青海不同地区麻花艽地上部位有效成分的含量测定
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摘要
为筛选麻花艽的最佳优良产区,结合聚类分析和主成分分析,采用HPLC方法比较海拔2 500～3 800m的青海15个地区麻花艽含量的差异。采用Hypersil ODS(4.6 mm×250 mm,5μm)色谱柱,以含0.04%磷酸的水溶液—甲醇为流动相,梯度洗脱,流速1 m L/min,检测波长254 nm,柱温35℃。根据分析结果,初步筛选出麻花艽的优良产区为青海河南县和同仁县。该方法简便、可靠,可为青海选取麻花艽优良产区、建立麻花艽的人工种植基地提供参考。
In order to screen the best production areas of Gentiana straminea Maxim,combined the cluster analysis with the principal component analysis,in this work,the content differences of Gentiana straminea Maxim in 15 regions of Qinghai province at altitudes of 2 500 ～ 3 800 m were systematically assessed and compared by HPLC. The Hypersil ODS( 4.6 mm×250 mm,5 micron) column was used with methanol-water containing 0.04%phosphoric acid as the mobile phase,with gradient elution,flow rate of 1 m L/min,detection of 254 nm,and column temperature of 35 ℃. According to our analysis results,the excellent production areas of Gentiana straminea Maxim were preliminarily found to be in Henan and Tongren counties in Qinghai province. Our methodology is simple and reliable,and can provide reliable references for the selection of excellent productions areas of Gentiana straminea Maxim and trustworthy guidelines for the establishment of artificial planting bases of Gentiana straminea Maxim in Qinghai province.
In order to screen the best production areas of Gentiana straminea Maxim,combined the cluster analysis with the principal component analysis,in this work,the content differences of Gentiana straminea Maxim in 15 regions of Qinghai province at altitudes of 2 500 ～ 3 800 m were systematically assessed and compared by HPLC. The Hypersil ODS( 4.6 mm×250 mm,5 micron) column was used with methanol-water containing 0.04%phosphoric acid as the mobile phase,with gradient elution,flow rate of 1 m L/min,detection of 254 nm,and column temperature of 35 ℃. According to our analysis results,the excellent production areas of Gentiana straminea Maxim were preliminarily found to be in Henan and Tongren counties in Qinghai province. Our methodology is simple and reliable,and can provide reliable references for the selection of excellent productions areas of Gentiana straminea Maxim and trustworthy guidelines for the establishment of artificial planting bases of Gentiana straminea Maxim in Qinghai province.
引文
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[9]杨燕梅,林丽,卢有媛,等.基于多指标成分分析野生与栽培秦艽药材商品规格等级[J].中国中药杂志,2016,41(5):786-792.
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[12]李永平,李向阳,田丰,等.青海境内麻花艽野生种质资源调查研究[J].甘肃中医学院学报,2013,30(3):71-75.
[13]黄鹤飞,刘红兵,王燕燕,等. HPLC法测定板蓝根药材及农本方颗粒中表告依春的含量[J].湖南师范大学学报(医学版),2014,11(2):46-48.
[14]柳贤福,黄红泓,覃日宏,等.白苞蒿提取工艺优化及槲皮素含量测定[J].湖南师范大学自然科学学报,2018,41(5):43-47.
[15]陈文,杜明月,刘浩然.高效液相色谱法测定木姜叶柯水提取物中根皮苷的含量[J].湖南师范大学学报(医学版),2017,14(2):190-192.
[16]邝俊维,卿萍,周子虬,等. HPLC法同时测定白术中白术内酯Ⅰ,Ⅱ,Ⅲ的含量[J].湖南师范大学自然科学学报,2017,40(6):55-60.
[2]张军文,李成思,卢永昌,等. MEKC测定麻花艽中齐墩果酸和熊果酸含量[J].广西师范大学学报(自然科学版),2018,36(1):99-104.
[3]郑姜彬.麻花秦艽的化学成分研究[D].西安:西北大学,2010.
[4]帝玛尔·丹增彭措.晶珠本草[M].毛继祖译注.上海:上海科学技术出版社,1986.
[5]张莉,党军,梅丽娟,等.藏药麻花秦艽化学成分研究[J].中药材,2016,39(1):103-106.
[6]杨永昌.藏药志[S].西宁:青海人民出版社,1991:820.
[7]聂安政,林志健,王雨,等.秦艽化学成分及药理作用研究进展[J].中草药,2017,48(3):597-608.
[8]王爽,董红娇,王长生,等.不同产地麻花秦艽中5种环烯醚萜苷成分的含量比较[J].中国实验方剂学杂志,2015,21(8):77-80.
[9]杨燕梅,林丽,卢有媛,等.基于多指标成分分析野生与栽培秦艽药材商品规格等级[J].中国中药杂志,2016,41(5):786-792.
[10]王海涛,尹瑶,何涛.麻花艽愈伤组织培养过程中龙胆苦苷含量的变化[J].中药材,2014,37(3):372-374.
[11]聂颖杰,林鹏程. HPLC测定藏药麻花艽地上部位5种有效成分的含量[J].中国中药杂志,2010,35(10):1276-1279.
[12]李永平,李向阳,田丰,等.青海境内麻花艽野生种质资源调查研究[J].甘肃中医学院学报,2013,30(3):71-75.
[13]黄鹤飞,刘红兵,王燕燕,等. HPLC法测定板蓝根药材及农本方颗粒中表告依春的含量[J].湖南师范大学学报(医学版),2014,11(2):46-48.
[14]柳贤福,黄红泓,覃日宏,等.白苞蒿提取工艺优化及槲皮素含量测定[J].湖南师范大学自然科学学报,2018,41(5):43-47.
[15]陈文,杜明月,刘浩然.高效液相色谱法测定木姜叶柯水提取物中根皮苷的含量[J].湖南师范大学学报(医学版),2017,14(2):190-192.
[16]邝俊维,卿萍,周子虬,等. HPLC法同时测定白术中白术内酯Ⅰ,Ⅱ,Ⅲ的含量[J].湖南师范大学自然科学学报,2017,40(6):55-60.