百合地黄汤抗焦虑作用的研究
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摘要
目的:研究百合地黄汤改善焦虑的作用及作用机制。方法:雄性小鼠随机分为5组,即空白对照组,百合地黄汤高、中、低剂量组,地西泮组。连续灌胃给药14 d后,进行小鼠高架十字迷宫实验和开场实验的行为测试,用酶联免疫吸附法(ELISA)测定小鼠脑内γ-氨基丁酸(GABA)、谷氨酸(Glu)的含量。结果:和空白对照组比较,百合地黄汤高剂量组能增加小鼠在高架十字迷宫实验中进入开臂内运动时间和次数百分率,提高小鼠在开场试验中进入中央区的次数(P<0.05～0.01),表现出一定的抗焦虑作用;同时显示和空白对照组比较,百合地黄汤中、高剂量组小鼠脑组织内GABA含量增加、Glu的含量降低(P<0.05～0.01)。结论:百合地黄汤具有一定的抗焦虑作用,抗焦虑作用可能与提高小鼠脑内GABA含量、降低Glu的含量相关。
Objective: To study the effect of Baihe Dihuang Decoction in improving anxiety and its mechanism of action. Methods:Male mice were randomly divided into 5 groups: blank control group, Baihe Dihuang Decoction high, medium, low-dose group and diazepam group. After continuous intragastric administration for 14 days, the behavioral test of the mouse elevated plus maze experiment and opening test was performed. The brain tissue GABA and Glu content was measured by immunosorbent assay(ELISA). Results:Compared with the blank control group, the middle and high doses of Baihe Dihuang Decoction could increase the time and frequency of movement of the mice in the open cross labyrinth in the open arm and increase the number of times that the mouse entered the central area in the opening test(P<0.05, P<0.01). The anti-anxiety effect also showed an increase in the brain tissue GABA content in mice, ignificantly decreased Glu contents in mice(P<0.05, P<0.01). Conclusion:Baihe Dihuang Decoction has some anxiolytic effect, and anti-anxiety effect may be related to increasing brain tissue GABA content,decreased Glu contents in mice.
Objective: To study the effect of Baihe Dihuang Decoction in improving anxiety and its mechanism of action. Methods:Male mice were randomly divided into 5 groups: blank control group, Baihe Dihuang Decoction high, medium, low-dose group and diazepam group. After continuous intragastric administration for 14 days, the behavioral test of the mouse elevated plus maze experiment and opening test was performed. The brain tissue GABA and Glu content was measured by immunosorbent assay(ELISA). Results:Compared with the blank control group, the middle and high doses of Baihe Dihuang Decoction could increase the time and frequency of movement of the mice in the open cross labyrinth in the open arm and increase the number of times that the mouse entered the central area in the opening test(P<0.05, P<0.01). The anti-anxiety effect also showed an increase in the brain tissue GABA content in mice, ignificantly decreased Glu contents in mice(P<0.05, P<0.01). Conclusion:Baihe Dihuang Decoction has some anxiolytic effect, and anti-anxiety effect may be related to increasing brain tissue GABA content,decreased Glu contents in mice.
引文
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12 管家齐,孙燕,陈海伟. 百合地黄汤对小鼠抑郁症模型的影响[J] 中华中医药杂2013, 28(6):1875-1877.
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14 蒋征奎,李晓.百合地黄汤水提物和醇提物的抗抑郁作用对比[J].中医研究,2015,28(8):55-57.
15 徐淑云, 卞如濂, 陈修. 药理实验方法学[M].3版. 北京: 人民卫生出版社, 2003: 802-806.
16 刘小河,周平,孙大定,等.温胆片对高架十字迷宫实验小鼠的抗焦虑作用[J].中药新药与临床药理,2012,23(5):519-521.
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18 孟登科,周静,楼建秀,等.清宫汤抗焦虑作用实验研究[J].浙江中西医结合杂志2014,24(1):18-20.
19 郭花玲,崔瑛,李阳阳,等.黄连对小鼠焦虑行为影响的研究[J].中国实验方剂学杂志,2011,17(15):160-172.
20 刘挺.百合地黄汤的临床应用[J].内蒙古中医药,2014,22(5):94-96.
21 梁来德.百合地黄汤新用[J].现代中西药结合杂志,2010,19(4):481.
22 Li RZ,Guo JY,Li CY,et al.Progress of the research in GABAreceptors and anxiety disorders: Mechanism and pharmaceuticalintervertien[J]. Chin Pharmacoll Bull,2010,26(9) : 989-994.
23 Huang MP,Radadia K,Macone BW, et al.Effects of eszopiclone and zolpidem on sleep-wake behavior, anxiety-like behavior and contextual memory in rats[J]. Behavioural Brain Research, 2010, 210(1): 54-66.
24 Sorra K, Chen CS, Chang CF, et al. Synthesis, anticonvulsant, sedative and anxiolytic activities of novel annulated pyrrolo [1,4] benzodiazepines[J]. International Journal of Molecular Sciences, 2014, 15(9): 16500-16510.
25 王春梅,李贺,孙靖辉,等.北五味子多糖抗焦虑和镇静催眠作用[J].食品科学 2015,36(13):239-242.
2 龚绍麟. 抑郁症[M].北京: 人民卫生出版社,2003.
3 赵志刚,张星虎,张石革,等.当代神经精神科用药选择[M].北京: 人民卫生出版社,2003: 45.
4 王韵,杨福中,吴彦,等.焦虑障碍治疗药物现状[J] .上海医药2012,33(17):3-6.
5 李贺,李宁,刘聪,等.五味子素对ICR小鼠的抗焦虑作用及其作用机制[J].吉林大学学报(医学版)2018,44(1): 78-82.
6 肖炳坤,黄荣清,杨建云,等.抗焦虑天然药物研究进展[J].中国医院药学杂志2011,31(12):1021-1023.
7 刘菊,崔瑛,何丽果,等.百合抗焦虑物质提取工艺研究[J].中药材, 2009,32(1):134-136.
8 戴丽.百合地黄汤的临床应用和实验研究 [J]. 海峡药学2012,24(4):155-158.
9 王爱平,孙海波,冷辉. 百合地黄汤治疗耳鸣焦虑抑郁情绪障碍的疗效观察[J]. 内蒙古中医药 2014, 33(35): 34-35.
10 闫福庆.百合地黄汤加味治疗广泛性焦虑52例[J] .中国疗养医学,2004,13(3):151-152.
11 张景凤,仝桂兰,侯庆,等.加味百合地黄汤对中风后焦虑状态的临床疗效观察[J] .中草药,2005,36(5):737-738.
12 管家齐,孙燕,陈海伟. 百合地黄汤对小鼠抑郁症模型的影响[J] 中华中医药杂2013, 28(6):1875-1877.
13 刘小河,周平,孙大定,等.温胆片对高架十字迷宫实验小鼠的抗焦虑作用[J].中药新药与临床药理,2012,23(5):519-521.
14 蒋征奎,李晓.百合地黄汤水提物和醇提物的抗抑郁作用对比[J].中医研究,2015,28(8):55-57.
15 徐淑云, 卞如濂, 陈修. 药理实验方法学[M].3版. 北京: 人民卫生出版社, 2003: 802-806.
16 刘小河,周平,孙大定,等.温胆片对高架十字迷宫实验小鼠的抗焦虑作用[J].中药新药与临床药理,2012,23(5):519-521.
17 付治凤,王博龙,李伟,等.中药的抗焦虑作用机制研究进展[J].中国药房,2017,28(28):4003-4007.
18 孟登科,周静,楼建秀,等.清宫汤抗焦虑作用实验研究[J].浙江中西医结合杂志2014,24(1):18-20.
19 郭花玲,崔瑛,李阳阳,等.黄连对小鼠焦虑行为影响的研究[J].中国实验方剂学杂志,2011,17(15):160-172.
20 刘挺.百合地黄汤的临床应用[J].内蒙古中医药,2014,22(5):94-96.
21 梁来德.百合地黄汤新用[J].现代中西药结合杂志,2010,19(4):481.
22 Li RZ,Guo JY,Li CY,et al.Progress of the research in GABAreceptors and anxiety disorders: Mechanism and pharmaceuticalintervertien[J]. Chin Pharmacoll Bull,2010,26(9) : 989-994.
23 Huang MP,Radadia K,Macone BW, et al.Effects of eszopiclone and zolpidem on sleep-wake behavior, anxiety-like behavior and contextual memory in rats[J]. Behavioural Brain Research, 2010, 210(1): 54-66.
24 Sorra K, Chen CS, Chang CF, et al. Synthesis, anticonvulsant, sedative and anxiolytic activities of novel annulated pyrrolo [1,4] benzodiazepines[J]. International Journal of Molecular Sciences, 2014, 15(9): 16500-16510.
25 王春梅,李贺,孙靖辉,等.北五味子多糖抗焦虑和镇静催眠作用[J].食品科学 2015,36(13):239-242.