绿薄荷精油对温和气单胞菌群体感应现象及其腐败特性的抑制作用
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摘要
本研究以紫色杆菌Chromobacterium violaceum 026(CV026)为检测指标,结合气相色谱-质谱定量检测信号分子,研究绿薄荷精油对温和气单胞菌群体感应现象及其腐败特性的影响。结果表明:在亚抑菌剂量下,绿薄荷精油具有降低紫色杆菌CV026产紫色菌素的能力,其剂量为2μL/mL时,对紫色菌素的抑制率达56.17%;绿薄荷精油对温和气单胞菌N-酰基高丝氨酸内酯的分泌及某些腐败特性的表达,如生物被膜的形成、胞外蛋白酶活力和细菌迁移(群集和泳动),均有抑制作用;随着绿薄荷精油剂量的升高,抑制效果更趋明显,并且呈现剂量依赖性。可见,绿薄荷精油具有良好的群体感应抑制活性,可作为新型的群体感应抑制剂用于延长水产品货架期。
In this paper, the effect of spearmint oil on quorum sensing and spoilage characteristics of Aeromonas sobria was studied. For this purpose, we firstly assessed the inhibitory impact of spearmint oil on the violacein-producing ability of Chromobacterium violaceum 026(CV026). The signal molecule was detected by gas chromatography-mass spectrometry(GC-MS). The results showed that spearmint oil could reduce the violacein production of CV026 at sub-minimum inhibitory concentrations. When the concentration of spearmint oil was 2 μL/mL, the inhibitory rate of violacein was 56.17%. The secretion of N-acyl-homoserine lactones(AHLs) and some spoilage characteristics such as biofilm formation, extracellular protease activity and bacterial motility of Aeromonas sobria were inhibited by spearmint oil effectively and dose-dependently. These results showed that spearmint oil could be developed as a quorum sensing inhibitor to enhance the shelf life of aquatic products.
In this paper, the effect of spearmint oil on quorum sensing and spoilage characteristics of Aeromonas sobria was studied. For this purpose, we firstly assessed the inhibitory impact of spearmint oil on the violacein-producing ability of Chromobacterium violaceum 026(CV026). The signal molecule was detected by gas chromatography-mass spectrometry(GC-MS). The results showed that spearmint oil could reduce the violacein production of CV026 at sub-minimum inhibitory concentrations. When the concentration of spearmint oil was 2 μL/mL, the inhibitory rate of violacein was 56.17%. The secretion of N-acyl-homoserine lactones(AHLs) and some spoilage characteristics such as biofilm formation, extracellular protease activity and bacterial motility of Aeromonas sobria were inhibited by spearmint oil effectively and dose-dependently. These results showed that spearmint oil could be developed as a quorum sensing inhibitor to enhance the shelf life of aquatic products.
引文
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[19]VIJAYARAGHAVAN P,VINCENT S G P.A simple method for the detection of protease activity on agar plates using bromocresolgreen dye[J].Journal of Biochemical Technology,2013,4(3):628-630.
[20]ZHANG J M,RUI X,WANG L,et al.Polyphenolic extract from Rosa rugosa tea inhibits bacterial quorum sensing and biofilm formation[J].Food Control,2014,42(2):125-131.DOI:10.1016/j.foodcont.2014.02.001.
[21]LI T T,CUI F C,BAI F L,et al.Involvement of acylated homoserine lactones(AHLs)of Aeromonas sobria in spoilage of refrigerated turbot(Scophthalmus maximus L.)[J].Sensors,2016,16(7):2-11.DOI:10.3390/s16071083.
[22]KUMAR C G,ANAND S K.Significance of microbial biofilms in food industry:a review[J].International Journal of Food Microbiology,1998,42(1/2):9-27.DOI:10.1016/S0168-1605(98)00060-9.
[23]ASWATHANARAYAN J B,VITTAL R.R.Quorum sensing inhibitory and anti-biofilm activity of essential oils and their in vivo efficacy in food systems[J].Food Biotechnology,2014,28:269-292.
[24]LAMBROPOULOU K A,DROSINOS E H,NYCHAS G J.The effect of glucose supplementation on the spoilage microflora and chemical composition of minced beef stored aerobically or under a modified atmosphere at 4℃[J].International Journal of Food Microbiology,1996,30(3):281-291.DOI:10.1016/0168-1605(96)00954-3.
[25]葛静慧,崔玉娜,王晶,等.即食海参优势腐败菌群体感应信号分子识别[J].中国食品学报,2015,15(11):186-187.DOI:10.16429/j.1009-7848.2015.11.028.
[26]赵丽珺,谢晶,冯淑娇,等.冷却猪肉中产蛋白酶腐败菌的分离鉴定[J].食品工业科技,2014,35(7):134-139;143.
[27]赵龙华,杨维青.细菌群集运动与生物被膜和耐药性的关系[J].国际检验医学杂志,2011,32(17):1986-1988.DOI:10.3969/j.issn.1673-4130.2011.17.032.
[28]O’MAY C,TUFENKJI N.The swarming motility of Pseudomonas aeruginosa is blocked by cranberry proanthocyanidins and other tannin-containing materials[J].Applied and Environmental Microbiology,2011,77(9):3061-3067.DOI:10.1128/AEM.02677-10.
[29]MERRITT J H,BROTHERS K M,KUCHMA S L,et al.SadC reciprocally influences biofilm formation and swarming motility via modulation of exopolysaccharide production and flagellar function[J].Journal of Bacteriology,2007,189(22):8154-8164.DOI:10.1128/JB.00585-07.
[30]DANIELS R,VANDERLEYDEN J,MICHIELS J.Quorum sensing and swarming migration in bacteria[J].FEMS Microbiology Reviews,2004,28(3):261-289.
[2]BHARATI B K,CHATTERJI D.Quorum sensing and pathogenesis:role of small signalling molecules in bacterial persistence[J].Current Science,2013,105(5):643-656.
[3]AMMOR M S,MICHAELIDIS C,NYCHAS G J E.Insights into the role of quorum sensing in food spoilage[J].Journal of Food Protection,2008,71(7):1510-1525.DOI:10.4315/0362-028X-71.7.1510.
[4]CHAN X Y,HOW K Y,YIN W F,et al.N-Acylhomoserine lactonemediated quorum sensing in Aeromonas veronii biovar sobria strain159:identification of LuxRI homologs[J].Frontiers in Cellular and Infection Microbiology,2016,6:1-6.DOI:10.3389/fcimb.2016.00007.
[5]励建荣,杨兵,李婷婷.水产品优势腐败菌及其群体感应系统研究进展[J].食品科学,2015,36(19):255-259.DOI:10.7506/spkx1002-6630-201519046.
[6]MINAGAWA S,INAMI H,KATO T,et al.RND type efflux pump system MexAB-OprM of Pseudomonas aeruginosa selects bacterial languages,3-oxo-acyl-homoserine lactones,for cell-to-cell communication[J].BMC Microbiology,2012,12:70.
[7]KIM M H,CHOI W C,KANG H O,et al.The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(49):17606-17611.DOI:10.1073/pnas.0504996102.
[8]VENKADESAPERUMAL G,RUCHA S,SUNDAR K,et al.Antiquorum sensing activity of spice oil nanoemulsions against food borne pathogens[J].LWT-Food Science and Technology,2016,66:225-231.DOI:10.1016/j.lwt.2015.10.044.
[9]SEPAHI E,TARIGHI S,AHMADI F S,et al.Inhibition of quorum sensing in Pseudomonas aeruginosa by two herbal essential oils from Apiaceae family[J].Journal of Microbiology,2015,53(2):1-4.DOI:10.1007/s12275-015-4203-8.
[10]MYSZKA K,SCHMIDT M T,MAJCHER M,et al.Inhibition of quorum sensing-related biofilm of Pseudomonas fluorescens KM121by Thymus vulgare essential oil and its major bioactive compounds[J].International Biodeterioration&Biodegradation,2016,114:252-259.DOI:10.1016/j.ibiod.2016.07.006.
[11]LAWRENCE B M.Scotch spearmint oil progress in essential oil[J].Perfumer&Flavorist,1993,18(3):61-64.
[12]钟黎,魏金凤.留兰香油的提取及应用研究[J].食品研究与开发,2003(4):26-28.
[13]盖旭,李荣,姜子涛.调味香料留兰香精油的研究进展[J].中国调味品,2012,37(1):80-83;107.
[14]潘旭迟,傅昱晟,许剑锋,等.17种植物挥发油对食源微生物抑菌活性研究[J].食品工业科技,2016,37(16):107-112.DOI:10.13386/j.issn1002-0306.2016.16.013.
[15]梁巧雯,关宝容,李泽洪,等.留兰香精油提取副产物对鲜切生菜保鲜效果的影响[J].农产品加工,2015(8):7-9.DOI:10.3969/jissn.1671-9646(X).2015.04.033.
[16]班斓,孙雁,黄生树.留兰香提取物延缓牛奶氧化的研究[J].中国食品添加剂,2016(4):139-149.
[17]ADONIZIO A L,DOWNUM K,BENNETT B C,et al.Anti-quorum sensing activity of medicinal plants in Southern Florida[J].Journal of Ethnopharmacology,2006,105(3):427-435.DOI:10.1016/j.jep.2005.11.025.
[18]RODE T M,LANGSRUD S,HOLCK A,et al.Different patterns of biofilm formation in Staphylococcus aureus under food-related stress conditions[J].International Journal of Food Microbiology,2007,116:372-383.
[19]VIJAYARAGHAVAN P,VINCENT S G P.A simple method for the detection of protease activity on agar plates using bromocresolgreen dye[J].Journal of Biochemical Technology,2013,4(3):628-630.
[20]ZHANG J M,RUI X,WANG L,et al.Polyphenolic extract from Rosa rugosa tea inhibits bacterial quorum sensing and biofilm formation[J].Food Control,2014,42(2):125-131.DOI:10.1016/j.foodcont.2014.02.001.
[21]LI T T,CUI F C,BAI F L,et al.Involvement of acylated homoserine lactones(AHLs)of Aeromonas sobria in spoilage of refrigerated turbot(Scophthalmus maximus L.)[J].Sensors,2016,16(7):2-11.DOI:10.3390/s16071083.
[22]KUMAR C G,ANAND S K.Significance of microbial biofilms in food industry:a review[J].International Journal of Food Microbiology,1998,42(1/2):9-27.DOI:10.1016/S0168-1605(98)00060-9.
[23]ASWATHANARAYAN J B,VITTAL R.R.Quorum sensing inhibitory and anti-biofilm activity of essential oils and their in vivo efficacy in food systems[J].Food Biotechnology,2014,28:269-292.
[24]LAMBROPOULOU K A,DROSINOS E H,NYCHAS G J.The effect of glucose supplementation on the spoilage microflora and chemical composition of minced beef stored aerobically or under a modified atmosphere at 4℃[J].International Journal of Food Microbiology,1996,30(3):281-291.DOI:10.1016/0168-1605(96)00954-3.
[25]葛静慧,崔玉娜,王晶,等.即食海参优势腐败菌群体感应信号分子识别[J].中国食品学报,2015,15(11):186-187.DOI:10.16429/j.1009-7848.2015.11.028.
[26]赵丽珺,谢晶,冯淑娇,等.冷却猪肉中产蛋白酶腐败菌的分离鉴定[J].食品工业科技,2014,35(7):134-139;143.
[27]赵龙华,杨维青.细菌群集运动与生物被膜和耐药性的关系[J].国际检验医学杂志,2011,32(17):1986-1988.DOI:10.3969/j.issn.1673-4130.2011.17.032.
[28]O’MAY C,TUFENKJI N.The swarming motility of Pseudomonas aeruginosa is blocked by cranberry proanthocyanidins and other tannin-containing materials[J].Applied and Environmental Microbiology,2011,77(9):3061-3067.DOI:10.1128/AEM.02677-10.
[29]MERRITT J H,BROTHERS K M,KUCHMA S L,et al.SadC reciprocally influences biofilm formation and swarming motility via modulation of exopolysaccharide production and flagellar function[J].Journal of Bacteriology,2007,189(22):8154-8164.DOI:10.1128/JB.00585-07.
[30]DANIELS R,VANDERLEYDEN J,MICHIELS J.Quorum sensing and swarming migration in bacteria[J].FEMS Microbiology Reviews,2004,28(3):261-289.