Box-Behnken响应面法优选凤丹皮中苷类成分的醇提工艺
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摘要
目的优选凤丹皮中苷类成分的醇提工艺。方法在单因素试验的基础上,以乙醇浓度、料液比和提取时间3个因素为自变量,以氧化芍药苷、芍药苷、苯甲酰芍药苷和浸膏得率的综合评分为因变量,采用基于Box-Behnken设计的响应面法优化凤丹皮中苷类成分的醇提工艺。结果最优醇提工艺为60%乙醇,料液比1:70,提取2次,每次90min。结论优选的醇提工艺方法简单、稳定可行,可为进一步的生产开发和应用研究提供参考。
Objective To optimize the ethanol extraction process of glycosides from cortex paeoniae sinensis. Methods On the basis of single factor experiment, taking ethanol concentration, solid-liquid ratio and extraction time as independent variables, and taking the comprehensive scores of paeoniflorin oxide, paeoniflorin, benzoyl paeoniflorin and extract yield as dependent variables, response surface methodology based on Box-Behnken design was used to optimize the ethanol extraction process of glycosides from cortex paeoniae sinensis. Results The optimum ethanol extraction process was 60% ethanol, the ratio of material to liquid was 1:70, extraction twice, 90 minutes each time. Conclusion The optimized ethanol extraction process is simple, stable and feasible, which can provide reference for further production development and application research.
Objective To optimize the ethanol extraction process of glycosides from cortex paeoniae sinensis. Methods On the basis of single factor experiment, taking ethanol concentration, solid-liquid ratio and extraction time as independent variables, and taking the comprehensive scores of paeoniflorin oxide, paeoniflorin, benzoyl paeoniflorin and extract yield as dependent variables, response surface methodology based on Box-Behnken design was used to optimize the ethanol extraction process of glycosides from cortex paeoniae sinensis. Results The optimum ethanol extraction process was 60% ethanol, the ratio of material to liquid was 1:70, extraction twice, 90 minutes each time. Conclusion The optimized ethanol extraction process is simple, stable and feasible, which can provide reference for further production development and application research.
引文
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[3]张健萍,李连珍,赵红江,等.牡丹皮的化学成分、药理作用及临床应用研究概况[J].中华中医药杂志,2006,21(5):295～297.
[4]李湘玲.丹皮的综合提取工艺探讨[J].现代中药研究与实践,2005,19(4):58～59.
[5]王培,赵宏苏,龙全江,等.多指标正交试验优选凤丹皮醇提工艺[J].现代中药研究与实践,2018,32(1):53～56.
[6]范梦雪,秦昆明,丁斐,等.Box-Behnken响应面法结合多指标综合加权法优选升麻中酚酸类成分的提取工艺[J].中国药房,2016,27(13):1835～1838.
[7]王永菲,王成国.响应面法的理论与应用[J].中央民族大学学报(自然科学版),2005,14(3):236～240.
[8]周启超,姚欢欢,陈凤丽,等.响应面法优选牡丹果荚中丹皮酚和芍药苷提取工艺的研究[J].植物研究,2014,34(2):271～275.
[9]俞年军,吴德玲,王艳华,等.高效液相色谱法同时测定丹皮总苷部位3种单萜苷类[J].中国医院药学杂志,2013,33(11):769～770.
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[11]王云.牡丹皮有效成分药理及分析方法研究进展[J].亚太传统医药,2016,12(16):63～64.
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[13]王艳华,吴德玲,刘劲松,等.牡丹皮中丹皮总苷的提取工艺研究[J].安徽中医学院学报,2012,31(2):69～71.
[14]邓瑞雪,李小方,刘一琼,等.响应面法优化凤丹籽饼粕中单萜苷的超声提取工艺[J].食品工业科技,2015,36(10):269～274.
[15]王留兴.牡丹皮乙醇提取工艺研究[J].长春中医药大学学报,2008,24(1):35～36.
[16]江本利,於春,王红娟,等.安徽凤丹资源应用扩展的形势分析与策略探讨[J].安徽农业科学,2016,44(35):222～223,227.