山茶属种质资源亲缘关系的SSR分析
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摘要
为了提高大树油茶嫁接的成活率,为确定最合适的接穗提供科学依据,以不同地域和种源的36份山茶属植物为材料,采用梯度退火温度和正交试验设计的方法,用12对SSR引物进行PCR扩增,并采用UPGMA聚类法对品种间的亲缘关系进行研究。结果表明,12对引物扩增出目的条带,在12个SSR位点共获得214个等位基因。根据各品种12对引物的扩增带型得出,各品种间遗传相似系数范围为0.62～0.95,平均相似系数为0.793。聚类分析结果表明,地域来源近的品种紧密地聚在一起。SSR标记反映出的山茶属种质间的亲缘关系与形态学研究的分类结果并不完全吻合。
In order to improve grafting survival rate of Camellia oleifera big trees and to provide some scientific bases for determining the most suitable scion, 36 Camellia species from different regions and provenances were used as materials, 12 pairs of SSR primers were used for PCR amplification by using gradient annealing temperatures and orthogonal design method, and genetic genetic relationship among clones was researched by using UPGMA clustering method. The results showed that target bands were amplified by 12 pairs of primers, and 214 alleles were obtained at 12 SSR loci. Based on amplified band types of each clone by 12 pairs of primers, genetic similarity coefficients ranged from 0.62 to 0.95 between clones, and average similarity coefficient was 0.793. The results of cluster analysis showed that the clones with closely geographical origins were closely clustered together. SSR markers showed that the phylogenetic relationship among Camellia germplasms was not completely consistent with the classification results of morphology.
In order to improve grafting survival rate of Camellia oleifera big trees and to provide some scientific bases for determining the most suitable scion, 36 Camellia species from different regions and provenances were used as materials, 12 pairs of SSR primers were used for PCR amplification by using gradient annealing temperatures and orthogonal design method, and genetic genetic relationship among clones was researched by using UPGMA clustering method. The results showed that target bands were amplified by 12 pairs of primers, and 214 alleles were obtained at 12 SSR loci. Based on amplified band types of each clone by 12 pairs of primers, genetic similarity coefficients ranged from 0.62 to 0.95 between clones, and average similarity coefficient was 0.793. The results of cluster analysis showed that the clones with closely geographical origins were closely clustered together. SSR markers showed that the phylogenetic relationship among Camellia germplasms was not completely consistent with the classification results of morphology.
引文
[1]张景荣.名贵茶花种质资源的RAPD分析[D].雅安:四川农业大学,2006.
[2]张宏达.茶树的系统分类[J].中山大学学报(自然科学版),1981(1):89-101.
[3]胡兴华,王燕,邹玲俐,等.茶花品种SSR指纹图谱分型技术反应体系优化[J].中国农学通报,2013,29(1):127-131.
[4]田晔林,刘克旺.湖南省山茶属红山茶组植物资源及园林观赏特性的研究[J].中国农学通报,2004,20(3):193-195.
[5]张常乐,丁绍刚,李升科.山茶属植物在园林绿化中的应用[J].江苏农业科学,2011,39(6):314-316.
[6]赵松子.山茶属植物转录组文献分析[J].南方林业科学,2016,44(4):13-16.
[7]陈金磊,沈宝涛,杨柏云.不同处理对油茶芽苗根接茶花成活率的影响[J].湖北农业科学,2017,56(15):2904-2907.
[8]刘海龙,马锦林,张日清,等.11份山茶属植物亲缘关系的ISSR分析[J].经济林研究,2012,30(4):87-90.
[9]闻丽,张日清,李建安,等.油茶种质改良现状及其花药培养技术的应用前景[J].经济林研究,2004,22(4):87-90.
[10]WANG L,WANG X C,YUE C,et al.Development of a 44Kcustom oligo microarray using 454 pyrosequencing data for large-scale gene expression analysis of Camellia sinensis[J].Scientia Horticulturae,2014:174.
[11]罗春清,谭晓风,漆龙霖.山茶属植物分类综述[J].中南林学院学报,1999,19(3):78-81.
[12]黄永芳,陈锡沐,庄雪影,等.油茶种质资源遗传多样性分析[J].林业科学,2006,42(4):38-43.
[13]王瑞,陈隆升,彭映赫,等.油茶新品种‘晚霞’嫁接亲和性研究[J].中南林业科技大学学报,2018,38(3):9-12.
[14]NAGHMA KHAN,HASAN MUKHTAR.Tea polyphenols for health promotion[J].Life Sciences,2007,81(7):519-533.
[15]俞明亮,马瑞娟,许建兰,等.桃种间亲缘关系的SSR鉴定[J].果树学报,2004,21(2):106-112.
[16]柳晓磊,汤华,李东栋,等.海南椰子栽培品种的SSR标记分析[J].园艺学报,2008,35(8):1199-1204.
[17]彭继庆,曹福祥,范海燕.博白大果油茶遗传多样性的ISSR研究[J].中南林业科技大学学报,2013,33(7):62-66.
[18]邓白罗,谭晓风,漆龙霖,等.山茶属红山茶组植物的RAPD分析及分类研究[J].林业科学,2006,42(5):36-41.
[19]余智城,何雪娇,曹明华.基于表型性状和SSR标记的20份香蕉材料遗传多样性研究[J].中国南方果树,2014,43(3):10-14.
[20]温贝贝,邓丽,叶霞,等.DNA条形码在山茶属近缘植物鉴别中的应用[J].广东农业科学,2017,44(1):55-65.
[21]谢荟,祝文娟,张应中,等.基于SRAP分子标记的广宁红花油茶种质资源遗传多样性研究[J].中南林业科技大学学报,2017,37(8):93-97.
[22]杜忠,曾彦,李秋凤.油茶嫁接山茶花的技术[J].广西林业科学,2008,37(3):161-163.
[23]梁英荣.成年油茶嫁接山茶花技术[J].特种经济动植物,2006,9(2):36.
[24]张力,庄志勇,黄国林,等.油茶树桩改接茶花技术研究[J].安徽农业科学,2017,45(16):151-152.
[25]韩国勇.油茶老树嫁接茶花快速繁育茶花大苗技术初探[J].林业勘察设计,2006(2):211-214.
[26]张鹏飞,尉东峰,刘亚令,等.枣与酸枣的SSR遗传多样性研究[J].华北农学报,2015,30(2):150-155.
[2]张宏达.茶树的系统分类[J].中山大学学报(自然科学版),1981(1):89-101.
[3]胡兴华,王燕,邹玲俐,等.茶花品种SSR指纹图谱分型技术反应体系优化[J].中国农学通报,2013,29(1):127-131.
[4]田晔林,刘克旺.湖南省山茶属红山茶组植物资源及园林观赏特性的研究[J].中国农学通报,2004,20(3):193-195.
[5]张常乐,丁绍刚,李升科.山茶属植物在园林绿化中的应用[J].江苏农业科学,2011,39(6):314-316.
[6]赵松子.山茶属植物转录组文献分析[J].南方林业科学,2016,44(4):13-16.
[7]陈金磊,沈宝涛,杨柏云.不同处理对油茶芽苗根接茶花成活率的影响[J].湖北农业科学,2017,56(15):2904-2907.
[8]刘海龙,马锦林,张日清,等.11份山茶属植物亲缘关系的ISSR分析[J].经济林研究,2012,30(4):87-90.
[9]闻丽,张日清,李建安,等.油茶种质改良现状及其花药培养技术的应用前景[J].经济林研究,2004,22(4):87-90.
[10]WANG L,WANG X C,YUE C,et al.Development of a 44Kcustom oligo microarray using 454 pyrosequencing data for large-scale gene expression analysis of Camellia sinensis[J].Scientia Horticulturae,2014:174.
[11]罗春清,谭晓风,漆龙霖.山茶属植物分类综述[J].中南林学院学报,1999,19(3):78-81.
[12]黄永芳,陈锡沐,庄雪影,等.油茶种质资源遗传多样性分析[J].林业科学,2006,42(4):38-43.
[13]王瑞,陈隆升,彭映赫,等.油茶新品种‘晚霞’嫁接亲和性研究[J].中南林业科技大学学报,2018,38(3):9-12.
[14]NAGHMA KHAN,HASAN MUKHTAR.Tea polyphenols for health promotion[J].Life Sciences,2007,81(7):519-533.
[15]俞明亮,马瑞娟,许建兰,等.桃种间亲缘关系的SSR鉴定[J].果树学报,2004,21(2):106-112.
[16]柳晓磊,汤华,李东栋,等.海南椰子栽培品种的SSR标记分析[J].园艺学报,2008,35(8):1199-1204.
[17]彭继庆,曹福祥,范海燕.博白大果油茶遗传多样性的ISSR研究[J].中南林业科技大学学报,2013,33(7):62-66.
[18]邓白罗,谭晓风,漆龙霖,等.山茶属红山茶组植物的RAPD分析及分类研究[J].林业科学,2006,42(5):36-41.
[19]余智城,何雪娇,曹明华.基于表型性状和SSR标记的20份香蕉材料遗传多样性研究[J].中国南方果树,2014,43(3):10-14.
[20]温贝贝,邓丽,叶霞,等.DNA条形码在山茶属近缘植物鉴别中的应用[J].广东农业科学,2017,44(1):55-65.
[21]谢荟,祝文娟,张应中,等.基于SRAP分子标记的广宁红花油茶种质资源遗传多样性研究[J].中南林业科技大学学报,2017,37(8):93-97.
[22]杜忠,曾彦,李秋凤.油茶嫁接山茶花的技术[J].广西林业科学,2008,37(3):161-163.
[23]梁英荣.成年油茶嫁接山茶花技术[J].特种经济动植物,2006,9(2):36.
[24]张力,庄志勇,黄国林,等.油茶树桩改接茶花技术研究[J].安徽农业科学,2017,45(16):151-152.
[25]韩国勇.油茶老树嫁接茶花快速繁育茶花大苗技术初探[J].林业勘察设计,2006(2):211-214.
[26]张鹏飞,尉东峰,刘亚令,等.枣与酸枣的SSR遗传多样性研究[J].华北农学报,2015,30(2):150-155.