乳酸菌胞外多糖对逆境胁迫下水稻种子萌发及幼苗生长的影响
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摘要
为探究乳酸菌胞外多糖浸种对酸、盐胁迫条件下水稻生长发育的影响,采用室内恒温培养试验,研究不同浓度乳酸菌胞外多糖对水稻种子萌发及相关生理代谢指标的影响。结果表明,采用不同浓度胞外多糖浸种可以显著提高逆境胁迫下水稻种子的发芽势、发芽率及发芽指数,促进水稻根系生长,随着胞外多糖浓度的升高,对水稻种子萌发的促进作用呈先升高后降低趋势;胞外多糖浓度为200 mg·L~(-1)时,缓解逆境胁迫伤害效果最为显著;pH值为3.0的酸胁迫下,与不添加胞外多糖相比,水稻种子的发芽势、发芽率及发芽指数分别提高了178.2%、38.7%和114.4%;7 mg·mL~(-1)NaCl胁迫下,发芽势、发芽率、发芽指数分别提高了152.9%、73.8%、103.0%,且差异均达显著水平(P<0.05);胞外多糖浸种可显著降低各胁迫下水稻幼芽丙二醛(MDA)含量,不同程度地提高过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)等抗氧化酶活性,同时胞外多糖对经过逆境胁迫的水稻种子播种后生长的幼苗也具有一定的促进作用,可缓解逆境胁迫对水稻造成的毒害。本研究为微生物源物增强作物抗逆性研究提供了理论依据。
In order to investigate effect of exopolysaccharides(EPS) of lactic acid bacteria on the germination of rice seeds and physiological indexes of rice under acid and salt stress, different concentrations of EPS of lactic acid bacteria was applied to rice seed in their germination stage. The results indicated that the EPS can significantly increase seed germination potential, germination rate and germination index, root growth were also increased significantly. As the the EPS concentration increased, the trend of rice seed germination increased firstly and then decreased under different stress. The content of 200 mg·L~(-1) EPS significantly alleviated the damage caused by acid and salt stress. Specifically, germination potential, germination rate and germination index were increased by 178.2%, 38.7% and 114.4% under acid stress, respectively. Under salt(7 mg·L~(-1)NaCl) stress, compared with without EPS, germination potential, germination rate and germination index were also increased by 152.9%, 73.8% and 103.0%, respectively. Their difference reached at a significant level(P<0.05). Further analysis showed that the process of soaking in EPS would reduce malondiadehyde(MDA)content and enhance the activity of antioxidant enzymes in varying degree such as catalase(CAT) activity, peroxidase(POD) activity, superoxide dismutase(SOD) activity of rice shoots, which could effectively alleviate the toxic effects of stress resistance. At the same time, EPS could also promote the growth of rice seedlings under stress. This study provides theoretical and practice foundation for improving crops stress resistance by microbe-derived.
In order to investigate effect of exopolysaccharides(EPS) of lactic acid bacteria on the germination of rice seeds and physiological indexes of rice under acid and salt stress, different concentrations of EPS of lactic acid bacteria was applied to rice seed in their germination stage. The results indicated that the EPS can significantly increase seed germination potential, germination rate and germination index, root growth were also increased significantly. As the the EPS concentration increased, the trend of rice seed germination increased firstly and then decreased under different stress. The content of 200 mg·L~(-1) EPS significantly alleviated the damage caused by acid and salt stress. Specifically, germination potential, germination rate and germination index were increased by 178.2%, 38.7% and 114.4% under acid stress, respectively. Under salt(7 mg·L~(-1)NaCl) stress, compared with without EPS, germination potential, germination rate and germination index were also increased by 152.9%, 73.8% and 103.0%, respectively. Their difference reached at a significant level(P<0.05). Further analysis showed that the process of soaking in EPS would reduce malondiadehyde(MDA)content and enhance the activity of antioxidant enzymes in varying degree such as catalase(CAT) activity, peroxidase(POD) activity, superoxide dismutase(SOD) activity of rice shoots, which could effectively alleviate the toxic effects of stress resistance. At the same time, EPS could also promote the growth of rice seedlings under stress. This study provides theoretical and practice foundation for improving crops stress resistance by microbe-derived.
引文
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[4] 王佳丽, 黄贤金, 钟太洋, 陈志刚. 盐碱地可持续利用研究综述[J]. 地理学报, 2011,66(5):673-684
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[9] Sandhya Ⅴ, Ali S Z, Grover M, Reddy G, Venkateswarlu B. Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45[J]. Biology and Fertility of Soils, 2009,46(1):17-26
[10] 马纯艳, 卜宁, 马连菊. 褐藻胶寡糖对高粱种子萌发及幼苗生理特性的影响[J]. 沈阳师范大学学报(自然科学版), 2010,28(1):79-82
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[13] Huang Z M, Cai K T, Shen Q W, Zhou H M. Progress of research on stress resistance of rice in China[J]. Agricultural Science & Technology, 2012,13(12):2529-2533
[14] Yang Z N, Li S Y, Zhang X, Zeng X P, Li D, Zhao Y J, Zhang J. Capsular and slime-polysaccharide production by Lactobacillus rhamnosus JAAS8 isolated from Chinese sauerkraut: potential application in fermented milk products[J]. Journal of Bioscience & Bioengineering, 2010, 110(1):53-57
[15] Rimada P S, Abranham A G. Comparative study of different methodologies to determine the exopolysaccharide produced by kefir grains in milk and whey[J]. Dairy Science & Technology, 2003, 83(1):79-87
[16] 张文平, 李昆太, 黄林, 魏塞金,程新. 产胞外多糖菌株的筛选及其 对土壤团聚体的影响[J]. 江西农业大学学报, 2017, 39(4):772-779
[17] 王学奎. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2006
[18] Zhang H J, Zhang N, Yang R C, Wang L, Sun Q Q, Li D B, Cao Y Y, Weeda S, Zhao B, Ren S,Guo Y D. Melatonin promotes seed germination under high salinity by regulating antioxidant systems, ABA and GA4 interaction in cucumber (Cucumis sativus L.)[J]. Journal of Pineal Research, 2014, 57(3):269-79
[19] 彭向永, 于荟, 石磊, 孔凡华. 海带硫酸多糖对镉毒害甜瓜幼苗的保护作用[J]. 农业环境科学学报, 2010, 29(9):1640-1645
[20] 毕锐. 昆虫多糖对水稻促生长增抗逆效果及其机理研究[D]. 长春:吉林农业大学, 2007
[21] Sandhya Ⅴ, Ali S Z. The production of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress conditions and its role in soil aggregation[J]. Microbiology, 2015,84(4):512-519
[22] Xu Y, Rossi F, Colica G, Colica G, Deng S Q, Philippis R D, Chen L Z. Use of cyanobacterial polysaccharides to promote shrub performances in desert soils: a potential approach for the restoration of desertified areas[J]. Biology and Fertility of Soils, 2013,49(2):143-152
[23] 刘偲嘉. PS04菌株胞外多糖诱导植物抗性及其应用研究[D]. 广州:华南农业大学, 2016
[24] 闫慧萍, 彭云玲, 赵小强, 吕玉燕. 外源24-表油菜素内酯对逆境胁迫下玉米种子萌发和幼苗生长的影响[J]. 核农学报, 2016, 30(5):988-996
[25] 刘爱荣, 张远兵, 陈登科. 盐胁迫对盐芥(Thellungiella halophila)生长和抗氧化酶活性的影响[J]. 植物研究, 2006, 26(2):216-221
[26] 刘仕翔, 黄益宗, 罗泽娇, 黄永春,保琼莉,王培培,袁彪,李文华. 外源褪黑素处理对镉胁迫下水稻种子萌发的影响[J]. 农业环境科学学报, 2016, 35(6):1034-1041
[27] 曹君, 王红, 齐鑫, 马骁颖,杨镇. 微生物多糖对草地早熟禾抗旱酶系及生理生化指标的影响[J]. 园艺与种苗, 2016(11):16-18
[28] Yang H X, Deng J J, Yuan Y, Fan D L, Zhang Y, Zhang R J, Han B. Two novel exopolysaccharides from Bacillus amyloliquefaciens C-1: antioxidation and effect on oxidative stress[J]. Current Microbiology, 2015, 70(2):298-306
[29] 杨小环,赵维峰,孙娜娜,孙亮亮,马金虎. 外源水杨酸缓解低温胁迫对玉米种子萌发和早期幼苗生长伤害的生理机制[J]. 核农学报,2017,31(9):1811-1817
[30] Turk H, Erdal S. Melatonin alleviates cold‐induced oxidative damage in maize seedlings by up-regulating mineral elements and enhancing antioxidant activity[J]. Journal of Plant Nutrition and Soil Science=Zeitschrift fuer Pflanzenernaehrung und Bodenkund, 2015,178(3):433-439
[31] 黄益宗, 隋立华. 臭氧污染胁迫下植物的抗氧化系统调节机制[J]. 生态毒理学报, 2013,8(4):456-464
[32] Bajguz A. An enhancing effect of exogenous brassinolide on the growth and antioxidant activity in Chlorella vulgaris cultures under heavy metals stress[J]. Environmental & Experimental Botany, 2010, 68(2):175-179
[33] Tewari S, Arora N K. Multifunctional exopolysaccharides from Pseudomonas aeruginosa, PF23 involved in plant growth stimulation, biocontrol and stress amelioration in sunflower under saline conditions[J]. Current Microbiology, 2014, 69(4):484-94
[34] 史静, 潘根兴, 夏运生, 张仕颖,张乃生. 镉胁迫对两品种水稻生长及抗氧化酶系统的影响[J]. 生态环境学报, 2013,22(5):832-837
[35] Wang J, Zhao X, Yang Y, Zhao A, Yang Z. Characterization and bioactivities of an exopolysaccharide produced by Lactobacillus plantarum YW32[J]. International Journal of Biological Macromolecules, 2015,74:119-126
[36] Haroun M B, Refaat M B, El-Menoufy A H, Amin A H, El-Waself A A. Structure analysis and antitumor activity of the exopolysaccharide from probiotic Lactobacillus plantarum NRRL B- 4496 in vitro and in vivo[J]. Journal of Applied Sciences Research, 2013,9(1):425-434
[37] Kang H, Choi H S, Kim J E, Han N S. Exopolysaccharide-overproducing Lactobacillus paracasei KB28 induces cytokines in mouse peritoneal macrophages via modulation of NF-κβ and MAPKs[J]. Journal of Microbiology & Biotechnology, 2011, 21(11):1174-1178
[38] 秦晓萌, 张远森, 柳陈坚,张昆林,杨恩. 乳酸菌胞外多糖生理功能及合成途径的研究进展[J]. 食品工业科技, 2015, 36(14):389-393
[39] 杨靖鹏, 王静, 张晓辉, 樊明涛,丁欢,魏新元. 乳酸菌胞外多糖研究进展以及在食品工业中的应用[J]. 食品与发酵工业, 2016, 42(1):264-272
[2] IPCC L P R K. Climate change 2014: synthesis report. contribution of working groups Ⅰ, Ⅱ and Ⅲ to the fifth assessment report of the intergovernmental panel on climate change[J]. Journal of Romance Studies, 2015,4(2):85-88
[3] 张秀, 张黎明, 龙军, 陈翰阅,范协裕,邢世和,徐福祥. 亚热带耕地土壤酸化程度差异及影响因素[J]. 中国生态农业学报, 2017,25(3):441-450
[4] 王佳丽, 黄贤金, 钟太洋, 陈志刚. 盐碱地可持续利用研究综述[J]. 地理学报, 2011,66(5):673-684
[5] 王志刚, 申红芳, 王磊. 我国水稻生产的特点与影响因素调查分析[J]. 中国稻米, 2010,16(1):26-29
[6] 解开治, 徐培智, 严超, 张发宝,陈建生,唐栓虎,黄旭,顾文杰. 不同土壤改良剂对南方酸性土壤的改良效果研究[J]. 中国农学通报, 2009, 25(20):160-165
[7] 陈琨, 秦鱼生, 喻华, 康思文,樊红柱,曾祥忠,涂仕华. 不同肥料/改良剂对冷泥田水稻生长、养分吸收及土壤性质的影响[J]. 植物营养与肥料学报, 2015, 21(3):773-781
[8] 邱德文. 植物免疫诱抗剂的研究进展与应用前景[J]. 中国农业科技导报, 2014,16(1):39-45
[9] Sandhya Ⅴ, Ali S Z, Grover M, Reddy G, Venkateswarlu B. Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45[J]. Biology and Fertility of Soils, 2009,46(1):17-26
[10] 马纯艳, 卜宁, 马连菊. 褐藻胶寡糖对高粱种子萌发及幼苗生理特性的影响[J]. 沈阳师范大学学报(自然科学版), 2010,28(1):79-82
[11] 闫亚南. 产胞外多糖植物促生菌对小麦高效利用钾肥的影响及机制研究[D]. 南京:南京农业大学, 2012
[12] 郭田, 王刘庆, 廖美德. PS04菌株对水稻纹枯病的防效及对水稻2种防御性酶活性的诱导[J]. 西北农林科技大学学报(自然科学版), 2013, 41(6):98-102
[13] Huang Z M, Cai K T, Shen Q W, Zhou H M. Progress of research on stress resistance of rice in China[J]. Agricultural Science & Technology, 2012,13(12):2529-2533
[14] Yang Z N, Li S Y, Zhang X, Zeng X P, Li D, Zhao Y J, Zhang J. Capsular and slime-polysaccharide production by Lactobacillus rhamnosus JAAS8 isolated from Chinese sauerkraut: potential application in fermented milk products[J]. Journal of Bioscience & Bioengineering, 2010, 110(1):53-57
[15] Rimada P S, Abranham A G. Comparative study of different methodologies to determine the exopolysaccharide produced by kefir grains in milk and whey[J]. Dairy Science & Technology, 2003, 83(1):79-87
[16] 张文平, 李昆太, 黄林, 魏塞金,程新. 产胞外多糖菌株的筛选及其 对土壤团聚体的影响[J]. 江西农业大学学报, 2017, 39(4):772-779
[17] 王学奎. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2006
[18] Zhang H J, Zhang N, Yang R C, Wang L, Sun Q Q, Li D B, Cao Y Y, Weeda S, Zhao B, Ren S,Guo Y D. Melatonin promotes seed germination under high salinity by regulating antioxidant systems, ABA and GA4 interaction in cucumber (Cucumis sativus L.)[J]. Journal of Pineal Research, 2014, 57(3):269-79
[19] 彭向永, 于荟, 石磊, 孔凡华. 海带硫酸多糖对镉毒害甜瓜幼苗的保护作用[J]. 农业环境科学学报, 2010, 29(9):1640-1645
[20] 毕锐. 昆虫多糖对水稻促生长增抗逆效果及其机理研究[D]. 长春:吉林农业大学, 2007
[21] Sandhya Ⅴ, Ali S Z. The production of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress conditions and its role in soil aggregation[J]. Microbiology, 2015,84(4):512-519
[22] Xu Y, Rossi F, Colica G, Colica G, Deng S Q, Philippis R D, Chen L Z. Use of cyanobacterial polysaccharides to promote shrub performances in desert soils: a potential approach for the restoration of desertified areas[J]. Biology and Fertility of Soils, 2013,49(2):143-152
[23] 刘偲嘉. PS04菌株胞外多糖诱导植物抗性及其应用研究[D]. 广州:华南农业大学, 2016
[24] 闫慧萍, 彭云玲, 赵小强, 吕玉燕. 外源24-表油菜素内酯对逆境胁迫下玉米种子萌发和幼苗生长的影响[J]. 核农学报, 2016, 30(5):988-996
[25] 刘爱荣, 张远兵, 陈登科. 盐胁迫对盐芥(Thellungiella halophila)生长和抗氧化酶活性的影响[J]. 植物研究, 2006, 26(2):216-221
[26] 刘仕翔, 黄益宗, 罗泽娇, 黄永春,保琼莉,王培培,袁彪,李文华. 外源褪黑素处理对镉胁迫下水稻种子萌发的影响[J]. 农业环境科学学报, 2016, 35(6):1034-1041
[27] 曹君, 王红, 齐鑫, 马骁颖,杨镇. 微生物多糖对草地早熟禾抗旱酶系及生理生化指标的影响[J]. 园艺与种苗, 2016(11):16-18
[28] Yang H X, Deng J J, Yuan Y, Fan D L, Zhang Y, Zhang R J, Han B. Two novel exopolysaccharides from Bacillus amyloliquefaciens C-1: antioxidation and effect on oxidative stress[J]. Current Microbiology, 2015, 70(2):298-306
[29] 杨小环,赵维峰,孙娜娜,孙亮亮,马金虎. 外源水杨酸缓解低温胁迫对玉米种子萌发和早期幼苗生长伤害的生理机制[J]. 核农学报,2017,31(9):1811-1817
[30] Turk H, Erdal S. Melatonin alleviates cold‐induced oxidative damage in maize seedlings by up-regulating mineral elements and enhancing antioxidant activity[J]. Journal of Plant Nutrition and Soil Science=Zeitschrift fuer Pflanzenernaehrung und Bodenkund, 2015,178(3):433-439
[31] 黄益宗, 隋立华. 臭氧污染胁迫下植物的抗氧化系统调节机制[J]. 生态毒理学报, 2013,8(4):456-464
[32] Bajguz A. An enhancing effect of exogenous brassinolide on the growth and antioxidant activity in Chlorella vulgaris cultures under heavy metals stress[J]. Environmental & Experimental Botany, 2010, 68(2):175-179
[33] Tewari S, Arora N K. Multifunctional exopolysaccharides from Pseudomonas aeruginosa, PF23 involved in plant growth stimulation, biocontrol and stress amelioration in sunflower under saline conditions[J]. Current Microbiology, 2014, 69(4):484-94
[34] 史静, 潘根兴, 夏运生, 张仕颖,张乃生. 镉胁迫对两品种水稻生长及抗氧化酶系统的影响[J]. 生态环境学报, 2013,22(5):832-837
[35] Wang J, Zhao X, Yang Y, Zhao A, Yang Z. Characterization and bioactivities of an exopolysaccharide produced by Lactobacillus plantarum YW32[J]. International Journal of Biological Macromolecules, 2015,74:119-126
[36] Haroun M B, Refaat M B, El-Menoufy A H, Amin A H, El-Waself A A. Structure analysis and antitumor activity of the exopolysaccharide from probiotic Lactobacillus plantarum NRRL B- 4496 in vitro and in vivo[J]. Journal of Applied Sciences Research, 2013,9(1):425-434
[37] Kang H, Choi H S, Kim J E, Han N S. Exopolysaccharide-overproducing Lactobacillus paracasei KB28 induces cytokines in mouse peritoneal macrophages via modulation of NF-κβ and MAPKs[J]. Journal of Microbiology & Biotechnology, 2011, 21(11):1174-1178
[38] 秦晓萌, 张远森, 柳陈坚,张昆林,杨恩. 乳酸菌胞外多糖生理功能及合成途径的研究进展[J]. 食品工业科技, 2015, 36(14):389-393
[39] 杨靖鹏, 王静, 张晓辉, 樊明涛,丁欢,魏新元. 乳酸菌胞外多糖研究进展以及在食品工业中的应用[J]. 食品与发酵工业, 2016, 42(1):264-272