EMS诱变马铃薯抗寒突变体筛选
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摘要
【目的】定向选择马铃薯抗寒突变体。【方法】以马铃薯品种费乌瑞它为材料,用甲基磺酸乙酯(EMS)诱变处理费乌瑞它愈伤组织,结合添加L-羟脯氨酸(L-Hyp)培养筛选,定向选择抗寒突变体。【结果】诱变费乌瑞它愈伤组织的最佳EMS浓度是0.8%,处理时间4 h;L-Hyp最佳浓度为0.5%。经检测诱变后代再生苗脯氨酸含量明显高出对照,而电解质渗出率均低于对照。【结论】用EMS诱变处理马铃薯愈伤组织可获得抗寒突变体。
【Objective】This paper aimed to obtain the cold-resistant variant.【Method】The Favorita potato test-tube plantlet callus could be as materials, then the callus was induced to produce variant by mutagen EMS combination with selection induced callus on L-hydroxyproline(L-Hyp). 【Result】The best treatment composition of EMS was 0.8 % for 4 hours and the best selecting composition of L-Hyp was 0.5 %. Compared to control, the resistant variation and its regenerated plantlet remained characteristic of high proline and low osmotic stress-resisting. 【Conclusion】The cold-resistant variant on potato could be obtained by EMS inducing treatment.
【Objective】This paper aimed to obtain the cold-resistant variant.【Method】The Favorita potato test-tube plantlet callus could be as materials, then the callus was induced to produce variant by mutagen EMS combination with selection induced callus on L-hydroxyproline(L-Hyp). 【Result】The best treatment composition of EMS was 0.8 % for 4 hours and the best selecting composition of L-Hyp was 0.5 %. Compared to control, the resistant variation and its regenerated plantlet remained characteristic of high proline and low osmotic stress-resisting. 【Conclusion】The cold-resistant variant on potato could be obtained by EMS inducing treatment.
引文
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[7]李飞,金黎平.马铃薯耐霜冻研究进展[J].贵州农业科学,2007,35(4):140-142.
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[10]夏曾润,杜凤凤,李偲,等.紫花苜宿EMS突变体库的构建和形态学性状鉴定[J].草业学报,2014,23(2):215-222.
[2]柳永强,胡新元,李掌,等.EMS诱导四倍体马铃薯陇薯7号无性突变群体建立与分析[J].农业科学与技术,2016,17(8):1780-1785.
[3]王淼,马晓梅,郭晋.马铃薯茎段60Co-r射线及EMS诱变技术的研究[J].安徽农信通报,2010:16(3):45-46.
[4]杨乾,张峰,王蒂,等.EMS诱变马铃薯茎段离体奶盐突变体[J].核农学报,25(4):673-678.
[5]门福义,刘梦芸.马铃薯栽培生理[M].北京:中国农业出版社,1995.
[6]李芳.马铃薯耐霜冻能力鉴定以及低温处理下表达谱分析[D]. 武汉:华中农业大学,2011.
[7]李飞,金黎平.马铃薯耐霜冻研究进展[J].贵州农业科学,2007,35(4):140-142.
[8]Van Harten A M.Mutation breeding[M].London:Cambridge University Press,1998.
[9]罗海波,周颉,贺从安,等.甲基磺酸乙酯(EMS)对萝卜早期发育性状的诱变效应[J].基因组学与应用生物学,2013,32(5):608-611.
[10]夏曾润,杜凤凤,李偲,等.紫花苜宿EMS突变体库的构建和形态学性状鉴定[J].草业学报,2014,23(2):215-222.