博落回茎叶血根碱提取工艺的比较研究
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摘要
为博落回提取血根碱开发新原料,为工业化生产提供依据。在单因素试验的基础上,以血根碱转移率为评价指标,分别对博落回茎叶使用酸性乙醇回流和酸水渗漉法进行工艺研究。试验结果表明酸性乙醇回流提取工艺最佳条件为乙醇体积分数90%,液固比301 ml/g,硫酸浓度0.12%,提取温度80℃,提取时间2 h,血根碱转移率达到(89.36±0.49)%(n=5, R~2=0.54%),最终得到博落回茎叶提取物收率(24.27±0.92)%(n=5,R~2=3.77%),血根碱含量(1.65±0.06)%(n=5,R~2=3.36%);酸水渗漉提取工艺最佳条件为硫酸浓度1%,浸泡时间30 min,渗漉温度80℃,溶剂用量30倍,渗漉速度0.5 ml/min,血根碱转移率达到(83.63±2.09)%(n=5,R~2=2.49%),最终得到博落回茎叶提取物收率(21.64±0.78)%(n=5,R~2=3.61%),血根碱含量(2.25±0.11)%(n=5,R~2=4.88%)。两种提取方法中酸水渗漉法碱沉步骤简便,得到的提取物中血根碱的含量比酸性乙醇回流法高出36.36%,溶剂用量一致,在工业化生产中应结合实际情况进一步优化参数条件,为博落回茎叶开发利用提供了理论依据。
For developing new materials and offer a sound evidence for the mass production industry, the extraction process of sanguinarinefrom the stem and leaf of Macleaya cordata wasstudied in this work. On the basis of single factor experiment, the factors affecting the transferrate of sanguinarine investigated by ethanol reflux extraction and percolation extraction. The experiment results indicated that the optimum conditions for the ethanol reflux extraction were obtained as follows:the ethanol concentration was 90%, liquid-solid ratio was 30:1, sulfuric acid concentration was 0.12%, extraction temperature was 80 ℃and extraction time was 2 h. Under the conditions, the transfer rate of sanguinarine was(89.36±0.49)%(n=5, R~2=0.54%), the content of sanguinarine in the extract obtained from the final is(1.65±0.06)%(n=5, R~2=3.36%), the yield was(24.27±0.92)%(n=5, R~2=3.77%), and the percolation extraction as follows,sulfuric acid concentration was 1%, soaking time was 30 min, percolation temperature was 80 ℃, liquid-solid ratio was 30:1 ml/g and percolation rate was 0.5 ml/min. Under the conditions, the transferrate of sanguinarine was(83.63±2.09)%(n=5, R~2=2.49%), the content of sanguinarine in the final extract was(2.25±0.11)%(n=5, R~2=4.88%), the yield was(21.64±0.78)%(n=5, R~2=3.61%). In the two extraction methods, the acid water percolation method has a simple alkali precipitation step than acidethanol reflux extraction. The content of sanguinarine in the extracts was 36.36% higher than acid etha-nol reflux method, and the solvent dosage is consistent. In the industrial production, the parametersshould be controlled according to the actual situation. The research can provide data for the stem andleaf of Macleaya cordata's development and utilization.
For developing new materials and offer a sound evidence for the mass production industry, the extraction process of sanguinarinefrom the stem and leaf of Macleaya cordata wasstudied in this work. On the basis of single factor experiment, the factors affecting the transferrate of sanguinarine investigated by ethanol reflux extraction and percolation extraction. The experiment results indicated that the optimum conditions for the ethanol reflux extraction were obtained as follows:the ethanol concentration was 90%, liquid-solid ratio was 30:1, sulfuric acid concentration was 0.12%, extraction temperature was 80 ℃and extraction time was 2 h. Under the conditions, the transfer rate of sanguinarine was(89.36±0.49)%(n=5, R~2=0.54%), the content of sanguinarine in the extract obtained from the final is(1.65±0.06)%(n=5, R~2=3.36%), the yield was(24.27±0.92)%(n=5, R~2=3.77%), and the percolation extraction as follows,sulfuric acid concentration was 1%, soaking time was 30 min, percolation temperature was 80 ℃, liquid-solid ratio was 30:1 ml/g and percolation rate was 0.5 ml/min. Under the conditions, the transferrate of sanguinarine was(83.63±2.09)%(n=5, R~2=2.49%), the content of sanguinarine in the final extract was(2.25±0.11)%(n=5, R~2=4.88%), the yield was(21.64±0.78)%(n=5, R~2=3.61%). In the two extraction methods, the acid water percolation method has a simple alkali precipitation step than acidethanol reflux extraction. The content of sanguinarine in the extracts was 36.36% higher than acid etha-nol reflux method, and the solvent dosage is consistent. In the industrial production, the parametersshould be controlled according to the actual situation. The research can provide data for the stem andleaf of Macleaya cordata's development and utilization.
引文
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[7]邓光辉,罗珍连.毛细管电泳-电化学发光同时测定博落回果实中血根碱和白屈菜红碱的含量[J].分子科学学报,2018,34(2):134-138.
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[2]赵静.滥用兽用抗生素的危害[J].当代畜禽养殖业,2017(6):53.
[3]徐海燕,汪孟娟,王京京,等.博落回对小鼠肠道菌群及血液生化指标的影响[J].饲料博览,2014(1):41-44.
[4]侯博,曾建国.血根碱的生物学活性及博落回提取物在动物生产中的应用[J].动物营养学报,2018,30(2):413-420.
[5]贺云海,刘婷婷.美佑壮:后抗生素时代的引跑者[J].新闻天地(上半月),2012(1):74-75.
[6]曾建国.博落回资源为示范的中药材全产业链研究与综合利用[J].中国现代中药,2017,19(10):1359-1366.
[7]邓光辉,罗珍连.毛细管电泳-电化学发光同时测定博落回果实中血根碱和白屈菜红碱的含量[J].分子科学学报,2018,34(2):134-138.
[8]中华人民共和国农业部公告第1597号[M].北京:中华人民共和国农业部,2011:11.
[9]曾建国,刘华.博落回主要功能成分及其在动物生产中的应用[J].饲料工业,2019,40(7):1-6.
[10]曾建国,罗炼辉,谈满良.一种博落回提取物的制备方法,CN101849994A[P].湖南:CN101849994A.2010-10-06.