水稻响应重金属胁迫的蛋白质组学研究进展
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摘要
水稻(Oryza sativa L.)是全球主要粮食作物之一,其重金属污染问题值得关注。综述了重金属胁迫对水稻造成毒害时所引起的蛋白质组学水平的变化情况。在现有的国内外研究进展中可发现,水稻受到镉、铜、砷、铝胁迫时,与氧化应激反应相关蛋白的表达会受到明显响应。当前,蛋白质组学的研究主要集中于水稻响应镉胁迫,对于其他重金属胁迫的响应蛋白研究相对较少。现有研究可知,镉和砷的响应蛋白类型较丰富,功能也较相似;水稻在响应铜胁迫时可能通过降低代谢,增强防御机制来响应胁迫伤害;水稻响应汞胁迫时的响应蛋白,主要是与光合作用与抗氧化过程相关蛋白。
Rice is the staple food.Heavy metals accumulation in rice has been paid more attention.It was reviewed the changes of protein of rice plants was affected by different kinds of heavy metals.Based on the previous studies,the expression of protein that related to oxidative stress would be changed when plants were exposed by Cd,Cu,As and Al.Currently,the study of proteomics was mainly focused on rice exposed by Cd,and there was relatively little research on the response protein of other heavy metal stress.It was showed that the types of response protein were abundant and the function was similar when it was exposed by Cd and As;rice could resist coercion by lowering metabolism and strengthening defense mechanism in response to Cu stress;the response protein of rice exposed by Hg was mainly related to photosynthesis and antioxidation process.
Rice is the staple food.Heavy metals accumulation in rice has been paid more attention.It was reviewed the changes of protein of rice plants was affected by different kinds of heavy metals.Based on the previous studies,the expression of protein that related to oxidative stress would be changed when plants were exposed by Cd,Cu,As and Al.Currently,the study of proteomics was mainly focused on rice exposed by Cd,and there was relatively little research on the response protein of other heavy metal stress.It was showed that the types of response protein were abundant and the function was similar when it was exposed by Cd and As;rice could resist coercion by lowering metabolism and strengthening defense mechanism in response to Cu stress;the response protein of rice exposed by Hg was mainly related to photosynthesis and antioxidation process.
引文
[1]王斌,姚勤,陈克平.水稻响应非生物逆境胁迫的蛋白质组学研究进展[J].安徽农业科学,2012,40(12):6989-6992.
[2]GILLER K E,WITTER E,MCGRATH S P.Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils:a review[J].Soil Biology and Biochemistry,1998,30(10/11).
[3]郭锦,周启星.污染胁迫下的蛋白质组学研究[J].世界科技研究与发展,2014,36(2):191-199.
[4]孔祥生,张妙霞,郭秀璞.Cd毒害对玉米幼苗细胞膜通透性及保护酶活性的影响[J].农业环境保护,1999,18(3):133-134.
[5]郭庶,彭晓武,刘芸,等.蛋白质组学技术在生态毒理学研究中的应用[J].环境卫生学杂志,2009(2):89-92.
[6]BARI R,JONES J D.Role of plant hormones in plant defence responses[J].Plant Molecular Biology,2009,69(4):473-488.
[7]杨扬,王小燕,王江,等.物种多样性对植物生长与土壤镉污染修复的影响[J].环境科学学报,2006,36(6):2103-2113.
[8]HAJDUCH M,RAKWAL R,AGRAWAL G K,et al.Highresolution two-dimensional electrophoresis separation of proteins from metal-stressed rice(Oryza sativa L.)leaves:drastic reductions/fragmentation of ribulose-1,5-bisphosphate carboxylase/oxygenase and induction of stress-related proteins[J].Electrophoresis,2001,22(13).
[9]LEE K,BAE D W,KIM S H,et al.Comparative proteomic analysis of the short-term responses of rice roots and leaves to cadmium[J].Journal of Plant Physiology,2010,167(3).
[10]肖清铁,戎红,周丽英,等.水稻叶片对镉胁迫响应的蛋白质差异表达[J].应用生态学报,2011,22(4):1013-1019.
[11]LIN S,WAN W,TIAN T,et al.Protein complex and proteomic profile in the roots of Oryza sativa L.in response to cadmium toxicity[J].Acta Physiologiae Plantarum,2015,37(9):188.
[12]AINA R,LABRA M,FUMAGALLI P,et al.Thiol-peptide level and proteomic changes in response to cadmium toxicity in Oryza sativa L.roots[J].Environmental and Experimental Botany,2007,59(3):381-392.
[13]KIM Y K,LEE M Y.Proteomic analysis of differentially expressed proteins of rice in response to cadmium[J].Journal of the Korean Society for Applied Biological Chemistry,2009,52(5):428-436.
[14]AHSAN N,LEE S H,LEE D G,et al.Physiological and protein profiles alternation of germinating rice seedlings exposed to acute cadmium toxicity[J].Comptes Rendus Biologies,2007,330(10):735-746.
[15]彭舒,黄真池,欧阳乐军,等.铜、镉、汞对水稻幼苗抗氧化酶活性的影响[J].湛江师范学院学报,2012,33(3):78-85.
[16]AHSAN N,LEE D G,LEE S H,et al.Excess copper induced physiological and proteomic changes in germinating rice seeds[J].Chemosphere,2007,67(6).
[17]AHSAN N,LEE D G,ALAM I,et al.Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress[J].Proteomics,2008,8(17).
[18]REQUEJO R,TENA M.Maize response to acute arsenic toxicity as revealed by proteome analysis of plant shoots[J].Proteomics,2006,6(S1):156-162.
[19]FUKUDA T,SAITO A,WASAKI J,et al.Metabolic alterations proposed by proteome in rice roots grown under low P and high Al concentration under low pH[J].Plant Science,2007,172(6):1157-1165.
[20]YANG Q,WANG Y,ZHANG J,et al.Identification of aluminum-responsive proteins in rice roots by a proteomic approach:cysteine synthase as a key player in Al response[J].Proteomics,2007,7(5):737-749.
[21]CHEN Y A,CHI W C,HUANG T L,et al.Mercury-induced biochemical and proteomic changes in rice roots[J].Plant Physiology&Biochemistry,2012,55(2).
[22]郭美丽,高维锡,高会东,等.植物响应环境胁迫过程中的蛋白质组变化规律研究进展[J].广东农业科学,2011,38(20):126-129.
[23]金勇,付庆灵,郑进.超积累植物修复铜污染土壤的研究现状[J].中国农业科技导报,2012,14(4):93-100.
[24]李圣发.土壤砷污染及其植物修复的研究进展与展望[J].地球与环境,2011,39(3):429-434.
[25]AHSAN N,LEE D G,KIM K H,et al.Analysis of arsenic stress-induced differentially expressed proteins in rice leaves by two-dimensional gel electrophoresis coupled with mass spectrometry[J].Chemosphere,2010,78(3):224-231.
[26]汤华,向福英,柳晓磊,等.植物铝离子胁迫的研究方法综述[J].武汉植物学研究,2007,25(5):494-499.
[27]ZHOU S,SAUVE R,THANNHAUSER T W.Proteome changes induced by aluminium stress in tomato roots[J].Journal of Experimental Botany,2009,60(6):1849-1857.
[28]DU X,ZHU Y G,LIU W J,et al.Uptake of mercury(Hg)by seedlings of rice(Oryza sativa L.)grown in solution culture and interactions with arsenate uptake[J].Environmental and Experimental Botany,2005,54(1):1-7.
[29]CARGNELUTTI D,TABALDI L A,SPANEVELLO R M,et al.Mercury toxicity induces oxidative stress in growing cucumber seedlings[J].Chemosphere,2006,65(6):999-1006.
[2]GILLER K E,WITTER E,MCGRATH S P.Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils:a review[J].Soil Biology and Biochemistry,1998,30(10/11).
[3]郭锦,周启星.污染胁迫下的蛋白质组学研究[J].世界科技研究与发展,2014,36(2):191-199.
[4]孔祥生,张妙霞,郭秀璞.Cd毒害对玉米幼苗细胞膜通透性及保护酶活性的影响[J].农业环境保护,1999,18(3):133-134.
[5]郭庶,彭晓武,刘芸,等.蛋白质组学技术在生态毒理学研究中的应用[J].环境卫生学杂志,2009(2):89-92.
[6]BARI R,JONES J D.Role of plant hormones in plant defence responses[J].Plant Molecular Biology,2009,69(4):473-488.
[7]杨扬,王小燕,王江,等.物种多样性对植物生长与土壤镉污染修复的影响[J].环境科学学报,2006,36(6):2103-2113.
[8]HAJDUCH M,RAKWAL R,AGRAWAL G K,et al.Highresolution two-dimensional electrophoresis separation of proteins from metal-stressed rice(Oryza sativa L.)leaves:drastic reductions/fragmentation of ribulose-1,5-bisphosphate carboxylase/oxygenase and induction of stress-related proteins[J].Electrophoresis,2001,22(13).
[9]LEE K,BAE D W,KIM S H,et al.Comparative proteomic analysis of the short-term responses of rice roots and leaves to cadmium[J].Journal of Plant Physiology,2010,167(3).
[10]肖清铁,戎红,周丽英,等.水稻叶片对镉胁迫响应的蛋白质差异表达[J].应用生态学报,2011,22(4):1013-1019.
[11]LIN S,WAN W,TIAN T,et al.Protein complex and proteomic profile in the roots of Oryza sativa L.in response to cadmium toxicity[J].Acta Physiologiae Plantarum,2015,37(9):188.
[12]AINA R,LABRA M,FUMAGALLI P,et al.Thiol-peptide level and proteomic changes in response to cadmium toxicity in Oryza sativa L.roots[J].Environmental and Experimental Botany,2007,59(3):381-392.
[13]KIM Y K,LEE M Y.Proteomic analysis of differentially expressed proteins of rice in response to cadmium[J].Journal of the Korean Society for Applied Biological Chemistry,2009,52(5):428-436.
[14]AHSAN N,LEE S H,LEE D G,et al.Physiological and protein profiles alternation of germinating rice seedlings exposed to acute cadmium toxicity[J].Comptes Rendus Biologies,2007,330(10):735-746.
[15]彭舒,黄真池,欧阳乐军,等.铜、镉、汞对水稻幼苗抗氧化酶活性的影响[J].湛江师范学院学报,2012,33(3):78-85.
[16]AHSAN N,LEE D G,LEE S H,et al.Excess copper induced physiological and proteomic changes in germinating rice seeds[J].Chemosphere,2007,67(6).
[17]AHSAN N,LEE D G,ALAM I,et al.Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress[J].Proteomics,2008,8(17).
[18]REQUEJO R,TENA M.Maize response to acute arsenic toxicity as revealed by proteome analysis of plant shoots[J].Proteomics,2006,6(S1):156-162.
[19]FUKUDA T,SAITO A,WASAKI J,et al.Metabolic alterations proposed by proteome in rice roots grown under low P and high Al concentration under low pH[J].Plant Science,2007,172(6):1157-1165.
[20]YANG Q,WANG Y,ZHANG J,et al.Identification of aluminum-responsive proteins in rice roots by a proteomic approach:cysteine synthase as a key player in Al response[J].Proteomics,2007,7(5):737-749.
[21]CHEN Y A,CHI W C,HUANG T L,et al.Mercury-induced biochemical and proteomic changes in rice roots[J].Plant Physiology&Biochemistry,2012,55(2).
[22]郭美丽,高维锡,高会东,等.植物响应环境胁迫过程中的蛋白质组变化规律研究进展[J].广东农业科学,2011,38(20):126-129.
[23]金勇,付庆灵,郑进.超积累植物修复铜污染土壤的研究现状[J].中国农业科技导报,2012,14(4):93-100.
[24]李圣发.土壤砷污染及其植物修复的研究进展与展望[J].地球与环境,2011,39(3):429-434.
[25]AHSAN N,LEE D G,KIM K H,et al.Analysis of arsenic stress-induced differentially expressed proteins in rice leaves by two-dimensional gel electrophoresis coupled with mass spectrometry[J].Chemosphere,2010,78(3):224-231.
[26]汤华,向福英,柳晓磊,等.植物铝离子胁迫的研究方法综述[J].武汉植物学研究,2007,25(5):494-499.
[27]ZHOU S,SAUVE R,THANNHAUSER T W.Proteome changes induced by aluminium stress in tomato roots[J].Journal of Experimental Botany,2009,60(6):1849-1857.
[28]DU X,ZHU Y G,LIU W J,et al.Uptake of mercury(Hg)by seedlings of rice(Oryza sativa L.)grown in solution culture and interactions with arsenate uptake[J].Environmental and Experimental Botany,2005,54(1):1-7.
[29]CARGNELUTTI D,TABALDI L A,SPANEVELLO R M,et al.Mercury toxicity induces oxidative stress in growing cucumber seedlings[J].Chemosphere,2006,65(6):999-1006.