甘蔗与斑茅割手密复合体(GXAS07-6-1)杂交后代的染色体遗传分析
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摘要
本研究的目的是明确甘蔗与斑割(斑茅割手密)复合体杂交后代F_1材料的染色体核型构成和遗传行为,为有效利用甘蔗野生种质提供细胞遗传学的理论依据。采用甘蔗根尖细胞酶解去壁低渗法分别对3个不同甘蔗品种(系)与斑割复合体GXAS07-6-1的杂交后代进行染色体数目鉴定和核型分析。结果表明:各亲本和后代材料染色体数目在不同细胞中不恒定,染色体变幅为6~11条。粤糖93-159与GXAS07-6-1的F1材料GXASF108-1-1染色体核型公式为2n=94=91 m+3 sm;桂糖01-53与GXAS07-6-1的F_1材料GXASF108-2-28染色体核型公式为2n=98=88 m+10 sm(1SAT);桂糖02-761与GXAS07-6-1的F_1材料GXASF108-3-7染色体核型公式为2n=92=87m+5 sm;3个F1材料的核型均为2B型。推断甘蔗与斑割复合体杂交亲子间的染色体基本按"n+n"方式传递,同时可能存在部分染色体加倍现象,它们的杂种F_1核型均为较原始的染色体2B类型。
To incorporat e genes from wild germplasm for laying a good cytogenetical foundation of sugarcane,karyotype composition and genetic behavior of chromosome were explored for the F_1 progeny between sugarcane( Saccharum spp.)and intergeneric hybrid(Erianthus arundinaceus ×Saccharum spontaneum). Chromosome number calculation and karyotype analysis were carried out for the progeny between 3 clones(Saccharum spp.)and GXAS07-6-1(Erianthus arundinaceus ×Saccharum spontaneum)by the means of cell wall degradation hypotonic smear method. The somatic chromosome numbers of all accessions were non-constant and the amplitude was 6-11.The somatic chromosome karyotypic types of F_1 progeny for 3 crosses were 2n=94=91 m+3 sm, 2n=98=88 m+10 sm(1SAT), and 2n=92=87 m+5 sm, respectively. The "n+n" transmission and 2B karyotype of F_1 progeny from sugarcane and intergeneric hybrid were indicated. The chromosomes of sugarcane and intergeneric hybrid complex(Erianthus arundinaceus ×Saccharum spontaneum)were transmitted in the mode " n +n" but doubling possibly existed. The karyotypes of these three F_1 progenies belong to the primitive 2B type.
To incorporat e genes from wild germplasm for laying a good cytogenetical foundation of sugarcane,karyotype composition and genetic behavior of chromosome were explored for the F_1 progeny between sugarcane( Saccharum spp.)and intergeneric hybrid(Erianthus arundinaceus ×Saccharum spontaneum). Chromosome number calculation and karyotype analysis were carried out for the progeny between 3 clones(Saccharum spp.)and GXAS07-6-1(Erianthus arundinaceus ×Saccharum spontaneum)by the means of cell wall degradation hypotonic smear method. The somatic chromosome numbers of all accessions were non-constant and the amplitude was 6-11.The somatic chromosome karyotypic types of F_1 progeny for 3 crosses were 2n=94=91 m+3 sm, 2n=98=88 m+10 sm(1SAT), and 2n=92=87 m+5 sm, respectively. The "n+n" transmission and 2B karyotype of F_1 progeny from sugarcane and intergeneric hybrid were indicated. The chromosomes of sugarcane and intergeneric hybrid complex(Erianthus arundinaceus ×Saccharum spontaneum)were transmitted in the mode " n +n" but doubling possibly existed. The karyotypes of these three F_1 progenies belong to the primitive 2B type.
引文
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[2]Irvine J E.Saccharum species as horticultural classes[J].Theoretical and Applied Genetics,1999,98(2):186-194.
[3]张革民,刘昔辉,方锋学,等.斑茅割手密复合体创制及其与甘蔗杂交F1的染色体计数和形态表现[C]//2009年中国作物学会学术年会论文摘要集.2009.
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[7]王先宏,杨清辉,李富生,等.基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析[J].中国农业科学,2011,44(6):1 085-1 091.
[8]刘昔辉,方锋学,高轶静,等.斑茅割手密杂种后代真实性鉴定及遗传分析[J].作物学报,2012,38(5):914-920
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[10]刘许辉.甘蔗与斑茅割手密复合体杂交后代的遗传分析[D].南宁:广西大学,2013.
[11]黄东益,郑成木,庄南生,等.甘蔗染色体组构成系统演化的研究[J].热带作物学报,2000,21(1):43-51.
[12]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-302.
[13]刘少谋,符成,陈勇生.近十年海南甘蔗育种场斑茅后代回交利用研究[J].甘蔗糖业,2007(2):1-6.
[14]王丽萍,蔡青,范源洪,等.甘蔗(Saccharum)与斑茅(Erianthus arundtnaceus)远缘杂交利用研究[J].西南农业学报,2007,20(4):721-726.
[15]吴嘉云.甘蔗与斑茅后代染色体遗传分析及抗性初步评价[D].福州:福建农林大学,2013.
[16]王先宏,李富生,何丽莲,等.甘蔗与蔗茅杂交F2BC1代的染色体遗传分析[J].中国农学通报,2014,30(15):81-85.
[17]Bremer G.Problems in breeding and cytology of sugarcane[J].Euphytica,1961,10(1):59-78
[18]刘文荣,邓祖湖,张木清,等.甘蔗斑茅的杂交利用及其杂种后代鉴定系列研究Ⅲ.甘蔗斑茅远缘杂交后代细胞遗传分析[J].作物学报,2004,30(11):1 093-1 096.
[19]黄永吉,吴嘉云,刘少谋,等.基于GISH的甘蔗与斑茅F1染色体遗传与核型分析[J].植物资源遗传学报,2014,15(2):394-398.
[20]Stebbins G L.Chromosomal evolution in higher plants[M].London:Edward Arnold,1971:87-89.
[2]Irvine J E.Saccharum species as horticultural classes[J].Theoretical and Applied Genetics,1999,98(2):186-194.
[3]张革民,刘昔辉,方锋学,等.斑茅割手密复合体创制及其与甘蔗杂交F1的染色体计数和形态表现[C]//2009年中国作物学会学术年会论文摘要集.2009.
[4]郑成木.甘蔗核型及其染色体数目变化的研究[J].热带作物学报,1993,14(2):47-51.
[5]Bremer G.A cytological investigation of some cultivated kinds and of their parents[J].Genetica,1924,6:497-525.
[6]邓祖湖,李玉蝉,刘文荣,等.甘蔗和斑茅远缘杂交后代的染色体遗传分析[J].热带作物学报,2007,28(3):62-67.
[7]王先宏,杨清辉,李富生,等.基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析[J].中国农业科学,2011,44(6):1 085-1 091.
[8]刘昔辉,方锋学,高轶静,等.斑茅割手密杂种后代真实性鉴定及遗传分析[J].作物学报,2012,38(5):914-920
[9]高轶静,方锋学,刘昔辉,等.甘蔗与斑茅割手密复合体杂交后代的分子标记鉴定[J].植物遗传资源学报,2012,13(5):912-916.
[10]刘许辉.甘蔗与斑茅割手密复合体杂交后代的遗传分析[D].南宁:广西大学,2013.
[11]黄东益,郑成木,庄南生,等.甘蔗染色体组构成系统演化的研究[J].热带作物学报,2000,21(1):43-51.
[12]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-302.
[13]刘少谋,符成,陈勇生.近十年海南甘蔗育种场斑茅后代回交利用研究[J].甘蔗糖业,2007(2):1-6.
[14]王丽萍,蔡青,范源洪,等.甘蔗(Saccharum)与斑茅(Erianthus arundtnaceus)远缘杂交利用研究[J].西南农业学报,2007,20(4):721-726.
[15]吴嘉云.甘蔗与斑茅后代染色体遗传分析及抗性初步评价[D].福州:福建农林大学,2013.
[16]王先宏,李富生,何丽莲,等.甘蔗与蔗茅杂交F2BC1代的染色体遗传分析[J].中国农学通报,2014,30(15):81-85.
[17]Bremer G.Problems in breeding and cytology of sugarcane[J].Euphytica,1961,10(1):59-78
[18]刘文荣,邓祖湖,张木清,等.甘蔗斑茅的杂交利用及其杂种后代鉴定系列研究Ⅲ.甘蔗斑茅远缘杂交后代细胞遗传分析[J].作物学报,2004,30(11):1 093-1 096.
[19]黄永吉,吴嘉云,刘少谋,等.基于GISH的甘蔗与斑茅F1染色体遗传与核型分析[J].植物资源遗传学报,2014,15(2):394-398.
[20]Stebbins G L.Chromosomal evolution in higher plants[M].London:Edward Arnold,1971:87-89.