Plant Physiology and Biochemistry Experiments.Beijing:Higher Education Press,2000.(in Chinese)
[16]Wahid A,Gelani S,Ashraf M,Foolad M R.Heat tolerance in plants:An overview.Environ Exp Bot,2007,61(3):199-223.
[17]杨纯明,Heilman L J.谢国禄译.短期高温对水稻生长发育和产量的影响.国外作物育种,1994,13(2):4-5.Yang C M,Heilman L J.Xie G L Trans.Effects of short-term high temperature on rice growth and yield.Forei Crop Breeding,1994,13(2):4-5.(in Chinese)
[18]张桂莲,陈立云,张顺堂,刘国华,唐文邦,贺治洲,王明.抽穗开花期高温对水稻剑叶理化特性的影响.中国农业科学,2007,40(7):1345-1352.Zhang G L,Chen L Y,Zhang S T,Liu G H,Tang W B,He Z Z,Wang M.Effects of high temperature on physiological and biochemical characteristics in flag leaf of rice during heading and flowering period.Sci Agric Sin,2007,40(7):1345-1352.(in Chinese with English abstract)
[19]Barnabás B,J?ger K,Fehér A.The effect of drought and heat stress on reproductive processes in cereals.Plant Cell&Environ,2008,31(1):11-38.
[20]杨卫丽,黄福灯,曹珍珍,雷炳婷,胡东维,程方民.高温胁迫对水稻光合PSⅡ系统伤害及其与叶绿体D1蛋白间关系.作物学报,2013,39(6):1060-1068.Yang W L,Huang F D,Cao Z Z,Lei B T,Hu D W,Cheng F M.Effects of high temperature stress on PSⅡfunction and its relation to D1 protein in chloroplast thylakoid in rice flag leaves.Acta Agron Sin,2013,39(6):1060-1068.(in Chinese with English abstract)
[21]曹云英,赵华.高温胁迫下油菜素内酯对水稻幼苗的保护作用.中国水稻科学,2007,21(5):525-529.Cao Y Y,Zhao H.Protective roles of brassinolide in rice seedlings under heat stress.Chin J Rice Sci,2007,21(5):525-529.(in Chinese with English abstract)
[22]郑小林,董任瑞.水稻热激反应的研究:Ⅲ.高温对水稻幼苗叶片过氧化物酶的影响.湖南农业大学学报,1998,24(6):13-16.Zheng X L,Dong R R.A Study on the response of rice to heat shock:Ⅲ.High temperature’s effect on peroxidase in seedling leaves of rice.J Hunan Agric Unive,1998,24(06):13-16.(in Chinese with English abstract)
[23]陈贵,胡文玉,谢甫绨,张立军.提取植物体内MDA的溶剂及MDA作为衰老指标的探讨.植物生理学通讯,1991,27(1):44-46.Chen G,Hu W Y,Xie P T,Zhang L J.Solvent for extracting malondialdehyde in plant as an index of senescence.Plant Physiol Commun,1997,27(1):44-46.(in Chinese)
[24]陈少裕.膜脂过氧化对植物细胞的伤害.植物生理学通讯,1991,27(2):84-90.Chen S Y.Membrane-lipid peroxidation and plant stress.Chin Bull Bot,1991,27(2):84-90.(in Chinese with English abstract)
[2]张洪程,龚金龙.中国水稻种植机械化高产农艺研究现状及发展探讨.中国农业科学,2014,47(07):1273-1289.Zhang H C,Gong J L.Research status and development discussion on high-yielding agronomy of mechanized planting rice in China.Sci Agric Sin,2014,47(7):1273-1289.(in Chinese with English abstract)
[3]贾士荣,袁潜华,王丰,姚克敏,裴新梧,胡凝,王志兴,王旭静,柳武革,钱前.转基因水稻基因飘流研究十年回顾.中国农业科学,2014,47(1):1-10.Jia S R,Yuan Q H,Wang F,Yao K M,Pei X W,Hu N,Wang Z X,Wang X J,Liu W G,Qian Q.What we have learnt in ten years’study of rice transgene flow.Sci Agric Sin,2014,47(1):1-10.(in Chinese with English abstract)
[4]沈永平,王国亚.IPCC第一工作组第五次评估报告对全球气候变化认知的最新科学要点.冰川冻土,2013,35(5):1068-1076.Shen Y P,Wang G Y.Key findings and assessment results of IPCC WGI Fifth assessment report.J Glaciol Geocryol,2013,35(5):1068-1076.(in Chinese)
[5]张桂莲,陈立云,雷东阳,张顺堂.水稻耐热性研究进展.杂交水稻,2005,20(1):1-5.Zhang G L,Chen L Y,Lei D Y,Zhang S T.Progresses in research on heat tolerance in rice.Hybrid Rice,2005,20(1):1-5.(in Chinese with English abstract)
[6]黄显波,严寒,胡建林,田志宏.高温胁迫下水稻幼苗几个相关生理指标的变化.长江大学学报:自然科学版农学卷,2008,5(2):50-53.Huang X B,Yan H,Hu J L,Tian Z H.Changes of several related physiological indexes of rice seedlings under high temperature stress.J Yangtze Univ:Nat Sci Edit,Agric Sci,2008,5(2):50-53.(in Chinese with English abstract)
[7]李仁忠,刘海英,李建业,叶海龙,余久红,金志凤.浙江省早稻高温热害发生规律及防御措施.浙江气象,2011,32(1):23-26,45.Li R Z,Liu H Y,Li J Y,Ye H L,Yu J H,Jin Z F.Occurrence law and prevention measures of high temperature heat damage of early rice in Zhejiang Province.Zhejiang Meteorol,2011,32(1):23-26,45.(in Chinese)
[8]熊玉江,乔伟,姚付启,罗文兵,袁念念,付浩龙.气候变化和灌溉方式对稻田需水量的影响.长江科学院院报,2018,35(4):76-80.Xiong Y J,Qiao W,Yao F Q,Luo W B,Yuan N N,Fu HL.Effect of climate change and irrigation pattern on water demand of paddy field.J Yangtze River Sci Res Instit,2018,35(4):76-80.(in Chinese with English abstract)
[9]赵庆雷,吴修,王瑜,李曰鹏,陈博聪,王佳,马加清.扬花期高温条件下施肥量对水稻高位分蘖及产量性状的影响.山东农业科学,2016,48(8):61-66.Zhao Q L,Wu X,Wang Y,Li Y P,Chen B C,Wang J,Ma J Q.Effects of high temperature at flowering stage and fertilization on high-node tillering and yield traits of rice.Shandong Agric Sci.2016,48(8):61-66.(in Chinese)
[10]陈蔚.高温对水稻生育特性的影响及其防御对策.湖北农业科学,2010,49(8):1817-1818.Chen W.Effects of high temperature on growth and development characteristics of rice and its preventive measures.Hubei Agric Sci,2010,49(8):1817-1818.(in Chinese with English abstract)
[11]段骅,杨建昌.高温对水稻的影响及其机制的研究进展.中国水稻科学,2012,26(4):393-400.Duan H,Yang J C.Research Advances in the effect of high temperature on rice and its mechanism.Chin J Rice Sci.2012,26(4):393-400.(in Chinese with English abstract)
[12]王小菁,萧浪涛,董爱武,王台,钱前,漆小泉,陈凡,左建儒,杨淑华,顾红雅,陈之端,姜里文,白永飞,孔宏智,种康.2016年中国植物科学若干领域重要研究进展.植物学报,2017,52(4):394-452.Wang X J,Xiao L T,Dong A W,Wang T,Qian Q,Qi XQ,Chen F,Zuo J R,Yang S H,Gu H Y,Chen Z D,Jiang L W,Bai Y F,Kong H Z,Zhong K.Research progress in several fields of Chinese plant science in 2016.Chin Bull Bot,2017,52(4):394-452.(in Chinese)
[13]Zhang X J,Zhang S Y,Li C Q,Zhang W H.Effects of high temperature stress on growth of stress-tolerant rice seedlings with resistibility.Agric Sci&Technol,2014,15(4):576-578,584.
[14]朱雪梅,柯永培,邵继荣,林立金,杨远祥.高温胁迫对重穗型水稻品种叶片活性氧代谢的影响.种子,2005,24(3):25-27,32.Zhu X M,Ke Y P,Shao J R,Lin L J,Yang Y X.Effects of high temperature stress on activated oxygen metabolism of the heavy panicle type of medium indica hybrid rice.Seed,2005,24(3):25-27,32.(in Chinese with English abstract)
[15]李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000.Li H S.Principles and Techniques of Plant Physiology and Biochemistry Experiments.Beijing:Higher Education Press,2000.(in Chinese)
[16]Wahid A,Gelani S,Ashraf M,Foolad M R.Heat tolerance in plants:An overview.Environ Exp Bot,2007,61(3):199-223.
[17]杨纯明,Heilman L J.谢国禄译.短期高温对水稻生长发育和产量的影响.国外作物育种,1994,13(2):4-5.Yang C M,Heilman L J.Xie G L Trans.Effects of short-term high temperature on rice growth and yield.Forei Crop Breeding,1994,13(2):4-5.(in Chinese)
[18]张桂莲,陈立云,张顺堂,刘国华,唐文邦,贺治洲,王明.抽穗开花期高温对水稻剑叶理化特性的影响.中国农业科学,2007,40(7):1345-1352.Zhang G L,Chen L Y,Zhang S T,Liu G H,Tang W B,He Z Z,Wang M.Effects of high temperature on physiological and biochemical characteristics in flag leaf of rice during heading and flowering period.Sci Agric Sin,2007,40(7):1345-1352.(in Chinese with English abstract)
[19]Barnabás B,J?ger K,Fehér A.The effect of drought and heat stress on reproductive processes in cereals.Plant Cell&Environ,2008,31(1):11-38.
[20]杨卫丽,黄福灯,曹珍珍,雷炳婷,胡东维,程方民.高温胁迫对水稻光合PSⅡ系统伤害及其与叶绿体D1蛋白间关系.作物学报,2013,39(6):1060-1068.Yang W L,Huang F D,Cao Z Z,Lei B T,Hu D W,Cheng F M.Effects of high temperature stress on PSⅡfunction and its relation to D1 protein in chloroplast thylakoid in rice flag leaves.Acta Agron Sin,2013,39(6):1060-1068.(in Chinese with English abstract)
[21]曹云英,赵华.高温胁迫下油菜素内酯对水稻幼苗的保护作用.中国水稻科学,2007,21(5):525-529.Cao Y Y,Zhao H.Protective roles of brassinolide in rice seedlings under heat stress.Chin J Rice Sci,2007,21(5):525-529.(in Chinese with English abstract)
[22]郑小林,董任瑞.水稻热激反应的研究:Ⅲ.高温对水稻幼苗叶片过氧化物酶的影响.湖南农业大学学报,1998,24(6):13-16.Zheng X L,Dong R R.A Study on the response of rice to heat shock:Ⅲ.High temperature’s effect on peroxidase in seedling leaves of rice.J Hunan Agric Unive,1998,24(06):13-16.(in Chinese with English abstract)
[23]陈贵,胡文玉,谢甫绨,张立军.提取植物体内MDA的溶剂及MDA作为衰老指标的探讨.植物生理学通讯,1991,27(1):44-46.Chen G,Hu W Y,Xie P T,Zhang L J.Solvent for extracting malondialdehyde in plant as an index of senescence.Plant Physiol Commun,1997,27(1):44-46.(in Chinese)
[24]陈少裕.膜脂过氧化对植物细胞的伤害.植物生理学通讯,1991,27(2):84-90.Chen S Y.Membrane-lipid peroxidation and plant stress.Chin Bull Bot,1991,27(2):84-90.(in Chinese with English abstract)