福建漳州番茄黄化曲叶病病原分子诊断及暴发成因分析
|
摘要
2017年9月福建漳州地区龙海市东园乡番茄(Solanum lycopersicum)种植区暴发病毒病害,导致严重减产,同时病区内烟粉虱(Bemisia tabaci)虫害发生严重。为明确该地区番茄病毒病突然成灾原因,本研究采用分子生物学方法对番茄病毒病原、病区内烟粉虱的生物型以及虫体带毒情况进行分子检测,并通过室内接毒实验对烟粉虱的传毒性和传毒能力进行测定。结果表明,漳州番茄病毒病原为番茄黄化曲叶病毒(Tomato yellow leaf curl virus, TYLCV);该病区采集的烟粉虱为B生物型,B型烟粉虱具有极强的获毒和传毒能力;虫体对TYLCV病毒携带率为100%。本研究证实了外来入侵烟粉虱在TYLCV病毒传播中的重要媒介作用,揭示了福建省番茄种植区病毒暴发成灾的主要原因,这为将来该病毒的防治提供了依据和帮助。
In September of 2017, a serious virus disease of tomato(Solanum lycopersicum) broke out in Dongyuan town, Zhangzhou city, Fujian province, which caused severe yield loss and accompanied with the occurrence of a large number of whiteflies(Bemisia tabaci). In order to identify what type of the pathogenic virus, molecular diagnosis of the virus strain isolates was done in this research, and then, the biotype and the virus carrying rate of B. tabaci were surveyed. At the same time, the acquisition, transmission and retention of gemini viruses by B. tabaci were studied in laboratory. The results revealed that the virus sample was Tomato yellow leaf curl virus(TYLCV), the B. tabaci was B-biotype and had a very strong ability to acquire and transmit the TYLCV. The virus-carrying rate could reach up to 100%. All this indicated that whiteflytransmitted gemini viruses(WTG) was a main reason to cause the outbreak of TYLCV disease on tomato in Zhangzhou city. This will provide the basis and help for the prevention and control of TYLCV in the future.
In September of 2017, a serious virus disease of tomato(Solanum lycopersicum) broke out in Dongyuan town, Zhangzhou city, Fujian province, which caused severe yield loss and accompanied with the occurrence of a large number of whiteflies(Bemisia tabaci). In order to identify what type of the pathogenic virus, molecular diagnosis of the virus strain isolates was done in this research, and then, the biotype and the virus carrying rate of B. tabaci were surveyed. At the same time, the acquisition, transmission and retention of gemini viruses by B. tabaci were studied in laboratory. The results revealed that the virus sample was Tomato yellow leaf curl virus(TYLCV), the B. tabaci was B-biotype and had a very strong ability to acquire and transmit the TYLCV. The virus-carrying rate could reach up to 100%. All this indicated that whiteflytransmitted gemini viruses(WTG) was a main reason to cause the outbreak of TYLCV disease on tomato in Zhangzhou city. This will provide the basis and help for the prevention and control of TYLCV in the future.
引文
曹骞,李晶,买热木古丽.克依木.等.2013.新疆地区烟粉虱生物型的区域分布及其携带的番茄黄化曲叶病毒检测[J].昆虫学报,56(6):652-664.(Cao Q,Li J,Mair Emuguli·Keyimu,et al.2013.Biotype distribution and infection status of Tomato yellow leaf curl virus(TYL-CV)in Bemisia tabaci(Hemiptera:Aleyrodidae)in Xinjiang,northwestern China[J].Acta Entomologica Sinica,56(6):652-664.)
纠敏,周雪平,刘树生.2006.烟粉虱传播双生病毒研究进展[J].昆虫学报,49(3):513-520.(Jiu M,Zhou X P,Liu SS,2006,Transmission of geminiviruses by whitefly[J].Acta Entomologica Sinica,49(3):513-520.)
李刚,李现道,赵黎明,等.2013.山东烟草番茄黄化曲叶病毒的分子检测和序列分析[J].中国烟草科学,34(6):98-102.(Li G,Li X D,Zhao L M,et al.2013,Molecular detection and sequence analysis of Tomato yellow leaf curl virus infecting tobacco in Shandong Province[J].Chinese Tobacco Science,34(6):98-102.)
潘慧鹏,戈大庆,王少丽,等.2010.在北京和河北局部地区Q型烟粉虱取代B型烟粉虱[J].植物保护,36(6):40-44.(Pan H P,Ge D Q,Wang S L,et al.2010;Replacement of B biotype Bemisia tabaci by Q biotype B.tabaci in some areas of Beijing and Hebei[J].Plant Protection,36(6):40-44.)
汤亚飞,何自福,杜振国,等.2015.番茄黄化曲叶病毒在湖北首次报道[J].植物保护,41(5):233-236.(Tang Y F,He Z F,Du Z G,et al.2015,First report of Tomato yellow leaf curl virus in Hubei province of China[J].Plant Protection,41(5):233-236.)
田鹏,艾鹏飞,宋建军,等.2013.番茄粉虱传双生病毒在中国的传播、分布及分子进化研究进展[J].中国农学通报,29(1):60-64.(Tian P,Ai P F,Song J J,et al.2013The Progress of sequence analysis,spread and distribution of White fly-transmitted geminivirus in tomato in China[J].Chinese Agricultural Science Bulletin,29(1)60-64.)
王昱超,穆常青,郭晓军,等.2013.番茄黄化曲叶病毒对烟粉虱MED隐种取食行为的影响[J].环境昆虫学报,35(4):415-421.(Wan L C,Mu C Q,Guo X J,et al.2013Probing and feeding behaviors of the MED cryptic species of Bemisia tabaci(Gennadius)effected by TYLCV[J].Journal of Evironmental Entomology,35(4):415-421.)
吴淑华,李廷芳,赵文浩,等.2016.江苏省番茄黄化曲叶病毒和褪绿病毒复合侵染的分子检测[J].园艺学报,43(1):89-99.(Wu S H,Li T F,Zhao W H,et al.2016,Molecular identification on mixed infection of Tomato yellow leaf curl virus and tomato chlorosis virus on tomato in Jiangsu Province[J].Act Horticulturae Sinica,43(1)89-99.)
张洁,林文武,宛柏杰,等.2016.福州番茄黄化曲叶病毒的全基因组结构特征[J].福建农林大学学报,45(5):501-504.(Zhang J,Lin W W,Wan B J,et al.2016,Molecular characterization of Tomato yellow leaf curl virus in Fuzhou[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),45(5):501-504.)
郑宇,姚凤銮,黄晓燕,等.2016.福建省烟粉虱不同地理种群遗传结构特征[J].植物保护学报,43(1):78-83(Zheng Y,Yao F L,Huang X Y,et al.2016,Genetic structure of the geographical populations of tobacco whitefly Bemisia tabaci(Gennadius)in Fujian,China[J].Acta Phytophylacica Sinica,43(1):78-83.)
郑宇,赵建伟,何玉仙,等.2012.福建省烟粉虱田间种群抗药性发展及其影响因素[J].应用生态学报,23(1):271-277.(Zheng Y,Zhao J W,He Y X,et al.2012,Development of insecticide resistance and its effect factors in field population of Bemisia tabaci in Fujian province east China[J].Chinese Journal of Applied Ecology23(1):271-277)
Colvin J,Omongo C A,Govindappa M R,et al.2006.Hostplant viral infection effects on arthropodvector population growth,development and behaviour:Managemen and epidemiological implications[J].Advances in Virus Research,67(6):419-452.
De Barro P J,Liu S S,Boykin L M,et al.2011.Bemisia tabaci:A statement of species status[J].Annual Review of Entomology.56(1):1-19.
Fauquet C M,Bisaro D M,Briddon R W,et al.2003.Revision of taxonomic criteria for species demarcation in the family Geminiviridae,and an updated list of begomovirus species[J].Archives of Virology,148(2):405-421.
Fauquet C M,Briddon R W,Brown J K,et al.2008.Geminivirus strain demarcation and nomenclature[J].Archives of Virology,153(4):783-821.
Harrison B,Robinson D.1999.Natural genomic and antigenic variation in whitefly-transmitted geminiviruses(Begomo viruses)[J].Annual Review of Phytopathol,37(4):369-398.
Pan H P,Chu D,Ge D Q,et al.2011.Further spread of and domination by Bemisia tabaci(Hemiptera:Aleyrodidae)biotype Q on field crops in China[J].Journal of Economic Entomology,104(3):978-985.
Pan H P,Chu D,Yan W Q,et al.2012.Rapid spread of tomato yellow leaf curl virus in China is aided differentially by two invasive whiteflies[J].PloS One,7(4):e34817.
Polston J E,Anderson P K.1997.The emergence of whiteflytransmitted geminiviruses in tomato in the western hemisphere[J].Plant Disease,81(12):1358-1369.
Rekha A R,Maruthi MNV,Munivappa V.2005;Occurrence of three genotypic clusters of Bemisia tabaci and the rapid spread of the B biotype in south India[J].Entomologia Experimentalis et Applicata,117(3):221-233.
Shatters R G,Powell C A,Boykin LM,et al.2009.Improved DNA barcoding method for Bemisia tabaci and related Aleyrodidae:Development of universal and Bemisia tabaci biotype-specific mitochondrial cytochrome c oxidaseI polymerase chain reaction primers[J].Journal of Economic Entomology,102(2):750-758.
Wu J B,Dai F M,Zhou X P.2006.First report of Tomato yellow leaf curl virus in China[J].Plant Disease,90(10):1359-1359.
Yao F L,Zheng Y,Huang XY,et al.2017.Dynamics of Bemisia tabaci biotypes and insecticide resistance in Fujian province in China during 2005-2014[J].Scientific Reports,7:40803.doi:10.1038/srep40803
纠敏,周雪平,刘树生.2006.烟粉虱传播双生病毒研究进展[J].昆虫学报,49(3):513-520.(Jiu M,Zhou X P,Liu SS,2006,Transmission of geminiviruses by whitefly[J].Acta Entomologica Sinica,49(3):513-520.)
李刚,李现道,赵黎明,等.2013.山东烟草番茄黄化曲叶病毒的分子检测和序列分析[J].中国烟草科学,34(6):98-102.(Li G,Li X D,Zhao L M,et al.2013,Molecular detection and sequence analysis of Tomato yellow leaf curl virus infecting tobacco in Shandong Province[J].Chinese Tobacco Science,34(6):98-102.)
潘慧鹏,戈大庆,王少丽,等.2010.在北京和河北局部地区Q型烟粉虱取代B型烟粉虱[J].植物保护,36(6):40-44.(Pan H P,Ge D Q,Wang S L,et al.2010;Replacement of B biotype Bemisia tabaci by Q biotype B.tabaci in some areas of Beijing and Hebei[J].Plant Protection,36(6):40-44.)
汤亚飞,何自福,杜振国,等.2015.番茄黄化曲叶病毒在湖北首次报道[J].植物保护,41(5):233-236.(Tang Y F,He Z F,Du Z G,et al.2015,First report of Tomato yellow leaf curl virus in Hubei province of China[J].Plant Protection,41(5):233-236.)
田鹏,艾鹏飞,宋建军,等.2013.番茄粉虱传双生病毒在中国的传播、分布及分子进化研究进展[J].中国农学通报,29(1):60-64.(Tian P,Ai P F,Song J J,et al.2013The Progress of sequence analysis,spread and distribution of White fly-transmitted geminivirus in tomato in China[J].Chinese Agricultural Science Bulletin,29(1)60-64.)
王昱超,穆常青,郭晓军,等.2013.番茄黄化曲叶病毒对烟粉虱MED隐种取食行为的影响[J].环境昆虫学报,35(4):415-421.(Wan L C,Mu C Q,Guo X J,et al.2013Probing and feeding behaviors of the MED cryptic species of Bemisia tabaci(Gennadius)effected by TYLCV[J].Journal of Evironmental Entomology,35(4):415-421.)
吴淑华,李廷芳,赵文浩,等.2016.江苏省番茄黄化曲叶病毒和褪绿病毒复合侵染的分子检测[J].园艺学报,43(1):89-99.(Wu S H,Li T F,Zhao W H,et al.2016,Molecular identification on mixed infection of Tomato yellow leaf curl virus and tomato chlorosis virus on tomato in Jiangsu Province[J].Act Horticulturae Sinica,43(1)89-99.)
张洁,林文武,宛柏杰,等.2016.福州番茄黄化曲叶病毒的全基因组结构特征[J].福建农林大学学报,45(5):501-504.(Zhang J,Lin W W,Wan B J,et al.2016,Molecular characterization of Tomato yellow leaf curl virus in Fuzhou[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),45(5):501-504.)
郑宇,姚凤銮,黄晓燕,等.2016.福建省烟粉虱不同地理种群遗传结构特征[J].植物保护学报,43(1):78-83(Zheng Y,Yao F L,Huang X Y,et al.2016,Genetic structure of the geographical populations of tobacco whitefly Bemisia tabaci(Gennadius)in Fujian,China[J].Acta Phytophylacica Sinica,43(1):78-83.)
郑宇,赵建伟,何玉仙,等.2012.福建省烟粉虱田间种群抗药性发展及其影响因素[J].应用生态学报,23(1):271-277.(Zheng Y,Zhao J W,He Y X,et al.2012,Development of insecticide resistance and its effect factors in field population of Bemisia tabaci in Fujian province east China[J].Chinese Journal of Applied Ecology23(1):271-277)
Colvin J,Omongo C A,Govindappa M R,et al.2006.Hostplant viral infection effects on arthropodvector population growth,development and behaviour:Managemen and epidemiological implications[J].Advances in Virus Research,67(6):419-452.
De Barro P J,Liu S S,Boykin L M,et al.2011.Bemisia tabaci:A statement of species status[J].Annual Review of Entomology.56(1):1-19.
Fauquet C M,Bisaro D M,Briddon R W,et al.2003.Revision of taxonomic criteria for species demarcation in the family Geminiviridae,and an updated list of begomovirus species[J].Archives of Virology,148(2):405-421.
Fauquet C M,Briddon R W,Brown J K,et al.2008.Geminivirus strain demarcation and nomenclature[J].Archives of Virology,153(4):783-821.
Harrison B,Robinson D.1999.Natural genomic and antigenic variation in whitefly-transmitted geminiviruses(Begomo viruses)[J].Annual Review of Phytopathol,37(4):369-398.
Pan H P,Chu D,Ge D Q,et al.2011.Further spread of and domination by Bemisia tabaci(Hemiptera:Aleyrodidae)biotype Q on field crops in China[J].Journal of Economic Entomology,104(3):978-985.
Pan H P,Chu D,Yan W Q,et al.2012.Rapid spread of tomato yellow leaf curl virus in China is aided differentially by two invasive whiteflies[J].PloS One,7(4):e34817.
Polston J E,Anderson P K.1997.The emergence of whiteflytransmitted geminiviruses in tomato in the western hemisphere[J].Plant Disease,81(12):1358-1369.
Rekha A R,Maruthi MNV,Munivappa V.2005;Occurrence of three genotypic clusters of Bemisia tabaci and the rapid spread of the B biotype in south India[J].Entomologia Experimentalis et Applicata,117(3):221-233.
Shatters R G,Powell C A,Boykin LM,et al.2009.Improved DNA barcoding method for Bemisia tabaci and related Aleyrodidae:Development of universal and Bemisia tabaci biotype-specific mitochondrial cytochrome c oxidaseI polymerase chain reaction primers[J].Journal of Economic Entomology,102(2):750-758.
Wu J B,Dai F M,Zhou X P.2006.First report of Tomato yellow leaf curl virus in China[J].Plant Disease,90(10):1359-1359.
Yao F L,Zheng Y,Huang XY,et al.2017.Dynamics of Bemisia tabaci biotypes and insecticide resistance in Fujian province in China during 2005-2014[J].Scientific Reports,7:40803.doi:10.1038/srep40803
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |