新疆不同棉花品种(系)对土耳其斯坦叶螨抗性的鉴定及其叶片表面蜡质分析
|
摘要
【目的】以新疆棉区代表性的236份棉花种质为材料,对其棉叶螨的抗性进行鉴定,并分析叶片蜡质含量及蜡质组分,为棉花抗螨育种奠定基础。【方法】在室内于棉花苗期分批次进行接螨鉴定,同时测定不同棉花材料叶片的蜡质含量,并利用气相色谱-质谱法分析叶片的蜡质组分。【结果】大部分棉花材料对棉叶螨的危害敏感,236份棉花材料中有高抗品系2份、抗性材料3份、中抗材料22份、敏感材料207份、高感材料2份。棉叶表面蜡质含量与叶片受害级数存在显著负相关;棉花叶片蜡质成分主要为烷烃类化合物,含有少量的酯类、醚类、脂肪酸等。高抗螨棉花种质材料叶片蜡质中烷烃类与酯类组分的含量均高于高感螨材料。高抗和高感材料中含量差距最大的化合物有C24H50、C35H72、C17H36。【结论】上述研究结果为下一步研究棉花表面蜡质组分对棉叶螨寄主选择和抗螨作用的影响奠定了基础。
[Objective] Based on the representative 236 cotton varieties in Xinjiang, the resistance of cotton to mites was identified, and the waxy content and waxy components of leaves were analyzed to lay a foundation for cotton breeding.[Method] In the cotton seedling stage, the waxy content of the leaves of different cotton varieties was determined, and the waxy components of the leaves were analyzed by gas chromatography-mass spectrometry. [Result] Most cotton varieties are sensitive to the damage of cotton mites. There were 2 high resistance varieties, 3 resistant materials, 22 medium resistant materials, 207 sensitive materials and 2 high-sensitivity materials in 236 cotton materials. The waxy content on the surface of cotton leaves was significantly negatively correlated with leaf damage levels. The waxy components of cotton leaves were mainly alkane compounds, containing a small amount of esters, ethers and fatty acids. The content of alkanes and esters in waxy leaves of high-resistance cotton germplasm materials was higher than that of high-sensitivity materials. The compound with the largest difference in content are C24 H50, C35 H72 and C17 H36. [Conclusion] These results lay a foundation for further study on the effects of waxy content on host selection and mite resistance of cotton mites.
[Objective] Based on the representative 236 cotton varieties in Xinjiang, the resistance of cotton to mites was identified, and the waxy content and waxy components of leaves were analyzed to lay a foundation for cotton breeding.[Method] In the cotton seedling stage, the waxy content of the leaves of different cotton varieties was determined, and the waxy components of the leaves were analyzed by gas chromatography-mass spectrometry. [Result] Most cotton varieties are sensitive to the damage of cotton mites. There were 2 high resistance varieties, 3 resistant materials, 22 medium resistant materials, 207 sensitive materials and 2 high-sensitivity materials in 236 cotton materials. The waxy content on the surface of cotton leaves was significantly negatively correlated with leaf damage levels. The waxy components of cotton leaves were mainly alkane compounds, containing a small amount of esters, ethers and fatty acids. The content of alkanes and esters in waxy leaves of high-resistance cotton germplasm materials was higher than that of high-sensitivity materials. The compound with the largest difference in content are C24 H50, C35 H72 and C17 H36. [Conclusion] These results lay a foundation for further study on the effects of waxy content on host selection and mite resistance of cotton mites.
引文
[1]李雪源,王俊铎,郑巨云,等.新疆棉花产业发展与供给侧改革[J].中国棉花, 2017, 44(8):1-7.Li Xueyuan, Wang Junduo, Zheng Juyun, et al. Xinjiang cotton industry development and supply-side reform[J]. China Cotton,2017, 44(08):1-7.
[2] Park Y L, Lee J H. Leaf cell and tissue damage of cucumber caused by two spotted spider mite(Acari:Tetranychidae)[J].Journal of Economic Entomology, 2002, 95(5):952-957. https://doi.org/10.1093/jee/95.5.952
[3]周小云,成湘,马盾,等.北疆棉田主要有害生物综合防治措施田[J].棉花科学, 2012, 34(1):52-54.Zhou Xiaoyun, Cheng Xiang, Ma Dun, et al. Main pest control measures in cotton fields in northern Xinjiang[J]. Cotton Sciences, 2012, 34(1):52-54.
[4]王美芳,陈巨莲,原国辉,等.植物表面蜡质对植食性昆虫的影响研究进展[J].生态环境学报, 2009, 18(3):1155-1160.Wang Meifang, Chen Julian, Yuan Guohui, et al. Advances in research on the effects of plant surface wax on phytophagous insects[J]. Ecology and Environment Sciences, 2009, 18(3):1155-1160.
[5]马纲,张敏.植物抗虫性物质及作用的多样性[J].生物学通报,2002, 37(12):8-9.Ma Gang, Zhang Min. Diversity of plant insect-resistant substances and their effects[J]. Biological Bulletin, 2002, 37(12):8-9.
[6]张文辉,刘光杰.植物抗虫性次生物质的研究概况[J].植物学通报, 2003, 20(5):522-530.Zhang Wenhui, Liu Guangjie. Research overview of plant insect-resistant secondary substances[J]. Botanical Bulletin, 2003,20(5):522-530.
[7]杜雄明,孙君灵,周忠丽,等.棉花资源收集、保存、评价与利用现状及未来[J].植物遗传资源学报, 2012, 13(2):163-168.Du Xiongming, Sun Junling, Zhou Zhongli, et al. Status and future of cotton resources collection, conservation, evaluation and utilization[J]. Journal of Plant Genetic Resources, 2012, 13(2):163-168.
[8] Jetter R, Schaffer S. Chemical composition of the Prunus laurocerasus leaf surface, dynamic changes of the epicuticular wax film during leaf development[J]. Plant Physiology, 2001, 126(4):1725-1737. https://doi.org/10.1104/pp.126.4.1725
[9]耿文超,赵伊英,刘峰,等.同步抑制棉叶螨TtAK与TtCHI基因表达的RNAi载体的构建[J].西北农业学报, 2016, 25(4):554-560.Geng Wenchao, Zhao Yiying, Liu Feng, et al. Construction of RNAi vector for simultaneous inhibition of TtAK and TtCHI gene expression in cotton mites[J]. Northwest Agricultural Journal, 2016, 25(4):554-560.
[10]张金发,孙济中,吴征彬,等.棉花对朱砂叶螨抗性的鉴定和机制研究[J].植物保护学报, 1993, 20(2):155-161.Zhang Jinfa, Sun Jizhong, Wu Zhengbin, et al. Identification and mechanism of cotton resistance to Tetranychus cinnabarinus[J]. Journal of Plant Protection, 1993, 20(2):155-161.
[11]王朝生,杨刚,董顺文,等.抗棉叶螨棉花种质川98系的选育[J].中国农业科学, 1991, 24(4):32-40.Wang Chaosheng, Yang Gang, Dong Shunwen, et al. Breeding of Chuan 98 line, a cotton mites resistant germplasm[J]. Agricultural Science of China, 1991, 24(4):32-40.
[12]雍小菊,丁伟.植物的抗螨机理[J].应用昆虫学报, 2011, 48(5):1495-1504.Yong Xiaoju, Ding Wei. The mechanism of mite resistance of plants[J]. Journal of Applied Entomology, 2011, 48(5):1495-1504.
[13]袁辉霞.土耳其斯坦叶螨对棉花不同品种(系)的寄主选择性及其机理研究[D].石河子:石河子大学, 2009.Yuan Huixia. Host selection and mechanism of Tetranychus turkestani to different cotton varieties[D]. Shihezi:Shihezi University, 2009.
[14] Riederer M, Schreiber L. Protecting against water loss:Analysis of the barrier properties of plant cuticles[J]. Journal of Experimental Botany, 2001, 52(363):2023-2032. https://doi.org/10.1093/jexbot/52.363.2023
[15] Daoust S P, Mader B J, Bauce E, et al. Influence of epicuticular-wax composition on the feeding pattern of a phytophagous insect:implications for host resistance[J]. The Canadian Entomologist, 2010, 142(3):261-270. https://doi. org/10. 4039/n09-064
[16] Eigenbrode S D, Espelie K E. Effects of plant epicuticular lipids on insect herbivores[J]. Annual Review of Entomology, 1995,40(1):171-194. https://doi.org/10.1146/annurev.en.40.010195.001131
[17]刘勇,陈巨莲,程登发.不同小麦品种(系)叶片表面蜡质对两种麦蚜取食的影响[J].应用生态学报, 2007, 18(8):1785-1788.Liu Yong, Chen Julian, Cheng Dengfa. Effects of leaf wax on the feeding of two wheat aphids in different wheat varieties(lines)[J]. Journal of Applied Ecology, 2007, 18(8):1785-1788.
[18] Yang M G, Yang Q Y, Fu T D, et al. Overexpression of the Brassica napes BnLAS gene in Arabidopsis affects plant development and increases drought tolerance[J]. Plant Cell Reports,2011, 30(3):373-388. https://doi.org/10.1007/s00299-010-0940-7
[2] Park Y L, Lee J H. Leaf cell and tissue damage of cucumber caused by two spotted spider mite(Acari:Tetranychidae)[J].Journal of Economic Entomology, 2002, 95(5):952-957. https://doi.org/10.1093/jee/95.5.952
[3]周小云,成湘,马盾,等.北疆棉田主要有害生物综合防治措施田[J].棉花科学, 2012, 34(1):52-54.Zhou Xiaoyun, Cheng Xiang, Ma Dun, et al. Main pest control measures in cotton fields in northern Xinjiang[J]. Cotton Sciences, 2012, 34(1):52-54.
[4]王美芳,陈巨莲,原国辉,等.植物表面蜡质对植食性昆虫的影响研究进展[J].生态环境学报, 2009, 18(3):1155-1160.Wang Meifang, Chen Julian, Yuan Guohui, et al. Advances in research on the effects of plant surface wax on phytophagous insects[J]. Ecology and Environment Sciences, 2009, 18(3):1155-1160.
[5]马纲,张敏.植物抗虫性物质及作用的多样性[J].生物学通报,2002, 37(12):8-9.Ma Gang, Zhang Min. Diversity of plant insect-resistant substances and their effects[J]. Biological Bulletin, 2002, 37(12):8-9.
[6]张文辉,刘光杰.植物抗虫性次生物质的研究概况[J].植物学通报, 2003, 20(5):522-530.Zhang Wenhui, Liu Guangjie. Research overview of plant insect-resistant secondary substances[J]. Botanical Bulletin, 2003,20(5):522-530.
[7]杜雄明,孙君灵,周忠丽,等.棉花资源收集、保存、评价与利用现状及未来[J].植物遗传资源学报, 2012, 13(2):163-168.Du Xiongming, Sun Junling, Zhou Zhongli, et al. Status and future of cotton resources collection, conservation, evaluation and utilization[J]. Journal of Plant Genetic Resources, 2012, 13(2):163-168.
[8] Jetter R, Schaffer S. Chemical composition of the Prunus laurocerasus leaf surface, dynamic changes of the epicuticular wax film during leaf development[J]. Plant Physiology, 2001, 126(4):1725-1737. https://doi.org/10.1104/pp.126.4.1725
[9]耿文超,赵伊英,刘峰,等.同步抑制棉叶螨TtAK与TtCHI基因表达的RNAi载体的构建[J].西北农业学报, 2016, 25(4):554-560.Geng Wenchao, Zhao Yiying, Liu Feng, et al. Construction of RNAi vector for simultaneous inhibition of TtAK and TtCHI gene expression in cotton mites[J]. Northwest Agricultural Journal, 2016, 25(4):554-560.
[10]张金发,孙济中,吴征彬,等.棉花对朱砂叶螨抗性的鉴定和机制研究[J].植物保护学报, 1993, 20(2):155-161.Zhang Jinfa, Sun Jizhong, Wu Zhengbin, et al. Identification and mechanism of cotton resistance to Tetranychus cinnabarinus[J]. Journal of Plant Protection, 1993, 20(2):155-161.
[11]王朝生,杨刚,董顺文,等.抗棉叶螨棉花种质川98系的选育[J].中国农业科学, 1991, 24(4):32-40.Wang Chaosheng, Yang Gang, Dong Shunwen, et al. Breeding of Chuan 98 line, a cotton mites resistant germplasm[J]. Agricultural Science of China, 1991, 24(4):32-40.
[12]雍小菊,丁伟.植物的抗螨机理[J].应用昆虫学报, 2011, 48(5):1495-1504.Yong Xiaoju, Ding Wei. The mechanism of mite resistance of plants[J]. Journal of Applied Entomology, 2011, 48(5):1495-1504.
[13]袁辉霞.土耳其斯坦叶螨对棉花不同品种(系)的寄主选择性及其机理研究[D].石河子:石河子大学, 2009.Yuan Huixia. Host selection and mechanism of Tetranychus turkestani to different cotton varieties[D]. Shihezi:Shihezi University, 2009.
[14] Riederer M, Schreiber L. Protecting against water loss:Analysis of the barrier properties of plant cuticles[J]. Journal of Experimental Botany, 2001, 52(363):2023-2032. https://doi.org/10.1093/jexbot/52.363.2023
[15] Daoust S P, Mader B J, Bauce E, et al. Influence of epicuticular-wax composition on the feeding pattern of a phytophagous insect:implications for host resistance[J]. The Canadian Entomologist, 2010, 142(3):261-270. https://doi. org/10. 4039/n09-064
[16] Eigenbrode S D, Espelie K E. Effects of plant epicuticular lipids on insect herbivores[J]. Annual Review of Entomology, 1995,40(1):171-194. https://doi.org/10.1146/annurev.en.40.010195.001131
[17]刘勇,陈巨莲,程登发.不同小麦品种(系)叶片表面蜡质对两种麦蚜取食的影响[J].应用生态学报, 2007, 18(8):1785-1788.Liu Yong, Chen Julian, Cheng Dengfa. Effects of leaf wax on the feeding of two wheat aphids in different wheat varieties(lines)[J]. Journal of Applied Ecology, 2007, 18(8):1785-1788.
[18] Yang M G, Yang Q Y, Fu T D, et al. Overexpression of the Brassica napes BnLAS gene in Arabidopsis affects plant development and increases drought tolerance[J]. Plant Cell Reports,2011, 30(3):373-388. https://doi.org/10.1007/s00299-010-0940-7
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |