激素处理茶树扦插生根效果的综合评价
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摘要
为了筛选适合茶树扦插育苗的激素处理方案、提高生根效果,采用正交试验方法和主成分分析法,研究激素种类、浓度及浸泡时间对‘鄂茶1号’茶树扦插生根的影响。结果表明,不同处理对茶树扦插生根率、根数、根长、根鲜重影响较大;3因素中,激素种类和激素浓度对扦插生根影响较大,浸泡时间影响相对较小;各处理扦插生根效果依次为处理Ⅰ(50 mg/kg的ABT1号浸泡插穗0.5 h)>处理Ⅴ(50 mg/kg的GGR10号浸泡插穗0.5 h)>处理Ⅳ(100 mg/kg的ABT1号浸泡插穗2 h)>处理Ⅵ(50 mg/kg的GGR10号浸泡插穗2 h)>处理Ⅱ(50 mg/kg的ABT1号浸泡插穗2 h)>处理Ⅷ(100 mg/kg的GGR10号浸泡插穗2 h)>处理Ⅲ(100 mg/kg的ABT1号浸泡插穗0.5 h)>处理Ⅶ(100 mg/kg的GGR10号浸泡插穗0.5 h),以50 mg/kg的ABT1号、GGR10号浸泡插穗0.5 h扦插效果最好。该处理方法为茶树规模化育苗提供理论依据,适合在生产中推广应用。
In order to select the suitable hormone treatment scheme for tea cutting and seedling growth, to improve the rooting effect. The effects of hormone species, concentration and soaking time on the root of tea cuttings of 'Echa 1'was studied by using orthogonal experimental design and principal component analysis. The results showed that different treatments had great effects on root rate, root number, root length and fresh root weight of tea cutting.Among the three factors, hormone type and concentration had greater influences on cutting propagation than soaking time. The order of rooting effect of each treatment was as follows: treatment Ⅰ(ABT1 50 mg/kg soak cuttings for 0.5 h)>treatment Ⅴ(GGR10 50 mg/kg soak cuttings for 0.5 h)>treatment Ⅳ(ABT1 100 mg/kg soak cuttings for2.0 h)>treatment Ⅵ(GGR10 50 mg/kg soak cuttings for 2.0 h)>treatment Ⅱ(ABT1 50 mg/kg soak cuttings for 2.0 h)>treatment Ⅷ(GGR10 100 mg/kg soak cuttings for 2.0 h)>treatment Ⅲ(ABT1 100 mg/kg soak cuttings for 0.5 h)>treatment Ⅶ(GGR10 10 mg/kg soak cuttings for 0.5 h). The cutting effect of soak cuttings with 50 mg/kg ABT1,GGR10 for 0.5 h was the best, which treatment method was suitable for popularization and application in production.
In order to select the suitable hormone treatment scheme for tea cutting and seedling growth, to improve the rooting effect. The effects of hormone species, concentration and soaking time on the root of tea cuttings of 'Echa 1'was studied by using orthogonal experimental design and principal component analysis. The results showed that different treatments had great effects on root rate, root number, root length and fresh root weight of tea cutting.Among the three factors, hormone type and concentration had greater influences on cutting propagation than soaking time. The order of rooting effect of each treatment was as follows: treatment Ⅰ(ABT1 50 mg/kg soak cuttings for 0.5 h)>treatment Ⅴ(GGR10 50 mg/kg soak cuttings for 0.5 h)>treatment Ⅳ(ABT1 100 mg/kg soak cuttings for2.0 h)>treatment Ⅵ(GGR10 50 mg/kg soak cuttings for 2.0 h)>treatment Ⅱ(ABT1 50 mg/kg soak cuttings for 2.0 h)>treatment Ⅷ(GGR10 100 mg/kg soak cuttings for 2.0 h)>treatment Ⅲ(ABT1 100 mg/kg soak cuttings for 0.5 h)>treatment Ⅶ(GGR10 10 mg/kg soak cuttings for 0.5 h). The cutting effect of soak cuttings with 50 mg/kg ABT1,GGR10 for 0.5 h was the best, which treatment method was suitable for popularization and application in production.
引文
Chen J.L.,Yun Y.,Yang L.R.,Wang C.M.,Yan W.P.,Chen X.,Weng C.Y.,and Zheng D.J.,2018,Effects of different exogenous hormones on rooting process of Camellia oleifera cuttings based on orthogonal experiment,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),16(10):3430-3440(陈加利,云勇,杨立荣,王春梅,严武平,陈宣,翁春雨,郑道君,2018,基于正交试验分析不同外源激素对油茶插穗生根的影响,分子植物育种,16(10):3430-3440)
De W.T.,Zhang R.Q.,and Yuan D.Y.,2011,Effect of plant growth regulators on rooting in Feijoa sellowiana,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),31(3):160-163(邓文韬,张日清,袁德义,2011,植物生长调节剂对菲油果嫩枝扦插生根的影响,中南林业科技大学学报,31(3):160-163)
Ding L.P.,Ma L.,Zhan Y.F.,and Tian X.P.,2017,Comprehensive evaluation of rooting effect of different hormone treatments on Lycium barbarum cuttings based on principal component analysis,Jingjilin Yanjiu(Nonwood Forest Research),35(2):110-115(丁丽萍,马力,占玉芳,田晓萍,2017,基于主成分分析的不同激素处理下宁夏枸杞扦插生根效果的综合评价,经济林研究,35(2):110-115)
Husen A.,2012,Change of soluble sugars and enzymatic activities during adventitious rooting in cutting of Grewia optiva as affected by age of donor plants and auxin treatments,Am.J.Plant Physiol.,7(1):1-6
Li H.,Nie C.N.,Tang Q.,Huang L.,and Wang X.Y.,2016,Analysis of main influence factors of high density and efficient cutting technology in western Sichuan tea area,Henan Nongye Kexue(Journal of Henan Agricultural Sciences),45(5):45-51(李慧,聂枞宁,唐茜,黄亮,王馨语,2016,川西茶区高密高效扦插技术的主要影响因素分析,河南农业科学,45(5):45-51)
Li Y.,Yan F.,He W.Z.,and Bao H.S.,2012,Effect of different rooting agents on cutting propagation of Huiming baicha,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),24(6):988-991(李阳,严芳,何卫中,包焕盛,2012,不同生根剂对惠明白茶苗扦插的影响,浙江农业学报,24(6):988-991)
Luo C.,Liu J.M.,Huang X.L.,Li L.X.,Liu J.J.,and Xiong X.,2018,Effect of different substrates,growth and regulators and parts of cutting on cutting propagation of Cinnamomum migao,Sichuan Daxue Xuebao(Journal of Sichuan University(Natural Science Edition)),55(4):843-847(骆畅,刘济明,黄小龙,李丽霞,柳嘉佳,熊雪,2018,不同基质、生长调节剂及部位对米槁扦插生根的影响,四川大学学报(自然科学版),55(4):843-847)
Meng H.L.,Yang S.C.,Zhang W.,Su Y.L.,Lu B.Y.,and Long G.Q.,2014,Influence of different substrate and plant growth regulators on rooting of cutting of Marsdenia tenacissima,Yunnan Nongye Daxue Xuebao(Journal of Yunnan Agricultural University),29(4):540-543(孟衡玲,杨生超,张薇,苏一兰,卢丙越,龙光强,2014,不同基质和激素对通关藤扦插生根的影响,云南农业大学学报,29(4):540-543)
Qu F.X.,Liu Y.Q.,Wu G.R.,and Chen C.L.,2009,Preliminary studying on softwood cutting propagation of Melaleuca bracteata,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(5):1006-1009(曲芬霞,刘玉清,吴桂容,陈春岚,2009,千层金嫩枝扦插繁殖技术,基因组学与应用生物学,28(5):1006-1009)
Su Z.N.,Chen S.W.,Yang J.H.,Tan L.,Luo Y.Z.,and Yang M.,2013,Effect of rooting agents on root morphology of cuttings of Chinese fir clone,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(6):787-794(苏治南,陈诗文,杨家鸿,谭玲,罗扬卓,杨梅,2013,不同生根剂对杉木扦插根形态建成的影响,基因组学与应用生物学,32(6):787-794)
Wang S.S.,Li X.H.,Zhang L.H.,Wang Z.H.,Shan W.,and Wang K.H.,2014,Effect of hormone types and concentrations on cutting propagation of Rhododendron latoucheae and its evatuation,Guangxi Zhiwu(Guihaia),34(2):227-234(王书胜,李晓花,张乐华,王兆红,单文,王凯红,2014,激素种类与浓度对鹿角杜鹃扦插繁殖的影响及其评价,广西植物,34(2):227-234)
Wu S.P.,Lv L.Z.,Zheng J.,Ren H.L.,Dang Y.C.,and Jiang S.F.,2014,Exploration of factors influencing survival rate of tea tree single node cutting,Henan Nongye Kexue(Journal of Henan Agricultural Sciences),43(10):34-37(吴淑平,吕立哲,郑杰,任红楼,党永超,蒋双丰,2014,茶树短穗扦插成活率的影响因素探析,河南农业科学,43(10):34-37)
Xu Y.,Yuan S.,Lu Y.F.,and Feng Z.J.,2017,Effect of plant growth regulators on cutting rooting of Machilus breviflora,Zhejiang Linye Keji(Journal of Zhejiang Forestry Science and Technology),37(5):69-73(徐茵,袁森,卢艺菲,冯志坚,2017,植物生长调节剂对短序润楠嫩枝扦插生根的影响,浙江林业科技,37(5):69-73)
Xue L.Y.,Kang Y.X.,Zhang D.,and Su Y.,2012,Influence factors on soft cutting propagation of Cornus wateri under full sunlight and water spra,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),40(11):10-13,18(薛利艳,康永祥,张丹,苏悦,2012,毛梾全光照喷雾嫩枝扦插繁殖试验,东北林业大学学报,40(11):10-13,18)
De W.T.,Zhang R.Q.,and Yuan D.Y.,2011,Effect of plant growth regulators on rooting in Feijoa sellowiana,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),31(3):160-163(邓文韬,张日清,袁德义,2011,植物生长调节剂对菲油果嫩枝扦插生根的影响,中南林业科技大学学报,31(3):160-163)
Ding L.P.,Ma L.,Zhan Y.F.,and Tian X.P.,2017,Comprehensive evaluation of rooting effect of different hormone treatments on Lycium barbarum cuttings based on principal component analysis,Jingjilin Yanjiu(Nonwood Forest Research),35(2):110-115(丁丽萍,马力,占玉芳,田晓萍,2017,基于主成分分析的不同激素处理下宁夏枸杞扦插生根效果的综合评价,经济林研究,35(2):110-115)
Husen A.,2012,Change of soluble sugars and enzymatic activities during adventitious rooting in cutting of Grewia optiva as affected by age of donor plants and auxin treatments,Am.J.Plant Physiol.,7(1):1-6
Li H.,Nie C.N.,Tang Q.,Huang L.,and Wang X.Y.,2016,Analysis of main influence factors of high density and efficient cutting technology in western Sichuan tea area,Henan Nongye Kexue(Journal of Henan Agricultural Sciences),45(5):45-51(李慧,聂枞宁,唐茜,黄亮,王馨语,2016,川西茶区高密高效扦插技术的主要影响因素分析,河南农业科学,45(5):45-51)
Li Y.,Yan F.,He W.Z.,and Bao H.S.,2012,Effect of different rooting agents on cutting propagation of Huiming baicha,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),24(6):988-991(李阳,严芳,何卫中,包焕盛,2012,不同生根剂对惠明白茶苗扦插的影响,浙江农业学报,24(6):988-991)
Luo C.,Liu J.M.,Huang X.L.,Li L.X.,Liu J.J.,and Xiong X.,2018,Effect of different substrates,growth and regulators and parts of cutting on cutting propagation of Cinnamomum migao,Sichuan Daxue Xuebao(Journal of Sichuan University(Natural Science Edition)),55(4):843-847(骆畅,刘济明,黄小龙,李丽霞,柳嘉佳,熊雪,2018,不同基质、生长调节剂及部位对米槁扦插生根的影响,四川大学学报(自然科学版),55(4):843-847)
Meng H.L.,Yang S.C.,Zhang W.,Su Y.L.,Lu B.Y.,and Long G.Q.,2014,Influence of different substrate and plant growth regulators on rooting of cutting of Marsdenia tenacissima,Yunnan Nongye Daxue Xuebao(Journal of Yunnan Agricultural University),29(4):540-543(孟衡玲,杨生超,张薇,苏一兰,卢丙越,龙光强,2014,不同基质和激素对通关藤扦插生根的影响,云南农业大学学报,29(4):540-543)
Qu F.X.,Liu Y.Q.,Wu G.R.,and Chen C.L.,2009,Preliminary studying on softwood cutting propagation of Melaleuca bracteata,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(5):1006-1009(曲芬霞,刘玉清,吴桂容,陈春岚,2009,千层金嫩枝扦插繁殖技术,基因组学与应用生物学,28(5):1006-1009)
Su Z.N.,Chen S.W.,Yang J.H.,Tan L.,Luo Y.Z.,and Yang M.,2013,Effect of rooting agents on root morphology of cuttings of Chinese fir clone,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(6):787-794(苏治南,陈诗文,杨家鸿,谭玲,罗扬卓,杨梅,2013,不同生根剂对杉木扦插根形态建成的影响,基因组学与应用生物学,32(6):787-794)
Wang S.S.,Li X.H.,Zhang L.H.,Wang Z.H.,Shan W.,and Wang K.H.,2014,Effect of hormone types and concentrations on cutting propagation of Rhododendron latoucheae and its evatuation,Guangxi Zhiwu(Guihaia),34(2):227-234(王书胜,李晓花,张乐华,王兆红,单文,王凯红,2014,激素种类与浓度对鹿角杜鹃扦插繁殖的影响及其评价,广西植物,34(2):227-234)
Wu S.P.,Lv L.Z.,Zheng J.,Ren H.L.,Dang Y.C.,and Jiang S.F.,2014,Exploration of factors influencing survival rate of tea tree single node cutting,Henan Nongye Kexue(Journal of Henan Agricultural Sciences),43(10):34-37(吴淑平,吕立哲,郑杰,任红楼,党永超,蒋双丰,2014,茶树短穗扦插成活率的影响因素探析,河南农业科学,43(10):34-37)
Xu Y.,Yuan S.,Lu Y.F.,and Feng Z.J.,2017,Effect of plant growth regulators on cutting rooting of Machilus breviflora,Zhejiang Linye Keji(Journal of Zhejiang Forestry Science and Technology),37(5):69-73(徐茵,袁森,卢艺菲,冯志坚,2017,植物生长调节剂对短序润楠嫩枝扦插生根的影响,浙江林业科技,37(5):69-73)
Xue L.Y.,Kang Y.X.,Zhang D.,and Su Y.,2012,Influence factors on soft cutting propagation of Cornus wateri under full sunlight and water spra,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),40(11):10-13,18(薛利艳,康永祥,张丹,苏悦,2012,毛梾全光照喷雾嫩枝扦插繁殖试验,东北林业大学学报,40(11):10-13,18)