亚慢性铜暴露对背角无齿蚌鳃抗氧化系统的影响
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摘要
为探究淡水环境中铜(Copper, Cu)污染对背角无齿蚌(Anodonta woodiana)鳃抗氧化系统的影响,检测了Cu~(2+)(0.137、0.548、2.192 mg·L~(-1))暴露于背角无齿蚌7、14、21、28 d后,其鳃中超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GPx)和谷胱甘肽转硫酶(Glutathione-S-transferase,GST)的活性,还原型谷胱甘肽(Reduced glutathione,GSH)和丙二醛(Malondialdehyde,MDA)的含量及总抗氧化能力(Total antioxidant capacity,TAOC)的变化。结果显示:随着Cu~(2+)浓度的增加和暴露时间的延长,MDA含量逐渐上升,表现出明显的"剂量-效应"和"时间-效应"关系;抗氧化酶SOD、GPx和GST活性均被显著诱导(P<0.05), CAT活性被显著抑制(P<0.05),抗氧化剂GSH含量显著降低(P<0.05);Cu~(2+)暴露14、21、28 d,T-AOC与对照组相比均显著升高(P<0.05)。总体而言,背角无齿蚌鳃抗氧化系统在Cu~(2+)暴露下被激活,但是鳃组织器官仍然受到了氧化损伤。GPx、GST和GSH对Cu~(2+)暴露响应最为灵敏。
To explore the effect of freshwater copper(Cu)pollution on the antioxidant system in the gills of the mussel Anodonta woodiana,the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), and glutathione S-transferase(GST), and the contents of reduced glutathione(GSH), and malondialdehyde(MDA), and total antioxidant capacity(T-AOC)in mussel gills exposed to Cu~(2+)(0.137, 0.548, 2.192 mg·L~(-1))for 7, 14, 21 and 28 d, were tested. The results showed that:MDA content increased with increased Cu~(2+)concentration and extended exposure time, showing"dose-effect"and"time-effect"relationships. With Cu~(2+)exposure, the activity of antioxidant enzymes SOD, GPx, and GST were significantly induced(P<0.05), while the activity of CAT was significantly inhibited(P<0.05),in comparison to the control group. In addition, the content of the antioxidant GSH decreased significantly(P<0.05). T-AOC was significantly higher(P<0.05)than the control group after exposure to Cu~(2+) for 14, 21 and 28 d. Overall, the antioxidant system in gills of A. woodiana was activated under Cu~(2+) exposure, but the gills still suffered oxidative damage. In addition, the responses of GPx, GST, and GSH to Cu~(2+) exposure were the most sensitive among the indicators detected in this study, which could provide a scientific basis for using A. woodiana to monitor and assess freshwater copper pollution.
To explore the effect of freshwater copper(Cu)pollution on the antioxidant system in the gills of the mussel Anodonta woodiana,the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), and glutathione S-transferase(GST), and the contents of reduced glutathione(GSH), and malondialdehyde(MDA), and total antioxidant capacity(T-AOC)in mussel gills exposed to Cu~(2+)(0.137, 0.548, 2.192 mg·L~(-1))for 7, 14, 21 and 28 d, were tested. The results showed that:MDA content increased with increased Cu~(2+)concentration and extended exposure time, showing"dose-effect"and"time-effect"relationships. With Cu~(2+)exposure, the activity of antioxidant enzymes SOD, GPx, and GST were significantly induced(P<0.05), while the activity of CAT was significantly inhibited(P<0.05),in comparison to the control group. In addition, the content of the antioxidant GSH decreased significantly(P<0.05). T-AOC was significantly higher(P<0.05)than the control group after exposure to Cu~(2+) for 14, 21 and 28 d. Overall, the antioxidant system in gills of A. woodiana was activated under Cu~(2+) exposure, but the gills still suffered oxidative damage. In addition, the responses of GPx, GST, and GSH to Cu~(2+) exposure were the most sensitive among the indicators detected in this study, which could provide a scientific basis for using A. woodiana to monitor and assess freshwater copper pollution.
引文
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[2]Chan C Y,Wang W X.A lipidomic approach to understand copper resilience in oyster Crassostrea hongkongensis[J].Aquatic Toxicology,2018,204:160-170.
[3]Boukadida K,Cachot J,Clérandeaux C,et al.Early and efficient induction of antioxidant defense system in Mytilus galloprovincialis embryos exposed to metals and heat stress[J].Ecotoxicology and Environmental Safety,2017,138:105-112.
[4]Xie Y H,Chen H B,Zheng S Y,et al.Molecular characterization of Cu/Zn SOD gene in Asian clam Corbicula fluminea and mRNA expression and enzymatic activity modulation induced by metals[J].Gene,2018,663:189-195.
[5]刘新,蒋豫,高俊峰,等.巢湖湖区及主要出入湖河流表层沉积物重金属污染特征及风险评价[J].湖泊科学,2016,28(3):502-512.LIU Xin,JIANG Yu,GAO Jun-feng,et al.Pollution characteristics of heavy metals and the risk assessment for the surface sediments from Lake Chaohu and its main tributary rivers[J].Journal of Lake Sciences,2016,28(3):502-512.
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[7]李传琼,王鹏,陈波,等.鄱阳湖流域赣江水系溶解态金属元素空间分布特征及污染来源[J].湖泊科学,2018,30(1):139-149.LI Chuan-qiong,WANG Peng,CHEN Bo,et al.Spatial distribution and pollution source of dissolved metals in the Ganjiang River of Lake Poyang Basin[J].Journal of Lake Sciences,2018,30(1):139-149.
[8]Rachel L R,Andrew C J,Claudia M,et al.Using risk-ranking of metals to identify which poses the greatest threat to freshwater organisms in the UK[J].Environmental Pollution,2014,194:17-23.
[9]王振,金小伟,王子健.铜对水生生物的毒性:类群特异性敏感度分析[J].生态毒理学报,2014,9(4):640-646.WANG Zhen,JIN Xiao-wei,WANG Zi-jian.Taxon-specific sensitivity differences of copper to aquatic organisms[J].Asian Journal of Ecotoxicology,2014,9(4):640-646.
[10]Shoults-Wilson W A,Elsayed N,Leckrone K,et al.Zebra mussels(Dreissena polymorpha)as a biomonitor of trace elements along the southern shoreline of Lake Michigan[J].Environmental Toxicology and Chemistry,2015,34(2):412-419.
[11]Xia X C,Huang C F,Zhang D X,et al.Molecular cloning,characterization,and the response of Cu/Zn superoxide dismutase and catalase to PBDE-47 and-209 from the freshwater bivalve Anodonta woodiana[J].Fish&Shellfish Immunology,2016,51:200-210.
[12]Falfushynska H I,Gnatyshyna L L,Stoliar O B.Effect of in situ exposure history on the molecular responses of freshwater bivalve Anodonta anatina(Unionidae)to trace metals[J].Ecotoxicology and Environmental Safety,2013,89:73-83.
[13]Molnar N,Fong P P.Toxic effects of copper,cadmium,and methoxychlor shown by neutral red retention assay in two species of freshwater molluscs[J].The Open Environmental Pollution&Toxicology Journal,2012,3(3):65-71.
[14]Kumar N,Krishnani K K,Meena K K,et al.Oxidative and cellular metabolic stress of Oreochromis mossambicus as biomarkers indicators of trace element contaminants[J].Chemosphere,2017,171:265-274.
[15]Guo R M,Pan L Q,Ji R W.A multi-biomarker approach in scallop Chlamys farreri to assess the impact of contaminants in Qingdao coastal area of China[J].Ecotoxicology and Environmental Safety,2017,142:399-409.
[16]孙磊,陈修报,苏彦平,等.东湖移殖背角无齿蚌中重金属的含量变化[J].水生生物学报,2014,38(1):203-208.SUN Lei,CHEN Xiu-bao,SU Yan-ping,et al.Variation of heavy metal contents in swan mussels(Anodonta woodiana)transplanted into Donghu Lake[J].Acta Hydrobiologica Sinica,2014,38(1):203-208.
[17]陈修报,苏彦平,刘洪波,等.基于“标准化”背角无齿蚌(Anodonta woodiana)对太湖五里湖重金属污染的主动监测[J].湖泊科学,2014,26(6):857-863.CHEN Xiu-bao,SU Yan-ping,LIU Hong-bo,et al.Active biomonitoring of heavy metal pollution level of Wulihu area,Lake Taihu using“standardized”Anodonta woodiana[J].Journal of Lake Sciences,2014,26(6):857-863.
[18]袁牧,王昌留,王一斐,等.超氧化物歧化酶的进展[J].中国组织化学与细胞化学杂志,2016,25(6):550-554.YUAN Mu,WANG Chang-liu,WANG Yi-fei,et al.Progress in the research of superoxide dismutase[J].Chinese Journal of Histochemistry and Cytochemistry,2016,25(6):550-554.
[19]Bebianno M J,Mello A C P,Serrano M A S,et al.Transcriptional and cellular effects of paracetamol in the oyster Crassostrea gigas[J].Ecotoxicology and Environmental Safety,2017,144:258-267.
[20]Kumar N,Krishnani K K,Singh N P.Oxidative and cellular stress as bioindicators for metal contamination in freshwater mollusk Lamellidens marginalis[J].Environmental Science and Pollution Research International,2017,24(19):16137-16147.
[21]Li Y F,Gu Z Q,Liu H,et al.Biochemical response of the mussel Mytilus coruscus(Mytiloida:Mytilidae)exposed to in vivo sub-lethal copper concentrations[J].Chinese Journal of Oceanology and Limnology,2012,30(5):738-745.
[22]Netpae T,Phalaraksh C,Wongkham W.Antioxidant enzyme activities and DNA damage as biomarker of copper effect on Corbicula fluminea[J].Electronic Journal of Biology,2012,8(2):19-23.
[23]Rossi F,Palombella S,Pirrone C,et al.Evaluation of tissue morphology and gene expression as biomarkers of pollution in mussel Mytilus galloprovincialis caging experiment[J].Aquatic Toxicology,2016,181:57-66.
[24]Wu J,Bao M M,Ge D L,et al.The expression of superoxide dismutase in Mytilus coruscus under various stressors[J].Fish&Shellfish Immunology,2017,70:361-371.
[25]Macías-Mayorga D,Laiz I,Moreno-Garrido I,et al.Is oxidative stress related to cadmium accumulation in the Mollusc Crassostrea angulata?[J].Aquatic Toxicology,2015,161:231-241.
[26]孟晓林,田雪,郭倩倩,等.铜在虾夷扇贝鳃和肝胰腺中的累积及对抗氧化酶、脂代谢的影响[J].水产学报,2015,39(6):908-913.MENG Xiao-lin,TIAN Xue,GUO Qian-qian,et al.Copper specific accumulation and its effect on the activity of antioxidant enzyems and metabolism of lipid in gill or hepatopancreas of Patinopecten yessoensis[J].Journal of Fisheries of China,2015,39(6):908-913.
[27]Xia L P,Chen S H,Dahms H U,et al.Cadmium induced oxidative damage and apoptosis in the hepatopancreas of Meretrix meretrix[J].Ecotoxicology,2016,25(5):959-969.
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